LOCUS       RC207975                7278 bp    DNA     circular  SYN 
DEFINITION  ZNF227 (Myc-DDK-tagged)-Human zinc finger protein 227 (ZNF227)
ACCESSION   NM_182490
VERSION     NM_182490.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3435..3526 
                     /label="Myc-DDK"  
     misc_feature    3556..4141 
                     /label="PolyA signal"  
     misc_feature    3613..3632 
                     /label="Restriction Enzyme"  
     misc_feature    4417..5089 
                     /label="ColE1"  
     misc_feature    5519..6313 
                     /label="Kan/Neo"  
     misc_feature    6348..6650 
                     /label="SV40 ori"  
     misc_feature    6736..7213 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3417..3423 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3417 
                     /label="ZNF227"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCATCTCAG AACTATGACC TTCCCCAGAA GAAGCAGGAG AAAATGACCA 
     1081 AGTTTCAGGA GGCTGTGACA TTCAAGGATG TGGCTGTGGT CTTCTCCAGG GAGGAACTGC 
     1141 GACTGCTCGA TCTTACCCAG AGGAAGCTGT ACCGAGATGT CATGGTGGAG AACTTCAAGA 
     1201 ACCTGGTTGC AGTGGGGCAT CTTCCCTTCC AACCAGATAT GGTATCCCAA TTGGAAGCAG 
     1261 AAGAAAAGCT TTGGATGATG GAAACAGAAA CCCAAAGAAG CAGCAAGCAT CAAAATAAGA 
     1321 TGGAAACACT CCAAAAATTT GCATTAAAAT ACCTTTCAAA TCAAGAGCTG TCCTGCTGGC 
     1381 AAATCTGGAA ACAGGTTGCA AGTGAATTAA CCAGGTGTCT TCAGGGGAAG AGTTCCCAGT 
     1441 TATTACAAGG TGACTCTATT CAGGTTTCTG AAAATGAGAA CAATATAATG AACCCTAAAG 
     1501 GAGATAGCTC TATTTATATT GAAAATCAAG AGTTTCCATT TTGGAGAACC CAGCATTCTT 
     1561 GCGGGAATAC ATATCTGAGT GAGTCACAGA TTCAGAGTAG AGGTAAGCAA ATTGATGTGA 
     1621 AAAATAACCT GCAAATACAT GAAGACTTCA TGAAGAAATC ACCATTTCAT GAGCATATTA 
     1681 AAACTGACAC AGAACCAAAA CCCTGCAAAG GTAATGAATA TGGCAAAATC ATTAGTGATG 
     1741 GCTCCAACCA GAAATTACCC TTAGGAGAGA AACCCCATCC ATGTGGTGAG TGTGGAAGGG 
     1801 GCTTCAGTTA TAGCCCAAGG CTTCCCCTTC ATCCGAATGT TCACACAGGA GAAAAATGCT 
     1861 TCAGTCAAAG CTCACATCTG CGAACTCATC AGAGAATTCA CCCAGGAGAG AAACTCAATA 
     1921 GATGTCATGA ATCTGGTGAT TGCTTCAATA AGAGCTCTTT TCATTCTTAT CAATCTAATC 
     1981 ATACAGGAGA GAAGTCTTAT AGATGCGACA GTTGCGGCAA GGGATTCAGT AGCAGCACGG 
     2041 GTCTTATCAT TCATTACAGA ACTCATACTG GAGAGAAACC CTATAAATGC GAGGAATGTG 
     2101 GTAAATGCTT TAGTCAAAGT TCAAATTTTC AGTGCCATCA GAGAGTCCAC ACTGAAGAAA 
     2161 AACCATACAA ATGCGAAGAG TGTGGTAAGG GCTTCGGTTG GAGTGTTAAT CTCCGTGTTC 
     2221 ACCAGAGGGT CCACAGGGGT GAGAAGCCCT ATAAATGTGA GGAATGTGGT AAGGGCTTCA 
     2281 CTCAGGCTGC ACATTTTCAC ATCCATCAGA GAGTCCACAC TGGAGAGAAA CCCTACAAGT 
     2341 GTGATGTGTG TGGTAAGGGC TTCAGCCACA ATTCACCATT AATATGCCAT CGGAGAGTCC 
     2401 ACACAGGAGA GAAGCCATAC AAGTGTGAGG CGTGTGGGAA AGGCTTTACC CGTAATACAG 
     2461 ATCTGCATAT TCATTTCAGA GTTCACACGG GAGAGAAACC CTATAAATGT AAGGAGTGTG 
     2521 GTAAGGGCTT CAGTCAGGCT TCAAATCTTC AAGTCCATCA GAATGTCCAC ACTGGGGAGA 
     2581 AACGATTCAA GTGTGAAACG TGTGGGAAGG GCTTCAGTCA GTCCTCAAAG CTTCAAACCC 
     2641 ATCAGCGAGT CCACACTGGA GAGAAACCAT ATAGATGTGA TGTGTGTGGT AAGGACTTCA 
     2701 GTTATAGTTC AAATCTTAAA CTACACCAAG TAATTCACAC TGGAGAAAAA CCATATAAAT 
     2761 GTGAGGAATG TGGGAAGGGC TTCAGTTGGA GATCAAATCT TCATGCACAT CAAAGAGTTC 
     2821 ACTCAGGAGA AAAACCCTAT AAATGTGAGC AGTGTGATAA GAGCTTCAGT CAGGCCATAG 
     2881 ATTTTCGGGT ACATCAGAGA GTCCATACTG GAGAGAAGCC ATACAAATGT GGTGTCTGTG 
     2941 GTAAGGGCTT CAGTCAGTCC TCTGGTCTTC AATCCCATCA GAGAGTCCAC ACGGGGGAAA 
     3001 AGCCATACAA ATGTGATGTG TGTGGAAAGG GCTTTAGATA CAGTTCGCAG TTTATATACC 
     3061 ATCAGAGAGG CCACACTGGA GAAAAACCTT ACAAATGTGA AGAGTGTGGG AAAGGCTTTG 
     3121 GTAGGAGCTT GAATCTTCGC CATCATCAGA GGGTCCACAC GGGAGAGAAA CCCCATATAT 
     3181 GTGAGGAGTG TGGTAAGGCC TTCAGTCTCC CCTCAAATCT TCGAGTCCAC CTGGGTGTTC 
     3241 ACACCAGGGA AAAACTCTTT AAATGTGAAG AGTGTGGTAA AGGCTTCAGT CAGAGTGCAC 
     3301 GTCTTGAAGC CCATCAGAGA GTCCACACTG GAGAAAAACC ATACAAATGT GACATATGTG 
     3361 ATAAGGACTT CCGTCACCGT TCACGTCTTA CATATCATCA GAAAGTCCAT ACTGGTAAAA 
     3421 AGCTTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3481 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3541 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3601 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3661 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3721 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3781 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3841 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3901 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3961 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4021 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4081 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4141 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4201 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4261 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4321 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4381 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4441 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4501 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4561 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4621 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4681 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4741 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4801 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4861 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4921 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4981 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5041 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5101 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5161 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5221 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5281 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5341 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5401 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5461 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5521 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5581 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5641 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5701 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5761 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5821 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5881 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5941 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6001 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6061 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6121 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6181 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6241 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6301 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6361 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6421 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6481 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6541 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6601 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6661 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6721 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6781 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6841 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6901 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6961 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7021 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7081 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7141 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7201 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7261 AAATTTAACG CGAATTTT
//