LOCUS       RC207930                7356 bp    DNA     circular  SYN 
DEFINITION  BCAR3 (Myc-DDK-tagged)-Human breast cancer anti-estrogen resistance 3 (BCAR3)
ACCESSION   NM_003567
VERSION     NM_003567.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3513..3604 
                     /label="Myc-DDK"  
     misc_feature    3634..4219 
                     /label="PolyA signal"  
     misc_feature    3691..3710 
                     /label="Restriction Enzyme"  
     misc_feature    4495..5167 
                     /label="ColE1"  
     misc_feature    5597..6391 
                     /label="Kan/Neo"  
     misc_feature    6426..6728 
                     /label="SV40 ori"  
     misc_feature    6814..7291 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3495..3501 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3495 
                     /label="BCAR3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCAGGA AAATTTGCAA GCCTTCCCAG AAACATGCCG GTGAATCACC 
     1081 AGTTCCCCCT GGCCTCATCC ATGGACCTTC TGAGCAGCAG GTCCCCTCTC GCTGAGCATC 
     1141 GCCCAGATGC CTATCAAGAT GTGTCTATAC ATGGCACCCT TCCACGGAAG AAAAAAGGTC 
     1201 CTCCTCCCAT AAGGTCCTGT GATGACTTCA GTCACATGGG CACCCTCCCC CACTCCAAAT 
     1261 CCCCACGGCA GAACTCGCCT GTGACCCAGG ATGGCATCCA GGAGAGCCCA TGGCAGGACC 
     1321 GGCACGGCGA AACCTTCACC TTCAGGGATC CACATCTTCT GGACCCAACT GTGGAATATG 
     1381 TGAAGTTCTC CAAGGAGAGG CACATCATGG ACAGGACCCC CGAGAAACTG AAGAAGGAGC 
     1441 TGGAGGAGGA GCTGCTCCTG AGCAGCGAGG ACCTGCGCAG CCATGCCTGG TACCACGGCC 
     1501 GCATCCCCCG ACAGGTGTCT GAAAACCTTG TGCAGCGAGA TGGTGACTTC CTAGTTCGTG 
     1561 ACTCTCTGTC CAGCCCTGGG AACTTTGTCC TGACCTGTCA GTGGAAGAAC CTCGCTCAGC 
     1621 ACTTCAAAAT CAACCGGACA GTTCTGCGAC TCAGCGAGGC CTACAGCCGC GTGCAGTACC 
     1681 AGTTCGAGAT GGAGAGCTTC GACTCCATCC CCGGCCTGGT GCGCTGCTAC GTGGGCAACC 
     1741 GCCGGCCCAT CTCCCAGCAG AGTGGCGCCA TCATCTTCCA GCCCATCAAC AGGACGGTGC 
     1801 CTCTGCGGTG CCTGGAGGAG CATTATGGCA CCTCCCCAGG CCAGGCCCGG GAGGGCAGCC 
     1861 TCACCAAGGG AAGGCCGGAT GTGGCCAAGA GGCTGAGCCT CACCATGGGT GGCGTCCAGG 
     1921 CCCGAGAGCA GAATTTGCCC AGGGGAAACC TCCTCAGAAA CAAAGAAAAG AGTGGTAGCC 
     1981 AGCCCGCCTG CCTGGATCAC ATGCAGGACA GAAGAGCCTT GTCCCTCAAA GCCCACCAGT 
     2041 CAGAGAGCTA CCTGCCGATT GGCTGCAAGC TGCCACCTCA GTCCTCGGGT GTGGACACAA 
     2101 GCCCCTGCCC AAACTCACCT GTGTTCAGGA CGGGAAGCGA GCCTGCCCTG AGCCCAGCAG 
     2161 TGGTTCGGAG GGTCTCCTCA GACGCCAGGG CTGGGGAGGC GCTGAGGGGA TCAGACAGTC 
     2221 AACTGTGCCC TAAGCCCCCG CCTAAGCCCT GCAAGGTGCC GTTCCTCAAG GTTCCCTCGT 
     2281 CTCCCTCTGC CTGGCTCAAC TCAGAGGCCA ACTACTGTGA ACTGAACCCA GCGTTTGCCA 
     2341 CAGGCTGCGG CAGGGGAGCA AAGCTACCCT CATGTGCCCA GGGAAGCCAC ACAGAACTGC 
     2401 TCACAGCCAA GCAGAATGAG GCGCCAGGTC CCCGGAACTC TGGCGTCAAC TACTTGATCC 
     2461 TTGATGATGA TGACAGGGAA AGACCTTGGG AACCTGCGGC AGCTCAGATG GAGAAGGGGC 
     2521 AGTGGGACAA GGGCGAGTTT GTGACGCCCC TCCTGGAGAC TGTCTCCTCC TTCAGGCCCA 
     2581 ACGAGTTTGA GTCAAAGTTC CTTCCCCCTG AGAATAAGCC CCTGGAAACA GCAATGTTGA 
     2641 AACGTGCAAA AGAACTGTTC ACCAACAACG ACCCCAAGGT CATCGCCCAG CACGTACTGA 
     2701 GCATGGACTG CAGGGTTGCT AGGATACTTG GAGTCTCTGA AGAGATGAGG AGGAACATGG 
     2761 GGGTGAGCTC AGGCCTGGAA CTCATTACCT TGCCTCACGG ACACCAGCTG CGCCTGGACA 
     2821 TAATTGAAAG ACACAACACA ATGGCCATCG GCATTGCAGT GGACATTCTG GGATGCACGG 
     2881 GCACTTTGGA GGACCGAGCG GCCACTCTGA GTAAGATCAT CCAGGTGGCG GTGGAACTGA 
     2941 AGGATTCCAT GGGGGACCTC TATTCCTTCT CAGCTCTCAT GAAAGCCCTG GAAATGCCAC 
     3001 AGATCACAAG GTTAGAAAAG ACGTGGACTG CTCTGCGGCA CCAGTACACC CAAACTGCCA 
     3061 TTCTCTATGA GAAACAGCTG AAGCCCTTCA GCAAACTCCT GCATGAAGGC AGAGAGTCCA 
     3121 CATGTGTTCC CCCAAACAAT GTATCAGTCC CACTGCTGAT GCCGCTTGTG ACGTTAATGG 
     3181 AGCGCCAGGC TGTGACTTTT GAAGGAACCG ACATGTGGGA AAAAAACGAC CAGAGCTGTG 
     3241 AAATCATGCT GAACCATTTG GCAACAGCGC GATTCATGGC CGAGGCTGCA GACAGCTACC 
     3301 GGATGAATGC TGAGAGGATC CTGGCAGGTT TTCAACCAGA TGAAGAAATG AATGAAATCT 
     3361 GCAAGACTGA ATTTCAAATG CGATTGCTAT GGGGCAGCAA AGGTGCACAA GTCAATCAGA 
     3421 CAGAGAGATA TGAGAAATTC AACCAGATTT TAACTGCCCT CTCGCGTAAA TTGGAACCTC 
     3481 CTCCTGTAAA GCAGGCAGAG CTTACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3541 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3601 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3661 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3721 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3781 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3841 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3901 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3961 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4021 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4081 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4141 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4201 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4261 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4321 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4381 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4441 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4501 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4561 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4621 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4681 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4741 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4801 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4861 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4921 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4981 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5041 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5101 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5161 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5221 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5281 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5341 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5401 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5461 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5521 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5581 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5641 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5701 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5761 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5821 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5881 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5941 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6001 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6061 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6121 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6181 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6241 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6301 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6361 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6421 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6481 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6541 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6601 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6661 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6721 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6781 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6841 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6901 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6961 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7021 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7081 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7141 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7201 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7261 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7321 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//