LOCUS       RC207920L1                9399 bp    DNA     circular  SYN 
DEFINITION  HLTF (Myc-DDK-tagged)-Human helicase-like transcription factor (HLTF), transcript variant 1
ACCESSION   NM_003071
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7690..7870 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8598..9257 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8029..8597 
                     /label="ColE1"  
     misc_feature    6565..6585 
                     /label="LR50 primer"  
     misc_feature    6362..6433 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6574..7165 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6344..6350 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6344 
                     /label="HLTF"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTCCTGGATG TTCAAGAGGG ATCCAGTTTG 
     3361 GAAGTACTTG CAGACTGTCC AGTATGGAGT TCATGGAAAT TTTCCACGCC TCTCATATCC 
     3421 AACTTTCTTT CCACGTTTTG AATTCCAAGA TGTTATCCCT CCAGATGACT TTCTAACTAG 
     3481 TGATGAAGAA GTAGATTCCG TTTTATTTGG AAGTTTGAGA GGTCATGTGG TTGGACTACG 
     3541 CTATTACACG GGAGTAGTTA ATAATAATGA AATGGTTGCA TTACAACGAG ATCCTAATAA 
     3601 CCCTTATGAT AAGAATGCAA TTAAAGTAAA CAATGTGAAT GGAAATCAAG TTGGCCATTT 
     3661 AAAGAAAGAG CTTGCAGGTG CTTTGGCCTA TATCATGGAC AACAAATTGG CACAAATTGA 
     3721 AGGGGTAGTT CCTTTTGGTG CAAACAATGC TTTTACCATG CCTCTGCATA TGACTTTTTG 
     3781 GGGAAAAGAA GAAAATAGAA AAGCGGTTTC AGATCAGTTG AAGAAACATG GATTTAAATT 
     3841 GGGTCCTGCA CCAAAAACTT TAGGATTCAA TTTGGAAAGT GGTTGGGGCT CTGGAAGAGC 
     3901 TGGACCAAGC TATAGTATGC CAGTGCATGC TGCAGTACAG ATGACAACTG AACAGCTTAA 
     3961 AACAGAATTT GACAAATTGT TTGAAGATTT AAAAGAAGAT GATAAAACCC ATGAAATGGA 
     4021 ACCAGCTGAG GCTATTGAAA CACCACTGCT TCCACATCAA AAACAAGCTC TAGCTTGGAT 
     4081 GGTGTCACGG GAAAATAGCA AAGAACTTCC ACCATTCTGG GAACAGCGAA ATGACTTATA 
     4141 CTATAACACA ATAACAAATT TTTCTGAGAA GGACCGACCA GAAAATGTCC ATGGAGGAAT 
     4201 TTTAGCTGAT GATATGGGTT TGGGTAAAAC TCTTACAGCC ATTGCAGTAA TCCTTACCAA 
     4261 CTTCCATGAT GGCAGACCTC TTCCTATTGA AAGAGTTAAA AAGAATCTAC TGAAGAAGGA 
     4321 ATATAATGTT AACGATGACT CTATGAAACT TGGAGGAAAC AATACCAGTG AAAAGGCAGA 
     4381 TGGACTAAGC AAAGACGCAT CTAGATGTAG TGAACAACCC AGTATTTCAG ATATCAAGGA 
     4441 GAAGAGTAAG TTTCGCATGT CAGAATTGTC TAGCTCCCGC CCCAAAAGAA GAAAAACTGC 
     4501 TGTCCAGTAC ATAGAAAGCA GTGATTCAGA GGAAATTGAA ACAAGTGAAT TGCCGCAGAA 
     4561 AATGAAAGGC AAACTGAAAA ATGTACAGTC TGAAACTAAA GGCAGGGCGA AAGGATCTTC 
     4621 TAAGGTTATA GAAGATGTGG CATTTGCATG TGCATTAACT TCATCTGTTC CTACAACAAA 
     4681 AAAGAAAATG TTGAAAAAGG GAGCTTGTGC AGTGGAGGGG TCAAAGAAAA CTGATGTTGA 
     4741 GGAGAGACCA AGAACAACAC TGATCATCTG TCCGCTTTCT GTGTTAAGCA ACTGGATTGA 
     4801 CCAGTTTGGA CAACATATAA AATCAGATGT ACACTTGAAT TTTTATGTTT ATTATGGTCC 
     4861 TGATCGTATT AGAGAACCGG CCTTACTTTC AAAACAGGAT ATTGTTTTGA CTACGTATAA 
     4921 TATTTTAACT CATGACTATG GAACTAAAGG AGATAGTCCA TTACATAGCA TAAGGTGGCT 
     4981 AAGAGTGATC CTGGATGAAG GACATGCCAT ACGAAATCCA AATGCTCAGC AGACAAAAGC 
     5041 TGTACTTGAC TTAGAATCAG AAAGAAGATG GGTTTTGACA GGTACTCCAA TCCAGAATTC 
     5101 TTTAAAGGAC TTGTGGTCTC TTCTTTCCTT TTTAAAACTT AAACCATTTA TTGATAGAGA 
     5161 ATGGTGGCAT AGAACAATAC AGCGTCCTGT CACAATGGGA GATGAAGGAG GACTTAGGCG 
     5221 TTTACAGTCC CTAATTAAAA ATATTACACT TAGAAGAACA AAGACAAGCA AAATTAAAGG 
     5281 AAAACCTGTT TTGGAGTTAC CAGAACGTAA AGTATTTATT CAGCACATTA CACTTTCAGA 
     5341 TGAAGAGAGA AAGATTTATC AGTCTGTGAA AAATGAAGGC AGAGCCACTA TTGGAAGGTA 
     5401 TTTTAATGAA GGGACTGTCC TGGCACATTA TGCAGATGTC CTGGGTCTTT TGCTTAGACT 
     5461 GCGGCAAATT TGTTGCCATA CTTACCTTCT TACAAATGCA GTGTCTTCCA ATGGCCCCTC 
     5521 AGGAAATGAT ACACCTGAAG AACTGAGAAA GAAGTTAATA AGGAAGATGA AGTTAATTCT 
     5581 GAGCTCAGGT TCAGATGAGG AATGTGCAAT TTGCCTGGAT TCTTTAACAG TTCCTGTGAT 
     5641 AACACATTGT GCACATGTAT TTTGTAAACC CTGTATTTGC CAAGTCATTC AGAATGAGCA 
     5701 GCCACATGCT AAATGCCCTT TATGCAGAAA TGATATACAT GAAGATAATT TATTAGAATG 
     5761 TCCTCCAGAA GAATTAGCAC GTGACAGTGA GAAAAAGTCT GATATGGAAT GGACATCCAG 
     5821 TTCAAAGATT AATGCGCTAA TGCACGCATT GACTGACTTA AGAAAGAAGA ATCCCAACAT 
     5881 AAAAAGTTTG GTTGTTTCTC AGTTTACAAC ATTCCTGTCT TTAATAGAAA TACCACTTAA 
     5941 AGCCTCTGGA TTTGTGTTTA CTCGTTTGGA TGGTTCCATG GCCCAAAAGA AAAGAGTTGA 
     6001 ATCAATTCAG TGTTTTCAAA ACACTGAAGC AGGATCTCCA ACTATAATGC TTCTGTCCTT 
     6061 AAAAGCAGGT GGAGTTGGTT TGAATCTGTC TGCAGCTTCT CGAGTGTTTT TAATGGATCC 
     6121 AGCCTGGAAT CCTGCTGCTG AAGATCAGTG CTTTGACAGA TGCCATAGAC TTGGTCAGAA 
     6181 GCAAGAAGTT ATCATCACAA AATTCATTGT AAAGGACTCT GTTGAAGAAA ATATGCTGAA 
     6241 AATACAAAAC AAAAAGAGAG AACTTGCAGC AGGAGCCTTT GGAACTAAAA AACCAAATGC 
     6301 TGACGAAATG AAACAAGCCA AAATTAATGA AATCAGAACA TTAATTGACT TAACGCGTAC 
     6361 GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA 
     6421 TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCTGTACA AGTAGGATTC 
     6481 GTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATACA TGTTTAAGGG 
     6541 TTCCGGTTCC ACTAGGTACA ATTCGATATC AAGCTTATCG ATAATCAACC TCTGGATTAC 
     6601 AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA 
     6661 TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC 
     6721 TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA 
     6781 CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC 
     6841 ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC 
     6901 ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC 
     6961 GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG 
     7021 ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT 
     7081 TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG 
     7141 AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CATCGATACC GTCGACCTCG ATCGAGACCT 
     7201 AGAAAAACAT GGAGCAATCA CAAGTAGCAA TACAGCAGCT ACCAATGCTG ATTGTGCCTG 
     7261 GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC ACACCTCAGG TACCTTTAAG 
     7321 ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT 
     7381 GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATATCCTT GATCTGTGGA TCTACCACAC 
     7441 ACAAGGCTAC TTCCCTGATT GGCAGAACTA CACACCAGGG CCAGGGATCA GATATCCACT 
     7501 GACCTTTGGA TGGTGCTACA AGCTAGTACC AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA 
     7561 TGAAGGAGAG AACACCCGCT TGTTACACCC TGTGAGCCTG CATGGGATGG ATGACCCGGA 
     7621 GAGAGAAGTA TTAGAGTGGA GGTTTGACAG CCGCCTAGCA TTTCATCACA TGGCCCGAGA 
     7681 GCTGCATCCG GACTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC 
     7741 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT 
     7801 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC 
     7861 AGTGTGGAAA ATCTCTAGCA GCATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     7921 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     7981 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     8041 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     8101 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     8161 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     8221 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     8281 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     8341 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     8401 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     8461 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     8521 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     8581 CTCACGTTAA GGGATTTTGG TCATGATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT 
     8641 TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AAACGGCATG ATGAACCTGA 
     8701 ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG 
     8761 GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG 
     8821 GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT 
     8881 TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG 
     8941 TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG 
     9001 TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGGAA CTCCGGATGA 
     9061 GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC 
     9121 TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA 
     9181 GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG 
     9241 GTATATCCAG TGATTTTTTT CTCCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     9301 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     9361 GGGGTCCCGC GCACATTTCC CCGAAAAGTG CCACCTGAC
//