LOCUS       RC207920                7905 bp    DNA     circular  SYN 
DEFINITION  HLTF (Myc-DDK-tagged)-Human helicase-like transcription factor (HLTF), transcript variant 1
ACCESSION   NM_003071
VERSION     NM_003071.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4062..4153 
                     /label="Myc-DDK"  
     misc_feature    4183..4768 
                     /label="PolyA signal"  
     misc_feature    4240..4259 
                     /label="Restriction Enzyme"  
     misc_feature    5044..5716 
                     /label="ColE1"  
     misc_feature    6146..6940 
                     /label="Kan/Neo"  
     misc_feature    6975..7277 
                     /label="SV40 ori"  
     misc_feature    7363..7840 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4044..4050 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4044 
                     /label="HLTF"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTGGATG TTCAAGAGGG ATCCAGTTTG GAAGTACTTG CAGACTGTCC 
     1081 AGTATGGAGT TCATGGAAAT TTTCCACGCC TCTCATATCC AACTTTCTTT CCACGTTTTG 
     1141 AATTCCAAGA TGTTATCCCT CCAGATGACT TTCTAACTAG TGATGAAGAA GTAGATTCCG 
     1201 TTTTATTTGG AAGTTTGAGA GGTCATGTGG TTGGACTACG CTATTACACG GGAGTAGTTA 
     1261 ATAATAATGA AATGGTTGCA TTACAACGAG ATCCTAATAA CCCTTATGAT AAGAATGCAA 
     1321 TTAAAGTAAA CAATGTGAAT GGAAATCAAG TTGGCCATTT AAAGAAAGAG CTTGCAGGTG 
     1381 CTTTGGCCTA TATCATGGAC AACAAATTGG CACAAATTGA AGGGGTAGTT CCTTTTGGTG 
     1441 CAAACAATGC TTTTACCATG CCTCTGCATA TGACTTTTTG GGGAAAAGAA GAAAATAGAA 
     1501 AAGCGGTTTC AGATCAGTTG AAGAAACATG GATTTAAATT GGGTCCTGCA CCAAAAACTT 
     1561 TAGGATTCAA TTTGGAAAGT GGTTGGGGCT CTGGAAGAGC TGGACCAAGC TATAGTATGC 
     1621 CAGTGCATGC TGCAGTACAG ATGACAACTG AACAGCTTAA AACAGAATTT GACAAATTGT 
     1681 TTGAAGATTT AAAAGAAGAT GATAAAACCC ATGAAATGGA ACCAGCTGAG GCTATTGAAA 
     1741 CACCACTGCT TCCACATCAA AAACAAGCTC TAGCTTGGAT GGTGTCACGG GAAAATAGCA 
     1801 AAGAACTTCC ACCATTCTGG GAACAGCGAA ATGACTTATA CTATAACACA ATAACAAATT 
     1861 TTTCTGAGAA GGACCGACCA GAAAATGTCC ATGGAGGAAT TTTAGCTGAT GATATGGGTT 
     1921 TGGGTAAAAC TCTTACAGCC ATTGCAGTAA TCCTTACCAA CTTCCATGAT GGCAGACCTC 
     1981 TTCCTATTGA AAGAGTTAAA AAGAATCTAC TGAAGAAGGA ATATAATGTT AACGATGACT 
     2041 CTATGAAACT TGGAGGAAAC AATACCAGTG AAAAGGCAGA TGGACTAAGC AAAGACGCAT 
     2101 CTAGATGTAG TGAACAACCC AGTATTTCAG ATATCAAGGA GAAGAGTAAG TTTCGCATGT 
     2161 CAGAATTGTC TAGCTCCCGC CCCAAAAGAA GAAAAACTGC TGTCCAGTAC ATAGAAAGCA 
     2221 GTGATTCAGA GGAAATTGAA ACAAGTGAAT TGCCGCAGAA AATGAAAGGC AAACTGAAAA 
     2281 ATGTACAGTC TGAAACTAAA GGCAGGGCGA AAGGATCTTC TAAGGTTATA GAAGATGTGG 
     2341 CATTTGCATG TGCATTAACT TCATCTGTTC CTACAACAAA AAAGAAAATG TTGAAAAAGG 
     2401 GAGCTTGTGC AGTGGAGGGG TCAAAGAAAA CTGATGTTGA GGAGAGACCA AGAACAACAC 
     2461 TGATCATCTG TCCGCTTTCT GTGTTAAGCA ACTGGATTGA CCAGTTTGGA CAACATATAA 
     2521 AATCAGATGT ACACTTGAAT TTTTATGTTT ATTATGGTCC TGATCGTATT AGAGAACCGG 
     2581 CCTTACTTTC AAAACAGGAT ATTGTTTTGA CTACGTATAA TATTTTAACT CATGACTATG 
     2641 GAACTAAAGG AGATAGTCCA TTACATAGCA TAAGGTGGCT AAGAGTGATC CTGGATGAAG 
     2701 GACATGCCAT ACGAAATCCA AATGCTCAGC AGACAAAAGC TGTACTTGAC TTAGAATCAG 
     2761 AAAGAAGATG GGTTTTGACA GGTACTCCAA TCCAGAATTC TTTAAAGGAC TTGTGGTCTC 
     2821 TTCTTTCCTT TTTAAAACTT AAACCATTTA TTGATAGAGA ATGGTGGCAT AGAACAATAC 
     2881 AGCGTCCTGT CACAATGGGA GATGAAGGAG GACTTAGGCG TTTACAGTCC CTAATTAAAA 
     2941 ATATTACACT TAGAAGAACA AAGACAAGCA AAATTAAAGG AAAACCTGTT TTGGAGTTAC 
     3001 CAGAACGTAA AGTATTTATT CAGCACATTA CACTTTCAGA TGAAGAGAGA AAGATTTATC 
     3061 AGTCTGTGAA AAATGAAGGC AGAGCCACTA TTGGAAGGTA TTTTAATGAA GGGACTGTCC 
     3121 TGGCACATTA TGCAGATGTC CTGGGTCTTT TGCTTAGACT GCGGCAAATT TGTTGCCATA 
     3181 CTTACCTTCT TACAAATGCA GTGTCTTCCA ATGGCCCCTC AGGAAATGAT ACACCTGAAG 
     3241 AACTGAGAAA GAAGTTAATA AGGAAGATGA AGTTAATTCT GAGCTCAGGT TCAGATGAGG 
     3301 AATGTGCAAT TTGCCTGGAT TCTTTAACAG TTCCTGTGAT AACACATTGT GCACATGTAT 
     3361 TTTGTAAACC CTGTATTTGC CAAGTCATTC AGAATGAGCA GCCACATGCT AAATGCCCTT 
     3421 TATGCAGAAA TGATATACAT GAAGATAATT TATTAGAATG TCCTCCAGAA GAATTAGCAC 
     3481 GTGACAGTGA GAAAAAGTCT GATATGGAAT GGACATCCAG TTCAAAGATT AATGCGCTAA 
     3541 TGCACGCATT GACTGACTTA AGAAAGAAGA ATCCCAACAT AAAAAGTTTG GTTGTTTCTC 
     3601 AGTTTACAAC ATTCCTGTCT TTAATAGAAA TACCACTTAA AGCCTCTGGA TTTGTGTTTA 
     3661 CTCGTTTGGA TGGTTCCATG GCCCAAAAGA AAAGAGTTGA ATCAATTCAG TGTTTTCAAA 
     3721 ACACTGAAGC AGGATCTCCA ACTATAATGC TTCTGTCCTT AAAAGCAGGT GGAGTTGGTT 
     3781 TGAATCTGTC TGCAGCTTCT CGAGTGTTTT TAATGGATCC AGCCTGGAAT CCTGCTGCTG 
     3841 AAGATCAGTG CTTTGACAGA TGCCATAGAC TTGGTCAGAA GCAAGAAGTT ATCATCACAA 
     3901 AATTCATTGT AAAGGACTCT GTTGAAGAAA ATATGCTGAA AATACAAAAC AAAAAGAGAG 
     3961 AACTTGCAGC AGGAGCCTTT GGAACTAAAA AACCAAATGC TGACGAAATG AAACAAGCCA 
     4021 AAATTAATGA AATCAGAACA TTAATTGACT TAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4081 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4141 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4201 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4261 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4321 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4381 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4441 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4501 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4561 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4621 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4681 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4741 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4801 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4861 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4921 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4981 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5041 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5101 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5161 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5221 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5281 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5341 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5401 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5461 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5521 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5581 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5641 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5701 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5761 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5821 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5881 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5941 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6001 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6061 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6121 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6181 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6241 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6301 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6361 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6421 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6481 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6541 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6601 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6661 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6721 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6781 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6841 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6901 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6961 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7021 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7081 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7141 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7201 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7261 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7321 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7381 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7441 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7501 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7561 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7621 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7681 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7741 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7801 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7861 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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