LOCUS       RC207905                9303 bp    DNA     circular  SYN 
DEFINITION  A2M (Myc-DDK-tagged)-Human alpha-2-macroglobulin (A2M)
ACCESSION   NM_000014
VERSION     NM_000014.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5460..5551 
                     /label="Myc-DDK"  
     misc_feature    5581..6166 
                     /label="PolyA signal"  
     misc_feature    5638..5657 
                     /label="Restriction Enzyme"  
     misc_feature    6442..7114 
                     /label="ColE1"  
     misc_feature    7544..8338 
                     /label="Kan/Neo"  
     misc_feature    8373..8675 
                     /label="SV40 ori"  
     misc_feature    8761..9238 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5442..5448 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5442 
                     /label="A2M"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGAAGAAC AAACTCCTTC ATCCAAGTCT GGTTCTTCTC CTCTTGGTCC 
     1081 TCCTGCCCAC AGACGCCTCA GTCTCTGGAA AACCGCAGTA TATGGTTCTG GTCCCCTCCC 
     1141 TGCTCCACAC TGAGACCACT GAGAAGGGCT GTGTCCTTCT GAGCTACCTG AATGAGACAG 
     1201 TGACTGTAAG TGCTTCCTTG GAGTCTGTCA GGGGAAACAG GAGCCTCTTC ACTGACCTGG 
     1261 AGGCGGAGAA TGACGTACTC CACTGTGTCG CCTTCGCTGT CCCAAAGTCT TCATCCAATG 
     1321 AGGAGGTAAT GTTCCTCACT GTCCAAGTGA AAGGACCAAC CCAAGAATTT AAGAAGCGGA 
     1381 CCACAGTGAT GGTTAAGAAC GAGGACAGTC TGGTCTTTGT CCAGACAGAC AAATCAATCT 
     1441 ACAAACCAGG GCAGACAGTG AAATTTCGTG TTGTCTCCAT GGATGAAAAC TTTCACCCCC 
     1501 TGAATGAGTT GATTCCACTA GTATACATTC AGGATCCCAA AGGAAATCGC ATCGCACAAT 
     1561 GGCAGAGTTT CCAGTTAGAG GGTGGCCTCA AGCAATTTTC TTTTCCCCTC TCATCAGAGC 
     1621 CCTTCCAGGG CTCCTACAAG GTGGTGGTAC AGAAGAAATC AGGTGGAAGG ACAGAGCACC 
     1681 CTTTCACCGT GGAGGAATTT GTTCTTCCCA AGTTTGAAGT ACAAGTAACA GTGCCAAAGA 
     1741 TAATCACCAT CTTGGAAGAA GAGATGAATG TATCAGTGTG TGGCCTATAC ACATATGGGA 
     1801 AGCCTGTCCC TGGACATGTG ACTGTGAGCA TTTGCAGAAA GTATAGTGAC GCTTCCGACT 
     1861 GCCACGGTGA AGATTCACAG GCTTTCTGTG AGAAATTCAG TGGACAGCTA AACAGCCATG 
     1921 GCTGCTTCTA TCAGCAAGTA AAAACCAAGG TCTTCCAGCT GAAGAGGAAG GAGTATGAAA 
     1981 TGAAACTTCA CACTGAGGCC CAGATCCAAG AAGAAGGAAC AGTGGTGGAA TTGACTGGAA 
     2041 GGCAGTCCAG TGAAATCACA AGAACCATAA CCAAACTCTC ATTTGTGAAA GTGGACTCAC 
     2101 ACTTTCGACA GGGAATTCCC TTCTTTGGGC AGGTGCGCCT AGTAGATGGG AAAGGCGTCC 
     2161 CTATACCAAA TAAAGTCATA TTCATCAGAG GAAATGAAGC AAACTATTAC TCCAATGCTA 
     2221 CCACGGATGA GCATGGCCTT GTACAGTTCT CTATCAACAC CACCAATGTT ATGGGTACCT 
     2281 CTCTTACTGT TAGGGTCAAT TACAAGGATC GTAGTCCCTG TTACGGCTAC CAGTGGGTGT 
     2341 CAGAAGAACA CGAAGAGGCA CATCACACTG CTTATCTTGT GTTCTCCCCA AGCAAGAGCT 
     2401 TTGTCCACCT TGAGCCCATG TCTCATGAAC TACCCTGTGG CCATACTCAG ACAGTCCAGG 
     2461 CACATTATAT TCTGAATGGA GGCACCCTGC TGGGGCTGAA GAAGCTCTCC TTCTATTATC 
     2521 TGATAATGGC AAAGGGAGGC ATTGTCCGAA CTGGGACTCA TGGACTGCTT GTGAAGCAGG 
     2581 AAGACATGAA GGGCCATTTT TCCATCTCAA TCCCTGTGAA GTCAGACATT GCTCCTGTCG 
     2641 CTCGGTTGCT CATCTATGCT GTTTTACCTA CCGGGGACGT GATTGGGGAT TCTGCAAAAT 
     2701 ATGATGTTGA AAATTGTCTG GCCAACAAGG TGGATTTGAG CTTCAGCCCA TCACAAAGTC 
     2761 TCCCAGCCTC ACACGCCCAC CTGCGAGTCA CAGCGGCTCC TCAGTCCGTC TGCGCCCTCC 
     2821 GTGCTGTGGA CCAAAGCGTG CTGCTCATGA AGCCTGATGC TGAGCTCTCG GCGTCCTCGG 
     2881 TTTACAACCT GCTACCAGAA AAGGACCTCA CTGGCTTCCC TGGGCCTTTG AATGACCAGG 
     2941 ACGATGAAGA CTGCATCAAT CGTCATAATG TCTATATTAA TGGAATCACA TATACTCCAG 
     3001 TATCAAGTAC AAATGAAAAG GATATGTACA GCTTCCTAGA GGACATGGGC TTAAAGGCAT 
     3061 TCACCAACTC AAAGATTCGT AAACCCAAAA TGTGTCCACA GCTTCAACAG TATGAAATGC 
     3121 ATGGACCTGA AGGTCTACGT GTAGGTTTTT ATGAGTCAGA TGTAATGGGA AGAGGCCATG 
     3181 CACGCCTGGT GCATGTTGAA GAGCCTCACA CGGAGACCGT ACGAAAGTAC TTCCCTGAGA 
     3241 CATGGATCTG GGATTTGGTG GTGGTAAACT CAGCAGGTGT GGCTGAGGTA GGAGTAACAG 
     3301 TCCCTGACAC CATCACCGAG TGGAAGGCAG GGGCCTTCTG CCTGTCTGAA GATGCTGGAC 
     3361 TTGGTATCTC TTCCACTGCC TCTCTCCGAG CCTTCCAGCC CTTCTTTGTG GAGCTCACAA 
     3421 TGCCTTACTC TGTGATTCGT GGAGAGGCCT TCACACTCAA GGCCACGGTC CTAAACTACC 
     3481 TTCCCAAATG CATCCGGGTC AGTGTGCAGC TGGAAGCCTC TCCCGCCTTC CTAGCTGTCC 
     3541 CAGTGGAGAA GGAACAAGCG CCTCACTGCA TCTGTGCAAA CGGGCGGCAA ACTGTGTCCT 
     3601 GGGCAGTAAC CCCAAAGTCA TTAGGAAATG TGAATTTCAC TGTGAGCGCA GAGGCACTAG 
     3661 AGTCTCAAGA GCTGTGTGGG ACTGAGGTGC CTTCAGTTCC TGAACACGGA AGGAAAGACA 
     3721 CAGTCATCAA GCCTCTGTTG GTTGAACCTG AAGGACTAGA GAAGGAAACA ACATTCAACT 
     3781 CCCTACTTTG TCCATCAGGT GGTGAGGTTT CTGAAGAATT ATCCCTGAAA CTGCCACCAA 
     3841 ATGTGGTAGA AGAATCTGCC CGAGCTTCTG TCTCAGTTTT GGGAGACATA TTAGGCTCTG 
     3901 CCATGCAAAA CACACAAAAT CTTCTCCAGA TGCCCTATGG CTGTGGAGAG CAGAATATGG 
     3961 TCCTCTTTGC TCCTAACATC TATGTACTGG ATTATCTAAA TGAAACACAG CAGCTTACTC 
     4021 CAGAGGTCAA GTCCAAGGCC ATTGGCTATC TCAACACTGG TTACCAGAGA CAGTTGAACT 
     4081 ACAAACACTA TGATGGCTCC TACAGCACCT TTGGGGAGCG ATATGGCAGG AACCAGGGCA 
     4141 ACACCTGGCT CACAGCCTTT GTTCTGAAGA CTTTTGCCCA AGCTCGAGCC TACATCTTCA 
     4201 TCGATGAAGC ACACATTACC CAAGCCCTCA TATGGCTCTC CCAGAGGCAG AAGGACAATG 
     4261 GCTGTTTCAG GAGCTCTGGG TCACTGCTCA ACAATGCCAT AAAGGGAGGA GTAGAAGATG 
     4321 AAGTGACCCT CTCCGCCTAT ATCACCATCG CCCTTCTGGA GATTCCTCTC ACAGTCACTC 
     4381 ACCCTGTTGT CCGCAATGCC CTGTTTTGCC TGGAGTCAGC CTGGAAGACA GCACAAGAAG 
     4441 GGGACCATGG CAGCCATGTA TATACCAAAG CACTGCTGGC CTATGCTTTT GCCCTGGCAG 
     4501 GTAACCAGGA CAAGAGGAAG GAAGTACTCA AGTCACTTAA TGAGGAAGCT GTGAAGAAAG 
     4561 ACAACTCTGT CCATTGGGAG CGCCCTCAGA AACCCAAGGC ACCAGTGGGG CATTTTTACG 
     4621 AACCCCAGGC TCCCTCTGCT GAGGTGGAGA TGACATCCTA TGTGCTCCTC GCTTATCTCA 
     4681 CGGCCCAGCC AGCCCCAACC TCGGAGGACC TGACCTCTGC AACCAACATC GTGAAGTGGA 
     4741 TCACGAAGCA GCAGAATGCC CAGGGCGGTT TCTCCTCCAC CCAGGACACA GTGGTGGCTC 
     4801 TCCATGCTCT GTCCAAATAT GGAGCAGCCA CATTTACCAG GACTGGGAAG GCTGCACAGG 
     4861 TGACTATCCA GTCTTCAGGG ACATTTTCCA GCAAATTCCA AGTGGACAAC AACAACCGCC 
     4921 TGTTACTGCA GCAGGTCTCA TTGCCAGAGC TGCCTGGGGA ATACAGCATG AAAGTGACAG 
     4981 GAGAAGGATG TGTCTACCTC CAGACATCCT TGAAATACAA TATTCTCCCA GAAAAGGAAG 
     5041 AGTTCCCCTT TGCTTTAGGA GTGCAGACTC TGCCTCAAAC TTGTGATGAA CCCAAAGCCC 
     5101 ACACCAGCTT CCAAATCTCC CTAAGTGTCA GTTACACAGG GAGCCGCTCT GCCTCCAACA 
     5161 TGGCGATCGT TGATGTGAAG ATGGTCTCTG GCTTCATTCC CCTGAAGCCA ACAGTGAAAA 
     5221 TGCTTGAAAG ATCTAACCAT GTGAGCCGGA CAGAAGTCAG CAGCAACCAT GTCTTGATTT 
     5281 ACCTTGATAA GGTGTCAAAT CAGACACTGA GCTTGTTCTT CACGGTTCTG CAAGATGTCC 
     5341 CAGTAAGAGA TCTGAAACCA GCCATAGTGA AAGTCTATGA TTACTACGAG ACGGATGAGT 
     5401 TTGCAATTGC TGAGTACAAT GCTCCTTGCA GCAAAGATCT TGGAAATGCT ACGCGTACGC 
     5461 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5521 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5581 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5641 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5701 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5761 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5821 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5881 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5941 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     6001 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     6061 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     6121 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     6181 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     6241 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6301 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6361 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6421 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6481 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6541 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6601 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6661 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6721 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6781 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6841 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6901 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6961 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     7021 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     7081 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     7141 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     7201 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     7261 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7321 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7381 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7441 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7501 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7561 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7621 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7681 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7741 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7801 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7861 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7921 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7981 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     8041 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     8101 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     8161 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     8221 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     8281 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8341 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8401 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8461 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8521 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8581 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8641 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8701 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8761 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8821 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8881 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8941 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     9001 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     9061 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     9121 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     9181 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     9241 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9301 TTT
//