LOCUS       RC207899                7281 bp    DNA     circular  SYN 
DEFINITION  CHSY1 (Myc-DDK-tagged)-Human chondroitin sulfate synthase 1 (CHSY1)
ACCESSION   NM_014918
VERSION     NM_014918.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3436..3527 
                     /label="Myc-DDK"  
     misc_feature    3557..4142 
                     /label="PolyA signal"  
     misc_feature    3614..3633 
                     /label="Restriction Enzyme"  
     misc_feature    4418..5090 
                     /label="ColE1"  
     misc_feature    5520..6314 
                     /label="Kan/Neo"  
     misc_feature    6349..6651 
                     /label="SV40 ori"  
     misc_feature    6737..7214 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3426..3434 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3426 
                     /label="CHSY1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGCGCGC GGCCGGCGCG CCTGGCTCAG CGTGCTGCTC GGGCTCGTCC 
     1081 TGGGCTTCGT GCTGGCCTCG CGGCTCGTCC TGCCCCGGGC TTCCGAGCTG AAGCGAGCGG 
     1141 GCCCACGGCG CCGCGCCAGC CCCGAGGGCT GCCGGTCCGG GCAGGCGGCG GCTTCCCAGG 
     1201 CCGGCGGGGC GCGCGGCGAT GCGCGCGGGG CGCAGCTCTG GCCGCCCGGC TCGGACCCAG 
     1261 ATGGCGGCCC GCGCGACAGG AACTTTCTCT TCGTGGGAGT CATGACCGCC CAGAAATACC 
     1321 TGCAGACTCG GGCCGTGGCC GCCTACAGAA CATGGTCCAA GACAATTCCT GGGAAAGTTC 
     1381 AGTTCTTCTC AAGTGAGGGT TCTGACACAT CTGTACCAAT TCCAGTAGTG CCACTACGGG 
     1441 GTGTGGACGA CTCCTACCCG CCCCAGAAGA AGTCCTTCAT GATGCTCAAG TACATGCACG 
     1501 ACCACTACTT GGACAAGTAT GAATGGTTTA TGAGAGCAGA TGATGACGTG TACATCAAAG 
     1561 GAGACCGTCT GGAGAACTTC CTGAGGAGTT TGAACAGCAG CGAGCCCCTC TTTCTTGGGC 
     1621 AGACAGGCCT GGGCACCACG GAAGAAATGG GAAAACTGGC CCTGGAGCCT GGTGAGAACT 
     1681 TCTGCATGGG GGGGCCTGGC GTGATCATGA GCCGGGAGGT GCTTCGGAGA ATGGTGCCGC 
     1741 ACATTGGCAA GTGTCTCCGG GAGATGTACA CCACCCATGA GGACGTGGAG GTGGGAAGGT 
     1801 GTGTCCGGAG GTTTGCAGGG GTGCAGTGTG TCTGGTCTTA TGAGATGCAG CAGCTTTTTT 
     1861 ATGAGAATTA CGAGCAGAAC AAAAAGGGGT ACATTAGAGA TCTCCATAAC AGTAAAATTC 
     1921 ACCAAGCTAT CACATTACAC CCCAACAAAA ACCCACCCTA CCAGTACAGG CTCCACAGCT 
     1981 ACATGCTGAG CCGCAAGATA TCCGAGCTCC GCCATCGCAC AATACAGCTG CACCGCGAAA 
     2041 TTGTCCTGAT GAGCAAATAC AGCAACACAG AAATTCATAA AGAGGACCTC CAGCTGGGAA 
     2101 TCCCTCCCTC CTTCATGAGG TTTCAGCCCC GCCAGCGAGA GGAGATTCTG GAATGGGAGT 
     2161 TTCTGACTGG AAAATACTTG TATTCGGCAG TTGACGGCCA GCCCCCTCGA AGAGGAATGG 
     2221 ACTCCGCCCA GAGGGAAGCC TTGGACGACA TTGTCATGCA GGTCATGGAG ATGATCAATG 
     2281 CCAACGCCAA GACCAGAGGG CGCATCATTG ACTTCAAAGA GATCCAGTAC GGCTACCGCC 
     2341 GGGTGAACCC CATGTATGGG GCTGAGTACA TCCTGGACCT GCTGCTTCTG TACAAAAAGC 
     2401 ACAAAGGGAA GAAAATGACG GTCCCTGTGA GGAGGCACGC GTATTTACAG CAGACTTTCA 
     2461 GCAAAATCCA GTTTGTGGAG CATGAGGAGC TGGATGCACA AGAGTTGGCC AAGAGAATCA 
     2521 ATCAGGAATC TGGATCCTTG TCCTTTCTCT CAAACTCCCT GAAGAAGCTC GTCCCCTTTC 
     2581 AGCTCCCTGG GTCGAAGAGT GAGCACAAAG AACCCAAAGA TAAAAAGATA AACATACTGA 
     2641 TTCCTTTGTC TGGGCGTTTC GACATGTTTG TGAGATTTAT GGGAAACTTT GAGAAGACGT 
     2701 GTCTTATCCC CAATCAGAAC GTCAAGCTCG TGGTTCTGCT TTTCAATTCT GACTCCAACC 
     2761 CTGACAAGGC CAAACAAGTT GAACTGATGA GAGATTACCG CATTAAGTAC CCTAAAGCCG 
     2821 ACATGCAGAT TTTGCCTGTG TCTGGAGAGT TTTCAAGAGC CCTGGCCCTG GAAGTAGGAT 
     2881 CCTCCCAGTT TAACAATGAA TCTTTGCTCT TCTTCTGCGA CGTCGACCTC GTGTTTACTA 
     2941 CAGAATTCCT TCAGCGATGT CGAGCAAATA CAGTTCTGGG CCAACAAATA TATTTTCCAA 
     3001 TCATCTTCAG CCAGTATGAC CCAAAGATTG TTTATAGTGG GAAAGTTCCC AGTGACAACC 
     3061 ATTTTGCCTT TACTCAGAAA ACTGGCTTCT GGAGAAACTA TGGGTTTGGC ATCACGTGTA 
     3121 TTTATAAGGG AGATCTTGTC CGAGTGGGTG GCTTTGATGT TTCCATCCAA GGCTGGGGGC 
     3181 TGGAGGATGT GGACCTTTTC AACAAGGTTG TCCAGGCAGG TTTGAAGACG TTTAGGAGCC 
     3241 AGGAAGTAGG AGTAGTCCAC GTCCACCATC CTGTCTTTTG TGATCCCAAT CTTGACCCCA 
     3301 AACAGTACAA AATGTGCTTG GGGTCCAAAG CATCGACCTA TGGGTCCACC CAGCAGCTGG 
     3361 CTGAGATGTG GCTGGAAAAA AATGATCCAA GTTACAGTAA AAGCAGCAAT AATAATGGCT 
     3421 CAGTGAGGAC AGCCACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3481 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3541 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3601 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3661 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3721 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3781 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3841 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3901 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     3961 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4021 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4081 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4141 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4201 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4261 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4321 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4381 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4441 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4501 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4561 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4621 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4681 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4741 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4801 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4861 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4921 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     4981 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5041 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5101 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5161 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5221 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5281 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5341 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5401 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5461 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5521 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5581 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5641 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5701 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5761 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5821 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     5881 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     5941 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6001 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6061 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6121 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6181 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6241 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6301 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6361 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6421 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6481 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6541 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6601 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6661 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6721 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6781 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6841 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     6901 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     6961 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7021 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7081 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7141 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7201 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7261 CAAAAATTTA ACGCGAATTT T
//