LOCUS       RC207801                8433 bp    DNA     circular  SYN 
DEFINITION  CILP (Myc-DDK-tagged)-Human cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP)
ACCESSION   NM_003613
VERSION     NM_003613.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4590..4681 
                     /label="Myc-DDK"  
     misc_feature    4711..5296 
                     /label="PolyA signal"  
     misc_feature    4768..4787 
                     /label="Restriction Enzyme"  
     misc_feature    5572..6244 
                     /label="ColE1"  
     misc_feature    6674..7468 
                     /label="Kan/Neo"  
     misc_feature    7503..7805 
                     /label="SV40 ori"  
     misc_feature    7891..8368 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4572..4578 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4572 
                     /label="CILP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGGGACC AAGGCCTGGG TGTTCTCCTT CCTGGTCCTG GAAGTCACAT 
     1081 CTGTGTTGGG GAGACAGACG ATGCTCACCC AGTCAGTAAG AAGAGTCCAG CCTGGGAAGA 
     1141 AGAACCCCAG CATCTTTGCC AAGCCTGCCG ACACCCTGGA GAGCCCTGGT GAGTGGACAA 
     1201 CATGGTTCAA CATCGACTAC CCAGGCGGGA AGGGCGACTA TGAGCGGCTG GACGCCATTC 
     1261 GCTTCTACTA TGGGGACCGT GTATGTGCCC GTCCCCTGCG GCTAGAGGCT CGGACCACTG 
     1321 ACTGGACACC TGCGGGCAGC ACTGGCCAGG TGGTCCATGG TAGTCCCCGT GAGGGTTTCT 
     1381 GGTGCCTCAA CAGGGAGCAG CGGCCTGGCC AGAACTGCTC TAATTACACC GTACGCTTCC 
     1441 TCTGCCCACC AGGATCCCTG CGCCGAGACA CAGAGCGCAT CTGGAGCCCA TGGTCTCCCT 
     1501 GGAGCAAGTG CTCAGCTGCC TGTGGTCAGA CTGGGGTCCA GACTCGCACA CGCATTTGCT 
     1561 TGGCAGAGAT GGTGTCGCTG TGCAGTGAGG CCAGCGAAGA GGGTCAGCAC TGCATGGGCC 
     1621 AGGACTGTAC AGCCTGTGAC CTGACCTGCC CAATGGGCCA GGTGAATGCT GACTGTGATG 
     1681 CCTGCATGTG CCAGGACTTC ATGCTTCATG GGGCTGTCTC CCTTCCCGGA GGTGCCCCAG 
     1741 CCTCAGGGGC TGCTATCTAC CTCCTGACCA AGACGCCGAA GCTGCTGACC CAGACAGACA 
     1801 GTGATGGGAG ATTCCGAATC CCTGGCTTGT GCCCTGATGG CAAAAGCATC CTGAAGATCA 
     1861 CAAAGGTCAA GTTTGCCCCC ATTGTACTCA CAATGCCCAA GACTAGCCTG AAGGCAGCCA 
     1921 CCATCAAGGC AGAGTTTGTG AGGGCAGAGA CTCCATACAT GGTGATGAAC CCTGAGACAA 
     1981 AAGCACGGAG AGCTGGGCAG AGCGTGTCTC TGTGCTGTAA GGCCACAGGG AAGCCCAGGC 
     2041 CAGACAAGTA TTTTTGGTAT CATAATGACA CATTGCTGGA TCCTTCCCTC TACAAGCATG 
     2101 AGAGCAAGCT GGTGCTGAGG AAACTGCAGC AGCACCAGGC TGGGGAGTAC TTTTGCAAGG 
     2161 CCCAGAGTGA TGCTGGGGCT GTGAAGTCCA AGGTTGCCCA GCTGATTGTC ATAGCATCTG 
     2221 ATGAGACTCC TTGCAACCCA GTTCCTGAGA GCTATCTTAT CCGGCTGCCC CATGATTGCT 
     2281 TTCAGAATGC CACCAACTCC TTCTACTATG ACGTGGGACG CTGCCCTGTT AAGACTTGTG 
     2341 CAGGGCAGCA GGATAATGGG ATCAGGTGCC GTGATGCTGT GCAGAACTGC TGTGGCATCT 
     2401 CCAAGACAGA GGAAAGGGAG ATCCAGTGCA GTGGCTACAC GCTACCCACC AAGGTGGCCA 
     2461 AGGAGTGCAG CTGCCAGCGG TGTACGGAAA CTCGGAGCAT CGTGCGGGGC CGTGTCAGTG 
     2521 CTGCTGACAA TGGGGAGCCC ATGCGCTTTG GCCATGTGTA CATGGGGAAC AGCCGTGTAA 
     2581 GCATGACTGG CTACAAGGGC ACTTTCACCC TCCATGTCCC CCAGGACACT GAGAGGCTGG 
     2641 TGCTCACATT TGTGGACAGG CTGCAGAAGT TTGTCAACAC CACCAAAGTG CTACCTTTCA 
     2701 ACAAGAAGGG GAGTGCCGTG TTCCATGAAA TCAAGATGCT TCGTCGGAAA GAGCCCATCA 
     2761 CTTTGGAAGC CATGGAGACC AACATCATCC CCCTGGGGGA AGTGGTTGGT GAAGACCCCA 
     2821 TGGCTGAACT GGAGATTCCA TCCAGGAGTT TCTACAGGCA GAATGGGGAG CCCTACATAG 
     2881 GAAAAGTGAA GGCCAGTGTG ACCTTCCTGG ATCCCCGGAA TATTTCCACA GCCACAGCTG 
     2941 CCCAGACTGA CCTGAACTTC ATCAATGACG AAGGAGACAC TTTCCCCCTT CGGACGTATG 
     3001 GCATGTTCTC TGTGGACTTC AGAGATGAGG TCACCTCAGA GCCACTTAAT GCTGGCAAAG 
     3061 TGAAGGTCCA CCTTGACTCG ACCCAGGTCA AGATGCCAGA GCACATATCC ACAGTGAAAC 
     3121 TCTGGTCACT CAATCCAGAC ACAGGGCTGT GGGAGGAGGA AGGTGATTTC AAATTTGAAA 
     3181 ATCAAAGGAG GAACAAAAGA GAAGACAGAA CCTTCCTGGT GGGCAACCTG GAGATTCGTG 
     3241 AGAGGAGGCT CTTTAACCTG GATGTTCCTG AAAGCAGGCG GTGCTTTGTT AAGGTGAGGG 
     3301 CCTACCGGAG TGAGAGGTTC TTGCCTAGTG AGCAGATCCA GGGGGTTGTG ATCTCCGTGA 
     3361 TTAACCTGGA GCCTAGAACT GGCTTCTTGT CCAACCCTAG GGCCTGGGGC CGCTTTGACA 
     3421 GTGTCATCAC AGGCCCCAAC GGGGCCTGTG TGCCTGCCTT CTGTGATGAC CAGTCCCCTG 
     3481 ATGCCTACTC TGCCTATGTC TTGGCAAGCC TGGCTGGGGA GGAACTGCAA GCAGTGGAGT 
     3541 CTTCTCCTAA ATTCAACCCA AATGCAATTG GCGTCCCTCA GCCCTATCTC AACAAGCTCA 
     3601 ACTACCGTCG GACGGACCAT GAGGATCCAC GGGTTAAAAA GACAGCTTTC CAGATTAGCA 
     3661 TGGCCAAGCC AAGGCCCAAC TCAGCTGAGG AGAGCAATGG GCCCATCTAT GCCTTTGAGA 
     3721 ACCTCCGGGC ATGTGAAGAG GCACCACCCA GTGCAGCCCA CTTCCGGTTC TACCAGATTG 
     3781 AGGGGGATCG ATATGACTAC AACACAGTCC CCTTCAACGA AGATGACCCT ATGAGCTGGA 
     3841 CTGAAGACTA TCTGGCATGG TGGCCAAAGC CGATGGAATT CAGGGCCTGC TATATCAAGG 
     3901 TGAAGATTGT GGGGCCACTG GAAGTGAATG TGCGATCCCG CAACATGGGG GGCACTCATC 
     3961 GGCGGACAGT GGGGAAGCTG TATGGAATCC GAGATGTGAG GAGCACTCGG GACAGGGACC 
     4021 AGCCCAATGT CTCAGCTGCC TGTCTGGAGT TCAAGTGCAG TGGGATGCTC TATGATCAGG 
     4081 ACCGTGTGGA CCGCACCCTG GTGAAGGTCA TCCCCCAGGG CAGCTGCCGT CGAGCCAGTG 
     4141 TGAACCCCAT GCTGCATGAG TACCTGGTCA ACCACTTGCC ACTTGCAGTC AACAACGACA 
     4201 CCAGTGAGTA CACCATGCTG GCACCCTTGG ACCCACTGGG CCACAACTAT GGCATCTACA 
     4261 CTGTCACTGA CCAGGACCCT CGCACGGCCA AGGAGATCGC GCTCGGCCGG TGCTTTGATG 
     4321 GCACATCCGA TGGCTCCTCC AGAATCATGA AGAGCAATGT GGGAGTAGCC CTCACCTTCA 
     4381 ACTGTGTAGA GAGGCAAGTA GGCCGCCAGA GTGCCTTCCA GTACCTCCAA AGCACCCCAG 
     4441 CCCAGTCCCC TGCTGCAGGC ACTGTCCAAG GAAGAGTGCC CTCGAGGAGG CAGCAGCGAG 
     4501 CGAGCAGGGG TGGCCAGCGC CAGAGTGGAG TGGTGGCCTC TCTGAGATTT CCTAGAGTTG 
     4561 CTCAACAGCC CCTGATCAAC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4621 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4681 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4741 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4801 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4861 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4921 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4981 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5041 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5101 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5161 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5221 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5281 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5341 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5401 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5461 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5521 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5581 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5641 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5701 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5761 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5821 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5881 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5941 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6001 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6061 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6121 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6181 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6241 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6301 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6361 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6421 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6481 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6541 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6601 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6661 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6721 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6781 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6841 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6901 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6961 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7021 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7081 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7141 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7201 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7261 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7321 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7381 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7441 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7501 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7561 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7621 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7681 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7741 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7801 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7861 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7921 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7981 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8041 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8101 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8161 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8221 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8281 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8341 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8401 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//