LOCUS       RC207616L1                9216 bp    DNA     circular  SYN 
DEFINITION  MAP3K14 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 14 (MAP3K14)
ACCESSION   NM_003954
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7507..7687 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8415..9074 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7846..8414 
                     /label="ColE1"  
     misc_feature    6382..6402 
                     /label="LR50 primer"  
     misc_feature    6179..6250 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6391..6982 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6161..6167 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6161 
                     /label="MAP3K14"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCAGTGATG GAAATGGCCT GCCCAGGTGC 
     3361 CCCTGGCTCA GCAGTGGGGC AGCAGAAGGA ACTCCCCAAA GCCAAGGAGA AGACGCCGCC 
     3421 ACTGGGGAAG AAACAGAGCT CCGTCTACAA GCTTGAGGCC GTGGAGAAGA GCCCTGTGTT 
     3481 CTGCGGAAAG TGGGAGATCC TGAATGACGT GATTACCAAG GGCACAGCCA AGGAAGGCTC 
     3541 CGAGGCAGGG CCAGCTGCCA TCTCTATCAT CGCCCAGGCT GAGTGTGAGA ATAGCCAAGA 
     3601 GTTCAGCCCC ACCTTTTCAG AACGCATTTT CATCGCTGGG TCCAAACAGT ACAGCCAGTC 
     3661 CGAGAGTCTT GATCAGATCC CCAACAATGT GGCCCATGCT ACAGAGGGCA AAATGGCCCG 
     3721 TGTGTGTTGG AAGGGAAAGC GTCGCAGCAA AGCCCGGAAG AAACGGAAGA AGAAGAGCTC 
     3781 AAAGTCCCTG GCTCATGCAG GAGTGGCCTT GGCCAAACCC CTCCCCAGGA CCCCTGAGCA 
     3841 GGAGAGCTGC ACCATCCCAG TGCAGGAGGA TGAGTCTCCA CTCGGCGCCC CATATGTTAG 
     3901 AAACACCCCG CAGTTCACCA AGCCTCTGAA GGAACCAGGC CTTGGGCAAC TCTGTTTTAA 
     3961 GCAGCTTGGC GAGGGCCTAC GGCCGGCTCT GCCTCGATCA GAACTCCACA AACTGATCAG 
     4021 CCCCTTGCAA TGTCTGAACC ACGTGTGGAA ACTGCACCAC CCCCAGGACG GAGGCCCCCT 
     4081 GCCCCTGCCC ACGCACCCCT TCCCCTATAG CAGACTGCCT CATCCCTTCC CATTCCACCC 
     4141 TCTCCAGCCC TGGAAACCTC ACCCTCTGGA GTCCTTCCTG GGCAAACTGG CCTGTGTAGA 
     4201 CAGCCAGAAA CCCTTGCCTG ACCCACACCT GAGCAAACTG GCCTGTGTAG ACAGTCCAAA 
     4261 GCCCCTGCCT GGCCCACACC TGGAGCCCAG CTGCCTGTCT CGTGGTGCCC ATGAGAAGTT 
     4321 TTCTGTGGAG GAATACCTAG TGCATGCTCT GCAAGGCAGC GTGAGCTCAG GCCAGGCCCA 
     4381 CAGCCTGACC AGCCTGGCCA AGACCTGGGC AGCAAGGGGC TCCAGATCCC GGGAGCCCAG 
     4441 CCCCAAAACT GAGGACAACG AGGGTGTCCT GCTCACTGAG AAACTCAAGC CAGTGGATTA 
     4501 TGAGTACCGA GAAGAAGTCC ACTGGGCCAC GCACCAGCTC CGCCTGGGCA GAGGCTCCTT 
     4561 CGGAGAGGTG CACAGGATGG AGGACAAGCA GACTGGCTTC CAGTGCGCTG TCAAAAAGGT 
     4621 GCGGCTGGAA GTATTTCGGG CAGAGGAGCT GATGGCATGT GCAGGATTGA CCTCACCCAG 
     4681 AATTGTCCCT TTGTATGGAG CTGTGAGAGA AGGGCCTTGG GTCAACATCT TCATGGAGCT 
     4741 GCTGGAAGGT GGCTCCCTGG GCCAGCTGGT CAAGGAGCAG GGCTGTCTCC CAGAGGACCG 
     4801 GGCCCTGTAC TACCTGGGCC AGGCCCTGGA GGGTCTGGAA TACCTCCACT CACGAAGGAT 
     4861 TCTGCATGGG GACGTCAAAG CTGACAACGT GCTCCTGTCC AGCGATGGGA GCCACGCAGC 
     4921 CCTCTGTGAC TTTGGCCATG CTGTGTGTCT TCAACCTGAT GGCCTGGGAA AGTCCTTGCT 
     4981 CACAGGGGAC TACATCCCTG GCACAGAGAC CCACATGGCT CCGGAGGTGG TGCTGGGCAG 
     5041 GAGCTGCGAC GCCAAGGTGG ATGTCTGGAG CAGCTGCTGT ATGATGCTGC ACATGCTCAA 
     5101 CGGCTGCCAC CCCTGGACTC AGTTCTTCCG AGGGCCGCTC TGCCTCAAGA TTGCCAGCGA 
     5161 GCCTCCGCCT GTGAGGGAGA TCCCACCCTC CTGCGCCCCT CTCACAGCCC AGGCCATCCA 
     5221 AGAGGGGCTG AGGAAAGAGC CCATCCACCG CGTGTCTGCA GCGGAGCTGG GAGGGAAGGT 
     5281 GAACCGGGCA CTACAGCAAG TGGGAGGTCT GAAGAGCCCT TGGAGGGGAG AATATAAAGA 
     5341 ACCAAGACAT CCACCGCCAA ATCAAGCCAA TTACCACCAG ACCCTCCATG CCCAGCCGAG 
     5401 AGAGCTTTCG CCAAGGGCCC CAGGGCCCCG GCCAGCTGAG GAGACAACAG GCAGAGCCCC 
     5461 TAAGCTCCAG CCTCCTCTCC CACCAGAGCC CCCAGAGCCA AACAAGTCTC CTCCCTTGAC 
     5521 TTTGAGCAAG GAGGAGTCTG GGATGTGGGA ACCCTTACCT CTGTCCTCCC TGGAGCCAGC 
     5581 CCCTGCCAGA AACCCCAGCT CACCAGAGCG GAAAGCAACC GTCCCGGAGC AGGAACTGCA 
     5641 GCAGCTGGAA ATAGAATTAT TCCTCAACAG CCTGTCCCAG CCATTTTCTC TGGAGGAGCA 
     5701 GGAGCAAATT CTCTCGTGCC TCAGCATCGA CAGCCTCTCC CTGTCGGATG ACAGTGAGAA 
     5761 GAACCCATCA AAGGCCTCTC AAAGCTCGCG GGACACCCTG AGCTCAGGCG TACACTCCTG 
     5821 GAGCAGCCAG GCCGAGGCTC GAAGCTCCAG CTGGAACATG GTGCTGGCCC GGGGGCGGCC 
     5881 CACCGACACC CCAAGCTATT TCAATGGTGT GAAAGTCCAA ATACAGTCTC TTAATGGTGA 
     5941 ACACCTGCAC ATCCGGGAGT TCCACCGGGT CAAAGTGGGA GACATCGCCA CTGGCATCAG 
     6001 CAGCCAGATC CCAGCTGCAG CCTTCAGCTT GGTGACCAAA GACGGGCAGC CTGTTCGCTA 
     6061 CGACATGGAG GTGCCAGACT CGGGCATCGA CCTGCAGTGC ACACTGGCCC CTGATGGCAG 
     6121 CTTCGCCTGG AGCTGGAGGG TCAAGCATGG CCAGCTGGAG AACAGGCCCA CGCGTACGCG 
     6181 GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA 
     6241 CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CTGTACAAGT AGGATTCGTC 
     6301 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATACATGT TTAAGGGTTC 
     6361 CGGTTCCACT AGGTACAATT CGATATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA 
     6421 ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC 
     6481 GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC 
     6541 TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT 
     6601 GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC 
     6661 TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC 
     6721 GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG 
     6781 GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT 
     6841 CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC 
     6901 CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT 
     6961 CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGTC GACCTCGATC GAGACCTAGA 
     7021 AAAACATGGA GCAATCACAA GTAGCAATAC AGCAGCTACC AATGCTGATT GTGCCTGGCT 
     7081 AGAAGCACAA GAGGAGGAGG AGGTGGGTTT TCCAGTCACA CCTCAGGTAC CTTTAAGACC 
     7141 AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA 
     7201 AGGGCTAATT CACTCCCAAC GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA 
     7261 AGGCTACTTC CCTGATTGGC AGAACTACAC ACCAGGGCCA GGGATCAGAT ATCCACTGAC 
     7321 CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCAAGAG AAGGTAGAAG AAGCCAATGA 
     7381 AGGAGAGAAC ACCCGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG 
     7441 AGAAGTATTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACATGG CCCGAGAGCT 
     7501 GCATCCGGAC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG 
     7561 CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT 
     7621 GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT 
     7681 GTGGAAAATC TCTAGCAGCA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     7741 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     7801 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     7861 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     7921 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     7981 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     8041 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     8101 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     8161 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     8221 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     8281 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     8341 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     8401 ACGTTAAGGG ATTTTGGTCA TGATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT 
     8461 AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA CGGCATGATG AACCTGAATC 
     8521 GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG 
     8581 GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA 
     8641 TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA 
     8701 CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT 
     8761 TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA 
     8821 ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA TACGGAACTC CGGATGAGCA 
     8881 TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT 
     8941 ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA 
     9001 ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA 
     9061 TATCCAGTGA TTTTTTTCTC CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     9121 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     9181 GTCCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGAC
//