LOCUS       RC207553                9051 bp    DNA     circular  SYN 
DEFINITION  POLR3A (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide A, 155kDa (POLR3A)
ACCESSION   NM_007055
VERSION     NM_007055.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5208..5299 
                     /label="Myc-DDK"  
     misc_feature    5329..5914 
                     /label="PolyA signal"  
     misc_feature    5386..5405 
                     /label="Restriction Enzyme"  
     misc_feature    6190..6862 
                     /label="ColE1"  
     misc_feature    7292..8086 
                     /label="Kan/Neo"  
     misc_feature    8121..8423 
                     /label="SV40 ori"  
     misc_feature    8509..8986 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5190..5196 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5190 
                     /label="POLR3A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGAAGGAG CAGTTCCGGG AGACGGATGT GGCCAAGAAA ATAAGCCACA 
     1081 TCTGTTTTGG AATGAAGTCA CCTGAGGAGA TGCGCCAGCA GGCGCACATC CAAGTTGTGA 
     1141 GTAAGAACCT GTACAGCCAG GACAACCAAC ATGCCCCCTT GCTATATGGG GTGCTCGACC 
     1201 ATAGGATGGG TACGAGTGAG AAGGATCGTC CATGTGAAAC CTGTGGGAAA AACTTGGCTG 
     1261 ACTGTCTAGG CCACTATGGG TATATCGACC TGGAGTTGCC GTGTTTTCAT GTAGGGTACT 
     1321 TCAGAGCAGT CATAGGCATC TTACAGATGA TCTGCAAAAC CTGCTGCCAC ATCATGCTGT 
     1381 CCCAAGAGGA GAAGAAGCAG TTTCTGGACT ATCTAAAGAG GCCCGGCCTG ACCTACCTTC 
     1441 AGAAGCGAGG ACTGAAAAAG AAAATCTCTG ACAAGTGCCG GAAGAAAAAC ATCTGCCATC 
     1501 ACTGTGGCGC TTTTAATGGT ACCGTAAAGA AGTGTGGACT GCTGAAAATA ATTCATGAGA 
     1561 AATACAAGAC CAACAAAAAA GTGGTGGATC CCATTGTATC AAATTTCCTT CAGTCTTTTG 
     1621 AAACAGCCAT TGAACATAAT AAAGAAGTGG AGCCTCTGCT GGGAAGGGCA CAGGAAAACT 
     1681 TGAATCCCTT AGTAGTTCTG AATTTATTTA AACGAATCCC AGCTGAAGAT GTTCCTCTAC 
     1741 TTCTGATGAA CCCAGAAGCC GGAAAGCCGT CTGATTTGAT TCTCACACGA CTTTTGGTGC 
     1801 CTCCTTTGTG TATCAGACCC TCCGTTGTGA GTGATTTGAA GTCTGGCACC AATGAAGATG 
     1861 ATCTGACAAT GAAACTGACA GAAATCATTT TCCTAAACGA TGTTATTAAA AAGCATCGGA 
     1921 TCTCAGGAGC CAAGACCCAG ATGATCATGG AGGACTGGGA TTTCCTGCAG CTGCAGTGTG 
     1981 CCCTCTACAT TAACAGTGAG CTCTCGGGCA TTCCCCTCAA CATGGCACCC AAGAAGTGGA 
     2041 CCAGAGGCTT CGTCCAACGC CTGAAGGGAA AACAGGGTCG ATTTAGAGGA AATCTCTCAG 
     2101 GAAAGAGAGT GGATTTTTCT GGCAGAACAG TCATCTCGCC CGACCCCAAC CTCCGGATTG 
     2161 ATGAGGTAGC TGTGCCAGTT CATGTGGCCA AAATTCTAAC TTTTCCTGAG AAGGTAAACA 
     2221 AAGCAAACAT CAATTTCTTG AGGAAACTGG TTCAAAACGG CCCTGAGGTT CACCCAGGAG 
     2281 CAAACTTCAT TCAGCAGAGA CATACGCAGA TGAAAAGGTT TTTGAAATAC GGAAATCGAG 
     2341 AAAAGATGGC TCAAGAGCTC AAGTATGGTG ACATCGTAGA GAGACACCTC ATCGATGGAG 
     2401 ATGTGGTGCT GTTCAATCGG CAGCCCTCGC TGCACAAATT GAGCATTATG GCTCATCTGG 
     2461 CCAGGGTCAA GCCCCACCGG ACCTTCAGAT TTAATGAGTG TGTCTGTACA CCCTATAATG 
     2521 CTGACTTTGA TGGTGATGAA ATGAACCTTC ATCTTCCTCA AACAGAAGAA GCTAAAGCAG 
     2581 AGGCCCTTGT TCTGATGGGG ACTAAAGCAA ATCTTGTAAC CCCGAGGAAT GGGGAACCGC 
     2641 TGATTGCTGC TATTCAGGAT TTTCTAACAG GTGCCTATCT CCTCACTCTC AAGGACACTT 
     2701 TCTTTGATCG AGCCAAGGCT TGCCAAATCA TTGCTTCAAT ACTGGTTGGC AAGGATGAGA 
     2761 AAATTAAAGT TCGCCTCCCA CCGCCTACAA TCCTAAAGCC TGTCACCCTG TGGACGGGAA 
     2821 AGCAGATCTT CAGTGTCATC CTCAGGCCTA GCGATGACAA TCCAGTGAGG GCCAACCTGC 
     2881 GAACCAAGGG CAAGCAGTAC TGTGGCAAAG GGGAAGATCT CTGTGCCAAT GATTCCTATG 
     2941 TTACAATCCA GAACAGTGAG TTGATGAGTG GCAGCATGGA CAAAGGAACC CTAGGGTCAG 
     3001 GATCCAAGAA CAATATTTTT TACATTTTGC TGCGAGACTG GGGACAGAAT GAAGCTGCAG 
     3061 ATGCCATGTC ACGGCTCGCC AGGCTGGCTC CTGTCTACCT GTCTAACCGT GGTTTCTCAA 
     3121 TTGGGATCGG TGATGTCACA CCTGGCCAAG GACTGCTGAA GGCCAAGTAT GAGTTGCTGA 
     3181 ATGCCGGCTA CAAGAAATGT GATGAGTACA TCGAAGCCCT GAACACGGGC AAGCTGCAGC 
     3241 AGCAGCCTGG CTGCACTGCT GAGGAGACCC TGGAGGCACT GATCCTGAAG GAGCTGTCTG 
     3301 TGATCCGTGA CCACGCTGGC AGTGCCTGCC TCCGGGAGCT GGACAAGAGC AACAGCCCCC 
     3361 TCACCATGGC TCTGTGCGGC TCCAAAGGTT CCTTCATTAA CATATCACAG ATGATTGCCT 
     3421 GTGTGGGACA GCAGGCCATC AGTGGCTCTC GAGTGCCAGA CGGCTTTGAA AACAGGTCCT 
     3481 TGCCTCATTT TGAAAAACAC TCAAAGCTCC CAGCTGCCAA AGGCTTTGTG GCTAATAGCT 
     3541 TTTATTCCGG TTTGACACCA ACTGAGTTTT TCTTCCACAC AATGGCCGGC CGGGAAGGTC 
     3601 TAGTCGACAC GGCTGTAAAG ACAGCTGAAA CGGGATACAT GCAGCGAAGG CTTGTCAAAT 
     3661 CTCTTGAAGA TCTTTGCTCC CAGTATGATC TGACAGTCCG AAGCTCTACT GGCGATATTA 
     3721 TCCAGTTCAT TTATGGAGGA GATGGCTTAG ATCCTGCAGC TATGGAGGGA AAAGATGAAC 
     3781 CTTTGGAGTT TAAAAGGGTT CTGGACAACA TCAAAGCAGT CTTCCCGTGT CCCAGTGAGC 
     3841 CTGCTCTCAG CAAAAACGAG CTGATCCTGA CCACAGAGTC CATCATGAAG AAGAGTGAGT 
     3901 TCCTCTGCTG CCAGGACAGC TTCCTGCAGG AAATAAAAAA ATTCATTAAG GGGGTCTCTG 
     3961 AGAAGATCAA GAAAACCAGA GATAAATATG GCATCAATGA TAACGGCACA ACAGAGCCCC 
     4021 GTGTGCTGTA CCAGCTGGAC CGCATCACCC CCACCCAAGT AGAAAAGTTT CTGGAGACCT 
     4081 GTAGGGACAA GTACATGAGG GCACAGATGG AGCCAGGTTC TGCAGTGGGT GCTCTGTGTG 
     4141 CCCAGAGCAT TGGTGAGCCA GGCACCCAGA TGACCCTGAA GACTTTCCAC TTTGCAGGTG 
     4201 TGGCCTCCAT GAACATCACC CTGGGCGTGC CCCGGATTAA AGAGATCATC AACGCTTCCA 
     4261 AGGCCATCAG CACTCCAATT ATCACAGCAC AGCTAGACAA GGATGACGAC GCGGATTATG 
     4321 CTCGCCTCGT GAAAGGGAGA ATTGAGAAAA CCCTCTTGGG AGAGATTTCC GAGTATATTG 
     4381 AAGAAGTGTT TCTTCCTGAT GACTGCTTTA TTCTCGTCAA GCTCTCCCTG GAACGGATTA 
     4441 GGCTTCTGAG ACTGGAAGTG AACGCTGAGA CAGTGAGATA TTCCATCTGC ACATCCAAGC 
     4501 TCCGTGTGAA GCCCGGTGAT GTGGCTGTTC ATGGTGAGGC TGTGGTGTGT GTCACCCCCA 
     4561 GAGAGAACAG CAAGAGCTCC ATGTACTACG TGCTGCAGTT CCTGAAAGAG GATCTCCCCA 
     4621 AGGTGGTGGT GCAGGGCATT CCAGAGGTGT CCAGAGCTGT CATCCACATT GACGAGCAGA 
     4681 GTGGAAAGGA GAAGTACAAG CTTCTGGTGG AAGGTGATAA CCTGCGGGCA GTCATGGCCA 
     4741 CACACGGTGT GAAGGGCACC CGAACCACCT CCAATAACAC CTATGAGGTG GAGAAAACTC 
     4801 TGGGCATCGA GGCCGCCCGG ACAACGATCA TCAATGAAAT CCAGTACACC ATGGTGAACC 
     4861 ACGGCATGAG CATCGACAGG AGGCACGTGA TGCTGCTCTC CGACCTCATG ACCTACAAGG 
     4921 GTGAAGTCCT GGGCATCACT AGGTTTGGCC TGGCCAAGAT GAAGGAGAGT GTGCTGATGC 
     4981 TGGCCTCCTT TGAGAAGACG GCTGACCATC TCTTTGACGC TGCCTACTTC GGGCAGAAGG 
     5041 ACTCTGTGTG TGGGGTGTCT GAGTGCATCA TCATGGGAAT CCCAATGAAC ATTGGAACCG 
     5101 GGCTCTTCAA GCTGCTTCAC AAGGCTGACA GGGACCCGAA CCCTCCCAAG AGGCCCCTGA 
     5161 TCTTCGACAC AAATGAATTC CACATCCCCC TTGTCACAAC GCGTACGCGG CCGCTCGAGC 
     5221 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5281 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5341 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5401 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5461 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5521 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5581 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5641 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5701 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5761 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5821 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5881 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5941 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6001 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6061 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6121 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6181 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6241 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6301 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6361 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6421 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6481 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6541 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6601 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6661 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6721 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6781 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6841 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6901 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6961 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7021 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7081 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7141 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7201 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7261 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7321 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7381 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7441 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7501 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7561 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7621 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7681 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7741 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7801 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7861 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7921 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7981 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8041 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8101 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8161 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8221 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8281 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8341 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8401 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8461 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8521 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8581 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8641 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8701 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8761 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8821 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8881 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8941 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9001 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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