LOCUS       RC207550                7026 bp    DNA     circular  SYN 
DEFINITION  POLI (Myc-DDK-tagged)-Human polymerase (DNA directed) iota (POLI)
ACCESSION   NM_007195
VERSION     NM_007195.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3183..3274 
                     /label="Myc-DDK"  
     misc_feature    3304..3889 
                     /label="PolyA signal"  
     misc_feature    3361..3380 
                     /label="Restriction Enzyme"  
     misc_feature    4165..4837 
                     /label="ColE1"  
     misc_feature    5267..6061 
                     /label="Kan/Neo"  
     misc_feature    6096..6398 
                     /label="SV40 ori"  
     misc_feature    6484..6961 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3165..3171 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3165 
                     /label="POLI"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACTGGCG GACGTGGGGG CGGCAGCCAG CTCGCAGGGA GTTCATGATC 
     1081 AAGTGTTGCC CACACCAAAT GCTTCATCCA GAGTCATAGT ACATGTGGAT CTGGATTGCT 
     1141 TTTATGCACA AGTAGAAATG ATCTCAAATC CAGAGCTAAA AGACAAACCT TTAGGGGTTC 
     1201 AACAGAAATA TTTGGTGGTT ACCTGCAACT ATGAAGCTAG GAAACTTGGA GTTAAGAAAC 
     1261 TTATGAATGT CAGAGATGCA AAAGAAAAGT GTCCACAGTT GGTATTAGTT AATGGAGAAG 
     1321 ACCTGACCCG CTACAGAGAA ATGTCTTATA AGGTTACAGA ATTACTGGAA GAATTTAGTC 
     1381 CAGTTGTTGA GAGACTTGGA TTTGATGAAA ATTTTGTGGA TCTAACAGAA ATGGTTGAGA 
     1441 AGAGACTACA GCAGCTGCAA AGTGATGAAC TTTCTGCGGT GACTGTGTCG GGTCATGTAT 
     1501 ACAATAATCA GTCTATAAAC CTGCTTGACG TCTTGCACAT CAGACTACTT GTTGGATCTC 
     1561 AGATTGCAGC AGAGATGCGG GAAGCCATGT ATAATCAGTT GGGGCTCACT GGCTGTGCTG 
     1621 GAGTGGCTTC TAATAAACTG TTGGCAAAAT TAGTTTCTGG TGTCTTTAAA CCAAATCAAC 
     1681 AAACAGTCTT ATTACCTGAA AGTTGTCAAC ATCTTATTCA TAGTTTGAAT CACATAAAGG 
     1741 AAATACCTGG TATTGGCTAT AAAACTGCCA AATGTCTTGA AGCACTGGGT ATCAATAGTG 
     1801 TGCGTGATCT CCAAACCTTT TCACCCAAAA TTTTAGAAAA AGAATTAGGA ATTTCAGTTG 
     1861 CTCAGCGTAT CCAAAAGCTC AGTTTTGGAG AGGATAACTC CCCTGTGATA CTCTCAGGAC 
     1921 CACCTCAGTC CTTTAGTGAA GAAGATTCAT TTAAAAAATG TTCATCTGAA GTTGAAGCTA 
     1981 AAAATAAGAT TGAAGAACTA CTTGCTAGTC TTTTAAACAG AGTATGCCAA GATGGAAGGA 
     2041 AGCCTCATAC AGTGAGATTA ATAATCCGTC GGTATTCCTC TGAGAAGCAC TATGGTCGTG 
     2101 AGAGTCGTCA GTGCCCTATT CCTTCACATG TAATTCAGAA ATTAGGGACA GGAAATTATG 
     2161 ATGTGATGAC CCCAATGGTT GATATACTTA TGAAACTTTT TCGAAATATG GTGAATGTGA 
     2221 AGATGCCATT TCACCTTACC CTTCTAAGTG TGTGCTTCTG CAACCTTAAA GCACTAAATA 
     2281 CTGCTAAGAA AGGGCTTATT GATTATTATT TAATGCCATC ATTATCAACT ACTTCACGCT 
     2341 CTGGCAAGCA CAGTTTTAAA ATGAAAGACA CTCATATGGA AGATTTTCCC AAAGACAAAG 
     2401 AAACAAACCG GGATTTCCTA CCAAGTGGAA GAATTGAAAG TACAAGAACT AGGGAGTCTC 
     2461 CACTAGATAC CACAAATTTT TCTAAAGAAA AAGACATTAA TGAATTCCCA CTCTGTTCAC 
     2521 TTCCTGAAGG TGTTGACCAA GAAGTCTTCA AGCAGCTTCC AGTAGATATT CAAGAAGAAA 
     2581 TCCTTTCTGG AAAATCTAGG GAAAAATTTC AAGGGAAAGG AAGTGTGAGT TGTCCATTAC 
     2641 ATGCCTCTAG AGGAGTATTA TCTTTCTTTT CTAAAAAACA AATGCAAGAT ATTCCCATAA 
     2701 ATCCTAGAGA TCATTTATCC AGTAGCAAAC AGGTATCCTC TGTATCTCCT TGTGAACCGG 
     2761 GAACATCAGG CTTTAATAGC AGTAGTTCTT CTTACATGTC TAGCCAAAAG GATTATTCAT 
     2821 ATTATTTAGA TAATAGATTA AAAGATGAAC GAATAAGTCA AGGACCTAAA GAACCTCAAG 
     2881 GATTCCACTT TACAAATTCA AACCCTGCTG TGTCTGCTTT TCATTCATTT CCAAACTTGC 
     2941 AGAGTGAGCA ACTTTTCTCC AGAAACCACA CTACAGATAG CCATAAGCAA ACAGTAGCAA 
     3001 CAGACTCTCA TGAAGGACTT ACAGAAAATA GAGAGCCAGA TTCTGTTGAT GAGAAAATTA 
     3061 CTTTCCCTTC TGACATTGAT CCTCAAGTTT TCTATGAACT ACCAGAAGCA GTACAAAAGG 
     3121 AACTGCTGGC AGAGTGGAAG AGAGCAGGAT CAGATTTCCA CATTGGACAT AAAACGCGTA 
     3181 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3241 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3301 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3361 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3421 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3481 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3541 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3601 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3661 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3721 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     3781 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     3841 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     3901 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     3961 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4021 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4081 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4141 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4201 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4261 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4321 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4381 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4441 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4501 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4561 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4621 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4681 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     4741 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     4801 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     4861 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     4921 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     4981 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5041 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5101 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5161 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5221 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5281 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5341 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5401 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5461 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5521 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5581 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5641 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     5701 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     5761 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     5821 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     5881 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     5941 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6001 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6061 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6121 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6181 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6241 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6301 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6361 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6421 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6481 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6541 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6601 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6661 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     6721 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     6781 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     6841 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     6901 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     6961 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7021 AATTTT
//