LOCUS       RC207485L2                7989 bp    DNA     circular  SYN 
DEFINITION  PLSCR1 (Myc-DDK-tagged)-Human phospholipid scramblase 1 (PLSCR1)
ACCESSION   NM_021105
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6280..6460 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    7188..7847 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    6508..7127 
                     /label="ColE1"  
     misc_feature    5155..5175 
                     /label="LR50"  
     misc_feature    4295..5020 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    5164..5755 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4274..4280 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4274 
                     /label="PLSCR1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGACAAACAA AACTCACAGA TGAATGCTTC 
     3361 TCACCCGGAA ACAAACTTGC CAGTTGGGTA TCCTCCTCAG TATCCACCGA CAGCATTCCA 
     3421 AGGACCTCCA GGATATAGTG GCTACCCTGG GCCCCAGGTC AGCTACCCAC CCCCACCAGC 
     3481 CGGCCATTCA GGTCCTGGCC CAGCTGGCTT TCCTGTCCCA AATCAGCCAG TGTATAATCA 
     3541 GCCAGTATAT AATCAGCCAG TTGGAGCTGC AGGGGTACCA TGGATGCCAG CGCCACAGCC 
     3601 TCCATTAAAC TGTCCACCTG GATTAGAATA TTTAAGTCAG ATAGATCAGA TACTGATTCA 
     3661 TCAGCAAATT GAACTTCTGG AAGTTTTAAC AGGTTTTGAA ACTAATAACA AATATGAAAT 
     3721 TAAGAACAGC TTTGGACAGA GGGTTTACTT TGCAGCGGAA GATACTGATT GCTGTACCCG 
     3781 AAATTGCTGT GGGCCATCTA GACCTTTTAC CTTGAGGATT ATTGATAATA TGGGTCAAGA 
     3841 AGTCATAACT CTGGAGAGAC CACTAAGATG TAGCAGCTGT TGTTGTCCCT GCTGCCTTCA 
     3901 GGAGATAGAA ATCCAAGCTC CTCCTGGTGT ACCAATAGGT TATGTTATTC AGACTTGGCA 
     3961 CCCATGTCTA CCAAAGTTTA CAATTCAAAA TGAGAAAAGA GAGGATGTAC TAAAAATAAG 
     4021 TGGTCCATGT GTTGTGTGCA GCTGTTGTGG AGATGTTGAT TTTGAGATTA AATCTCTTGA 
     4081 TGAACAGTGT GTGGTTGGCA AAATTTCCAA GCACTGGACT GGAATTTTGA GAGAGGCATT 
     4141 TACAGACGCT GATAACTTTG GAATCCAGTT CCCTTTAGAC CTTGATGTTA AAATGAAAGC 
     4201 TGTAATGATT GGTGCCTGTT TCCTCATTGA CTTCATGTTT TTTGAAAGCA CTGGCAGCCA 
     4261 GGAACAAAAA TCAGGAGTGT GGACGCGTAC GCGGCCGCTC GAGATGAGCG GGGGCGAGGA 
     4321 GCTGTTCGCC GGCATCGTGC CCGTGCTGAT CGAGCTGGAC GGCGACGTGC ACGGCCACAA 
     4381 GTTCAGCGTG CGCGGCGAGG GCGAGGGCGA CGCCGACTAC GGCAAGCTGG AGATCAAGTT 
     4441 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTGGTGACCA CCCTCTGCTA 
     4501 CGGCATCCAG TGCTTCGCCC GCTACCCCGA GCACATGAAG ATGAACGACT TCTTCAAGAG 
     4561 CGCCATGCCC GAGGGCTACA TCCAGGAGCG CACCATCCAG TTCCAGGACG ACGGCAAGTA 
     4621 CAAGACCCGC GGCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 
     4681 GGGCAAGGAC TTCAAGGAGG ACGGCAACAT CCTGGGCCAC AAGCTGGAGT ACAGCTTCAA 
     4741 CAGCCACAAC GTGTACATCC GCCCCGACAA GGCCAACAAC GGCCTGGAGG CTAACTTCAA 
     4801 GACCCGCCAC AACATCGAGG GCGGCGGCGT GCAGCTGGCC GACCACTACC AGACCAACGT 
     4861 GCCCCTGGGC GACGGCCCCG TGCTGATCCC CATCAACCAC TACCTGAGCA CTCAGACCAA 
     4921 GATCAGCAAG GACCGCAACG AGGCCCGCGA CCACATGGTG CTCCTGGAGT CCTTCAGCGC 
     4981 CTGCTGCCAC ACCCACGGCA TGGACGAGCT GTACAGGTCC GGACTCAGAT AAGTTTAAAC 
     5041 GGCCGGCCGC GGTCTGTACA AGTAGGATTC GTCGAGGGAC CTAATAACTT CGTATAGCAT 
     5101 ACATTATACG AAGTTATACA TGTTTAAGGG TTCCGGTTCC ACTAGGTACA ATTCGATATC 
     5161 AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT 
     5221 AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT 
     5281 ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT 
     5341 TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC 
     5401 GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT 
     5461 TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA 
     5521 GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT 
     5581 CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC 
     5641 CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT 
     5701 CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG 
     5761 CATCGATACC GTCGACCTCG ATCGAGACCT AGAAAAACAT GGAGCAATCA CAAGTAGCAA 
     5821 TACAGCAGCT ACCAATGCTG ATTGTGCCTG GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG 
     5881 TTTTCCAGTC ACACCTCAGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT 
     5941 TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA 
     6001 AGATATCCTT GATCTGTGGA TCTACCACAC ACAAGGCTAC TTCCCTGATT GGCAGAACTA 
     6061 CACACCAGGG CCAGGGATCA GATATCCACT GACCTTTGGA TGGTGCTACA AGCTAGTACC 
     6121 AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA TGAAGGAGAG AACACCCGCT TGTTACACCC 
     6181 TGTGAGCCTG CATGGGATGG ATGACCCGGA GAGAGAAGTA TTAGAGTGGA GGTTTGACAG 
     6241 CCGCCTAGCA TTTCATCACA TGGCCCGAGA GCTGCATCCG GACTGTACTG GGTCTCTCTG 
     6301 GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC 
     6361 TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG 
     6421 TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GCATGTGAGC 
     6481 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6541 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6601 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6661 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6721 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6781 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6841 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6901 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6961 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     7021 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     7081 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     7141 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGATTAC 
     7201 GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG 
     7261 AAGCCATCAC AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT 
     7321 TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG 
     7381 TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA 
     7441 ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT 
     7501 ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA 
     7561 GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG 
     7621 TCTTTCATTG CCATACGGAA CTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA 
     7681 AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC 
     7741 AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT 
     7801 TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATACTC 
     7861 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7921 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7981 CCACCTGAC
//