LOCUS       RC207484                8466 bp    DNA     circular  SYN 
DEFINITION  MTMR4 (Myc-DDK-tagged)-Human myotubularin related protein 4 (MTMR4)
ACCESSION   NM_004687
VERSION     NM_004687.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4623..4714 
                     /label="Myc-DDK"  
     misc_feature    4744..5329 
                     /label="PolyA signal"  
     misc_feature    4801..4820 
                     /label="Restriction Enzyme"  
     misc_feature    5605..6277 
                     /label="ColE1"  
     misc_feature    6707..7501 
                     /label="Kan/Neo"  
     misc_feature    7536..7838 
                     /label="SV40 ori"  
     misc_feature    7924..8401 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4605..4611 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4605 
                     /label="MTMR4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTGAGGAG GGGCCCCCCA GCCTGGAGTA CATCCAAGCC AAGGATCTGT 
     1081 TCCCCCCCAA GGAACTAGTG AAGGAGGAAG AGAATCTTCA GGTCCCCTTC ACAGTGCTGC 
     1141 AGGGTGAGGG AGTAGAGTTC CTGGGCCGGG CAGCCGATGC CCTCATTGCC ATCTCTAACT 
     1201 ACCGGCTGCA TATCAAATTC AAGGACTCTG TCATCAACGT CCCCCTCCGG ATGATTGACA 
     1261 GTGTGGAGAG CCGTGATATG TTCCAGTTGC ACATTTCCTG CAAGGACTCC AAAGTGGTGA 
     1321 GGTGCCACTT CTCCACTTTT AAGCAGTGCC AAGAGTGGCT CTCACGGCTA AGCCGAGCCA 
     1381 CAGCAAGACC TGCCAAGCCT GAAGACCTCT TTGCCTTTGC CTACCATGCC TGGTGCCTGG 
     1441 GGCTGACCGA GGAGGACCAG CACACTCACC TATGTCAGCC AGGTGAGCAC ATACGTTGTC 
     1501 GACAGGAGGC GGAGCTTGCA AGGATGGGCT TTGACCTGCA GAACGTCTGG AGAGTCTCAC 
     1561 ACATCAACAG CAACTACAAA TTGTGCCCCA GTTACCCCCA GAAGCTGCTG GTTCCTGTGT 
     1621 GGATCACTGA CAAAGAGCTG GAGAACGTGG CTTCCTTCCG CTCCTGGAAG CGGATTCCCG 
     1681 TGGTTGTGTA TAGACACTTG CGCAATGGGG CTGCCATCGC CCGCTGCAGC CAGCCAGAGA 
     1741 TCAGCTGGTG GGGCTGGCGC AATGCTGATG ATGAGTACCT GGTCACGTCC ATTGCTAAAG 
     1801 CCTGTGCCCT GGACCCGGGG ACAAGGGCCA CTGGGGGCTC CCTCAGCACC GGGAATAATG 
     1861 ATACCAGCGA GGCGTGTGAT GCTGACTTTG ATTCTTCTCT GACTGCGTGC TCTGGAGTGG 
     1921 AGAGCACAGC AGCTCCTCAA AAGCTGCTGA TCCTGGATGC GCGATCCTAC ACGGCAGCAG 
     1981 TGGCCAACCG GGCCAAGGGT GGAGGCTGTG AATGTGAAGA GTACTATCCC AACTGTGAGG 
     2041 TCGTGTTCAT GGGAATGGCC AACATCCATG CCATCCGGAA CAGCTTTCAG TACCTCCGGG 
     2101 CTGTGTGTAG CCAGATGCCG GATCCTAGCA ACTGGTTGTC GGCACTGGAG AGTACCAAAT 
     2161 GGCTGCAGCA CTTGTCGGTG ATGCTAAAAG CAGCTGTGCT GGTGGCTAAT ACAGTAGACC 
     2221 GGGAAGGCCG GCCTGTGCTG GTACACTGCT CAGATGGCTG GGACCGCACA CCGCAGATCG 
     2281 TAGCCCTGGC CAAAATATTA CTGGACCCAT ATTACAGGAC GTTGGAGGGC TTCCAAGTGT 
     2341 TAGTGGAGTC TGACTGGCTG GATTTTGGGC ACAAGTTTGG AGATCGCTGT GGCCACCAAG 
     2401 AGAATGTGGA GGACCAAAAC GAACAATGCC CTGTGTTCCT CCAGTGGCTT GATTCTGTTC 
     2461 ATCAGTTGCT TAAGCAGTTC CCCTGCCTGT TTGAATTTAA TGAAGCATTC CTGGTAAAAC 
     2521 TGGTGCAACA CACATACTCC TGCCTCTACG GCACCTTCCT GGCCAACAAC CCCTGTGAGC 
     2581 GAGAGAAGCG CAACATCTAC AAGCGGACCT GCTCTGTGTG GGCGCTCCTT CGAGCTGGCA 
     2641 ATAAAAACTT TCATAACTTC CTCTACACAC CCAGCTCAGA CATGGTCCTG CATCCTGTTT 
     2701 GTCATGTCCG GGCCCTGCAC CTCTGGACAG CTGTTTATCT GCCAGCATCA TCTCCATGCA 
     2761 CACTTGGGGA AGAAAACATG GATCTTTACC TTTCCCCAGT GGCCCAGAGC CAGGAGTTCT 
     2821 CTGGCCGCTC TCTGGACAGA TTACCTAAAA CCAGATCCAT GGATGATCTT CTTTCTGCCT 
     2881 GTGACACAAG CAGCCCCCTG ACTCGTACAT CCAGTGACCC TAACCTGAAT AACCACTGTC 
     2941 AGGAGGTCAG GGTAGGCCTG GAGCCCTGGC ACAGCAATCC TGAGGGATCA GAGACAAGCT 
     3001 TTGTGGACTC TGGGGTAGGA GGGCCTCAGC AAACTGTAGG AGAAGTGGGT CTTCCTCCTC 
     3061 CTCTGCCCAG CAGCCAGAAA GACTACTTGA GCAATAAACC TTTCAAGAGT CACAAAAGCT 
     3121 GTTCTCCAAG TTACAAACTG CTTAATACCG CAGTGCCTCG GGAAATGAAG AGCAACACCT 
     3181 CTGATCCTGA GATCAAAGTC CTAGAAGAGA CTAAGGGACC AGCTCCAGAC CCTTCTGCCC 
     3241 AGGATGAGCT GGGTAGGACT TTAGATGGCA TAGGGGAGCC ACCTGAACAT TGTCCTGAAA 
     3301 CAGAAGCTGT CAGTGCACTC TCCAAGGTCA TTTCTAACAA GTGTGATGGA GTTTGTAATT 
     3361 TTCCTGAGTC TTCCCAGAAC TCTCCTACAG GTACGCCCCA ACAGGCCCAG CCAGACTCCA 
     3421 TGCTAGGTGT GCCCTCCAAG TGTGTTCTTG ATCACAGCCT CAGCACCGTT TGCAACCCAC 
     3481 CGAGTGCTGC CTGCCAAACT CCTCTAGACC CAAGCACTGA CTTCCTCAAC CAAGATCCCT 
     3541 CAGGGTCTGT GGCAAGTATC TCCCACCAGG AACAACTGAG TTCTGTGCCG GATCTGACCC 
     3601 ATGGGGAGGA AGACATTGGT AAAAGAGGAA ATAATAGGAA TGGGCAGTTA TTGGAAAATC 
     3661 CTCGCTTTGG GAAAATGCCA TTGGAATTGG TCCGGAAGCC AATTTCTCAG AGCCAGATCA 
     3721 GTGAGTTCTC TTTTCTAGGG TCCAACTGGG ACAGCTTCCA AGGGATGGTG ACTTCATTCC 
     3781 CAAGTGGGGA GGCCACCCCT CGGCGGCTGC TTTCCTATGG CTGTTGTAGC AAGAGGCCAA 
     3841 ACAGTAAGCA GATGCGGGCC ACAGGGCCCT GCTTTGGGGG CCAGTGGGCT CAGAGAGAAG 
     3901 GTGTGAAGTC ACCTGTCTGT TCTAGTCATT CCAATGGACA TTGTACTGGC CCAGGAGGAA 
     3961 AGAACCAGAT GTGGTTGTCC AGTCATCCAA AGCAAGTCTC TAGCACAAAG CCCGTTCCAC 
     4021 TGAACTGCCC TTCTCCAGTG CCTCCTCTGT ATTTGGATGA TGATGGACTC CCCTTTCCCA 
     4081 CGGATGTGAT CCAGCATAGG TTACGGCAAA TCGAAGCAGG GTACAAACAA GAGGTGGAGC 
     4141 AGCTACGTCG ACAGGTGCGT GAGCTTCAGA TGAGGCTGGA CATCCGTCAC TGCTGTGCCC 
     4201 CTCCAGCAGA GCCCCCCATG GACTATGAGG ATGATTTTAC ATGTTTGAAG GAGTCAGATG 
     4261 GCAGTGATAC TGAGGATTTT GGCTCTGATC ACAGTGAAGA CTGCCTTTCA GAAGCAAGCT 
     4321 GGGAACCTGT TGATAAGAAA GAGACTGAGG TGACTCGCTG GGTTCCAGAC CATATGGCAT 
     4381 CACACTGCTA TAACTGTGAC TGTGAATTCT GGTTGGCCAA ACGAAGACAC CATTGCAGAA 
     4441 ATTGTGGGAA TGTATTTTGT GCTGGATGCT GCCACCTGAA GCTGCCCATT CCTGATCAGC 
     4501 AACTCTATGA CCCAGTTCTC GTCTGTAACT CATGTTACGA ACACATTCAA GTCTCTCGTG 
     4561 CCAGGGAACT CATGAGCCAA CAGCTGAAGA AACCCATTGC TACAGCTTCC AGTACGCGTA 
     4621 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4681 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4741 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4801 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4861 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4921 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4981 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5041 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5101 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5161 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5221 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5281 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5341 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5401 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5461 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5521 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5581 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5641 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5701 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5761 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5821 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5881 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5941 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6001 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6061 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6121 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6181 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6241 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6301 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6361 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6421 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6481 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6541 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6601 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6661 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6721 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6781 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6841 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6901 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6961 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7021 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7081 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7141 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7201 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7261 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7321 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7381 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7441 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7501 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7561 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7621 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7681 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7741 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7921 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7981 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8041 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8101 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8161 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8221 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8281 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8341 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8401 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8461 AATTTT
//