LOCUS       RC207386                8733 bp    DNA     circular  SYN 
DEFINITION  COL20A1 (Myc-DDK-tagged)-Human collagen, type XX, alpha 1 (COL20A1)
ACCESSION   NM_020882
VERSION     NM_020882.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4890..4981 
                     /label="Myc-DDK"  
     misc_feature    5011..5596 
                     /label="PolyA signal"  
     misc_feature    5068..5087 
                     /label="Restriction Enzyme"  
     misc_feature    5872..6544 
                     /label="ColE1"  
     misc_feature    6974..7768 
                     /label="Kan/Neo"  
     misc_feature    7803..8105 
                     /label="SV40 ori"  
     misc_feature    8191..8668 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4872..4878 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4872 
                     /label="COL20A1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCTCCGGA GACCCTGCAC ACCTCGGCCT CTGCCTCTGG CTGTGGCTGG 
     1081 GCGCCACCCT GGGAAGAGAG CAAGTTCAAG CAAGCGGTCT CCTGAGGCTG GCTGTGCTGC 
     1141 CTGAGGACCG GCTGCAGATG AAGTGGAGAG AGTCGGAGGG GAGCGGCCTC GGCTACCTGG 
     1201 TGCAGGTGAA GCCCATGGCA GGGGACTCGG AACAGGAGGT GATACTGACC ACCAAGACCC 
     1261 CTAAGGCCAC AGTGGGGGGC CTGAGCCCCT CCAAGGGCTA CACCTTGCAG ATCTTCGAGC 
     1321 TCACTGGCTC TGGGCGCTTC CTGCTAGCTC GGAGGGAGTT TGTGATTGAG GATCTGAAGA 
     1381 GTAGCTCCCT GGACAGGAGC AGCCAGAGGC CCCTCGGCTC TGGAGCCCCG GAGCCCACCC 
     1441 CCTCCCACAC GGGGAGCCCA GACCCTGAGC AGGCTTCTGA GCCCCAAGTT GCCTTCACAC 
     1501 CAAGCCAGGA TCCGCGCACT CCTGCCGGCC CCCAGTTCCG CTGCCTGCCC CCCGTGCCTG 
     1561 CTGACATGGT CTTCCTGGTG GACGGGTCCT GGAGCATTGG CCACAGTCAC TTCCAGCAGG 
     1621 TCAAGGACTT CCTGGCCAGT GTCATCGCAC CCTTTGAAAT CGGGCCGGAT AAGGTCCAAG 
     1681 TAGGCCTGAC TCAGTACAGC GGGGATGCTC AGACTGAGTG GGACCTGAAC TCCCTCAGCA 
     1741 CCAAGGAACA GGTGCTGGCA GCTGTGCGCC GCCTCCGCTA CAAGGGGGGG AACACGTTCA 
     1801 CAGGCCTTGC CCTGACCCAC GTGCTGGGGC AGAACCTGCA GCCGGCGGCT GGCCTCCGTC 
     1861 CAGAGGCAGC CAAGGTGGTG ATTCTGGTGA CGGACGGCAA GTCCCAGGAC GATGTGCACA 
     1921 CTGCTGCCCG TGTCCTCAAG GACCTGGGCG TGAACGTCTT CGCTGTGGGT GTGAAGAACG 
     1981 CCGATGAGGC TGAGCTGAGG CTCCTGGCGT CCCCGCCGAG GGACATCACC GTCCACAGCG 
     2041 TGCTGGACTT CCTGCAGCTC GGCGCGCTGG CTGGCCTGCT CAGCCGTCTC ATCTGCCAGA 
     2101 GGCTCCAGGG TGGGAGCCCG CGGCAGGGCC CAGCAGCGGC TCCAGCCCTG GACACCCTCC 
     2161 CTGCCCCCAC CAGCCTGGTC CTGAGCCAGG TGACCTCCTC CAGCATCCGC CTGTCCTGGA 
     2221 CTCCAGCCCC CCGGCACCCC CTCAAGTATC TGATCGTTTG GCGAGCCTCT AGAGGTGGCA 
     2281 CCCCCAGGGA GGTGGTGGTG GAGGGACCCG CCGCCTCCAC GGAGCTGCAC AACCTGGCCT 
     2341 CCCGCACAGA GTACCTGGTC TCCGTGTTCC CCATCTATGA GGGCGGGGTT GGCGAAGGCC 
     2401 TGCGGGGCCT GGTGACCACA GCACCTCTGC CTCCGCCCCG GGCGCTGACC CTGGCCGCAG 
     2461 TGACGCCCAG AACCGTCCAC CTCACCTGGC AGCCCTCGGC CGGGGCCACC CACTACCTGG 
     2521 TGCGATGTTC TCCTGCTTCC CCCAAGGGTG AAGAGGAGGA GCGAGAGGTG CAGGTCGGGC 
     2581 GGCCCGAGGT GCTGCTGGAT GGCCTGGAAC CTGGCAGGGA CTATGAGGTC TCGGTGCAGA 
     2641 GCCTGCGAGG CCCTGAGGGC AGCGAGGCCC GGGGCATCCG TGCCAGGACC CCCACCCTGG 
     2701 CCCCCCCGAG ACACCTGGGC TTCTCAGACG TGAGCCACGA CGCGGCACGA GTGTTCTGGG 
     2761 AGGGTGCCCC GAGGCCTGTG CGCCTGGTCA GGGTCACCTA TGTGTCCAGC GAGGGTGGAC 
     2821 ACTCGGGGCA GACAGAGGCT CCTGGGAACG CCACCTCGGC CACGCTGGGG CCTCTCTCTT 
     2881 CCTCCACCAC CTACACTGTC CGTGTCACCT GCCTCTACCC TGGGGGTGGC TCCTCTACGC 
     2941 TGACTGGCCG GGTGACCACC AAGAAAGCTC CCAGCCCAAG CCAGCTGTCC ATGACGGAGC 
     3001 TGCCAGGGGA TGCAGTCCAG CTGGCGTGGG TGGCCGCAGC CCCGTCTGGC GTGCTTGTCT 
     3061 ACCAGATCAC GTGGACGCCC CTGGGAGAGG GGAAGGCTCA CGAGATCTCT GTCCCAGGGA 
     3121 ACCTCGGCAC GGCCGTCCTG CCTGGCCTAG GGAGGCACAC AGAGTACGAC GTCACCATCT 
     3181 TGGCCTACTA CAGGGACGGG GCCCGCAGTG ACCCTGTGTC CCTCCGCTAT ACCCCCTCCA 
     3241 CGGTGAGCAG GAGCCCACCC TCCAACCTGG CCCTGGCCTC GGAGACCCCC GACAGCCTGC 
     3301 AGGTCAGCTG GACGCCCCCG CTTGGCCGCG TGCTCCATTA CTGGCTCACC TACGCCCCCG 
     3361 CCTCTGGCTT GGGACCCGAG AAATCCGTCT CTGTGCCAGG AGCCAGGAGC CACGTGACAC 
     3421 TGCCCGACCT GCAGGCAGCC ACGAAGTACA GGGTCCTGGT CTCAGCTATC TATGCAGCAG 
     3481 GCAGGAGTGA GGCTGTGTCT GCCACGGGCC AGACAGCCTG CCCAGCCCTC CGCCCTGACG 
     3541 GCTCCCTCCC AGGGTTTGAC CTGATGGTGG CCTTCAGCCT GGTGGAAAAG GCTTATGCGT 
     3601 CCATCCGGGG CGTGGCCATG GAGCCCTCTG CCTTCGGTGG GACCCCGACC TTCACGCTCT 
     3661 TCAAGGACGC CCAGCTGACA AGACGGGTCA GTGACGTCTA CCCAGCCCCC CTACCTCCAG 
     3721 AGCACACCAT CGTCTTCCTT GTGCGCCTAC TTCCCGAGAC ACCCCGTGAG GCCTTCGCGC 
     3781 TGTGGCAGAT GACAGCCGAG GACTTCCAGC CCCTCCTTGG GGTTCTGCTG GATGCCGGGA 
     3841 AGAAGTCCCT GACCTACTTC CACCGTGACC CCAGGGCTGC CTTGCAGGAG GCCACCTTCG 
     3901 ACCCGCAGGA AGTGAGGAAG ATTTTCTTCG GGAGCTTCCA CAAGGTGCAC GTGGCTGTGG 
     3961 GCCGCTCCAA GGTCAGGCTC TATGTGGACT GCCGGAAGGT GGCTGAGCGG CCCCTTGGGG 
     4021 AGATGGGCAG CCCACCCGCT GCGGGCTTCG TCACGCTGGG GAGGCTGGCC AAGGCCAGGG 
     4081 GCCCCCGGAG CAGTTCGGCC GCGTTTCAGC TCCAGATGCT GCAGATCGTG TGCAGTGACA 
     4141 CCTGGGCCGA TGAGGACCGG TGCTGTGAGC TCCCTGCCTC GAGGGATGGA GAGACCTGCC 
     4201 CCGCCTTCGT GTCTGCCTGT TCCTGTTCCT CAGAGACCCC TGGGCCCCCA GGACCTCAAG 
     4261 GACCCCCAGG CCTCCCTGGG AGGAATGGCA CCCCAGGAGA GCAGGGCTTC CCAGGGCCCA 
     4321 GGGGTCCACC AGGGGTCAAA GGAGAGAAGG GAGACCATGG GCTTCCAGGC TTGCAGGGCC 
     4381 ACCCCGGCCA CCAGGGCATC CCCGGGAGAG TTGGCCTCCA GGGACCAAAG GGAATGAGAG 
     4441 GCCTGGAGGG AACTGCTGGC CTGCCTGGAC CCCCTGGCCC CAGGGGGTTC CAGGGCATGG 
     4501 CAGGGGCCAG GGGCACTAGT GGAGAGCGAG GACCTCCAGG GACCGTGGGG CCCACAGGAC 
     4561 TGCCAGGGCC CAAAGGGGAA CGAGGAGAGA AGGGCGAGCC GCAGTCCCTT GCCACCCTCT 
     4621 ACCAGCTTGT GAGCCAGGCC TCACACGTGT CAAAGTTCGA CTCCTTCCAC GAGAACACCA 
     4681 GGCCCCCCAT GCCCATCTTG GAGCAGAAGC TGGAGCCGGG CACTGAGCCC CTGGGGTCCC 
     4741 CTGGCACCCG CAGCAAGGCC CTGGTTCCTG GAGAATGGGG GCGTGGTGGC CGCCACCTTG 
     4801 AGGGCAGAGG GGAGCCTGGA GCTGTTGGTC AGATGGGCAG CCCTGGGCAG CAGGGGGCTA 
     4861 GCACCCAGGG CCTCTGGGAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4921 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4981 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5041 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5101 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5161 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5221 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5281 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5341 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5401 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5461 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5521 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5581 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5641 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5701 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5761 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5821 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5881 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5941 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6001 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6061 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6121 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6181 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6241 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6301 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6361 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6421 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6481 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6541 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6601 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6661 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6721 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6781 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6841 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6901 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6961 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7021 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7081 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7141 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7201 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7261 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7321 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7381 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7441 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7501 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7561 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7621 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7681 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7741 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7801 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7861 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7921 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7981 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8041 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8101 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8161 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8221 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8281 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8341 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8401 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8461 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8521 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8581 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8641 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8701 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//