LOCUS       RC207069                8175 bp    DNA     circular  SYN 
DEFINITION  MYO1F (Myc-DDK-tagged)-Human myosin IF (MYO1F)
ACCESSION   NM_012335
VERSION     NM_012335.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4332..4423 
                     /label="Myc-DDK"  
     misc_feature    4453..5038 
                     /label="PolyA signal"  
     misc_feature    4510..4529 
                     /label="Restriction Enzyme"  
     misc_feature    5314..5986 
                     /label="ColE1"  
     misc_feature    6416..7210 
                     /label="Kan/Neo"  
     misc_feature    7245..7547 
                     /label="SV40 ori"  
     misc_feature    7633..8110 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4314..4320 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4314 
                     /label="MYO1F"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAGCAAG GAGCGCTTCC ACTGGCAGAG CCACAACGTG AAGCAGAGCG 
     1081 GCGTGGATGA CATGGTGCTT CTTCCCCAGA TCACCGAAGA CGCCATTGCC GCCAACCTCC 
     1141 GGAAGCGCTT CATGGACGAC TACATCTTCA CCTACATCGG CTCTGTGCTC ATCTCTGTAA 
     1201 ACCCCTTCAA GCAGATGCCC TACTTCACCG ACCGTGAGAT CGACCTCTAT CAGGGCGCGG 
     1261 CCCAGTATGA GAATCCCCCG CACATCTACG CCCTCACGGA CAACATGTAC CGGAACATGC 
     1321 TTATCGACTG TGAGAACCAG TGTGTCATCA TTAGTGGAGA GAGTGGAGCT GGGAAGACAG 
     1381 TGGCAGCCAA ATATATCATG GGCTACATCT CCAAGGTGTC TGGCGGAGGC GAGAAGGTCC 
     1441 AGCACGTCAA AGATATCATC CTGCAGTCCA ACCCGCTGCT CGAGGCCTTC GGCAACGCCA 
     1501 AGACTGTGCG CAACAACAAT TCCAGCCGCT TTGGCAAGTA CTTTGAGATC CAGTTCAGCC 
     1561 GAGGTGGGGA GCCAGATGGG GGCAAGATCT CCAACTTCTT GCTGGAGAAG TCCCGCGTGG 
     1621 TCATGCAAAA TGAAAATGAG AGGAACTTCC ACATCTACTA CCAGCTGCTG GAAGGGGCCT 
     1681 CCCAGGAGCA AAGGCAGAAC CTGGGCCTCA TGACACCGGA CTACTATTAC TACCTCAACC 
     1741 AATCGGACAC CTACCAGGTG GACGGCACGG ACGACAGAAG CGACTTTGGT GAGACTCTGA 
     1801 GTGCTATGCA GGTTATTGGG ATCCCGCCCA GCATCCAGCA GCTGGTCCTG CAGCTCGTGG 
     1861 CGGGGATCTT GCACCTGGGG AACATCAGTT TCTGTGAAGA CGGGAATTAC GCCCGAGTGG 
     1921 AGAGTGTGGA CCTCCTGGCC TTTCCCGCCT ACCTGCTGGG CATTGACAGC GGGCGACTGC 
     1981 AGGAGAAGCT GACCAGCCGC AAGATGGACA GCCGCTGGGG CGGGCGCAGC GAGTCCATCA 
     2041 ATGTGACCCT CAACGTGGAG CAGGCAGCCT ACACCCGTGA TGCCCTGGCC AAGGGGCTCT 
     2101 ATGCCCGCCT CTTCGACTTC CTCGTGGAGG CCATCAACCG TGCTATGCAG AAACCCCAGG 
     2161 AAGAGTACAG CATCGGTGTG CTGGACATTT ACGGCTTCGA GATCTTCCAG AAAAATGGCT 
     2221 TCGAGCAGTT TTGCATCAAC TTCGTCAATG AGAAGCTGCA GCAAATCTTT ATCGAACTTA 
     2281 CCCTGAAGGC CGAGCAGGAG GAGTATGTGC AGGAAGGCAT CCGCTGGACT CCAATCCAGT 
     2341 ACTTCAACAA CAAGGTCGTC TGTGACCTCA TCGAAAACAA GCTGAGCCCC CCAGGCATCA 
     2401 TGAGCGTCTT GGACGACGTG TGCGCCACCA TGCACGCCAC GGGCGGGGGA GCAGACCAGA 
     2461 CACTGCTGCA GAAGCTGCAG GCGGCTGTGG GGACCCACGA GCATTTCAAC AGCTGGAGCG 
     2521 CCGGCTTCGT CATCCACCAC TACGCTGGCA AGGTCTCCTA CGACGTCAGC GGCTTCTGCG 
     2581 AGAGGAACCG AGACGTTCTC TTCTCCGACC TCATAGAGCT GATGCAGACC AGTGAGCAGG 
     2641 CCTTCCTCCG GATGCTCTTC CCCGAGAAGC TGGATGGAGA CAAGAAGGGG CGCCCCAGCA 
     2701 CCGCCGGCTC CAAGATCAAG AAACAAGCCA ACGACCTGGT GGCCACACTG ATGAGGTGCA 
     2761 CACCCCACTA CATCCGCTGC ATCAAACCCA ACGAGACCAA GAGGCCCCGA GACTGGGAGG 
     2821 AGAACAGAGT CAAGCACCAG GTGGAATACC TGGGCCTGAA GGAGAACATC AGGGTGCGCA 
     2881 GAGCCGGCTT CGCCTACCGC CGCCAGTTCG CCAAATTCCT GCAGAGGTAT GCCATTCTGA 
     2941 CCCCCGAGAC GTGGCCGCGG TGGCGTGGGG ACGAACGCCA GGGCGTCCAG CACCTGCTTC 
     3001 GGGCGGTCAA CATGGAGCCC GACCAGTACC AGATGGGGAG CACCAAGGTC TTTGTCAAGA 
     3061 ACCCAGAGTC GCTTTTCCTC CTGGAGGAGG TGCGAGAGCG AAAGTTCGAT GGCTTTGCCC 
     3121 GAACCATCCA GAAGGCCTGG CGGCGCCACG TGGCTGTCCG GAAGTACGAG GAGATGCGGG 
     3181 AGGAAGCTTC CAACATCCTG CTGAACAAGA AGGAGCGGAG GCGCAACAGC ATCAATCGGA 
     3241 ACTTCGTCGG GGACTACCTG GGGCTGGAGG AGCGGCCCGA GCTGCGTCAG TTCCTGGGCA 
     3301 AGAGGGAGCG GGTGGACTTC GCCGATTCGG TCACCAAGTA CGACCGCCGC TTCAAGCCCA 
     3361 TCAAGCGGGA CTTGATCCTG ACGCCCAAGT GTGTGTATGT GATTGGGCGA GAGAAAGTGA 
     3421 AGAAGGGACC TGAGAAGGGC CAGGTGTGTG AAGTCTTGAA GAAGAAAGTG GACATCCAGG 
     3481 CTCTGCGGGG AGTCTCCCTC AGCACGCGAC AGGACGACTT CTTCATCCTC CAAGAGGATG 
     3541 CCGCCGACAG CTTCCTGGAG AGCGTCTTCA AGACCGAGTT TGTCAGCCTT CTGTGCAAGC 
     3601 GCTTCGAGGA GGCGACGCGG AGGCCCCTGC CCCTCACCTT CAGCGACACA CTACAGTTTC 
     3661 GGGTGAAGAA GGAGGGCTGG GGCGGTGGCG GCACCCGCAG CGTCACCTTC TCCCGCGGCT 
     3721 TCGGCGACTT GGCAGTGCTC AAGGTTGGCG GTCGGACCCT CACGGTCAGC GTGGGCGATG 
     3781 GGCTGCCCAA GAGCTCCAAG CCTACGCGGA AGGGAATGGC CAAGGGAAAA CCTCGGAGGT 
     3841 CGTCCCAAGC CCCTACCCGG GCGGCCCCTG CGCCCCCCAG AGGCATGGAT CGCAATGGGG 
     3901 TGCCCCCCTC TGCCAGAGGG GGCCCCCTGC CCCTGGAGAT CATGTCTGGA GGGGGCACCC 
     3961 ACAGGCCTCC CCGGGGCCCT CCGTCCACAT CCCTGGGAGC CAGCAGACGA CCCCGGGCAC 
     4021 GTCCGCCCTC AGAGCACAAC ACAGAATTCC TCAACGTGCC TGACCAGGGC ATGGCCGGCA 
     4081 TGCAGAGGAA GCGCAGCGTG GGGCAACGGC CAGTGCCTGG TGTGGGCCGA CCCAAGCCCC 
     4141 AGCCTCGGAC ACATGGTCCC AGGTGCCGGG CCCTATACCA GTACGTGGGC CAAGATGTGG 
     4201 ACGAGCTGAG CTTCAACGTG AACGAGGTCA TTGAGATCCT CATGGAAGAT CCCTCGGGCT 
     4261 GGTGGAAGGG CCGGCTTCAC GGCCAGGAGG GCCTTTTCCC AGGAAACTAC GTGGAGAAGA 
     4321 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4381 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4441 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4501 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4561 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4621 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4681 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4741 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4801 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4861 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4921 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4981 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5041 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5101 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5161 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5221 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5281 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5341 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5401 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5461 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5521 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5581 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5641 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5701 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5761 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5821 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5881 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5941 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6001 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6061 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6121 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6181 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6241 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6301 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6361 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6421 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6481 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6541 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6601 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6661 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6721 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6781 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6841 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6901 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6961 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7021 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7081 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7141 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7201 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7261 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7321 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7381 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7441 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7501 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7561 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7621 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7681 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7741 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7801 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7861 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7921 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7981 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8041 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8101 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8161 TTTAACGCGA ATTTT
//