LOCUS       RC207021L1                9138 bp    DNA     circular  SYN 
DEFINITION  HK3 (Myc-DDK-tagged)-Human hexokinase 3 (white cell) (HK3), nuclear gene encoding mitochondrial protein
ACCESSION   NM_002115
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7427..7607 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8335..8994 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7766..8334 
                     /label="ColE1"  
     misc_feature    6302..6322 
                     /label="LR50 primer"  
     misc_feature    6099..6170 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6311..6902 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6089..6097 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6089 
                     /label="HK3"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGACTCCATT GGGTCTTCAG GGTTGCGGCA 
     3361 GGGGGAAGAA ACCCTGAGTT GCTCTGAGGA GGGCTTGCCC GGGCCCTCAG ACAGCTCAGA 
     3421 GCTGGTGCAG GAGTGCCTGC AGCAGTTCAA GGTGACAAGG GCACAGCTAC AGCAGATCCA 
     3481 AGCCAGCCTC TTGGGTTCCA TGGAGCAGGC GCTGAGGGGA CAGGCCAGCC CTGCCCCTGC 
     3541 GGTCCGGATG CTGCCTACAT ACGTGGGGTC CACCCCACAT GGCACTGAGC AAGGAGACTT 
     3601 CGTGGTGCTG GAGCTGGGGG CCACAGGGGC CTCACTGCGT GTTTTGTGGG TGACTCTAAC 
     3661 TGGCATTGAG GGGCATAGGG TGGAGCCCAG AAGCCAGGAG TTTGTGATCC CCCAAGAGGT 
     3721 GATGCTGGGT GCTGGCCAGC AGCTCTTTGA CTTTGCTGCC CACTGCCTGT CTGAGTTCCT 
     3781 GGATGCGCAG CCTGTGAACA AACAGGGTCT GCAGCTTGGC TTCAGCTTCT CTTTCCCTTG 
     3841 TCACCAGACG GGCTTGGACA GGAGCACCCT CATTTCCTGG ACCAAAGGTT TTAGGTGCAG 
     3901 TGGTGTGGAA GGCCAGGATG TGGTCCAGCT GCTGAGAGAT GCCATTCGGA GGCAGGGGGC 
     3961 CTACAACATC GACGTGGTTG CTGTGGTGAA CGACACAGTG GGCACCATGA TGGGCTGTGA 
     4021 GCCGGGGGTC AGGCCGTGTG AGGTTGGGCT AGTTGTAGAC ACGGGCACCA ACGCGTGTTA 
     4081 CATGGAGGAG GCACGGCATG TGGCAGTGCT GGACGAAGAC CGGGGCCGCG TCTGCGTCAG 
     4141 CGTCGAGTGG GGCTCCTTCA GCGATGATGG GGCGCTGGGA CCAGTGCTGA CCACCTTCGA 
     4201 CCATACCCTG GACCATGAGT CCCTGAATCC TGGTGCTCAG AGGTTTGAGA AGATGATCGG 
     4261 AGGCCTGTAC CTGGGTGAGC TGGTGCGGCT GGTGCTGGCT CACTTGGCCC GGTGTGGGGT 
     4321 CCTCTTTGGT GGCTGCACCT CCCCTGCCCT GCTGAGCCAA GGCAGCATCC TCCTGGAACA 
     4381 CGTGGCTGAG ATGGAGGACC CCTCTACTGG GGCAGCCCGT GTCCATGCTA TCCTGCAGGA 
     4441 CTTGGGCCTG AGCCCTGGGG CTTCGGATGT TGAGCTTGTG CAGCACGTCT GTGCGGCCGT 
     4501 GTGCACGCGG GCTGCCCAGC TCTGTGCTGC CGCCCTGGCC GCTGTTCTCT CCTGCCTCCA 
     4561 GCACAGCCGG GAGCAACAAA CACTCCAGGT TGCTGTGGCC ACCGGAGGCC GAGTGTGTGA 
     4621 GCGGCACCCC AGGTTCTGCA GCGTCCTGCA GGGGACAGTG ATGCTCCTGG CCCCGGAATG 
     4681 CGATGTCTCC TTAATCCCCT CTGTGGATGG TGGTGGCCGG GGAGTGGCGA TGGTGACTGC 
     4741 TGTGGCTGCC CGTCTGGCTG CCCACCGGCG CCTGCTGGAG GAGACCCTGG CCCCATTCCG 
     4801 GTTGAACCAT GATCAACTGG CTGCGGTTCA GGCACAGATG CGGAAGGCCA TGGCCAAGGG 
     4861 GCTCCGAGGG GAGGCCTCCT CCCTTCGCAT GCTGCCCACT TTCGTCCGGG CCACCCCTGA 
     4921 TGGCAGCGAG CGAGGGGATT TCCTGGCCCT GGACCTCGGG GGCACGAACT TCCGTGTCCT 
     4981 CCTGGTACGT GTGACCACAG GCGTGCAGAT CACCAGCGAG ATCTACTCCA TTCCCGAGAC 
     5041 TGTGGCCCAG GGTTCTGGGC AGCAGCTCTT TGACCACATC GTGGACTGCA TCGTGGACTT 
     5101 CCAGCAGAAG CAGGGCCTGA GCGGGCAGAG CCTCCCACTG GGTTTTACCT TCTCCTTCCC 
     5161 ATGTAGGCAG CTTGGCCTAG ACCAGGGCAT CCTCCTGAAC TGGACCAAGG GTTTCAAGGC 
     5221 ATCAGACTGC GAGGGCCAAG ATGTCGTGAG TCTGTTGCGG GAAGCCATCA CTCGCAGACA 
     5281 GGCAGTGGAG CTGAATGTGG TTGCCATTGT CAATGACACG GTGGGGACCA TGATGTCCTG 
     5341 TGGCTATGAG GACCCCCGTT GCGAGATAGG CCTCATTGTC GGAACCGGCA CCAATGCCTG 
     5401 CTACATGGAG GAGCTCCGGA ATGTGGCGGG CGTGCCTGGG GACTCAGGCC GCATGTGCAT 
     5461 CAACATGGAG TGGGGCGCCT TTGGGGACGA TGGCTCTCTG GCCATGCTCA GCACCCGCTT 
     5521 TGATGCAAGT GTGGACCAGG CGTCCATCAA CCCCGGCAAG CAGAGGTTTG AAAAGATGAT 
     5581 CAGCGGCATG TACCTGGGGG AGATCGTCCG CCACATCCTT TTACATTTAA CCAGCCTTGG 
     5641 CGTTCTCTTC CGGGGCCAGC AGATCCAGCG CCTTCAGACC AGGGACATCT TCAAGACCAA 
     5701 GTTCCTCTCT GAGATCGAAA GTGACAGCCT GGCCCTGCGG CAGGTCCGAG CCATCCTAGA 
     5761 GGATCTGGGG CTACCCCTGA CCTCAGATGA CGCCCTGATG GTGCTAGAGG TGTGCCAGGC 
     5821 TGTGTCCCAG AGGGCTGCCC AGCTCTGTGG GGCGGGTGTA GCTGCCGTGG TGGAGAAGAT 
     5881 CCGGGAGAAC CGGGGCCTGG AAGAGCTGGC AGTGTCTGTG GGGGTGGATG GAACGCTCTA 
     5941 CAAGCTGCAC CCGCGCTTCT CCAGCCTGGT GGCGGCCACA GTGCGGGAGC TGGCCCCTCG 
     6001 CTGTGTGGTC ACGTTCCTGC AGTCAGAGGA TGGGTCCGGC AAAGGTGCGG CCCTGGTCAC 
     6061 CGCTGTTGCC TGCCGCCTTG CGCAGTTGAC TCGTGTCACG CGGCCGCTCG AGCAGAAACT 
     6121 CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA 
     6181 GGTTTAAACG GCCGGCCGCG GTCTGTACAA GTAGGATTCG TCGAGGGACC TAATAACTTC 
     6241 GTATAGCATA CATTATACGA AGTTATACAT GTTTAAGGGT TCCGGTTCCA CTAGGTACAA 
     6301 TTCGATATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT 
     6361 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG 
     6421 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG 
     6481 CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG 
     6541 TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG 
     6601 ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC 
     6661 TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA 
     6721 TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC 
     6781 TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT 
     6841 CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC 
     6901 GCCTCCCCGC ATCGATACCG TCGACCTCGA TCGAGACCTA GAAAAACATG GAGCAATCAC 
     6961 AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA 
     7021 GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC 
     7081 TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA 
     7141 ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT TCCCTGATTG 
     7201 GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT GGTGCTACAA 
     7261 GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT GAAGGAGAGA ACACCCGCTT 
     7321 GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTAT TAGAGTGGAG 
     7381 GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG ACTGTACTGG 
     7441 GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT 
     7501 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG 
     7561 TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG 
     7621 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     7681 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     7741 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     7801 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     7861 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     7921 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     7981 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     8041 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     8101 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     8161 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     8221 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     8281 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     8341 CATGATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG 
     8401 CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC 
     8461 TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA 
     8521 TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC 
     8581 ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT 
     8641 TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA 
     8701 AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC 
     8761 AGCTCACCGT CTTTCATTGC CATACGGAAC TCCGGATGAG CATTCATCAG GCGGGCAAGA 
     8821 ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC 
     8881 GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA 
     8941 AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC 
     9001 TCCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     9061 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTCCCGCG CACATTTCCC 
     9121 CGAAAAGTGC CACCTGAC
//