LOCUS       RC207017                7923 bp    DNA     circular  SYN 
DEFINITION  RBL1 (Myc-DDK-tagged)-Human retinoblastoma-like 1 (p107) (RBL1), transcript variant 2
ACCESSION   NM_183404
VERSION     NM_183404.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4080..4171 
                     /label="Myc-DDK"  
     misc_feature    4201..4786 
                     /label="PolyA signal"  
     misc_feature    4258..4277 
                     /label="Restriction Enzyme"  
     misc_feature    5062..5734 
                     /label="ColE1"  
     misc_feature    6164..6958 
                     /label="Kan/Neo"  
     misc_feature    6993..7295 
                     /label="SV40 ori"  
     misc_feature    7381..7858 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4062..4068 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4062 
                     /label="RBL1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCGAGGAC AAGCCCCACG CTGAGGGGGC GGCGGTGGTC GCCGCAGCCG 
     1081 GGGAGGCGCT ACAGGCCCTG TGCCAGGAGC TGAACCTGGA CGAGGGGAGC GCGGCCGAAG 
     1141 CCCTGGACGA CTTTACTGCC ATCCGAGGCA ACTACAGCCT AGAGGGAGAA GTTACACACT 
     1201 GGTTGGCATG TTCATTATAT GTTGCATGCC GCAAAAGCAT TATTCCCACG GTTGGAAAGG 
     1261 GTATCATGGA AGGCAACTGT GTTTCACTTA CCAGAATACT ACGTTCAGCT AAATTAAGTT 
     1321 TAATACAATT TTTTAGTAAA ATGAAGAAAT GGATGGACAT GTCAAATCTA CCACAAGAAT 
     1381 TTCGTGAACG TATAGAAAGG CTAGAGAGAA ATTTTGAGGT GTCTACTGTA ATATTCAAAA 
     1441 AATATGAGCC AATTTTTTTA GATATATTTC AAAATCCATA TGAAGAACCA CCAAAGTTAC 
     1501 CACGAAGCCG GAAGCAGAGG AGGATTCCTT GCAGTGTTAA GGATCTGTTT AATTTCTGTT 
     1561 GGACACTTTT TGTTTATACT AAGGGTAATT TTCGGATGAT TGGGGATGAC TTAGTAAACT 
     1621 CTTATCATTT ACTTCTATGC TGCTTGGATC TGATTTTTGC CAATGCGATT ATGTGCCCAA 
     1681 ATAGACAAGA CTTGCTAAAT CCATCATTTA AAGGTTTACC ATCTGATTTT CATACTGCTG 
     1741 ACTTTACGGC TTCTGAAGAG CCACCCTGCA TCATTGCTGT ACTGTGTGAA CTGCATGATG 
     1801 GACTTCTCGT AGAAGCAAAA GGAATAAAGG AGCACTACTT TAAGCCATAT ATTTCAAAAC 
     1861 TCTTTGACAG GAAGATATTA AAAGGAGAAT GCCTCCTGGA CCTTTCAAGT TTTACTGATA 
     1921 ATAGCAAAGC AGTGAATAAG GAGTATGAAG AGTATGTTCT AACTGTTGGT GATTTTGATG 
     1981 AGAGGATCTT TTTGGGAGCA GACGCAGAAG AGGAAATTGG AACACCTCGA AAGTTCACTC 
     2041 GTGACACCCC ATTAGGGAAA CTGACAGCAC AGGCTAATGT GGAGTATAAC CTTCAACAGC 
     2101 ACTTTGAAAA AAAAAGGTCA TTTGCACCTT CTACCCCACT GACCGGACGG AGATATTTAC 
     2161 GAGAAAAAGA AGCAGTCATT ACTCCTGTTG CATCAGCCAC CCAAAGTGTG AGCCGGTTAC 
     2221 AGAGTATTGT GGCTGGTCTG AAAAATGCAC CAAGTGACCA ACTTATAAAT ATTTTTGAAT 
     2281 CTTGTGTGCG TAATCCTGTG GAAAACATTA TGAAAATACT AAAAGGAATA GGAGAGACTT 
     2341 TCTGTCAACA CTATACTCAA TCAACAGATG AACAGCCAGG ATCTCACATA GACTTTGCTG 
     2401 TAAACAGACT AAAGCTGGCA GAAATTTTGT ATTATAAAAT ACTAGAGACT GTAATGGTTC 
     2461 AGGAAACACG AAGACTTCAT GGAATGGACA TGTCAGTTCT TTTAGAGCAA GATATATTTC 
     2521 ATCGTTCCTT GATGGCTTGT TGTTTGGAAA TTGTGCTCTT TGCCTATAGC TCACCTCGTA 
     2581 CTTTTCCTTG GATTATTGAA GTTCTCAACT TGCAACCATT TTACTTTTAT AAGGTTATTG 
     2641 AGGTGGTGAT CCGCTCAGAA GAGGGGCTCT CAAGGGACAT GGTGAAACAC CTAAACAGCA 
     2701 TTGAAGAACA GATTTTGGAG AGTTTAGCAT GGAGTCACGA TTCTGCACTG TGGGAGGCTC 
     2761 TCCAGGTTTC TGCAAACAAA GTTCCTACCT GTGAAGAAGT TATATTCCCA AATAACTTTG 
     2821 AAACAGGAAA TGGAGGAAAT GTGCAGGGAC ATCTTCCCCT GATGCCAATG TCTCCTCTAA 
     2881 TGCACCCAAG AGTCAAGGAA GTTCGAACTG ACAGTGGGAG TCTTCGAAGA GATATGCAAC 
     2941 CATTGTCTCC AATTTCTGTC CATGAACGCT ACAGTTCTCC TACCGCAGGG AGTGCTAAGA 
     3001 GAAGACTCTT TGGAGAGGAC CCCCCAAAGG AAATGCTTAT GGACAAGATC ATAACAGAAG 
     3061 GAACAAAATT GAAAATCGCT CCTTCTTCAA GCATTACTGC TGAAAATGTA TCAATTTTAC 
     3121 CTGGTCAAAC TCTTCTAACA ATGGCCACAG CCCCAGTAAC AGGAACAACA GGACATAAAG 
     3181 TTACAATTCC ATTACATGGT GTCGCAAATG ATGCTGGAGA GATCACACTG ATACCTCTTT 
     3241 CCATGAATAC AAATCAGGAG TCCAAAGTCA AGAGTCCTGT ATCACTTACT GCTCATTCAT 
     3301 TAATTGGTGC TTCTCCAAAA CAGACCAATC TGACTAAAGC ACAAGAGGTA CATTCAACTG 
     3361 GAATAAACAG GCCAAAGAGA ACTGGGTCCT TAGCACTATT TTACAGAAAG GTCTATCATT 
     3421 TGGCAAGTGT ACGCTTACGT GATCTATGTC TAAAACTGGA TGTTTCAAAT GAGTTACGAA 
     3481 GGAAGATATG GACGTGTTTT GAATTCACTT TAGTTCACTG TCCTGATCTA ATGAAAGACA 
     3541 GGCATTTGGA TCAGCTCCTC CTTTGTGCCT TTTATATCAT GGCAAAGGTA ACAAAAGAAG 
     3601 AAAGAACTTT TCAAGAAATT ATGAAAAGTT ATAGGAATCA GCCCCAAGCT AATAGTCACG 
     3661 TATATAGAAG TGTTCTGCTG AAAAGTATTC CAAGAGAAGT TGTGGCATAT AATAAAAATA 
     3721 TAAATGATGA CTTTGAAATG ATAGATTGTG ACTTAGAAGA TGCTACAAAA ACACCTGACT 
     3781 GTTCCAGTGG ACCAGTGAAA GAGGAAAGAG GTGATCTTAT AAAATTTTAC AATACAATAT 
     3841 ATGTAGGAAG AGTGAAGTCA TTTGCACTGA AATACGACTT GGCGAATCAG GACCATATGA 
     3901 TGGATGCTCC ACCACTCTCT CCTTTTCCAC ATATTAAACA ACAGCCAGGC TCACCACGCC 
     3961 GCATTTCCCA GCAGCACTCC ATTTATATTT CCCCGCACAA GAATGGGTCA GGCCTTACAC 
     4021 CAAGAAGCGC TCTGCTGTAC AAGTTCAATG GCAGCCCTTC TAAGGTAAGG ACGCGTACGC 
     4081 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4141 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4201 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4261 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4321 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4381 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4441 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4501 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4561 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4621 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4681 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4741 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4801 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4861 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4921 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4981 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5041 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5101 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5161 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5221 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5281 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5341 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5401 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5461 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5521 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5581 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5641 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5701 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5761 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5821 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5881 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5941 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6001 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6061 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6121 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6181 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6241 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6301 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6361 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6421 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6481 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6541 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6601 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6661 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6721 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6781 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6841 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6901 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6961 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7021 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7081 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7141 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7201 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7261 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7321 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7381 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7441 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7501 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7561 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7621 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7681 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7741 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7801 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7861 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7921 TTT
//