LOCUS RC206970L4 9455 bp DNA circular SYN DEFINITION PINK1 (Myc-DDK-tagged)-Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein ACCESSION NM_032409 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 7746..7926 /label="3-LTR" misc_feature 833..1013 /label="5-LTR" misc_feature 8654..9300 /label="CAT(Cam-r)" misc_feature 2620..3234 /label="CMV" misc_feature 6621..6641 /label="LR50" misc_feature 5084..5812 /label="mGFP" misc_feature 5819..5884 /label="P2A" misc_feature 7974..8593 /label="pBR322 Ori" misc_feature 5885..6484 /label="Puro" misc_feature 3252..3271 /label="T7" misc_feature 3221..3237 /label="V2" misc_feature 3320..3328 /label="Restriction Enzyme" misc_feature 5063..5069 /label="Restriction Enzyme" misc_feature 3320..5063 /label="PINK1" ORIGIN 1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCGGTGCGA CAGGCGCTGG GCCGCGGCCT 3361 GCAGCTGGGT CGAGCGCTGC TGCTGCGCTT CACGGGCAAG CCCGGCCGGG CCTACGGCTT 3421 GGGGCGGCCG GGCCCGGCGG CGGGCTGTGT CCGCGGGGAG CGTCCAGGCT GGGCCGCAGG 3481 ACCGGGCGCG GAGCCTCGCA GGGTCGGGCT CGGGCTCCCT AACCGTCTCC GCTTCTTCCG 3541 CCAGTCGGTG GCCGGGCTGG CGGCGCGGTT GCAGCGGCAG TTCGTGGTGC GGGCCTGGGG 3601 CTGCGCGGGC CCTTGCGGCC GGGCAGTCTT TCTGGCCTTC GGGCTAGGGC TGGGCCTCAT 3661 CGAGGAAAAA CAGGCGGAGA GCCGGCGGGC GGTCTCGGCC TGTCAGGAGA TCCAGGCAAT 3721 TTTTACCCAG AAAAGCAAGC CGGGGCCTGA CCCGTTGGAC ACGAGACGCT TGCAGGGCTT 3781 TCGGCTGGAG GAGTATCTGA TAGGGCAGTC CATTGGTAAG GGCTGCAGTG CTGCTGTGTA 3841 TGAAGCCACC ATGCCTACAT TGCCCCAGAA CCTGGAGGTG ACAAAGAGCA CCGGGTTGCT 3901 TCCAGGGAGA GGCCCAGGTA CCAGTGCACC AGGAGAAGGG CAGGAGCGAG CTGCGGGGGC 3961 CCCTGCCTTC CCCTTGGCCA TCAAGATGAT GTGGAACATC TCGGCAGGTT CCTCCAGCGA 4021 AGCCATCTTG AACACAATGA GCCAGGAGCT GGTCCCAGCG AGCCGAGTGG CCTTGGCTGG 4081 GGAGTATGGA GCAGTCACTT ACAGAAAATC CAAGAGAGGT CCCAAGCAAC TAGCCCCTCA 4141 CCCCAACATC ATCCGGGTTC TCCGCGCCTT CACCTCTTCC GTGCCGCTGC TGCCAGGGGC 4201 CCTGGTCGAC TACCCTGATG TGCTGCCCTC ACGCCTCCAC CCTGAAGGCC TGGGCCATGG 4261 CCGGACGCTG TTCCTCGTTA TGAAGAACTA TCCCTGTACC CTGCGCCAGT ACCTTTGTGT 4321 GAACACACCC AGCCCCCGCC TCGCCGCCAT GATGCTGCTG CAGCTGCTGG AAGGCGTGGA 4381 CCATCTGGTT CAACAGGGCA TCGCGCACAG AGACCTGAAA TCCGACAACA TCCTTGTGGA 4441 GCTGGACCCA GACGGCTGCC CCTGGCTGGT GATCGCAGAT TTTGGCTGCT GCCTGGCTGA 4501 TGAGAGCATC GGCCTGCAGT TGCCCTTCAG CAGCTGGTAC GTGGATCGGG GCGGAAACGG 4561 CTGTCTGATG GCCCCAGAGG TGTCCACGGC CCGTCCTGGC CCCAGGGCAG TGATTGACTA 4621 CAGCAAGGCT GATGCCTGGG CAGTGGGAGC CATCGCCTAT GAAATCTTCG GGCTTGTCAA 4681 TCCCTTCTAC GGCCAGGGCA AGGCCCACCT TGAAAGCCGC AGCTACCAAG AGGCTCAGCT 4741 ACCTGCACTG CCCGAGTCAG TGCCTCCAGA CGTGAGACAG TTGGTGAGGG CACTGCTCCA 4801 GCGAGAGGCC AGCAAGAGAC CATCTGCCCG AGTAGCCGCA AATGTGCTTC ATCTAAGCCT 4861 CTGGGGTGAA CATATTCTAG CCCTGAAGAA TCTGAAGTTA GACAAGATGG TTGGCTGGCT 4921 CCTCCAACAA TCGGCCGCCA CTTTGTTGGC CAACAGGCTC ACAGAGAAGT GTTGTGTGGA 4981 AACAAAAATG AAGATGCTCT TTCTGGCTAA CCTGGAGTGT GAAACGCTCT GCCAGGCAGC 5041 CATCCTCCTC TGCTCATGGA GGGCAGCCCT GACGCGTACG CGGCCGCTCG AGATGAGCGG 5101 GGGCGAGGAG CTGTTCGCCG GCATCGTGCC CGTGCTGATC GAGCTGGACG GCGACGTGCA 5161 CGGCCACAAG TTCAGCGTGC GCGGCGAGGG CGAGGGCGAC GCCGACTACG GCAAGCTGGA 5221 GATCAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TGGTGACCAC 5281 CCTCTGCTAC GGCATCCAGT GCTTCGCCCG CTACCCCGAG CACATGAAGA TGAACGACTT 5341 CTTCAAGAGC GCCATGCCCG AGGGCTACAT CCAGGAGCGC ACCATCCAGT TCCAGGACGA 5401 CGGCAAGTAC AAGACCCGCG GCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT 5461 CGAGCTGAAG GGCAAGGACT TCAAGGAGGA CGGCAACATC CTGGGCCACA AGCTGGAGTA 5521 CAGCTTCAAC AGCCACAACG TGTACATCCG CCCCGACAAG GCCAACAACG GCCTGGAGGC 5581 TAACTTCAAG ACCCGCCACA ACATCGAGGG CGGCGGCGTG CAGCTGGCCG ACCACTACCA 5641 GACCAACGTG CCCCTGGGCG ACGGCCCCGT GCTGATCCCC ATCAACCACT ACCTGAGCAC 5701 TCAGACCAAG ATCAGCAAGG ACCGCAACGA GGCCCGCGAC CACATGGTGC TCCTGGAGTC 5761 CTTCAGCGCC TGCTGCCACA CCCACGGCAT GGACGAGCTG TACAGGTCCG GACTCAGAGT 5821 TTGGGTAGGA AGCGGAGCTA CTAACTTCAG CCTGCTGAAG CAGGCTGGAG ACGTGGAGGA 5881 GAACCCTGGA CCTATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT 5941 CCCCAGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC 6001 CGTCGATCCG GATCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG 6061 CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG 6121 GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC 6181 CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA 6241 CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG 6301 CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT 6361 GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT 6421 CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA 6481 GCCCGGTGCC TGATGTACAC CCAAACGGCC GGCCGCGGTC TGTACAAGTA GGATTCGTCG 6541 AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATACATGTT TAAGGGTTCC 6601 GGTTCCACTA GGTACAATTC GATATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA 6661 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG 6721 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT 6781 TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG 6841 GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT 6901 GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG 6961 CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG 7021 TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC 7081 TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC 7141 GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC 7201 GGATCTCCCT TTGGGCCGCC TCCCCGCATC GATACCGTCG ACCTCGATCG AGACCTAGAA 7261 AAACATGGAG CAATCACAAG TAGCAATACA GCAGCTACCA ATGCTGATTG TGCCTGGCTA 7321 GAAGCACAAG AGGAGGAGGA GGTGGGTTTT CCAGTCACAC CTCAGGTACC TTTAAGACCA 7381 ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA 7441 GGGCTAATTC ACTCCCAACG AAGACAAGAT ATCCTTGATC TGTGGATCTA CCACACACAA 7501 GGCTACTTCC CTGATTGGCA GAACTACACA CCAGGGCCAG GGATCAGATA TCCACTGACC 7561 TTTGGATGGT GCTACAAGCT AGTACCAGTT GAGCAAGAGA AGGTAGAAGA AGCCAATGAA 7621 GGAGAGAACA CCCGCTTGTT ACACCCTGTG AGCCTGCATG GGATGGATGA CCCGGAGAGA 7681 GAAGTATTAG AGTGGAGGTT TGACAGCCGC CTAGCATTTC ATCACATGGC CCGAGAGCTG 7741 CATCCGGACT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC 7801 TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG 7861 TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG 7921 TGGAAAATCT CTAGCAGCAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 7981 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 8041 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 8101 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 8161 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG 8221 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 8281 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 8341 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 8401 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT 8461 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 8521 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 8581 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 8641 CGTTAAGGGA TTTTGGTCAT GATTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA 8701 ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG 8761 CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATGGTG AAAACGGGGG 8821 CGAAGAAGTT GTCCATATTG GCCACGTTTA AATCAAAACT GGTGAAACTC ACCCAGGGAT 8881 TGGCTGAGAC GAAAAACATA TTCTCAATAA ACCCTTTAGG GAAATAGGCC AGGTTTTCAC 8941 CGTAACACGC CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG TCGTGGTATT 9001 CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA AACGGTGTAA CAAGGGTGAA 9061 CACTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT ACGGAACTCC GGATGAGCAT 9121 TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA TTTTTCTTTA 9181 CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA CATTGAGCAA 9241 CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT 9301 ATCCAGTGAT TTTTTTCTCC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 9361 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 9421 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGAC //