LOCUS RC206970L1 8118 bp DNA circular SYN DEFINITION PINK1 (Myc-DDK-tagged)-Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein ACCESSION NM_032409 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 6409..6589 /label="3'LTR" misc_feature 833..1013 /label="5'LTR" misc_feature 7317..7976 /label="CAT(Cam-r)" misc_feature 2620..3234 /label="CMV promoter" misc_feature 6748..7316 /label="ColE1" misc_feature 5284..5304 /label="LR50 primer" misc_feature 5081..5152 /label="Myc-DDK" misc_feature 3252..3271 /label="T7 promoter" misc_feature 3221..3237 /label="V2 primer" misc_feature 5293..5884 /label="WPRE" misc_feature 3320..3328 /label="Restriction Enzyme" misc_feature 5063..5069 /label="Restriction Enzyme" misc_feature 3320..5063 /label="PINK1" ORIGIN 1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCGGTGCGA CAGGCGCTGG GCCGCGGCCT 3361 GCAGCTGGGT CGAGCGCTGC TGCTGCGCTT CACGGGCAAG CCCGGCCGGG CCTACGGCTT 3421 GGGGCGGCCG GGCCCGGCGG CGGGCTGTGT CCGCGGGGAG CGTCCAGGCT GGGCCGCAGG 3481 ACCGGGCGCG GAGCCTCGCA GGGTCGGGCT CGGGCTCCCT AACCGTCTCC GCTTCTTCCG 3541 CCAGTCGGTG GCCGGGCTGG CGGCGCGGTT GCAGCGGCAG TTCGTGGTGC GGGCCTGGGG 3601 CTGCGCGGGC CCTTGCGGCC GGGCAGTCTT TCTGGCCTTC GGGCTAGGGC TGGGCCTCAT 3661 CGAGGAAAAA CAGGCGGAGA GCCGGCGGGC GGTCTCGGCC TGTCAGGAGA TCCAGGCAAT 3721 TTTTACCCAG AAAAGCAAGC CGGGGCCTGA CCCGTTGGAC ACGAGACGCT TGCAGGGCTT 3781 TCGGCTGGAG GAGTATCTGA TAGGGCAGTC CATTGGTAAG GGCTGCAGTG CTGCTGTGTA 3841 TGAAGCCACC ATGCCTACAT TGCCCCAGAA CCTGGAGGTG ACAAAGAGCA CCGGGTTGCT 3901 TCCAGGGAGA GGCCCAGGTA CCAGTGCACC AGGAGAAGGG CAGGAGCGAG CTGCGGGGGC 3961 CCCTGCCTTC CCCTTGGCCA TCAAGATGAT GTGGAACATC TCGGCAGGTT CCTCCAGCGA 4021 AGCCATCTTG AACACAATGA GCCAGGAGCT GGTCCCAGCG AGCCGAGTGG CCTTGGCTGG 4081 GGAGTATGGA GCAGTCACTT ACAGAAAATC CAAGAGAGGT CCCAAGCAAC TAGCCCCTCA 4141 CCCCAACATC ATCCGGGTTC TCCGCGCCTT CACCTCTTCC GTGCCGCTGC TGCCAGGGGC 4201 CCTGGTCGAC TACCCTGATG TGCTGCCCTC ACGCCTCCAC CCTGAAGGCC TGGGCCATGG 4261 CCGGACGCTG TTCCTCGTTA TGAAGAACTA TCCCTGTACC CTGCGCCAGT ACCTTTGTGT 4321 GAACACACCC AGCCCCCGCC TCGCCGCCAT GATGCTGCTG CAGCTGCTGG AAGGCGTGGA 4381 CCATCTGGTT CAACAGGGCA TCGCGCACAG AGACCTGAAA TCCGACAACA TCCTTGTGGA 4441 GCTGGACCCA GACGGCTGCC CCTGGCTGGT GATCGCAGAT TTTGGCTGCT GCCTGGCTGA 4501 TGAGAGCATC GGCCTGCAGT TGCCCTTCAG CAGCTGGTAC GTGGATCGGG GCGGAAACGG 4561 CTGTCTGATG GCCCCAGAGG TGTCCACGGC CCGTCCTGGC CCCAGGGCAG TGATTGACTA 4621 CAGCAAGGCT GATGCCTGGG CAGTGGGAGC CATCGCCTAT GAAATCTTCG GGCTTGTCAA 4681 TCCCTTCTAC GGCCAGGGCA AGGCCCACCT TGAAAGCCGC AGCTACCAAG AGGCTCAGCT 4741 ACCTGCACTG CCCGAGTCAG TGCCTCCAGA CGTGAGACAG TTGGTGAGGG CACTGCTCCA 4801 GCGAGAGGCC AGCAAGAGAC CATCTGCCCG AGTAGCCGCA AATGTGCTTC ATCTAAGCCT 4861 CTGGGGTGAA CATATTCTAG CCCTGAAGAA TCTGAAGTTA GACAAGATGG TTGGCTGGCT 4921 CCTCCAACAA TCGGCCGCCA CTTTGTTGGC CAACAGGCTC ACAGAGAAGT GTTGTGTGGA 4981 AACAAAAATG AAGATGCTCT TTCTGGCTAA CCTGGAGTGT GAAACGCTCT GCCAGGCAGC 5041 CATCCTCCTC TGCTCATGGA GGGCAGCCCT GACGCGTACG CGGCCGCTCG AGCAGAAACT 5101 CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA 5161 GGTTTAAACG GCCGGCCGCG GTCTGTACAA GTAGGATTCG TCGAGGGACC TAATAACTTC 5221 GTATAGCATA CATTATACGA AGTTATACAT GTTTAAGGGT TCCGGTTCCA CTAGGTACAA 5281 TTCGATATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT 5341 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG 5401 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG 5461 CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG 5521 TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG 5581 ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC 5641 TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA 5701 TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC 5761 TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT 5821 CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC 5881 GCCTCCCCGC ATCGATACCG TCGACCTCGA TCGAGACCTA GAAAAACATG GAGCAATCAC 5941 AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA 6001 GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC 6061 TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA 6121 ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT TCCCTGATTG 6181 GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT GGTGCTACAA 6241 GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT GAAGGAGAGA ACACCCGCTT 6301 GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTAT TAGAGTGGAG 6361 GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG ACTGTACTGG 6421 GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT 6481 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG 6541 TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG 6601 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 6661 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 6721 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 6781 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 6841 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 6901 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 6961 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 7021 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 7081 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 7141 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 7201 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 7261 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 7321 CATGATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG 7381 CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC 7441 TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA 7501 TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC 7561 ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT 7621 TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA 7681 AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC 7741 AGCTCACCGT CTTTCATTGC CATACGGAAC TCCGGATGAG CATTCATCAG GCGGGCAAGA 7801 ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC 7861 GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA 7921 AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC 7981 TCCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 8041 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTCCCGCG CACATTTCCC 8101 CGAAAAGTGC CACCTGAC //