LOCUS RC206970 6624 bp DNA circular SYN DEFINITION PINK1 (Myc-DDK-tagged)-Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein ACCESSION NM_032409 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 2781..2872 /label="Myc-DDK" misc_feature 2902..3487 /label="PolyA signal" misc_feature 2959..2978 /label="Restriction Enzyme" misc_feature 3763..4435 /label="ColE1" misc_feature 4865..5659 /label="Kan/Neo" misc_feature 5694..5996 /label="SV40 ori" misc_feature 6082..6559 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 2763..2769 /label="Restriction Enzyme" misc_feature 1020..2763 /label="PINK1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGCGGTGCGA CAGGCGCTGG GCCGCGGCCT GCAGCTGGGT CGAGCGCTGC 1081 TGCTGCGCTT CACGGGCAAG CCCGGCCGGG CCTACGGCTT GGGGCGGCCG GGCCCGGCGG 1141 CGGGCTGTGT CCGCGGGGAG CGTCCAGGCT GGGCCGCAGG ACCGGGCGCG GAGCCTCGCA 1201 GGGTCGGGCT CGGGCTCCCT AACCGTCTCC GCTTCTTCCG CCAGTCGGTG GCCGGGCTGG 1261 CGGCGCGGTT GCAGCGGCAG TTCGTGGTGC GGGCCTGGGG CTGCGCGGGC CCTTGCGGCC 1321 GGGCAGTCTT TCTGGCCTTC GGGCTAGGGC TGGGCCTCAT CGAGGAAAAA CAGGCGGAGA 1381 GCCGGCGGGC GGTCTCGGCC TGTCAGGAGA TCCAGGCAAT TTTTACCCAG AAAAGCAAGC 1441 CGGGGCCTGA CCCGTTGGAC ACGAGACGCT TGCAGGGCTT TCGGCTGGAG GAGTATCTGA 1501 TAGGGCAGTC CATTGGTAAG GGCTGCAGTG CTGCTGTGTA TGAAGCCACC ATGCCTACAT 1561 TGCCCCAGAA CCTGGAGGTG ACAAAGAGCA CCGGGTTGCT TCCAGGGAGA GGCCCAGGTA 1621 CCAGTGCACC AGGAGAAGGG CAGGAGCGAG CTGCGGGGGC CCCTGCCTTC CCCTTGGCCA 1681 TCAAGATGAT GTGGAACATC TCGGCAGGTT CCTCCAGCGA AGCCATCTTG AACACAATGA 1741 GCCAGGAGCT GGTCCCAGCG AGCCGAGTGG CCTTGGCTGG GGAGTATGGA GCAGTCACTT 1801 ACAGAAAATC CAAGAGAGGT CCCAAGCAAC TAGCCCCTCA CCCCAACATC ATCCGGGTTC 1861 TCCGCGCCTT CACCTCTTCC GTGCCGCTGC TGCCAGGGGC CCTGGTCGAC TACCCTGATG 1921 TGCTGCCCTC ACGCCTCCAC CCTGAAGGCC TGGGCCATGG CCGGACGCTG TTCCTCGTTA 1981 TGAAGAACTA TCCCTGTACC CTGCGCCAGT ACCTTTGTGT GAACACACCC AGCCCCCGCC 2041 TCGCCGCCAT GATGCTGCTG CAGCTGCTGG AAGGCGTGGA CCATCTGGTT CAACAGGGCA 2101 TCGCGCACAG AGACCTGAAA TCCGACAACA TCCTTGTGGA GCTGGACCCA GACGGCTGCC 2161 CCTGGCTGGT GATCGCAGAT TTTGGCTGCT GCCTGGCTGA TGAGAGCATC GGCCTGCAGT 2221 TGCCCTTCAG CAGCTGGTAC GTGGATCGGG GCGGAAACGG CTGTCTGATG GCCCCAGAGG 2281 TGTCCACGGC CCGTCCTGGC CCCAGGGCAG TGATTGACTA CAGCAAGGCT GATGCCTGGG 2341 CAGTGGGAGC CATCGCCTAT GAAATCTTCG GGCTTGTCAA TCCCTTCTAC GGCCAGGGCA 2401 AGGCCCACCT TGAAAGCCGC AGCTACCAAG AGGCTCAGCT ACCTGCACTG CCCGAGTCAG 2461 TGCCTCCAGA CGTGAGACAG TTGGTGAGGG CACTGCTCCA GCGAGAGGCC AGCAAGAGAC 2521 CATCTGCCCG AGTAGCCGCA AATGTGCTTC ATCTAAGCCT CTGGGGTGAA CATATTCTAG 2581 CCCTGAAGAA TCTGAAGTTA GACAAGATGG TTGGCTGGCT CCTCCAACAA TCGGCCGCCA 2641 CTTTGTTGGC CAACAGGCTC ACAGAGAAGT GTTGTGTGGA AACAAAAATG AAGATGCTCT 2701 TTCTGGCTAA CCTGGAGTGT GAAACGCTCT GCCAGGCAGC CATCCTCCTC TGCTCATGGA 2761 GGGCAGCCCT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 2821 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 2881 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 2941 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 3001 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 3061 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 3121 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 3181 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 3241 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 3301 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 3361 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 3421 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 3481 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 3541 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 3601 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 3661 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 3721 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 3781 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 3841 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 3901 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 3961 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 4021 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 4081 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 4141 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 4201 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 4261 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 4321 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 4381 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 4441 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 4501 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 4561 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 4621 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 4681 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 4741 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 4801 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 4861 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 4921 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 4981 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 5041 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 5101 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 5161 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 5221 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 5281 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 5341 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 5401 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 5461 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 5521 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 5581 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 5641 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 5701 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 5761 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 5821 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 5881 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 5941 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 6001 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 6061 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 6121 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 6181 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 6241 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 6301 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 6361 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 6421 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 6481 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 6541 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 6601 TAACAAAAAT TTAACGCGAA TTTT //