LOCUS       RC206933                7674 bp    DNA     circular  SYN 
DEFINITION  RB1 (Myc-DDK-tagged)-Human retinoblastoma 1 (RB1)
ACCESSION   NM_000321
VERSION     NM_000321.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3829..3920 
                     /label="Myc-DDK"  
     misc_feature    3950..4535 
                     /label="PolyA signal"  
     misc_feature    4007..4026 
                     /label="Restriction Enzyme"  
     misc_feature    4811..5483 
                     /label="ColE1"  
     misc_feature    5913..6707 
                     /label="Kan/Neo"  
     misc_feature    6742..7044 
                     /label="SV40 ori"  
     misc_feature    7130..7607 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3804..3811 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3804 
                     /label="RB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGCCCAAA ACCCCCCGAA AAACGGCCGC CACCGCCGCC GCTGCCGCCG 
     1081 CGGAACCCCC GGCACCGCCG CCGCCGCCCC CTCCTGAGGA GGACCCAGAG CAGGACAGCG 
     1141 GCCCGGAGGA CCTGCCTCTC GTCAGGCTTG AGTTTGAAGA AACAGAAGAA CCTGATTTTA 
     1201 CTGCATTATG TCAGAAATTA AAGATACCAG ATCATGTCAG AGAGAGAGCT TGGTTAACTT 
     1261 GGGAGAAAGT TTCATCTGTG GATGGAGTAT TGGGAGGTTA TATTCAAAAG AAAAAGGAAC 
     1321 TGTGGGGAAT CTGTATCTTT ATTGCAGCAG TTGACCTAGA TGAGATGTCG TTCACTTTTA 
     1381 CTGAGCTACA GAAAAACATA GAAATCAGTG TCCATAAATT CTTTAACTTA CTAAAAGAAA 
     1441 TTGATACCAG TACCAAAGTT GATAATGCTA TGTCAAGACT GTTGAAGAAG TATGATGTAT 
     1501 TGTTTGCACT CTTCAGCAAA TTGGAAAGGA CATGTGAACT TATATATTTG ACACAACCCA 
     1561 GCAGTTCGAT ATCTACTGAA ATAAATTCTG CATTGGTGCT AAAAGTTTCT TGGATCACAT 
     1621 TTTTATTAGC TAAAGGGGAA GTATTACAAA TGGAAGATGA TCTGGTGATT TCATTTCAGT 
     1681 TAATGCTATG TGTCCTTGAC TATTTTATTA AACTCTCACC TCCCATGTTG CTCAAAGAAC 
     1741 CATATAAAAC AGCTGTTATA CCCATTAATG GTTCACCTCG AACACCCAGG CGAGGTCAGA 
     1801 ACAGGAGTGC ACGGATAGCA AAACAACTAG AAAATGATAC AAGAATTATT GAAGTTCTCT 
     1861 GTAAAGAACA TGAATGTAAT ATAGATGAGG TGAAAAATGT TTATTTCAAA AATTTTATAC 
     1921 CTTTTATGAA TTCTCTTGGA CTTGTAACAT CTAATGGACT TCCAGAGGTT GAAAATCTTT 
     1981 CTAAACGATA CGAAGAAATT TATCTTAAAA ATAAAGATCT AGATGCAAGA TTATTTTTGG 
     2041 ATCATGATAA AACTCTTCAG ACTGATTCTA TAGACAGTTT TGAAACACAG AGAACACCAC 
     2101 GAAAAAGTAA CCTTGATGAA GAGGTGAATG TAATTCCTCC ACACACTCCA GTTAGGACTG 
     2161 TTATGAACAC TATCCAACAA TTAATGATGA TTTTAAATTC AGCAAGTGAT CAACCTTCAG 
     2221 AAAATCTGAT TTCCTATTTT AACAACTGCA CAGTGAATCC AAAAGAAAGT ATACTGAAAA 
     2281 GAGTGAAGGA TATAGGATAC ATCTTTAAAG AGAAATTTGC TAAAGCTGTG GGACAGGGTT 
     2341 GTGTCGAAAT TGGATCACAG CGATACAAAC TTGGAGTTCG CTTGTATTAC CGAGTAATGG 
     2401 AATCCATGCT TAAATCAGAA GAAGAACGAT TATCCATTCA AAATTTTAGC AAACTTCTGA 
     2461 ATGACAACAT TTTTCATATG TCTTTATTGG CGTGCGCTCT TGAGGTTGTA ATGGCCACAT 
     2521 ATAGCAGAAG TACATCTCAG AATCTTGATT CTGGAACAGA TTTGTCTTTC CCATGGATTC 
     2581 TGAATGTGCT TAATTTAAAA GCCTTTGATT TTTACAAAGT GATCGAAAGT TTTATCAAAG 
     2641 CAGAAGGCAA CTTGACAAGA GAAATGATAA AACATTTAGA ACGATGTGAA CATCGAATCA 
     2701 TGGAATCCCT TGCATGGCTC TCAGATTCAC CTTTATTTGA TCTTATTAAA CAATCAAAGG 
     2761 ACCGAGAAGG ACCAACTGAT CACCTTGAAT CTGCTTGTCC TCTTAATCTT CCTCTCCAGA 
     2821 ATAATCACAC TGCAGCAGAT ATGTATCTTT CTCCTGTAAG ATCTCCAAAG AAAAAAGGTT 
     2881 CAACTACGCG TGTAAATTCT ACTGCAAATG CAGAGACACA AGCAACCTCA GCCTTCCAGA 
     2941 CCCAGAAGCC ATTGAAATCT ACCTCTCTTT CACTGTTTTA TAAAAAAGTG TATCGGCTAG 
     3001 CCTATCTCCG GCTAAATACA CTTTGTGAAC GCCTTCTGTC TGAGCACCCA GAATTAGAAC 
     3061 ATATCATCTG GACCCTTTTC CAGCACACCC TGCAGAATGA GTATGAACTC ATGAGAGACA 
     3121 GGCATTTGGA CCAAATTATG ATGTGTTCCA TGTATGGCAT ATGCAAAGTG AAGAATATAG 
     3181 ACCTTAAATT CAAAATCATT GTAACAGCAT ACAAGGATCT TCCTCATGCT GTTCAGGAGA 
     3241 CATTCAAACG TGTTTTGATC AAAGAAGAGG AGTATGATTC TATTATAGTA TTCTATAACT 
     3301 CGGTCTTCAT GCAGAGACTG AAAACAAATA TTTTGCAGTA TGCTTCCACC AGGCCCCCTA 
     3361 CCTTGTCACC AATACCTCAC ATTCCTCGAA GCCCTTACAA GTTTCCTAGT TCACCCTTAC 
     3421 GGATTCCTGG AGGGAACATC TATATTTCAC CCCTGAAGAG TCCATATAAA ATTTCAGAAG 
     3481 GTCTGCCAAC ACCAACAAAA ATGACTCCAA GATCAAGAAT CTTAGTATCA ATTGGTGAAT 
     3541 CATTCGGGAC TTCTGAGAAG TTCCAGAAAA TAAATCAGAT GGTATGTAAC AGCGACCGTG 
     3601 TGCTCAAAAG AAGTGCTGAA GGAAGCAACC CTCCTAAACC ACTGAAAAAA CTACGCTTTG 
     3661 ATATTGAAGG ATCAGATGAA GCAGATGGAA GTAAACATCT CCCAGGAGAG TCCAAATTTC 
     3721 AGCAGAAACT GGCAGAAATG ACTTCTACTC GAACACGAAT GCAAAAGCAG AAAATGAATG 
     3781 ATAGCATGGA TACCTCAAAC AAGGAAGAGA AAAGCGGACC GACGCGTACG CGGCCGCTCG 
     3841 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3901 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3961 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4021 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4081 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4141 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4201 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4261 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4321 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4381 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4441 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4501 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4561 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4621 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4681 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4741 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4801 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4861 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4921 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4981 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5041 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5101 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5161 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5221 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5281 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5341 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5401 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5461 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5521 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5581 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5641 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5701 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5761 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5821 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5881 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5941 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6001 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6061 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6121 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6181 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6241 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6301 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6361 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6421 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6481 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6541 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6601 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6661 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6721 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6781 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6841 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6901 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6961 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7021 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7081 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7141 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7201 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7261 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7321 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7381 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7441 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7501 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7561 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7621 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//