LOCUS       RC206868                6981 bp    DNA     circular  SYN 
DEFINITION  HHIP (Myc-DDK-tagged)-Human hedgehog interacting protein (HHIP)
ACCESSION   NM_022475
VERSION     NM_022475.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3138..3229 
                     /label="Myc-DDK"  
     misc_feature    3259..3844 
                     /label="PolyA signal"  
     misc_feature    3316..3335 
                     /label="Restriction Enzyme"  
     misc_feature    4120..4792 
                     /label="ColE1"  
     misc_feature    5222..6016 
                     /label="Kan/Neo"  
     misc_feature    6051..6353 
                     /label="SV40 ori"  
     misc_feature    6439..6916 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3120..3126 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3120 
                     /label="HHIP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGAAGATG CTCTCCTTTA AGCTGCTGCT GCTGGCCGTG GCTCTGGGCT 
     1081 TCTTTGAAGG AGATGCTAAG TTTGGGGAAA GAAACGAAGG GAGCGGAGCA AGGAGGAGAA 
     1141 GGTGCCTGAA TGGGAACCCC CCGAAGCGCC TGAAAAGGAG AGACAGGAGG ATGATGTCCC 
     1201 AGCTGGAGCT GCTGAGTGGG GGAGAGATGC TGTGCGGTGG CTTCTACCCT CGGCTGTCCT 
     1261 GCTGCCTGCG GAGTGACAGC CCGGGGCTAG GGCGCCTGGA GAATAAGATA TTTTCTGTTA 
     1321 CCAACAACAC AGAATGTGGG AAGTTACTGG AGGAAATCAA ATGTGCACTT TGCTCTCCAC 
     1381 ATTCTCAAAG CCTGTTCCAC TCACCTGAGA GAGAAGTCTT GGAAAGAGAC CTAGTACTTC 
     1441 CTCTGCTCTG CAAAGACTAT TGCAAAGAAT TCTTTTACAC TTGCCGAGGC CATATTCCAG 
     1501 GTTTCCTTCA AACAACTGCG GATGAGTTTT GCTTTTACTA TGCAAGAAAA GATGGTGGGT 
     1561 TGTGCTTTCC AGATTTTCCA AGAAAACAAG TCAGAGGACC AGCATCTAAC TACTTGGACC 
     1621 AGATGGAAGA ATATGACAAA GTGGAAGAGA TCAGCAGAAA GCACAAACAC AACTGCTTCT 
     1681 GTATTCAGGA GGTTGTGAGT GGGCTGCGGC AGCCCGTTGG TGCCCTGCAT AGTGGGGATG 
     1741 GCTCGCAACG TCTCTTCATT CTGGAAAAAG AAGGTTATGT GAAGATACTT ACCCCTGAAG 
     1801 GAGAAATTTT CAAGGAGCCT TATTTGGACA TTCACAAACT TGTTCAAAGT GGAATAAAGG 
     1861 GAGGAGATGA AAGAGGACTG CTAAGCCTCG CATTCCATCC CAATTACAAG AAAAATGGAA 
     1921 AGTTGTATGT GTCCTATACC ACCAACCAAG AACGGTGGGC TATCGGGCCT CATGACCACA 
     1981 TTCTTAGGGT TGTGGAATAC ACAGTATCCA GAAAAAATCC ACACCAAGTT GATTTGAGAA 
     2041 CAGCCAGAGT CTTTCTTGAA GTTGCAGAAC TCCACAGAAA GCATCTGGGA GGACAACTGC 
     2101 TCTTTGGCCC TGACGGCTTT TTGTACATCA TTCTTGGTGA TGGGATGATT ACACTGGATG 
     2161 ATATGGAAGA AATGGATGGG TTAAGTGATT TCACAGGCTC AGTGCTACGG CTGGATGTGG 
     2221 ACACAGACAT GTGCAACGTG CCTTATTCCA TACCAAGGAG CAACCCACAC TTCAACAGCA 
     2281 CCAACCAGCC CCCCGAAGTG TTTGCTCATG GGCTCCACGA TCCAGGCAGA TGTGCTGTGG 
     2341 ATAGACATCC CACTGATATA AACATCAATT TAACGATACT GTGTTCAGAC TCCAATGGAA 
     2401 AAAACAGATC ATCAGCCAGA ATTCTACAGA TAATAAAGGG GAAAGATTAT GAAAGTGAGC 
     2461 CATCACTTTT AGAATTCAAG CCATTCAGTA ATGGTCCTTT GGTTGGTGGA TTTGTATACC 
     2521 GGGGCTGCCA GTCAGAAAGA TTGTATGGAA GCTACGTGTT TGGAGATCGT AATGGGAATT 
     2581 TCCTAACTCT CCAGCAAAGT CCTGTGACAA AGCAGTGGCA AGAAAAACCA CTCTGTCTCG 
     2641 GCACTAGTGG GTCCTGTAGA GGCTACTTTT CCGGTCACAT CTTGGGATTT GGAGAAGATG 
     2701 AACTAGGTGA AGTTTACATT TTATCAAGCA GTAAAAGTAT GACCCAGACT CACAATGGAA 
     2761 AACTCTACAA AATTGTAGAT CCCAAAAGAC CTTTAATGCC TGAGGAATGC AGAGCCACGG 
     2821 TACAACCTGC ACAGACACTG ACTTCAGAGT GCTCCAGGCT CTGTCGAAAC GGCTACTGCA 
     2881 CCCCCACGGG AAAGTGCTGC TGCAGTCCAG GCTGGGAGGG GGACTTCTGC AGAACTGCAA 
     2941 AATGTGAGCC AGCATGTCGT CATGGAGGTG TCTGTGTTAG ACCGAACAAG TGCCTCTGTA 
     3001 AAAAAGGATA TCTTGGTCCT CAATGTGAAC AAGTGGACAG AAACATCCGC AGAGTGACCA 
     3061 GGGCAGGTAT TCTTGATCAG ATCATTGACA TGACATCTTA CTTGCTGGAT CTAACAAGTT 
     3121 ACATTGTAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3181 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3241 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3301 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3361 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3421 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3481 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3541 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3601 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     3661 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     3721 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     3781 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     3841 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     3901 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     3961 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4021 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4081 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4141 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4201 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4261 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4321 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4381 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4441 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4501 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4561 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4621 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     4681 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     4741 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     4801 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     4861 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     4921 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     4981 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5041 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5101 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5161 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5221 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5281 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5341 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5401 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5461 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5521 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     5581 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     5641 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     5701 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     5761 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     5821 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     5881 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     5941 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6001 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6061 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6121 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6181 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6241 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6301 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6361 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6421 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6481 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6541 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     6601 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     6661 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     6721 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     6781 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     6841 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     6901 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     6961 CAAAAATTTA ACGCGAATTT T
//