LOCUS       RC206863                8688 bp    DNA     circular  SYN 
DEFINITION  FLII (Myc-DDK-tagged)-Human flightless I homolog (Drosophila) (FLII)
ACCESSION   NM_002018
VERSION     NM_002018.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4845..4936 
                     /label="Myc-DDK"  
     misc_feature    4966..5551 
                     /label="PolyA signal"  
     misc_feature    5023..5042 
                     /label="Restriction Enzyme"  
     misc_feature    5827..6499 
                     /label="ColE1"  
     misc_feature    6929..7723 
                     /label="Kan/Neo"  
     misc_feature    7758..8060 
                     /label="SV40 ori"  
     misc_feature    8146..8623 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4827..4833 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4827 
                     /label="FLII"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCCACC GGGGTGCTGC CGTTCGTGCG TGGCGTGGAC CTCAGCGGCA 
     1081 ACGACTTCAA GGGCGGCTAC TTCCCTGAGA ATGTCAAGGC CATGACCAGC CTGCGGTGGC 
     1141 TGAAGCTGAA CCGCACTGGC CTCTGCTACC TGCCCGAGGA GCTGGCCGCC CTGCAGAAGC 
     1201 TGGAACACTT GTCTGTGAGC CACAACAACC TGACCACGCT TCATGGGGAG CTGTCCAGCC 
     1261 TGCCATCGCT GCGCGCCATC GTGGCCCGAG CCAACAGTCT GAAGAATTCC GGAGTCCCCG 
     1321 ATGACATCTT CAAGCTAGAT GATCTCTCAG TCCTGGACTT GAGCCACAAC CAGCTGACAG 
     1381 AGTGCCCGCG GGAGCTGGAG AACGCCAAGA ACATGCTGGT GCTGAACCTC AGCCACAACA 
     1441 GCATCGACAC CATCCCCAAC CAGCTCTTCA TCAACCTCAC TGACCTACTA TACCTGGACC 
     1501 TCAGCGAGAA CCGCCTGGAG AGCCTGCCCC CGCAGATGCG CCGCCTGGTG CACCTGCAGA 
     1561 CGCTCGTGCT CAATGGAAAC CCCCTGCTGC ATGCACAGCT CCGGCAGCTC CCAGCGATGA 
     1621 CGGCCCTGCA GACCCTGCAC CTGCGGAGCA CCCAGCGCAC CCAGAGCAAC CTGCCCACCA 
     1681 GCCTGGAGGG TCTGAGCAAC CTCGCAGACG TGGATCTGTC CTGCAATGAC CTGACACGGG 
     1741 TGCCCGAGTG TCTGTACACC CTCCCCAGCC TGCGCCGCCT CAACCTCAGC AGCAACCAGA 
     1801 TCACGGAGCT GTCCCTGTGC ATAGACCAGT GGGTGCACGT GGAAACTCTG AACCTGTCCC 
     1861 GAAATCAGCT CACCTCACTG CCCTCAGCCA TTTGCAAGCT GAGCAAGCTG AAGAAGCTGT 
     1921 ACCTGAATTC CAACAAGCTG GACTTTGACG GGCTGCCCTC AGGCATTGGC AAGCTCACCA 
     1981 ACCTGGAAGA GTTCATGGCT GCCAACAACA ACCTGGAGCT GGTCCCTGAA AGTCTCTGCA 
     2041 GGTGCCCAAA GCTGAGGAAA CTTGTCCTGA ACAAGAACCA CCTGGTGACC CTCCCAGAAG 
     2101 CCATCCATTT CCTGACGGAG ATCGAGGTCC TGGATGTGCG GGAGAACCCC AACCTGGTCA 
     2161 TGCCGCCCAA GCCCGCAGAC CGTGCCGCTG AGTGGTACAA CATCGACTTC TCGCTGCAGA 
     2221 ACCAGCTGCG GCTAGCGGGT GCCTCTCCTG CTACCGTGGC TGCAGCTGCA GCTGCAGGGA 
     2281 GTGGGCCCAA GGACCCTATG GCTCGCAAGA TGCGACTGCG GAGGCGCAAG GATTCAGCCC 
     2341 AGGATGACCA GGCCAAGCAG GTGCTGAAGG GCATGTCAGA TGTTGCCCAG GAGAAGAACA 
     2401 AAAAGCAGGA GGAGAGCGCA GATGCCCGGG CCCCCAGCGG GAAGGTGCGG CGTTGGGACC 
     2461 AGGGCCTGGA GAAGCCCCGC CTTGACTACT CCGAGTTCTT CACGGAGGAC GTGGGCCAGC 
     2521 TGCCCGGACT GACCATCTGG CAGATAGAGA ACTTCGTGCC TGTGCTGGTG GAGGAAGCCT 
     2581 TCCACGGCAA GTTCTACGAG GCTGACTGCT ACATTGTGCT CAAGACCTTT CTGGATGACA 
     2641 GCGGCTCCCT CAACTGGGAG ATCTACTACT GGATTGGCGG GGAGGCCACA CTCGACAAGA 
     2701 AAGCTTGCTC TGCCATCCAC GCTGTCAACT TGCGCAACTA CCTGGGTGCT GAGTGCCGCA 
     2761 CTGTCCGGGA GGAGATGGGC GATGAGAGCG AGGAGTTCCT GCAGGTGTTT GACAACGACA 
     2821 TCTCCTACAT TGAGGGTGGA ACAGCCAGTG GCTTCTACAC TGTGGAAGAC ACACACTATG 
     2881 TCACCAGGAT GTATCGTGTG TATGGGAAAA AGAACATCAA GTTGGAGCCT GTGCCCCTCA 
     2941 AGGGGACCTC TCTGGACCCA AGGTTTGTTT TCCTGCTGGA CCGAGGGCTA GACATCTACG 
     3001 TATGGCGGGG GGCCCAGGCC ACACTGAGCA GCACCACCAA GGCCAGGCTC TTTGCAGAGA 
     3061 AAATTAACAA GAATGAGCGG AAAGGGAAGG CTGAGATCAC ACTGCTGGTG CAGGGCCAGG 
     3121 AGCTCCCAGA GTTCTGGGAG GCACTGGGTG GGGAGCCCTC TGAGATCAAG AAGCACGTGC 
     3181 CTGAAGACTT CTGGCCGCCG CAGCCCAAGC TGTACAAGGT GGGCCTGGGC TTGGGCTACC 
     3241 TGGAGCTGCC ACAGATCAAC TACAAGCTCT CCGTGGAACA TAAGCAGCGT CCCAAGGTGG 
     3301 AGCTGATGCC AAGAATGCGG CTGCTGCAGA GTCTGCTGGA CACGCGCTGC GTGTACATTC 
     3361 TGGACTGTTG GTCCGACGTG TTCATCTGGC TCGGCCGCAA GTCCCCGCGC CTGGTGCGCG 
     3421 CTGCCGCCCT CAAGCTGGGT CAGGAGCTGT GCGGGATGCT GCACCGGCCA CGCCATGCCA 
     3481 CGGTCAGCCG CAGCCTCGAG GGCACCGAGG CGCAGGTGTT CAAGGCCAAG TTCAAGAATT 
     3541 GGGACGATGT GTTGACGGTG GACTACACAC GCAATGCGGA GGCCGTGCTG CAGAGCCCGG 
     3601 GTCTCTCCGG GAAGGTGAAA CGCGACGCCG AGAAGAAAGA CCAGATGAAG GCTGACCTCA 
     3661 CTGCGCTTTT CCTGCCGCGG CAGCCGCCCA TGTCGCTGGC CGAGGCGGAG CAGCTGATGG 
     3721 AGGAGTGGAA CGAAGACCTA GACGGCATGG AGGGTTTCGT GCTGGAGGGC AAGAAGTTTG 
     3781 CGCGGCTGCC GGAAGAGGAG TTTGGCCACT TCTACACGCA GGACTGCTAC GTCTTCCTCT 
     3841 GCAGGTACTG GGTGCCTGTG GAGTACGAGG AGGAGGAAAA GAAGGAAGAC AAGGAGGAGA 
     3901 AGGCCGAGGG CAAAGAAGGC GAGGAAGCAA CCGCTGAGGC AGAGGAGAAG CAGCCAGAGG 
     3961 AGGACTTCCA GTGCATCGTG TACTTCTGGC AGGGCCGTGA AGCCTCCAAT ATGGGCTGGC 
     4021 TCACCTTCAC CTTCAGCCTG CAAAAGAAGT TCGAGAGCCT CTTCCCTGGG AAGCTGGAGG 
     4081 TGGTACGCAT GACGCAGCAG CAGGAGAACC CCAAGTTCCT GTCCCATTTC AAGAGGAAGT 
     4141 TCATCATCCA CCGGGGCAAG AGGAAGGCGG TCCAGGGCGC CCAACAGCCC AGCCTCTACC 
     4201 AGATCCGCAC CAACGGCAGC GCCCTCTGCA CCCGGTGCAT CCAGATCAAC ACCGACTCCA 
     4261 GCCTCCTCAA CTCCGAGTTC TGCTTCATCC TCAAGGTTCC CTTTGAGAGT GAGGACAACC 
     4321 AGGGCATCGT GTATGCCTGG GTGGGCCGGG CATCAGACCC TGACGAAGCC AAGTTGGCAG 
     4381 AAGACATCCT GAACACCATG TTTGACACCT CCTACAGCAA GCAGGTTATC AACGAAGGTG 
     4441 AGGAGCCTGA GAACTTCTTC TGGGTGGGCA TTGGGGCACA GAAGCCCTAT GATGACGATG 
     4501 CCGAGTACAT GAAACACACA CGTCTCTTCC GGTGCTCCAA CGAGAAGGGC TACTTTGCAG 
     4561 TGACTGAGAA ATGCTCCGAC TTTTGCCAAG ATGACCTGGC AGATGATGAC ATCATGTTGC 
     4621 TAGACAATGG CCAAGAGGTC TACATGTGGG TGGGGACCCA GACTAGCCAG GTGGAGATCA 
     4681 AGCTGAGCCT GAAGGCCTGC CAGGTATATA TCCAGCACAT GCGGTCCAAG GAACATGAGC 
     4741 GGCCGCGCCG GCTGCGCCTG GTCCGCAAGG GCAATGAGCA GCACGCCTTT ACCCGCTGCT 
     4801 TCCACGCCTG GAGCGCCTTC TGCAAGGCCC TGGCCACGCG TACGCGGCCG CTCGAGCAGA 
     4861 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4921 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4981 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5041 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5101 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5161 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5221 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5281 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5341 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5401 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5461 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5521 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5581 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5641 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5701 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5761 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5821 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5881 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5941 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6001 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6061 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6121 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6181 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6241 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6301 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6361 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6421 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6481 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6541 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6601 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6661 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6721 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6781 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6841 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6901 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6961 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7021 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7081 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7141 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7201 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7261 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7321 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7381 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7441 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7501 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7561 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7621 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7681 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7741 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7801 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7861 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7921 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7981 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8041 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8101 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8161 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8221 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8281 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8341 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8401 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8461 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8521 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8581 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8641 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//