LOCUS       RC206661                6972 bp    DNA     circular  SYN 
DEFINITION  WDR49 (Myc-DDK-tagged)-Human WD repeat domain 49 (WDR49)
ACCESSION   NM_178824
VERSION     NM_178824.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3129..3220 
                     /label="Myc-DDK"  
     misc_feature    3250..3835 
                     /label="PolyA signal"  
     misc_feature    3307..3326 
                     /label="Restriction Enzyme"  
     misc_feature    4111..4783 
                     /label="ColE1"  
     misc_feature    5213..6007 
                     /label="Kan/Neo"  
     misc_feature    6042..6344 
                     /label="SV40 ori"  
     misc_feature    6430..6907 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3111..3117 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3111 
                     /label="WDR49"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTGGAGA GAGAAATCAA AAAAGCGTCT TAATATGACA TCCTTCAACA 
     1081 TTGCCCAGGG CATTCATGCT TTTGATTATC ACTCTCGGCT CAATTTAATT GCAACTGCTG 
     1141 GCATTAACAA TAAAGTTTGC CTTTGGAATC CCTATGTTGT CTCTAAACCA GTGGGTGTCC 
     1201 TTTGGGGCCA CTCAGCCAGT GTAATAGCCG TCCAATTCTT TGTGGAAAGA AAACAACTTT 
     1261 TCAGCTTCTC CAAGGATAAA GTTTTGAGAC TCTGGGATAT TCAACACCAG CTGTCCATCC 
     1321 AGAGGATAGC TTGTTCTTTC CCCAAAAGTC AGGACTTCAG ATGTCTCTTC CACTTTGATG 
     1381 AAGCCCATGG ACGACTTTTC ATCTCGTTTA ATAACCAGCT AGCATTGTTG GCAATGAAAA 
     1441 GTGAAGCCAG CAAGAGGGTG AAAAGCCATG AGAAAGCAGT CACTTGTGTT CTTTACAATT 
     1501 CTATCTTGAA GCAGGTAATC AGCTCTGATA CAGGGTCTAC TGTTTCCTTC TGGATGATAG 
     1561 ACACTGGGCA GAAAATCAAA CAGTTTACTG GTTGCCACGG CAACGCAGAA ATCAGCACTA 
     1621 TGGCCCTTGA TGCAAATGAG ACTCGGCTTT TGACTGGCAG CACAGATGGG ACTGTAAAGA 
     1681 TATGGGACTT CAATGGATAT TGTCACCATA CACTAAATGT TGGGCAAGAT GGAGCTGTGG 
     1741 ATATTTCACA AATCCTCATT CTTAAGAAGA AAATACTGGT TACAGGCTGG GAGAGGGCTA 
     1801 TTACTGTGTT TCGACCCCAA AACTTCAATC AATTTTTCAT CCAGCCTGAA GAATGGAAAG 
     1861 GAGGTATACA GCACCATGAT GACATCTTGT GTGCTGCGTT TTTACCTCCA CAAACTCTTG 
     1921 TTACGGGGAG TTATGATGGA GAAATTGTTC TATGGAACAA TAGCACAGAG AATGCTCACC 
     1981 ATGTTCTTCA CCCTGATTAC CAGAGGTTGC TAAAGTCAAA ATTAGATACA AAACCTCAAA 
     2041 AACTTCTCAG TGCTGGGAGA AGCCAACCCT CCCACCCCAT GGCAGACCAT TCTACCACGG 
     2101 GAGTCCGCAA CTTTGAGATT GACACTGAGG GCAAAAATGC TGTTATGAGA CTTTGCTTTC 
     2161 TTAAAGCAAG AAAAAACACT GCAGTGACAG GAGGAGCTAA CCTGGTATCA TGTGGAGGAT 
     2221 CTGGTTATGT CAGATTTTGG GATATATATA AGAAGCAACT TCTGGCTGAA TTTTTGGCTC 
     2281 ATAGTGGAGT TGGATCGATT ATTATGTCTA CTGATAAGAT GAATCGATAC CTTACCACAG 
     2341 GAGATCTTGA TGGATGGTTG AAAATCTGGA ATATAGAGGA GTACTGTCTT AACTCCAGTA 
     2401 AGAACAAAAT CACCAAGGCC CCAACTCTGA TAAGATCATT CCAACCTCAT GAGGACCGAA 
     2461 TAAGTTCCTT AGAGATGTGT GAGCCAGGTG GTCAGTTACT GATTATCTCC TCCTCTGCAG 
     2521 ACTGCAGTAT TTGTGTGACT GGTGTCTGCA ATGCTCCTGT TTGGATCTTT GGTCAGGCAA 
     2581 AGCACTGGCA TATTGAAAAC TGCCTTTTCC TTCCTAAAAG AGATACTAAT TTAGTGGAAA 
     2641 GTGAGATTCA AAAGGAAATT TCTTTATTTT CTAAGGAGGA ATCTTGTTTA GACCCAACAG 
     2701 AACATTCTCT ACTTAATAAG AAAAACAAAG ATGACTCAAC ATACAATGTC AGACCATCAG 
     2761 AAGATATAAA TTTAGATATA AAATATAAGG AAAGAAGTAC CTGCATGAAA GAAACACAAA 
     2821 AACCTTATTA TGGTGAAGTT ATAAAAAAAT CATTCAGTAC ATTTAGGTCA TTAAACATTG 
     2881 GAGCCCTGGA AGAGCTGCCT GAAGTGAATA AACCTGCTTT CCTTCTAGAC CCTGAGAAAT 
     2941 ACTTTAGGAA AGAACCAGAG GAAGAGCGTC CCCAAATTCT GGAGGCCCCG TCACTTTTTA 
     3001 AAACTTTGAA AGCTGTATTT GATGAGAAAA ACCTGTTTCC CAAGGAAATT CTGCATCATG 
     3061 AACGAAAAGC CAAGCAATTA TGCCAAGAAA AAAGTTGTGA AGTGAAGAAA AATAAGAAGA 
     3121 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3181 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3241 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3301 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3361 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3421 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3481 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3541 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     3601 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     3661 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     3721 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     3781 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     3841 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     3901 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     3961 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4021 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4081 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4141 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4201 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4261 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4321 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4381 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4441 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4501 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4561 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     4621 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     4681 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     4741 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     4801 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     4861 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     4921 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     4981 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5041 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5101 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5161 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5221 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5281 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5341 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5401 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5461 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5521 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     5581 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     5641 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     5701 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     5761 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     5821 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     5881 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     5941 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6001 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6061 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6121 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6181 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6241 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6301 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6361 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6421 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6481 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6541 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     6601 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     6661 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     6721 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     6781 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     6841 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     6901 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     6961 AACGCGAATT TT
//