LOCUS       RC206554                8253 bp    DNA     circular  SYN 
DEFINITION  TEK (Myc-DDK-tagged)-Human TEK tyrosine kinase, endothelial (TEK)
ACCESSION   NM_000459
VERSION     NM_000459.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4410..4501 
                     /label="Myc-DDK"  
     misc_feature    4531..5116 
                     /label="PolyA signal"  
     misc_feature    4588..4607 
                     /label="Restriction Enzyme"  
     misc_feature    5392..6064 
                     /label="ColE1"  
     misc_feature    6494..7288 
                     /label="Kan/Neo"  
     misc_feature    7323..7625 
                     /label="SV40 ori"  
     misc_feature    7711..8188 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4392..4398 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4392 
                     /label="TEK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTCTTTA GCCAGCTTAG TTCTCTGTGG AGTCAGCTTG CTCCTTTCTG 
     1081 GAACTGTGGA AGGTGCCATG GACTTGATCT TGATCAATTC CCTACCTCTT GTATCTGATG 
     1141 CTGAAACATC TCTCACCTGC ATTGCCTCTG GGTGGCGCCC CCATGAGCCC ATCACCATAG 
     1201 GAAGGGACTT TGAAGCCTTA ATGAACCAGC ACCAGGATCC GCTGGAAGTT ACTCAAGATG 
     1261 TGACCAGAGA ATGGGCTAAA AAAGTTGTTT GGAAGAGAGA AAAGGCTAGT AAGATCAATG 
     1321 GTGCTTATTT CTGTGAAGGG CGAGTTCGAG GAGAGGCAAT CAGGATACGA ACCATGAAGA 
     1381 TGCGTCAACA AGCTTCCTTC CTACCAGCTA CTTTAACTAT GACTGTGGAC AAGGGAGATA 
     1441 ACGTGAACAT ATCTTTCAAA AAGGTATTGA TTAAAGAAGA AGATGCAGTG ATTTACAAAA 
     1501 ATGGTTCCTT CATCCATTCA GTGCCCCGGC ATGAAGTACC TGATATTCTA GAAGTACACC 
     1561 TGCCTCATGC TCAGCCCCAG GATGCTGGAG TGTACTCGGC CAGGTATATA GGAGGAAACC 
     1621 TCTTCACCTC GGCCTTCACC AGGCTGATAG TCCGGAGATG TGAAGCCCAG AAGTGGGGAC 
     1681 CTGAATGCAA CCATCTCTGT ACTGCTTGTA TGAACAATGG TGTCTGCCAT GAAGATACTG 
     1741 GAGAATGCAT TTGCCCTCCT GGGTTTATGG GAAGGACGTG TGAGAAGGCT TGTGAACTGC 
     1801 ACACGTTTGG CAGAACTTGT AAAGAAAGGT GCAGTGGACA AGAGGGATGC AAGTCTTATG 
     1861 TGTTCTGTCT CCCTGACCCC TATGGGTGTT CCTGTGCCAC AGGCTGGAAG GGTCTGCAGT 
     1921 GCAATGAAGC ATGCCACCCT GGTTTTTACG GGCCAGATTG TAAGCTTAGG TGCAGCTGCA 
     1981 ACAATGGGGA GATGTGTGAT CGCTTCCAAG GATGTCTCTG CTCTCCAGGA TGGCAGGGGC 
     2041 TCCAGTGTGA GAGAGAAGGC ATACCGAGGA TGACCCCAAA GATAGTGGAT TTGCCAGATC 
     2101 ATATAGAAGT AAACAGTGGT AAATTTAATC CCATTTGCAA AGCTTCTGGC TGGCCGCTAC 
     2161 CTACTAATGA AGAAATGACC CTGGTGAAGC CGGATGGGAC AGTGCTCCAT CCAAAAGACT 
     2221 TTAACCATAC GGATCATTTC TCAGTAGCCA TATTCACCAT CCACCGGATC CTCCCCCCTG 
     2281 ACTCAGGAGT TTGGGTCTGC AGTGTGAACA CAGTGGCTGG GATGGTGGAA AAGCCCTTCA 
     2341 ACATTTCTGT TAAAGTTCTT CCAAAGCCCC TGAATGCCCC AAACGTGATT GACACTGGAC 
     2401 ATAACTTTGC TGTCATCAAC ATCAGCTCTG AGCCTTACTT TGGGGATGGA CCAATCAAAT 
     2461 CCAAGAAGCT TCTATACAAA CCCGTTAATC ACTATGAGGC TTGGCAACAT ATTCAAGTGA 
     2521 CAAATGAGAT TGTTACACTC AACTATTTGG AACCTCGGAC AGAATATGAA CTCTGTGTGC 
     2581 AACTGGTCCG TCGTGGAGAG GGTGGGGAAG GGCATCCTGG ACCTGTGAGA CGCTTCACAA 
     2641 CAGCTTCTAT CGGACTCCCT CCTCCAAGAG GTCTAAATCT CCTGCCTAAA AGTCAGACCA 
     2701 CTCTAAATTT GACCTGGCAA CCAATATTTC CAAGCTCGGA AGATGACTTT TATGTTGAAG 
     2761 TGGAGAGAAG GTCTGTGCAA AAAAGTGATC AGCAGAATAT TAAAGTTCCA GGCAACTTGA 
     2821 CTTCGGTGCT ACTTAACAAC TTACATCCCA GGGAGCAGTA CGTGGTCCGA GCTAGAGTCA 
     2881 ACACCAAGGC CCAGGGGGAA TGGAGTGAAG ATCTCACTGC TTGGACCCTT AGTGACATTC 
     2941 TTCCTCCTCA ACCAGAAAAC ATCAAGATTT CCAACATTAC ACACTCCTCG GCTGTGATTT 
     3001 CTTGGACAAT ATTGGATGGC TATTCTATTT CTTCTATTAC TATCCGTTAC AAGGTTCAAG 
     3061 GCAAGAATGA AGACCAGCAC GTTGATGTGA AGATAAAGAA TGCCACCATC ACTCAGTATC 
     3121 AGCTCAAGGG CCTAGAGCCT GAAACAGCAT ACCAGGTGGA CATTTTTGCA GAGAACAACA 
     3181 TAGGGTCAAG CAACCCAGCC TTTTCTCATG AACTGGTGAC CCTCCCAGAA TCTCAAGCAC 
     3241 CAGCGGACCT CGGAGGGGGG AAGATGCTGC TTATAGCCAT CCTTGGCTCT GCTGGAATGA 
     3301 CCTGCCTGAC TGTGCTGTTG GCCTTTCTGA TCATATTGCA ATTGAAGAGA GCAAATGTGC 
     3361 AAAGGAGAAT GGCCCAAGCC TTCCAAAACG TGAGGGAAGA ACCAGCTGTG CAGTTCAACT 
     3421 CAGGGACTCT GGCCCTAAAC AGGAAGGTCA AAAACAACCC AGATCCTACA ATTTATCCAG 
     3481 TGCTTGACTG GAATGACATC AAATTTCAAG ATGTGATTGG GGAGGGCAAT TTTGGCCAAG 
     3541 TTCTTAAGGC GCGCATCAAG AAGGATGGGT TACGGATGGA TGCTGCCATC AAAAGAATGA 
     3601 AAGAATATGC CTCCAAAGAT GATCACAGGG ACTTTGCAGG AGAACTGGAA GTTCTTTGTA 
     3661 AACTTGGACA CCATCCAAAC ATCATCAATC TCTTAGGAGC ATGTGAACAT CGAGGCTACT 
     3721 TGTACCTGGC CATTGAGTAC GCGCCCCATG GAAACCTTCT GGACTTCCTT CGCAAGAGCC 
     3781 GTGTGCTGGA GACGGACCCA GCATTTGCCA TTGCCAATAG CACCGCGTCC ACACTGTCCT 
     3841 CCCAGCAGCT CCTTCACTTC GCTGCCGACG TGGCCCGGGG CATGGACTAC TTGAGCCAAA 
     3901 AACAGTTTAT CCACAGGGAT CTGGCTGCCA GAAACATTTT AGTTGGTGAA AACTATGTGG 
     3961 CAAAAATAGC AGATTTTGGA TTGTCCCGAG GTCAAGAGGT GTATGTGAAA AAGACAATGG 
     4021 GAAGGCTCCC AGTGCGCTGG ATGGCCATCG AGTCACTGAA TTACAGTGTG TACACAACCA 
     4081 ACAGTGATGT ATGGTCCTAT GGTGTGTTAC TATGGGAGAT TGTTAGCTTA GGAGGCACAC 
     4141 CCTACTGCGG GATGACTTGT GCAGAACTCT ACGAGAAGCT GCCCCAGGGC TACAGACTGG 
     4201 AGAAGCCCCT GAACTGTGAT GATGAGGTGT ATGATCTAAT GAGACAATGC TGGCGGGAGA 
     4261 AGCCTTATGA GAGGCCATCA TTTGCCCAGA TATTGGTGTC CTTAAACAGA ATGTTAGAGG 
     4321 AGCGAAAGAC CTACGTGAAT ACCACGCTTT ATGAGAAGTT TACTTATGCA GGAATTGACT 
     4381 GTTCTGCTGA AGAAGCGGCC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4441 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4501 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4561 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4621 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4681 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4741 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4801 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4861 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4921 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4981 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5041 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5101 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5161 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5221 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5281 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5341 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5401 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5461 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5521 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5581 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5641 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5701 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5761 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5821 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5881 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5941 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6001 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6061 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6121 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6181 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6241 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6301 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6361 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6421 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6481 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6541 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6601 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6661 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6721 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6781 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6841 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6901 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6961 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7021 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7081 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7141 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7201 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7261 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7321 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7381 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7441 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7501 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7561 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7621 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7681 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7741 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7801 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7861 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7921 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7981 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8041 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8101 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8161 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8221 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//