LOCUS       RC206498                7131 bp    DNA     circular  SYN 
DEFINITION  POMT2 (Myc-DDK-tagged)-Human protein-O-mannosyltransferase 2 (POMT2)
ACCESSION   NM_013382
VERSION     NM_013382.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3288..3379 
                     /label="Myc-DDK"  
     misc_feature    3409..3994 
                     /label="PolyA signal"  
     misc_feature    3466..3485 
                     /label="Restriction Enzyme"  
     misc_feature    4270..4942 
                     /label="ColE1"  
     misc_feature    5372..6166 
                     /label="Kan/Neo"  
     misc_feature    6201..6503 
                     /label="SV40 ori"  
     misc_feature    6589..7066 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3270..3276 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3270 
                     /label="POMT2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGCCGGCC ACGGGCGGAG GCCTGGCAGA GTCCGAGCTG CGTCCCCGGA 
     1081 GGGGCCGCTG TGGCCCCCAG GCTGCTAGGG CCGCAGGCCG GGACGTGGCC GCTGAGGCTG 
     1141 TGGCGCGAAG CCCCAAACGG CCTGCTTGGG GCTCACGGCG CTTCGAGGCT GTCGGCTGGT 
     1201 GGGCCCTGCT GGCCTTGGTG ACGCTGCTGT CCTTCGCCAC CCGCTTCCAC CGCTTGGACG 
     1261 AGCCGCCGCA CATCTGTTGG GATGAGACTC ACTTTGGAAA AATGGGAAGT TACTATATCA 
     1321 ACCGTACATT TTTCTTTGAT GTGCACCCGC CCCTGGGAAA GATGCTGATA GGTCTTGCTG 
     1381 GCTACCTGAG TGGATATGAT GGTACCTTTT TGTTCCAGAA GCCTGGGGAT AAATATGAGC 
     1441 ATCACAGCTA CATGGGAATG AGAGGATTCT GTGCATTCCT TGGCTCCTGG CTGGTCCCCT 
     1501 TTGCCTACCT CACTGTACTG GATCTGTCCA AGTCCCTCTC GGCAGCACTG CTCACAGCTG 
     1561 CCCTCCTCAC CTTTGACACG GGATGCCTCA CTCTGTCCCA GTACATCCTC CTTGACCCCA 
     1621 TCCTGATGTT CTTCATCATG GCTGCCATGC TGAGCATGGT CAAGTACAAC TCTTGCGCCG 
     1681 ACAGGCCCTT CTCTGCCCCC TGGTGGTTCT GGCTCAGCCT GACTGGCGTT AGTCTTGCTG 
     1741 GTGCTTTAGG GGTCAAGTTT GTTGGCCTCT TTATCATCCT TCAAGTGGGG CTGAACACCA 
     1801 TTGCAGACCT TTGGTACCTG TTCGGAGACC TCAGTCTTTC ATTGGTGACT GTGGGAAAAC 
     1861 ACCTGACTGC TCGTGTCCTG TGCCTCATAG TGCTGCCCCT GGCTCTCTAT ACAGCCACCT 
     1921 TTGCTGTTCA CTTCATGGTG CTGAGTAAAA GTGGCCCTGG TGACGGTTTC TTCAGTTCTG 
     1981 CCTTCCAGGC CCGGCTTTCA GGGAACAACC TGCACAATGC TTCCATCCCT GAACACCTGG 
     2041 CCTACGGCTC TGTGATCACT GTGAAGAACC TCCGGATGGC CATCGGCTAT CTGCACTCCC 
     2101 ACAGGCACCT CTACCCCGAG GGCATTGGTG CCCGTCAGCA GCAGGTCACC ACCTATTTGC 
     2161 ACAAGGACTA CAACAACCTG TGGATTATCA AGAAACATAA CACAAACTCA GATCCCCTAG 
     2221 ACCCTTCCTT CCCAGTGGAG TTTGTAAGAC ATGGAGACAT TATTCGACTA GAACACAAAG 
     2281 AAACTTCCCG GAACTTGCAC AGTCACTATC ATGAGGCCCC CATGACCCGG AAGCACTATC 
     2341 AGGTCACCGG CTATGGCATA AATGGAACAG GGGACTCAAA TGATTTCTGG CGGATTGAGG 
     2401 TCGTAAACAG AAAATTTGGA AACCGGATCA AAGTGCTGAG AAGTCGAATT CGCTTCATCC 
     2461 ATTTGGTCAC AGGTTGTGTC CTGGGCTCCT CGGGAAAGGT TCTGCCCAAG TGGGGCTGGG 
     2521 AGCAGTTGGA AGTTACTTGC ACCCCATACC TGAAAGAAAC CCTCAACTCC ATCTGGAATG 
     2581 TGGAGGACCA TATCAATCCC AAGTTGCCAA ACATCAGCCT GGATGTGCTA CAGCCCAGTT 
     2641 TTCCTGAGAT CTTGCTGGAA TCCCACATGG TCATGATCCG GGGGAACAGT GGCCTCAAAC 
     2701 CCAAGGACAA TGAGTTCACG TCCAAACCCT GGCACTGGCC TATCAACTAT CAGGGCCTAC 
     2761 GCTTCTCAGG GGTCAATGAC ACAGATTTCC GAGTCTATCT GCTTGGCAAC CCGGTGGTTT 
     2821 GGTGGCTGAA TCTGTTGAGC ATCGCCCTCT ACCTCCTCTC AGGGAGCATC ATTGCTGTAG 
     2881 CCATGCAGAG AGGGGCACGG CTGCCAGCGG AGGTTGCAGG GTTGTCCCAG GTCCTGCTGC 
     2941 GAGGAGGCGG CCAGGTCCTG CTCGGCTGGA CACTCCATTA CTTCCCGTTT TTCCTGATGG 
     3001 GCCGGGTCCT CTACTTCCAC CACTACTTCC CAGCCATGCT CTTCTCAAGC ATGTTGACAG 
     3061 GCATTCTGTG GGACACCCTC CTGCGGCTCT GTGCCTGGGG CTTGGCCTCA TGGCCCCTGG 
     3121 CGAGGGGCAT ACATGTGGCG GGAATCCTGA GCCTGCTCCT GGGAACTGCC TACAGCTTCT 
     3181 ACCTCTTCCA CCCTCTGGCT TACGGGATGG TTGGTCCCCT GGCCCAGGAC CCCCAAAGTC 
     3241 CAATGGCAGG ACTAAGGTGG CTGGACTCAT GGGACTTTAC GCGTACGCGG CCGCTCGAGC 
     3301 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3361 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3421 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3481 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3541 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3601 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3661 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3721 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3781 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     3841 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     3901 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     3961 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4021 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4081 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4141 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4201 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4261 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4321 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4381 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4441 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4501 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4561 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4621 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4681 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4741 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4801 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     4861 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     4921 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     4981 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5041 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5101 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5161 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5221 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5281 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5341 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5401 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5461 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5521 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5581 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5641 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5701 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5761 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     5821 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     5881 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     5941 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6001 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6061 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6121 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6181 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6241 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6301 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6361 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6421 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6481 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6541 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6601 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6661 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6721 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6781 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     6841 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     6901 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     6961 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7021 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7081 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//