LOCUS       RC206377                8199 bp    DNA     circular  SYN 
DEFINITION  PDGFRB (Myc-DDK-tagged)-Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB)
ACCESSION   NM_002609
VERSION     NM_002609.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4356..4447 
                     /label="Myc-DDK"  
     misc_feature    4477..5062 
                     /label="PolyA signal"  
     misc_feature    4534..4553 
                     /label="Restriction Enzyme"  
     misc_feature    5338..6010 
                     /label="ColE1"  
     misc_feature    6440..7234 
                     /label="Kan/Neo"  
     misc_feature    7269..7571 
                     /label="SV40 ori"  
     misc_feature    7657..8134 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4338..4344 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4338 
                     /label="PDGFRB"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGCTTCCG GGTGCGATGC CAGCTCTGGC CCTCAAAGGC GAGCTGCTGT 
     1081 TGCTGTCTCT CCTGTTACTT CTGGAACCAC AGATCTCTCA GGGCCTGGTC GTCACACCCC 
     1141 CGGGGCCAGA GCTTGTCCTC AATGTCTCCA GCACCTTCGT TCTGACCTGC TCGGGTTCAG 
     1201 CTCCGGTGGT GTGGGAACGG ATGTCCCAGG AGCCCCCACA GGAAATGGCC AAGGCCCAGG 
     1261 ATGGCACCTT CTCCAGCGTG CTCACACTGA CCAACCTCAC TGGGCTAGAC ACGGGAGAAT 
     1321 ACTTTTGCAC CCACAATGAC TCCCGTGGAC TGGAGACCGA TGAGCGGAAA CGGCTCTACA 
     1381 TCTTTGTGCC AGATCCCACC GTGGGCTTCC TCCCTAATGA TGCCGAGGAA CTATTCATCT 
     1441 TTCTCACGGA AATAACTGAG ATCACCATTC CATGCCGAGT AACAGACCCA CAGCTGGTGG 
     1501 TGACACTGCA CGAGAAGAAA GGGGACGTTG CACTGCCTGT CCCCTATGAT CACCAACGTG 
     1561 GCTTTTTTGG TATCTTTGAG GACAGAAGCT ACATCTGCAA AACCACCATT GGGGACAGGG 
     1621 AGGTGGATTC TGATGCCTAC TATGTCTACA GACTCCAGGT GTCATCCATC AACGTCTCTG 
     1681 TGAACGCAGT GCAGACTGTG GTCCGCCAGG GTGAGAACAT CACCCTCATG TGCATTGTGA 
     1741 TCGGGAATGA GGTGGTCAAC TTCGAGTGGA CATACCCCCG CAAAGAAAGT GGGCGGCTGG 
     1801 TGGAGCCGGT GACTGACTTC CTCTTGGATA TGCCTTACCA CATCCGCTCC ATCCTGCACA 
     1861 TCCCCAGTGC CGAGTTAGAA GACTCGGGGA CCTACACCTG CAATGTGACG GAGAGTGTGA 
     1921 ATGACCATCA GGATGAAAAG GCCATCAACA TCACCGTGGT TGAGAGCGGC TACGTGCGGC 
     1981 TCCTGGGAGA GGTGGGCACA CTACAATTTG CTGAGCTGCA TCGGAGCCGG ACACTGCAGG 
     2041 TAGTGTTCGA GGCCTACCCA CCGCCCACTG TCCTGTGGTT CAAAGACAAC CGCACCCTGG 
     2101 GCGACTCCAG CGCTGGCGAA ATCGCCCTGT CCACGCGCAA CGTGTCGGAG ACCCGGTATG 
     2161 TGTCAGAGCT GACACTGGTT CGCGTGAAGG TGGCAGAGGC TGGCCACTAC ACCATGCGGG 
     2221 CCTTCCATGA GGATGCTGAG GTCCAGCTCT CCTTCCAGCT ACAGATCAAT GTCCCTGTCC 
     2281 GAGTGCTGGA GCTAAGTGAG AGCCACCCTG ACAGTGGGGA ACAGACAGTC CGCTGTCGTG 
     2341 GCCGGGGCAT GCCCCAGCCG AACATCATCT GGTCTGCCTG CAGAGACCTC AAAAGGTGTC 
     2401 CACGTGAGCT GCCGCCCACG CTGCTGGGGA ACAGTTCCGA AGAGGAGAGC CAGCTGGAGA 
     2461 CTAACGTGAC GTACTGGGAG GAGGAGCAGG AGTTTGAGGT GGTGAGCACA CTGCGTCTGC 
     2521 AGCACGTGGA TCGGCCACTG TCGGTGCGCT GCACGCTGCG CAACGCTGTG GGCCAGGACA 
     2581 CGCAGGAGGT CATCGTGGTG CCACACTCCT TGCCCTTTAA GGTGGTGGTG ATCTCAGCCA 
     2641 TCCTGGCCCT GGTGGTGCTC ACCATCATCT CCCTTATCAT CCTCATCATG CTTTGGCAGA 
     2701 AGAAGCCACG TTACGAGATC CGATGGAAGG TGATTGAGTC TGTGAGCTCT GACGGCCATG 
     2761 AGTACATCTA CGTGGACCCC ATGCAGCTGC CCTATGACTC CACGTGGGAG CTGCCGCGGG 
     2821 ACCAGCTTGT GCTGGGACGC ACCCTCGGCT CTGGGGCCTT TGGGCAGGTG GTGGAGGCCA 
     2881 CGGCTCATGG CCTGAGCCAT TCTCAGGCCA CGATGAAAGT GGCCGTCAAG ATGCTTAAAT 
     2941 CCACAGCCCG CAGCAGTGAG AAGCAAGCCC TTATGTCGGA GCTGAAGATC ATGAGTCACC 
     3001 TTGGGCCCCA CCTGAACGTG GTCAACCTGT TGGGGGCCTG CACCAAAGGA GGACCCATCT 
     3061 ATATCATCAC TGAGTACTGC CGCTACGGAG ACCTGGTGGA CTACCTGCAC CGCAACAAAC 
     3121 ACACCTTCCT GCAGCACCAC TCCGACAAGC GCCGCCCGCC CAGCGCGGAG CTCTACAGCA 
     3181 ATGCTCTGCC CGTTGGGCTC CCCCTGCCCA GCCATGTGTC CTTGACCGGG GAGAGCGACG 
     3241 GTGGCTACAT GGACATGAGC AAGGACGAGT CGGTGGACTA TGTGCCCATG CTGGACATGA 
     3301 AAGGAGACGT CAAATATGCA GACATCGAGT CCTCCAACTA CATGGCCCCT TACGATAACT 
     3361 ACGTTCCCTC TGCCCCTGAG AGGACCTGCC GAGCAACTTT GATCAACGAG TCTCCAGTGC 
     3421 TAAGCTACAT GGACCTCGTG GGCTTCAGCT ACCAGGTGGC CAATGGCATG GAGTTTCTGG 
     3481 CCTCCAAGAA CTGCGTCCAC AGAGACCTGG CGGCTAGGAA CGTGCTCATC TGTGAAGGCA 
     3541 AGCTGGTCAA GATCTGTGAC TTTGGCCTGG CTCGAGACAT CATGCGGGAC TCGAATTACA 
     3601 TCTCCAAAGG CAGCACCTTT TTGCCTTTAA AGTGGATGGC TCCGGAGAGC ATCTTCAACA 
     3661 GCCTCTACAC CACCCTGAGC GACGTGTGGT CCTTCGGGAT CCTGCTCTGG GAGATCTTCA 
     3721 CCTTGGGTGG CACCCCTTAC CCAGAGCTGC CCATGAACGA GCAGTTCTAC AATGCCATCA 
     3781 AACGGGGTTA CCGCATGGCC CAGCCTGCCC ATGCCTCCGA CGAGATCTAT GAGATCATGC 
     3841 AGAAGTGCTG GGAAGAGAAG TTTGAGATTC GGCCCCCCTT CTCCCAGCTG GTGCTGCTTC 
     3901 TCGAGAGACT GTTGGGCGAA GGTTACAAAA AGAAGTACCA GCAGGTGGAT GAGGAGTTTC 
     3961 TGAGGAGTGA CCACCCAGCC ATCCTTCGGT CCCAGGCCCG CTTGCCTGGG TTCCATGGCC 
     4021 TCCGATCTCC CCTGGACACC AGCTCCGTCC TCTATACTGC CGTGCAGCCC AATGAGGGTG 
     4081 ACAACGACTA TATCATCCCC CTGCCTGACC CCAAACCCGA GGTTGCTGAC GAGGGCCCAC 
     4141 TGGAGGGTTC CCCCAGCCTA GCCAGCTCCA CCCTGAATGA AGTCAACACC TCCTCAACCA 
     4201 TCTCCTGTGA CAGCCCCCTG GAGCCCCAGG ACGAACCAGA GCCAGAGCCC CAGCTTGAGC 
     4261 TCCAGGTGGA GCCGGAGCCA GAGCTGGAAC AGTTGCCGGA TTCGGGGTGC CCTGCGCCTC 
     4321 GGGCGGAAGC AGAGGATAGC TTCCTGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4381 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4441 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4501 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4561 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4621 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4681 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4741 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4801 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4861 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4921 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4981 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5041 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5101 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5161 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5221 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5281 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5341 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5401 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5461 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5521 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5581 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5641 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5701 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5761 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5821 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5881 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5941 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6001 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6061 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6121 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6181 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6241 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6301 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6361 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6421 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6481 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6541 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6601 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6661 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6721 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6781 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6841 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6901 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6961 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7021 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7081 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7141 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7201 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7261 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7321 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7381 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7441 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7501 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7561 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7621 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7681 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7741 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7801 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7861 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7921 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7981 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8041 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8101 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8161 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//