LOCUS       RC206284L1                8928 bp    DNA     circular  SYN 
DEFINITION  DNM1 (Myc-DDK-tagged)-Human dynamin 1 (DNM1), transcript variant 2
ACCESSION   NM_001005336
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7219..7399 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8127..8786 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7558..8126 
                     /label="ColE1"  
     misc_feature    6094..6114 
                     /label="LR50 primer"  
     misc_feature    5891..5962 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6103..6694 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5873..5879 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5873 
                     /label="DNM1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGCAACCGC GGCATGGAAG ATCTCATCCC 
     3361 GCTGGTCAAC CGGCTGCAAG ACGCCTTCTC TGCCATCGGC CAGAACGCGG ACCTCGACCT 
     3421 GCCGCAGATC GCTGTGGTGG GCGGCCAGAG CGCCGGCAAG AGCTCGGTGC TCGAGAATTT 
     3481 CGTAGGCAGG GACTTCTTGC CTCGAGGATC TGGCATTGTC ACCCGACGTC CCCTGGTCTT 
     3541 GCAGCTGGTC AATGCAACCA CAGAATATGC CGAGTTCCTG CACTGCAAGG GAAAGAAATT 
     3601 CACCGACTTC GAGGAGGTGC GCCTTGAGAT CGAGGCCGAG ACCGACAGGG TCACCGGCAC 
     3661 CAACAAGGGC ATCTCGCCGG TGCCTATCAA CCTCCGCGTC TACTCGCCGC ACGTGCTGAA 
     3721 CCTGACCCTG GTGGACCTGC CCGGAATGAC CAAGGTCCCG GTGGGGGACC AACCTCCCGA 
     3781 CATCGAGTTC CAGATCCGAG ACATGCTTAT GCAGTTTGTC ACCAAGGAGA ACTGCCTCAT 
     3841 CCTGGCCGTG TCCCCCGCCA ACTCTGACCT GGCCAATTCT GACGCCCTCG AGGTCGCCAA 
     3901 GGAGGTGGAC CCCCAGGGCC AGCGCACCAT CGGGGTCATC ACCAAGCTGG ACCTGATGGA 
     3961 CGAGGGCACA GATGCCCGTG ATGTGCTGGA GAACAAGCTG CTCCCCCTGC GCAGAGGCTA 
     4021 CATTGGAGTG GTGAACCGGA GCCAGAAGGA CATTGATGGC AAGAAGGACA TTACCGCCGC 
     4081 CTTGGCTGCT GAACGAAAGT TCTTCCTCTC CCATCCATCT TATCGCCACT TGGCTGACCG 
     4141 TATGGGCACG CCCTACCTGC AGAAGGTCCT CAATCAGCAA CTGACGGACC ACATCCGGGA 
     4201 CACACTGCCG GGGCTGCGGA ACAAGCTGCA GAGCCAGCTA CTGTCCATTG AGAAGGAGGT 
     4261 GGAGGAATAC AAGAACTTCC GCCCTGATGA CCCAGCTCGC AAGACCAAGG CCCTGCTGCA 
     4321 GATGGTCCAG CAGTTCGCCG TAGACTTTGA GAAGCGCATT GAGGGCTCAG GAGATCAGAT 
     4381 CGACACCTAC GAACTGTCAG GGGGAGCCCG CATTAACCGA ATCTTCCACG AGCGCTTCCC 
     4441 TTTCGAGCTG GTCAAGATGG AGTTTGATGA GAAGGAACTC CGAAGGGAGA TCAGCTATGC 
     4501 TATCAAGAAT ATCCATGGCA TTAGAACGGG GCTGTTTACC CCAGACATGG CCTTTGAGAC 
     4561 CATTGTGAAA AAGCAGGTGA AGAAGATCCG AGAACCGTGT CTCAAGTGTG TGGACATGGT 
     4621 TATCTCGGAG CTAATCAGCA CCGTTAGACA GTGCACCAAG AAGCTCCAGC AGTACCCGCG 
     4681 GCTACGGGAG GAGATGGAGC GCATCGTGAC CACCCACATC CGGGAGCGCG AGGGCCGCAC 
     4741 TAAGGAGCAG GTCATGCTTC TCATCGATAT CGAGCTGGCT TACATGAACA CCAACCATGA 
     4801 GGACTTCATA GGCTTTGCCA ATGCTCAGCA GAGGAGCAAC CAGATGAACA AGAAGAAGAC 
     4861 TTCAGGGAAC CAGGATGAGA TTCTGGTCAT CCGCAAGGGC TGGCTGACTA TCAATAATAT 
     4921 TGGCATCATG AAAGGGGGCT CCAAGGAGTA CTGGTTTGTG CTGACTGCTG AGAATCTGTC 
     4981 CTGGTACAAG GATGATGAGG AGAAAGAGAA GAAATACATG CTGTCTGTGG ACAACCTCAA 
     5041 GCTGCGGGAC GTGGAGAAGG GCTTTATGTC GAGCAAGCAT ATCTTTGCCC TCTTTAACAC 
     5101 GGAGCAGAGG AATGTCTACA AGGATTATCG GCAGCTGGAG CTAGCCTGTG AGACACAGGA 
     5161 GGAGGTGGAC AGCTGGAAGG CCTCCTTCCT GAGGGCTGGC GTGTACCCTG AGCGTGTTGG 
     5221 GGACAAAGAG AAAGCCAGCG AGACCGAGGA GAATGGCTCC GACAGCTTCA TGCATTCCAT 
     5281 GGACCCACAG CTGGAACGGC AAGTGGAGAC CATCCGGAAT CTTGTGGACT CATACATGGC 
     5341 CATTGTCAAC AAGACCGTGA GGGACCTCAT GCCCAAGACC ATCATGCACC TCATGATTAA 
     5401 CAATACCAAG GAGTTCATCT TCTCGGAGCT GCTGGCCAAC CTGTACTCGT GTGGGGACCA 
     5461 GAACACGCTG ATGGAGGAGT CGGCGGAGCA GGCACAGCGG CGCGACGAGA TGCTGCGCAT 
     5521 GTACCACGCA CTGAAGGAGG CGCTCAGCAT CATCGGCGAC ATCAACACGA CCACCGTCAG 
     5581 CACGCCCATG CCCCCGCCCG TGGACGACTC CTGGCTGCAG GTGCAGAGCG TACCGGCCGG 
     5641 ACGCAGGTCG CCCACGTCCA GCCCCACGCC GCAGCGCCGA GCCCCCGCCG TGCCCCCAGC 
     5701 CCGGCCCGGG TCGCGGGGCC CTGCTCCTGG GCCTCCGCCT GCTGGGTCCG CCATGGGGGG 
     5761 GGCGCCCCCC GTGCCCTCCA GGCCGGGGGC TTCCCCTGAC CCTTTCGGCC CTCCCCCTCA 
     5821 GGTGCCCTCG CGCCCCAACC GCGCCCCGCC CGGGGTCCCC AGAATCACTA TCAGTGACCC 
     5881 CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA 
     5941 TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCTGTACAA 
     6001 GTAGGATTCG TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATACAT 
     6061 GTTTAAGGGT TCCGGTTCCA CTAGGTACAA TTCGATATCA AGCTTATCGA TAATCAACCT 
     6121 CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG 
     6181 CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC 
     6241 ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT 
     6301 GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC 
     6361 ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG 
     6421 GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT 
     6481 GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT 
     6541 GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG 
     6601 GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC 
     6661 CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGACCTCGA 
     6721 TCGAGACCTA GAAAAACATG GAGCAATCAC AAGTAGCAAT ACAGCAGCTA CCAATGCTGA 
     6781 TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT 
     6841 ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA 
     6901 GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAGACAA GATATCCTTG ATCTGTGGAT 
     6961 CTACCACACA CAAGGCTACT TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG 
     7021 ATATCCACTG ACCTTTGGAT GGTGCTACAA GCTAGTACCA GTTGAGCAAG AGAAGGTAGA 
     7081 AGAAGCCAAT GAAGGAGAGA ACACCCGCTT GTTACACCCT GTGAGCCTGC ATGGGATGGA 
     7141 TGACCCGGAG AGAGAAGTAT TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT 
     7201 GGCCCGAGAG CTGCATCCGG ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG 
     7261 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT 
     7321 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC 
     7381 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     7441 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     7501 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     7561 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     7621 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     7681 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     7741 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     7801 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     7861 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     7921 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     7981 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     8041 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     8101 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGATTACG CCCCGCCCTG CCACTCATCG 
     8161 CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA 
     8221 TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG 
     8281 GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA 
     8341 CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG 
     8401 GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA 
     8461 TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG 
     8521 TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAC 
     8581 TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC 
     8641 TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG 
     8701 GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA 
     8761 TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATACTCT TCCTTTTTCA ATATTATTGA 
     8821 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8881 AAACAAATAG GGGTCCCGCG CACATTTCCC CGAAAAGTGC CACCTGAC
//