LOCUS       RC206228                7278 bp    DNA     circular  SYN 
DEFINITION  BEND2 (Myc-DDK-tagged)-Human BEN domain containing 2 (BEND2), transcript variant 1
ACCESSION   NM_153346
VERSION     NM_153346.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3435..3526 
                     /label="Myc-DDK"  
     misc_feature    3556..4141 
                     /label="PolyA signal"  
     misc_feature    3613..3632 
                     /label="Restriction Enzyme"  
     misc_feature    4417..5089 
                     /label="ColE1"  
     misc_feature    5519..6313 
                     /label="Kan/Neo"  
     misc_feature    6348..6650 
                     /label="SV40 ori"  
     misc_feature    6736..7213 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3417..3423 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3417 
                     /label="BEND2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGAGAGG ACCCAGGAAC AGGATTTTGT TATTATAACT GTCGACGACA 
     1081 GTGATGATAA CAATGATTGC AGTATTGAGA TGGTGGAAGT TTCTGAAACA GCAGATAATT 
     1141 CCACTAATGA CATAGCAGAT GATTCCACTT ATGTCACAGC AGATAATCCC ACTGATGACA 
     1201 CAGCCACACA ACCAAATTTT CCAGGCGGCA ATGATGGCCA TCACCGTCCA CTCCAAATGT 
     1261 CATATGGGTC TGGCTCTGTC ACCCAGGCTG GAGTGCAGTG GCATGATCAC AGCTCACTGC 
     1321 AGCCTCAACC TCTTGGACTC AAGCAATTCT TCCACCTCAG CCTTCCCAGT AGCTGGGATG 
     1381 ACAGGCGCAC GCCACCATGC CCAGTAGCCC ATGGTGACCA AATAGTTTCA CAGATAAACC 
     1441 ACCCAGTACA TTTAAGAAGA TACAGTTACA ACTCAGAGGA AGTGGATTTT CCAAAAAGAG 
     1501 GAAGATTCTA TACTCCAGAG GTACAGTCTA GCATATCACC ACCAGCGGAA AGGCAGGAAA 
     1561 CCCATGCCTG GGCCAGCCCT GCTGTAACAT CTCTTGAGTC AGCAGCATGT CATGAACTGC 
     1621 AGGAAGCAGA CCTCAGTGAG AGTTTATCAT ATCCCAGAAT TGTCTCCTCA AGTTCATTAC 
     1681 AGCAGTATGT CGCACAAGGT GGCTCATTTC CTTGTTTCGG TATGCCATGG AACTTTATCA 
     1741 GTGGAGGTGC TGAAAGTACC AATGCTGTCA TCTCATTTGC CAATGCTACT ACAGCAGTAC 
     1801 CTATGGCAGT TCTGTCACGA AGAGAATCCA GTCTGGCAAA TAACCCTGGT GTGGTGAATT 
     1861 ACTCTGCTCT ACCAGAAAAT GAAAATGTGG GCCCAGGTAG AGCCTTGTCA TCTTTCTGCT 
     1921 TCCATCCCAA TTTGGAAATG CCAGAAAGAC CAGCAAACAG CAGTAAAAAC AGCACTGAGA 
     1981 CAGCGAATTA TCCAACTTTA ATGGGAAATT ACAATGGCCA AAATACTGCC TCTTTATCTG 
     2041 TCTTCATCCC TCCCTATTTT GCTGAGAAAA TTATACTTAC TGAAATGCCA GGAACAACAG 
     2101 AAACCAACGT GGAAAATAAC TCTCAGACAG TGTATTACCC AGCTTTATCG GGAAATACGA 
     2161 GTGCCCCATA TCCAGCCTCT TCATATCTTC CCATCACTTC TAATTTTGAA TCTGGCCCAC 
     2221 AAATGAGTTA TGGGACAATG AGTTACTCAA CTGAAATGAA AAATAACTGT GACCAAGATG 
     2281 ATGCTTCAGC ATCTGCCTGC CTCACTCCCG ATTTTGCACT GTTACCTCTG AATATTTTGG 
     2341 TAAAAGTAGA CACCAACACG GAAAACAGCG TCAACACAAT GAATCGCTCA ACTTTATTGG 
     2401 ACAGTGACAG TGGCCAGGAT TCTTCCTCAT CATCTGTCTG TATCCCTCCC AAGTATGGCT 
     2461 ATCTTGGTGA TCCAAAAAGA AATGTCAGAG TACTTAAAAT CCATTTGCTG GCCGTACAAA 
     2521 ATATGGCCAA ACCTAAGCAA GCAGCCTGCT ACTTGGTTCG TATTTTGTTC TCCAAGGAAA 
     2581 TCCTGATTAG CAGCTCAGTG GATATCCATT TGAAAGACAG CCAATCCCTC GACCCGAACA 
     2641 AAATGGCTGC ATTGAGAGAA TATCTGGCAA CAACTTTTCC CACCTGTGAT TTGCATGAAC 
     2701 ATGGAAAAGA CTGGCAGGAC TGTATTTCTG GTATCAATTC TATGATCTAC TGTTTATGTT 
     2761 CTGAAGGCAA GAGTACTCCA AAAACTGTTC GCAAAAATAA AAAACGTACC AACCGTGTTG 
     2821 CATCGGCATC TGCAGATAGA AATGACCAAA GGGGCAGAGA TGGTGGTGAA GGCTGTTCTT 
     2881 GGATGTTTCA GCCAATGAAT AACTCCAAAA TGAGAGTAAA GAGGAACTTG CAGCCAAACA 
     2941 GTAATGCTAT CCCTGAAGGA ATGCGAGAAC CTTCCACTGA TAATCCAGAG GAACCTGGTG 
     3001 AAGCATGGAG CTATTTTGGA AGACCATGGA GAAATATACG GATGCCATGT TCAGTACTGA 
     3061 CTTTGGCAAA AACTAAGTCT TGCGCAAGCC TGTCGGCTAG ATACCTTATT CAGAAACTCT 
     3121 TCACAAAAGA TGTCCTGGTC CAAAGTAACG TCTATGGCAA TCTGAAGCAT GGCCTGTGTG 
     3181 CCCTTGACCC CAATAAGATT AGTGCTCTCC GAGAGTTCCT CCAAGAAAAC TACCCAATTT 
     3241 GTGATCTCTC GGAAAATGGA AGAGACTGGA AGTCGTGTGT GACCTCCATC AACAGCGGTA 
     3301 TCCGTAGCCT TAGACATGAC GTCAGAAGGG CTGAAGCCAG GTCTCAGTCG CTTCCAGCAG 
     3361 TGACCCCTCC AGAGCTGGAG CAGGAGTCAA AGCCAGGAGA TCCCGACGCC ACTGACCCAA 
     3421 GCACCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3481 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3541 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3601 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3661 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3721 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3781 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3841 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3901 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3961 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4021 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4081 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4141 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4201 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4261 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4321 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4381 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4441 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4501 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4561 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4621 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4681 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4741 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4801 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4861 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4921 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4981 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5041 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5101 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5161 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5221 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5281 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5341 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5401 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5461 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5521 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5581 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5641 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5701 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5761 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5821 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5881 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5941 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6001 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6061 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6121 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6181 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6241 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6301 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6361 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6421 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6481 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6541 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6601 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6661 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6721 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6781 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6841 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6901 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6961 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7021 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7081 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7141 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7201 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7261 AAATTTAACG CGAATTTT
//