LOCUS       RC206004                8094 bp    DNA     circular  SYN 
DEFINITION  XPO1 (Myc-DDK-tagged)-Human exportin 1 (CRM1 homolog, yeast) (XPO1)
ACCESSION   NM_003400
VERSION     NM_003400.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4251..4342 
                     /label="Myc-DDK"  
     misc_feature    4372..4957 
                     /label="PolyA signal"  
     misc_feature    4429..4448 
                     /label="Restriction Enzyme"  
     misc_feature    5233..5905 
                     /label="ColE1"  
     misc_feature    6335..7129 
                     /label="Kan/Neo"  
     misc_feature    7164..7466 
                     /label="SV40 ori"  
     misc_feature    7552..8029 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4233..4239 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4233 
                     /label="XPO1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGCAATT ATGACAATGT TAGCAGACCA TGCAGCTCGT CAGCTGCTTG 
     1081 ATTTCAGCCA AAAACTGGAT ATCAACTTAT TAGATAATGT GGTGAATTGC TTATACCATG 
     1141 GAGAAGGAGC CCAGCAAAGA ATGGCTCAAG AAGTACTGAC ACATTTAAAG GAGCATCCTG 
     1201 ATGCTTGGAC AAGAGTCGAC ACAATTTTGG AATTTTCTCA GAATATGAAT ACGAAATACT 
     1261 ATGGACTACA AATTTTGGAA AATGTGATAA AAACAAGGTG GAAGATTCTT CCAAGGAACC 
     1321 AGTGCGAAGG AATAAAAAAA TACGTTGTTG GCCTCATTAT CAAGACGTCA TCTGACCCAA 
     1381 CTTGTGTAGA GAAAGAAAAG GTGTATATCG GAAAATTAAA TATGATCCTT GTTCAGATAC 
     1441 TGAAACAAGA ATGGCCCAAA CATTGGCCAA CTTTTATCAG TGATATTGTT GGAGCAAGTA 
     1501 GGACCAGCGA AAGTCTCTGT CAAAATAATA TGGTGATTCT TAAACTCTTG AGTGAAGAAG 
     1561 TATTTGATTT CTCTAGTGGA CAGATAACCC AAGTCAAATC TAAGCATTTA AAAGACAGCA 
     1621 TGTGCAATGA ATTCTCACAG ATATTTCAAC TGTGTCAGTT TGTAATGGAA AATTCTCAAA 
     1681 ATGCTCCACT TGTACATGCA ACCTTGGAAA CATTGCTCAG ATTTCTGAAC TGGATTCCCC 
     1741 TGGGATATAT TTTTGAGACC AAATTAATCA GCACATTGAT TTATAAGTTC CTGAATGTTC 
     1801 CAATGTTTCG AAATGTCTCT CTGAAGTGCC TCACTGAGAT TGCTGGTGTG AGTGTAAGCC 
     1861 AATATGAAGA ACAATTTGTA ACACTATTTA CTCTGACAAT GATGCAACTA AAGCAGATGC 
     1921 TTCCTTTAAA TACCAATATT CGACTTGCGT ACTCAAATGG AAAAGATGAT GAACAGAACT 
     1981 TCATTCAAAA TCTCAGTTTG TTTCTCTGCA CCTTTCTTAA GGAACATGAT CAACTTATAG 
     2041 AAAAAAGATT AAATCTCAGG GAAACTCTTA TGGAGGCCCT TCATTATATG TTGTTGGTAT 
     2101 CTGAAGTAGA AGAAACTGAA ATCTTTAAAA TTTGTCTTGA ATACTGGAAT CATTTGGCTG 
     2161 CTGAACTCTA TAGAGAGAGT CCATTCTCTA CATCTGCCTC TCCGTTGCTT TCTGGAAGTC 
     2221 AACATTTTGA TGTTCCTCCC AGGAGACAGC TATATTTGCC CATGTTATTC AAGGTCCGTT 
     2281 TATTAATGGT TAGTCGAATG GCTAAACCAG AGGAAGTATT GGTTGTAGAG AATGATCAAG 
     2341 GAGAAGTTGT GAGAGAATTC ATGAAGGATA CAGATTCCAT AAATTTGTAT AAGAATATGA 
     2401 GGGAAACATT GGTTTATCTT ACTCATCTGG ATTATGTAGA TACAGAAAGA ATAATGACAG 
     2461 AGAAGCTTCA CAATCAAGTG AATGGTACAG AGTGGTCATG GAAAAATTTG AATACATTGT 
     2521 GTTGGGCAAT AGGCTCCATT AGTGGAGCAA TGCATGAAGA GGACGAAAAA CGATTTCTTG 
     2581 TTACTGTTAT AAAGGATCTA TTAGGATTAT GTGAACAGAA AAGAGGCAAA GATAATAAAG 
     2641 CTATTATTGC ATCAAATATC ATGTACATAG TAGGTCAATA CCCACGTTTT TTGAGAGCTC 
     2701 ACTGGAAATT TCTGAAGACT GTAGTTAACA AGCTGTTCGA ATTCATGCAT GAGACCCATG 
     2761 ATGGAGTCCA GGATATGGCT TGTGATACTT TCATTAAAAT AGCCCAAAAA TGCCGCAGGC 
     2821 ATTTCGTTCA GGTTCAGGTT GGAGAAGTGA TGCCATTTAT TGATGAAATT TTGAACAACA 
     2881 TTAACACTAT TATTTGTGAT CTTCAGCCTC AACAGGTTCA TACGTTTTAT GAAGCTGTGG 
     2941 GGTACATGAT TGGTGCACAA ACAGATCAAA CAGTACAAGA ACACTTGATA GAAAAGTACA 
     3001 TGTTACTCCC TAATCAAGTG TGGGATAGTA TAATCCAGCA GGCAACCAAA AATGTGGATA 
     3061 TACTGAAAGA TCCTGAAACA GTCAAGCAGC TTGGTAGCAT TTTGAAAACA AATGTGAGAG 
     3121 CCTGCAAAGC TGTTGGACAC CCCTTTGTAA TTCAGCTTGG AAGAATTTAT TTAGATATGC 
     3181 TTAATGTATA CAAGTGCCTC AGTGAAAATA TTTCTGCAGC TATCCAAGCT AATGGTGAAA 
     3241 TGGTTACAAA GCAACCATTG ATTAGAAGTA TGCGAACTGT AAAAAGGGAA ACTTTAAAGT 
     3301 TAATATCTGG TTGGGTGAGC CGATCCAATG ATCCACAGAT GGTCGCTGAA AATTTTGTTC 
     3361 CCCCTCTGTT GGATGCAGTT CTCATTGATT ATCAGAGAAA TGTCCCAGCT GCTAGAGAAC 
     3421 CAGAAGTGCT TAGTACTATG GCCATAATTG TCAACAAGTT AGGGGGACAT ATAACAGCTG 
     3481 AAATACCTCA AATATTTGAT GCTGTTTTTG AATGCACATT GAATATGATA AATAAGGACT 
     3541 TTGAAGAATA TCCTGAACAT AGAACGAACT TTTTCTTACT ACTTCAGGCT GTCAATTCTC 
     3601 ATTGTTTCCC AGCATTCCTT GCTATTCCAC CTACACAGTT TAAACTTGTT TTGGATTCCA 
     3661 TCATTTGGGC TTTCAAACAT ACTATGAGGA ATGTCGCAGA TACGGGCTTA CAGATACTTT 
     3721 TTACACTCTT ACAAAATGTT GCACAAGAAG AAGCTGCAGC TCAGAGTTTT TATCAAACTT 
     3781 ATTTTTGTGA TATTCTCCAG CATATCTTTT CTGTTGTGAC AGACACTTCA CATACTGCTG 
     3841 GTTTAACAAT GCATGCATCA ATTCTTGCAT ATATGTTTAA TTTGGTTGAA GAAGGAAAAA 
     3901 TAAGTACATC ATTAAATCCT GGAAATCCAG TTAACAACCA AATCTTTCTT CAGGAATATG 
     3961 TGGCTAATCT CCTTAAGTCG GCCTTCCCTC ACCTACAAGA TGCTCAAGTA AAGCTCTTTG 
     4021 TGACAGGGCT TTTCAGCTTA AATCAAGATA TTCCTGCTTT CAAGGAACAT TTAAGAGATT 
     4081 TCCTAGTTCA AATAAAGGAA TTTGCAGGTG AAGACACTTC TGATTTGTTT TTGGAAGAGA 
     4141 GAGAAATAGC CCTACGGCAG GCTGATGAAG AGAAACATAA ACGTCAAATG TCTGTCCCTG 
     4201 GCATCTTTAA TCCACATGAG ATTCCAGAAG AAATGTGTGA TACGCGTACG CGGCCGCTCG 
     4261 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4321 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4381 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4441 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4501 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4561 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4621 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4681 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4741 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4801 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4861 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4921 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4981 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5041 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5101 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5161 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5221 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5281 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5341 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5401 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5461 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5521 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5581 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5641 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5701 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5761 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5821 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5881 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5941 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6001 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6061 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6121 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6181 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6241 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6301 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6361 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6421 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6481 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6541 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6601 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6661 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6721 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6781 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6841 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6901 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6961 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7021 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7081 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7141 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7201 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7261 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7321 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7381 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7441 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7501 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7561 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7621 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7681 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7741 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7801 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7861 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7921 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7981 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8041 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//