LOCUS       RC205643                7533 bp    DNA     circular  SYN 
DEFINITION  CHERP (Myc-DDK-tagged)-Human calcium homeostasis endoplasmic reticulum protein (CHERP)
ACCESSION   NM_006387
VERSION     NM_006387.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3690..3781 
                     /label="Myc-DDK"  
     misc_feature    3811..4396 
                     /label="PolyA signal"  
     misc_feature    3868..3887 
                     /label="Restriction Enzyme"  
     misc_feature    4672..5344 
                     /label="ColE1"  
     misc_feature    5774..6568 
                     /label="Kan/Neo"  
     misc_feature    6603..6905 
                     /label="SV40 ori"  
     misc_feature    6991..7468 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3672..3678 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3672 
                     /label="CHERP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTATGGAG AAGCAGAAGG ACAACCCCAA ATTCTCGTTT CTTTTCGGAG 
     1081 GCGAATTCTA CAGTTACTAC AAGTGCAAGC TGGCGCTGGA GCAGCAGCAG CTCATCTGCA 
     1141 AGCAGCAGAC CCCGGAGCTG GAGCCAGCCG CCACCATGCC ACCCCTGCCA CAGCCCCCGC 
     1201 TGGCCCCCGC CGCGCCCATC CCGCCGGCCC AGGGCGCGCC ATCCATGGAC GAGCTCATCC 
     1261 AGCAGAGCCA GTGGAACCTC CAGCAGCAGG AGCAGCACTT GCTGGCGCTC AGACAGGAGC 
     1321 AAGTGACAGC GGCCGTGGCC CACGCGGTGG AGCAGCAGAT GCAGAAGCTT CTGGAGGAGA 
     1381 CCCAGCTAGA CATGAACGAG TTTGACAACC TCCTGCAGCC CATCATCGAC ACGTGCACCA 
     1441 AGGACGCCAT CTCGGCCGGG AAGAACTGGA TGTTCAGCAA TGCCAAGTCC CCGCCGCACT 
     1501 GTGAGCTGAT GGCCGGCCAC CTCCGGAACC GCATCACGGC TGATGGGGCA CACTTCGAGC 
     1561 TGCGGCTGCA CCTCATCTAC CTGATCAATG ACGTGCTGCA CCACTGCCAG CGCAAGCAGG 
     1621 CCCGGGAGCT GCTGGCCGCC CTGCAGAAGG TCGTGGTGCC CATCTACTGC ACCAGCTTCT 
     1681 TGGCCGTGGA GGAAGACAAG CAGCAGAAGA TCGCCCGGCT CCTGCAGCTC TGGGAGAAAA 
     1741 ACGGCTACTT CGATGACTCC ATCATTCAGC AGCTACAGAG CCCAGCCCTG GGGCTTGGTC 
     1801 AGTACCAGGC CACCCTCATC AACGAGTACT CCTCAGTGGT CCAGCCGGTG CAGCTGGCCT 
     1861 TCCAGCAGCA GATCCAGACC CTCAAGACGC AGCACGAGGA GTTTGTCACC AGCCTGGCCC 
     1921 AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCAGCTCCA GATGCCGCAG ATGGAGGCTG 
     1981 AAGTCAAGGC CACGCCTCCA CCGCCTGCTC CACCCCCGGC CCCAGCACCT GCCCCTGCCA 
     2041 TCCCGCCCAC CACCCAGCCT GATGACAGCA AGCCTCCCAT CCAGATGCCT GGCTCTTCAG 
     2101 AGTACGAAGC TCCAGGAGGG GTCCAGGATC CTGCAGCTGC CGGCCCCCGG GGCCCCGGGC 
     2161 CACACGACCA GATCCCACCA AACAAGCCCC CTTGGTTTGA CCAGCCTCAC CCCGTGGCTC 
     2221 CTTGGGGCCA GCAGCAGCCG CCAGAGCAGC CACCCTACCC GCACCACCAG GGCGGCCCAC 
     2281 CCCACTGCCC CCCCTGGAAC AACAGCCATG AGGGCATGTG GGGCGAGCAG CGCGGTGACC 
     2341 CCGGCTGGAA CGGCCAGCGC GACGCGCCCT GGAACAACCA GCCCGACGCC GCCTGGAACA 
     2401 GCCAGTTCGA GGGCCCCTGG AACAGCCAGC ACGAGCAGCC GCCCTGGGGC GGGGGCCAGC 
     2461 GCGAGCCACC CTTCCGCATG CAGCGGCCCC CACACTTCCG GGGGCCCTTC CCGCCCCACC 
     2521 AGCAGCACCC GCAGTTCAAC CAGCCTCCGC ACCCCCACAA CTTCAACCGC TTCCCGCCCC 
     2581 GCTTCATGCA GGACGACTTC CCGCCACGGC ACCCCTTCGA GCGGCCGCCC TATCCCCACC 
     2641 GCTTCGACTA CCCCCAGGGG GACTTCCCTG CCGAAATGGG GCCCCCTCAC CACCACCCTG 
     2701 GCCACCGCAT GCCTCATCCT GGCATCAACG AGCACCCGCC TTGGGCTGGA CCCCAGCACC 
     2761 CTGACTTCGG CCCTCCCCCC CATGGCTTCA ACGGGCAGCC CCCACACATG CGGCGACAGG 
     2821 GCCCGCCCCA CATCAACCAC GATGACCCCA GCCTGGTCCC CAATGTGCCC TACTTCGATC 
     2881 TCCCTGCTGG GCTGATGGCC CCCCTCGTGA AGCTGGAAGA TCACGAGTAC AAGCCTTTGG 
     2941 ACCCTAAAGA CATCCGCCTC CCACCCCCCA TGCCGCCCAG CGAGAGGCTG CTGGCTGCAG 
     3001 TGGAGGCCTT CTACAGCCCC CCGTCCCACG ACAGGCCCAG GAACAGTGAA GGCTGGGAGC 
     3061 AGAACGGCCT CTATGAGTTC TTCCGAGCAA AAATGCGGGC CCGGCGGAGG AAAGGCCAGG 
     3121 AGAAGAGGAA CAGCGGACCC TCGAGGTCTC GGAGCAGATC CAAGAGTCGA GGGCGTTCTT 
     3181 CCTCCCGCTC CAACTCAAGA TCCTCCAAGT CTTCAGGCTC GTACTCAAGG TCAAGGTCGC 
     3241 GCTCCTGCTC CCGTTCCTAC TCCCGCTCCA GATCTAGAAG TCGGAGCAGG TCGCGCTCCT 
     3301 CCAGAAGCCG CTCCCGGTCC CAGTCGCGGT CCCGGTCCAA GTCGTACTCC CCAGGAAGAA 
     3361 GACGCCGGTC ACGGTCCAGG AGCCCCACCC CGCCTTCCTC TGCTGGTCTG GGTTCTAATT 
     3421 CGGCGCCTCC CATTCCTGAC TCAAGGCTCG GAGAAGAGAA CAAAGGCCAT CAGATGCTGG 
     3481 TGAAGATGGG CTGGAGCGGC TCAGGCGGCC TCGGTGCGAA GGAGCAAGGG ATCCAGGACC 
     3541 CCATCAAGGG CGGGGACGTC CGGGATAAGT GGGACCAGTA TAAAGGCGTG GGCGTGGCTC 
     3601 TGGATGACCC CTATGAGAAC TACCGCAGGA ACAAGAGCTA CTCCTTCATC GCCCGCATGA 
     3661 AGGCCAGGGA CGAGTGTAAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3721 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3781 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3841 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3901 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3961 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4021 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4081 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4141 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4201 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4261 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4321 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4381 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4441 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4501 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4561 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4621 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4681 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4741 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4801 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4861 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4921 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4981 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5041 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5101 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5161 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5221 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5281 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5341 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5401 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5461 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5521 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5581 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5641 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5701 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5761 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5821 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5881 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5941 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6001 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6061 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6121 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6181 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6241 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6301 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6361 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6421 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6481 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6541 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6601 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6661 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6721 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6781 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6841 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6901 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6961 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7021 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7081 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7141 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7201 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7261 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7321 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7381 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7441 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7501 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//