LOCUS       RC205548                7497 bp    DNA     circular  SYN 
DEFINITION  DDHD1 (Myc-DDK-tagged)-Human DDHD domain containing 1 (DDHD1), transcript variant 1
ACCESSION   NM_030637
VERSION     NM_030637.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3654..3745 
                     /label="Myc-DDK"  
     misc_feature    3775..4360 
                     /label="PolyA signal"  
     misc_feature    3832..3851 
                     /label="Restriction Enzyme"  
     misc_feature    4636..5308 
                     /label="ColE1"  
     misc_feature    5738..6532 
                     /label="Kan/Neo"  
     misc_feature    6567..6869 
                     /label="SV40 ori"  
     misc_feature    6955..7432 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3636..3642 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3636 
                     /label="DDHD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATTACCCG GGCCGCGGGT CCCCACGGAG CCCCGAGCAT AACGGCCGAG 
     1081 GCGGCGGCGG CGGCGCCTGG GAGCTGGGCT CAGACGCGAG GCCAGCGTTC GGCGGCGGCG 
     1141 TCTGCTGCTT CGAGCACCTG CCCGGCGGGG ACCCGGACGA CGGCGACGTG CCCCTGGCCC 
     1201 TGCTGCGCGG GGAACCCGGG CTGCATTTGG CGCCGGGCAC CGACGACCAC AACCACCACC 
     1261 TCGCGCTGGA CCCCTGCCTC AGTGACGAGA ACTATGACTT CAGCTCCGCC GAGTCGGGCT 
     1321 CCTCGCTGCG CTACTACAGC GAGGGTGAGA GCGGCGGCGG CGGCAGCTCC TTGTCGCTGC 
     1381 ACCCGCCGCA GCAGCCTCCG CTGGTCCCGA CGAACTCGGG GGGCGGCGGC GCGACAGGAG 
     1441 GGTCCCCCGG GGAAAGGAAA CGTACCCGGC TTGGCGGCCC GGCGGCCCGG CACCGCTATG 
     1501 AGGTAGTGAC GGAGCTGGGC CCGGAGGAGG TACGCTGGTT CTACAAGGAG GACAAGAAGA 
     1561 CCTGGAAGCC CTTCATCGGC TACGACTCGC TCCGCATCGA GCTCGCCTTC CGGACCCTGC 
     1621 TGCAGACCAC GGGTGCCCGG CCCCAGGGCG GGGACCGGGA CGGCGACCAT GTGTGCTCCC 
     1681 CCACGGGCCC AGCCTCCAGT TCCGGAGAAG ATGACGATGA GGACCGCGCC TGCGGCTTCT 
     1741 GCCAGAGTAC GACGGGGCAC GAGCCGGAGA TGGTGGAGCT TGTGAACATC GAGCCTGTGT 
     1801 GCGTGCGGGG CGGCCTCTAC GAGGTGGATG TGACCCAAGG AGAGTGCTAC CCGGTGTACT 
     1861 GGAACCAGGC TGATAAAATA CCAGTAATGC GTGGACAGTG GTTTATTGAC GGCACTTGGC 
     1921 AGCCTCTAGA AGAGGAAGAA AGTAATTTAA TTGAGCAAGA ACATCTCAAT TGTTTTAGGG 
     1981 GCCAGCAGAT GCAGGAAAAT TTCGATATTG AAGTGTCAAA ATCCATAGAT GGAAAAGATG 
     2041 CTGTTCATAG TTTCAAGTTG AGTCGAAACC ATGTGGACTG GCACAGTGTG GATGAAGTAT 
     2101 ATCTTTATAG TGATGCAACA ACATCTAAAA TTGCAAGAAC AGTTACCCAA AAACTGGGAT 
     2161 TTTCTAAAGC ATCAAGTAGT GGTACCAGAC TTCATAGAGG TTATGTAGAA GAAGCCACAT 
     2221 TAGAAGACAA GCCATCACAG ACTACCCATA TTGTATTTGT TGTGCATGGC ATTGGGCAGA 
     2281 AAATGGACCA AGGAAGAATT ATCAAAAATA CAGCTATGAT GAGAGAAGCT GCAAGAAAAA 
     2341 TAGAAGAAAG GCATTTTTCC AACCATGCAA CACATGTTGA ATTTCTGCCT GTTGAGTGGC 
     2401 GGTCAAAACT TACTCTTGAT GGAGACACTG TTGATTCCAT TACTCCTGAC AAAGTACGAG 
     2461 GTTTAAGGGA TATGCTGAAC AGCAGTGCAA TGGACATAAT GTATTATACT AGTCCACTTT 
     2521 ATAGAGATGA ACTAGTTAAA GGCCTTCAGC AAGAGCTGAA TCGATTGTAT TCCCTTTTCT 
     2581 GTTCTCGGAA TCCAGACTTT GAAGAAAAAG GGGGTAAAGT CTCAATAGTA TCACATTCCT 
     2641 TGGGATGTGT AATTACTTAT GACATAATGA CTGGCTGGAA TCCAGTTCGG CTGTATGAAC 
     2701 AGTTGCTGCA AAAGGAAGAA GAGTTGCCTG ATGAACGATG GATGAGCTAT GAAGAACGAC 
     2761 ATCTTCTTGA TGAACTCTAT ATAACTAAAC GACGGCTGAA GGAAATAGAA GAACGGCTTC 
     2821 ACGGATTGAA AGCATCATCT ATGACACAAA CACCTGCCTT AAAATTTAAG GTTGAGAATT 
     2881 TCTTCTGTAT GGGATCCCCA TTAGCAGTTT TCTTGGCGTT GCGTGGCATC CGCCCAGGAA 
     2941 ATACTGGAAG TCAAGACCAT ATTTTGCCTA GAGAGATTTG TAACCGGTTA CTAAATATTT 
     3001 TTCATCCTAC AGATCCAGTG GCTTATAGAT TAGAACCATT AATACTGAAA CGCTACAGCA 
     3061 ACATTTCACC TGTCCAGATC CACTGGTACA ATACTTCAAA TCCTTTACCT TATGAACATA 
     3121 TGAAGCCAAG CTTTCTCAAC CCAGCTAAAG AACCTACCTC AGTTTCAGAG AATGAAGGCA 
     3181 TTTCAACCAT ACCAAGCCCT GTGACCTCAC CAGTTTTGTC CCGCCGACAC TATGGAGAAT 
     3241 CTATAACAAA TATAGGCAAA GCAAGCATAT TAGGGGCTGC TAGCATTGGA AAGGGACTTG 
     3301 GAGGAATGTT GTTCTCAAGA TTTGGACGTT CATCTACAAC ACAGTCATCT GAAACATCAA 
     3361 AAGACTCAAT GGAAGATGAG AAGAAGCCAG TTGCCTCACC TTCTGCTACC ACCGTAGGGA 
     3421 CACAGACCCT TCCACATAGC AGTTCTGGCT TCCTCGATTC TGCATTGGAG TTGGATCACA 
     3481 GGATTGATTT TGAACTCAGA GAAGGCCTTG TGGAGAGCCG CTATTGGTCA GCTGTCACGT 
     3541 CGCATACTGC CTATTGGTCA TCCTTGGATG TTGCCCTTTT TCTTTTAACC TTCATGTATA 
     3601 AACATGAGCA CGATGATGAT GCAAAACCCA ATTTAGATCC AATCACGCGT ACGCGGCCGC 
     3661 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3721 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3781 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3841 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3901 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3961 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4021 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4081 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4141 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4201 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4261 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4321 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4381 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4441 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4501 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4561 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4621 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4681 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4741 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4801 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4861 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4921 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4981 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5041 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5101 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5161 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5221 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5281 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5341 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5401 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5461 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5521 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5581 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5641 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5701 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5761 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5821 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5881 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5941 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6001 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6061 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6121 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6181 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6241 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6301 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6361 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6421 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6481 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6541 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6601 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6661 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6721 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6781 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6841 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6901 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6961 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7021 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7081 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7141 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7201 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7261 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7321 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7381 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7441 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//