LOCUS       RC205508                7902 bp    DNA     circular  SYN 
DEFINITION  PRPF4B (Myc-DDK-tagged)-Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B)
ACCESSION   NM_003913
VERSION     NM_003913.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4059..4150 
                     /label="Myc-DDK"  
     misc_feature    4180..4765 
                     /label="PolyA signal"  
     misc_feature    4237..4256 
                     /label="Restriction Enzyme"  
     misc_feature    5041..5713 
                     /label="ColE1"  
     misc_feature    6143..6937 
                     /label="Kan/Neo"  
     misc_feature    6972..7274 
                     /label="SV40 ori"  
     misc_feature    7360..7837 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4041..4047 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4041 
                     /label="PRPF4B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGCCGCG GAGACCCAGT CGCTACGGGA GCAGCCAGAG ATGGAAGATG 
     1081 CTAATTCTGA AAAGAGTATA AATGAAGAAA ATGGAGAAGT ATCAGAAGAC CAGTCTCAAA 
     1141 ATAAGCACAG TCGTCACAAA AAAAAGAAGC ATAAACACAG AAGTAAACAT AAGAAACATA 
     1201 AACATTCCTC AGAAGAAGAC AAGGATAAAA AACATAAACA TAAGCATAAA CATAAGAAAC 
     1261 ACAAAAGAAA AGAGGTTATT GATGCTTCTG ATAAAGAGGG TATGTCTCCA GCAAAAAGAA 
     1321 CTAAACTTGA TGATTTAGCT TTGCTAGAAG ACTTGGAAAA ACAGAGAGCC TTGATTAAGG 
     1381 CCGAACTTGA TAATGAGTTA ATGGAAGGAA AGGTCCAGTC TGGTATGGGG CTCATTTTGC 
     1441 AAGGTTATGA GTCTGGCTCT GAAGAAGAGG GGGAAATTCA TGAAAAGGCA AGAAATGGAA 
     1501 ATAGGTCTAG TACTAGATCT TCAAGTACAA AGGGGAAACT TGAACTTGTG GACAATAAAA 
     1561 TTACTACAAA GAAACGAAGT AAAAGCAGAT CCAAAGAACG GACTAGACAT AGGTCTGATA 
     1621 AAAAGAAAAG TAAGGGGGGT ATTGAAATCG TTAAAGAGAA AACAACTAGG AGCAAGTCAA 
     1681 AGGAGAGGAA AAAATCTAAA AGCCCATCCA AAAGAAGTAA GTCTCAAGAT CAAGCAAGGA 
     1741 AATCAAAATC CCCTACCCTT AGAAGGCGAT CTCAAGAGAA AATTGGTAAG GCCAGATCTC 
     1801 CTACTGATGA TAAGGTTAAA ATTGAAGATA AAAGTAAATC AAAAGATAGG AAAAAATCCC 
     1861 CAATTATAAA TGAAAGTAGA AGTCGCGATC GAGGTAAAAA ATCCAGATCC CCAGTTGATT 
     1921 TAAGAGGTAA ATCCAAAGAC AGAAGGTCAC GGTCCAAAGA GAGAAAATCA AAACGGTCTG 
     1981 AAACTGATAA AGAAAAGAAG CCAATTAAAT CTCCCTCTAA AGATGCTTCA TCTGGGAAAG 
     2041 AAAATAGGTC ACCCAGCAGA AGACCTGGTC GTAGTCCTAA AAGAAGAAGT TTGTCTCCAA 
     2101 AACCACGTGA TAAATCAAGA AGAAGCAGGT CTCCACTTTT GAATGATAGA AGATCTAAGC 
     2161 AGAGCAAATC CCCCTCGCGG ACACTGTCTC CTGGGAGAAG AGCCAAGAGC CGATCCTTAG 
     2221 AAAGAAAACG ACGAGAACCA GAGAGGAGAC GACTTTCTTC TCCAAGAACA CGACCTCGAG 
     2281 ATGATATCCT CAGTAGACGT GAAAGATCAA AAGATGCCAG CCCCATCAAT AGATGGTCTC 
     2341 CAACCCGAAG AAGAAGTAGA TCTCCCATTA GAAGGAGGTC TCGTTCCCCA CTCAGACGTA 
     2401 GCAGGTCTCC AAGAAGAAGA AGCAGATCTA CTCGGAGAAG GGACAGAGGT CGGAGGAGCA 
     2461 GATCACGCTT GCGAAGGCGG TCTCGATCAC GCGGTGGTCG TAGACGAAGG AGCAGAAGCA 
     2521 AAGTAAAGGA AGATAAATTT AAAGGAAGTC TTTCTGAAGG AATGAAAGTT GAGCAGGAAT 
     2581 CTTCGTCTGA TGATAACCTT GAAGACTTTG ATGTAGAGGA AGAAGATGAA GAAGCCCTAA 
     2641 TAGAACAGAG AAGAATCCAA AGGCAGGCAA TTGTTCAGAA ATATAAATAC CTTGCTGAAG 
     2701 ATAGCAACAT GTCTGTGCCA TCTGAACCAA GCAGCCCCCA GAGCAGTACG AGAACACGAT 
     2761 CACCATCTCC AGATGACATT CTGGAGCGAG TAGCTGCTGA TGTTAAAGAG TATGAACGGG 
     2821 AAAATGTTGA TACATTTGAG GCCTCAGTGA AAGCCAAGCA TAATCTAATG ACAGTTGAAC 
     2881 AGAATAATGG TTCATCTCAG AAGAAGTTGT TGGCACCTGA TATGTTTACA GAATCTGATG 
     2941 ATATGTTTGC TGCGTATTTT GATAGTGCTC GTCTTCGGGC CGCTGGCATT GGAAAAGATT 
     3001 TCAAAGAGAA TCCCAACCTC AGAGATAACT GGACCGATGC AGAAGGCTAT TATCGTGTGA 
     3061 ACATAGGTGA AGTCCTAGAT AAACGTTACA ATGTGTATGG CTACACTGGG CAAGGTGTAT 
     3121 TCAGTAATGT TGTACGAGCC AGAGATAATG CAAGAGCCAA CCAAGAAGTG GCTGTAAAGA 
     3181 TCATCAGAAA CAATGAGCTC ATGCAAAAGA CTGGTTTAAA AGAATTAGAG TTCTTGAAAA 
     3241 AACTTAATGA TGCTGATCCT GATGACAAAT TTCATTGTCT GAGACTCTTA AGGCACTTCT 
     3301 ATCACAAGCA GCATCTTTGT CTGGTATTCG AGCCTCTCAG CATGAACTTA CGAGAGGTGT 
     3361 TAAAAAAATA TGGTAAAGAT GTTGGTCTTC ATATTAAAGC TGTAAGATCC TATAGTCAGC 
     3421 AGTTGTTCCT GGCATTGAAA CTCCTTAAAA GATGCAATAT CCTACATGCA GATATCAAGC 
     3481 CAGACAATAT CCTGGTTAAT GAATCCAAAA CTATTTTAAA GCTTTGCGAT TTTGGGTCGG 
     3541 CTTCACATGT TGCGGATAAT GACATAACAC CTTATCTTTT CAGTAGATTT TATCGTGCTC 
     3601 CTGAAATCAT TATAGGTAAA AGCTATGACT ATGGTATAGA TATGTGGTCT GTAGGTTGCA 
     3661 CCTTATACGA ACTCTATACT GGAAAAATTT TATTCCCTGG CAAAACCAAT AACCATATGC 
     3721 TGAAGCTTGC AATGGATCTC AAAGGAAAGA TGCCAAATAA GATGATTCGA AAAGGTGTGT 
     3781 TCAAAGATCA GCATTTTGAT CAAAATCTCA ACTTCATGTA CATAGAAGTT GATAAAGTAA 
     3841 CAGAGAGGGA GAAAGTTACT GTTATGAGCA CCATTAATCC AACTAAGGAC CTGTTGGCTG 
     3901 ACTTGATTGG GTGCCAGAGA CTTCCTGAAG ACCAACGTAA GAAAGTACAC CAGCTAAAGG 
     3961 ACTTGTTGGA CCAGATTCTG ATGTTGGACC CAGCTAAACG AATTAGCATC AACCAGGCCC 
     4021 TACAGCACGC CTTCATCCAG GAAAAAATTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4081 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4141 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4201 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4261 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4321 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4381 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4441 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4501 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4561 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4621 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4681 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4741 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4801 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4861 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4921 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4981 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5041 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5101 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5161 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5221 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5281 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5341 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5401 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5461 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5521 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5581 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5641 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5701 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5761 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5821 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5881 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5941 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6001 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6061 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6121 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6181 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6241 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6301 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6361 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6421 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6481 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6541 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6601 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6661 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6721 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6781 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6841 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6901 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6961 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7021 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7081 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7141 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7201 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7261 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7321 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7381 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7441 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7501 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7561 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7621 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7681 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7741 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7801 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7861 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//