LOCUS       RC205447                6394 bp    DNA     circular  SYN 
DEFINITION  ATP6V1B2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2)
ACCESSION   NM_001693
VERSION     NM_001693.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2551..2642 
                     /label="Myc-DDK"  
     misc_feature    2672..3257 
                     /label="PolyA signal"  
     misc_feature    2729..2748 
                     /label="Restriction Enzyme"  
     misc_feature    3533..4205 
                     /label="ColE1"  
     misc_feature    4635..5429 
                     /label="Kan/Neo"  
     misc_feature    5464..5766 
                     /label="SV40 ori"  
     misc_feature    5852..6329 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2533..2539 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2533 
                     /label="ATP6V1B2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTGCGG GCGATGCGGG GGATTGTCAA CGGGGCCGCA CCCGAGCTAC 
     1081 CCGTGCCCAC CGGTGGGCCG GCGGTGGGAG CTCAGGAGCA GGCGCTGGCA GTCAGTCGGA 
     1141 ACTACCTCTC CCAGCCTCGC CTCACATACA AGACAGTATC TGGAGTCAAT GGTCCACTAG 
     1201 TGATCTTAGA TCATGTTAAG TTTCCCAGGT ATGCTGAAAT TGTCCATTTG ACCTTACCGG 
     1261 ATGGCACAAA GAGAAGTGGG CAAGTTCTGG AAGTTAGTGG TTCCAAGGCA GTAGTTCAGG 
     1321 TATTTGAAGG GACTTCAGGT ATAGATGCTA AGAAAACGTC CTGTGAGTTT ACTGGGGATA 
     1381 TTCTCCGAAC ACCGGTGTCT GAGGATATGC TTGGTCGGGT ATTCAATGGA TCGGGAAAAC 
     1441 CCATTGACAG AGGTCCTGTT GTACTGGCCG AAGACTTCCT TGATATCATG GGTCAGCCAA 
     1501 TCAACCCTCA ATGTCGAATC TACCCAGAGG AAATGATTCG GACTGGCATT TCGGCCATCG 
     1561 ATGGGATGAA CAGTATTGCT AGGGGGCAGA AAATTCCTAT CTTCTCTGCT GCTGGGCTAC 
     1621 CACACAATGA GATTGCAGCT CAGATCTGTC GCCAGGCTGG TTTGGTAAAG AAATCCAAAG 
     1681 ATGTAGTAGA CTACAGTGAG GAAAATTTTG CAATTGTATT TGCTGCTATG GGTGTAAACA 
     1741 TGGAAACTGC CCGGTTCTTC AAATCTGACT TTGAAGAAAA TGGCTCAATG GACAATGTCT 
     1801 GCCTCTTTTT GAACTTGGCT AATGACCCAA CCATTGAGCG AATTATCACT CCTCGCCTGG 
     1861 CTCTAACCAC AGCTGAATTT CTGGCGTACC AATGTGAGAA ACATGTATTG GTTATTCTAA 
     1921 CAGACATGAG TTCTTATGCT GAAGCACTTC GAGAGGTTTC AGCAGCCAGG GAAGAGGTAC 
     1981 CTGGTCGACG AGGTTTTCCA GGTTACATGT ATACAGATTT AGCCACGATA TATGAACGCG 
     2041 CTGGGCGAGT GGGAGGGAGA AACGGCTCGA TTACTCAAAT CCCTATTCTA ACCATGCCTA 
     2101 ATGATGATAT CACTCACCCC ATCCCAGACT TGACTGGCTA CATTACAGAG GGGCAGATCT 
     2161 ATGTGGACAG ACAGCTGCAC AACAGACAGA TTTATCCACC TATCAATGTG CTGCCCTCAC 
     2221 TATCACGGTT AATGAAGTCT GCTATTGGAG AAGGGATGAC CAGGAAGGAT CATGCCGATG 
     2281 TATCTAACCA GCTATATGCG TGCTATGCTA TTGGAAAGGA TGTGCAAGCC ATGAAAGCTG 
     2341 TCGTTGGAGA AGAAGCCCTT ACCTCAGATG ATCTTCTCTA CTTGGAATTT CTGCAGAAGT 
     2401 TTGAGAGGAA CTTCATTGCT CAGGGTCCTT ACGAAAATCG CACTGTCTTT GAGACTTTGG 
     2461 ACATTGGCTG GCAGCTACTC CGAATCTTCC CCAAAGAAAT GCTGAAGAGA ATCCCTCAGA 
     2521 GCACCCTCAG CGAATTTTAC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA 
     2581 GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG 
     2641 GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC 
     2701 CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT 
     2761 AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG 
     2821 GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA 
     2881 TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG 
     2941 GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC 
     3001 CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT 
     3061 GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT 
     3121 ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA 
     3181 GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG 
     3241 TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT 
     3301 GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     3361 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     3421 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     3481 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     3541 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     3601 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     3661 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     3721 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     3781 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     3841 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     3901 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     3961 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 
     4021 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 
     4081 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 
     4141 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG 
     4201 CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG 
     4261 GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG 
     4321 GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA 
     4381 CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG 
     4441 TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT 
     4501 CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT 
     4561 CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG 
     4621 TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT 
     4681 GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC 
     4741 GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC 
     4801 ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG 
     4861 CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG 
     4921 CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC 
     4981 GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC 
     5041 GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA 
     5101 TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA 
     5161 TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC 
     5221 CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA 
     5281 CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC 
     5341 ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC 
     5401 GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT 
     5461 CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA 
     5521 ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT 
     5581 GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG 
     5641 GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC 
     5701 CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC 
     5761 CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT 
     5821 TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 
     5881 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 
     5941 ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT 
     6001 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC 
     6061 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG 
     6121 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA 
     6181 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT 
     6241 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT 
     6301 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA 
     6361 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//