LOCUS       RC205380                8298 bp    DNA     circular  SYN 
DEFINITION  RBL2 (Myc-DDK-tagged)-Human retinoblastoma-like 2 (p130) (RBL2)
ACCESSION   NM_005611
VERSION     NM_005611.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4455..4546 
                     /label="Myc-DDK"  
     misc_feature    4576..5161 
                     /label="PolyA signal"  
     misc_feature    4633..4652 
                     /label="Restriction Enzyme"  
     misc_feature    5437..6109 
                     /label="ColE1"  
     misc_feature    6539..7333 
                     /label="Kan/Neo"  
     misc_feature    7368..7670 
                     /label="SV40 ori"  
     misc_feature    7756..8233 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4437..4443 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4437 
                     /label="RBL2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGTCGGGA GGTGACCAGT CGCCACCGCC CCCGCCTCCC CCTCCGGCGG 
     1081 CGGCAGCCTC GGATGAGGAG GAGGAGGACG ACGGCGAGGC GGAAGACGCC GCGCCGCCTG 
     1141 CCGAGTCGCC CACCCCTCAG ATCCAGCAGC GGTTCGACGA GCTGTGCAGC CGCCTCAACA 
     1201 TGGACGAGGC GGCGCGGGCC GAGGCCTGGG ACAGCTACCG CAGCATGAGC GAAAGCTACA 
     1261 CGCTGGAGGG AAATGATCTT CATTGGTTAG CATGTGCCTT ATATGTGGCT TGCAGAAAAT 
     1321 CTGTTCCAAC TGTAAGCAAA GGGACAGTGG AAGGAAACTA TGTATCTTTA ACTAGAATCC 
     1381 TGAAATGTTC AGAGCAGAGC TTAATCGAAT TTTTTAATAA GATGAAGAAG TGGGAAGACA 
     1441 TGGCAAATCT ACCCCCACAT TTCAGAGAAC GTACTGAGAG ATTAGAAAGA AACTTCACTG 
     1501 TTTCTGCTGT AATTTTTAAG AAATATGAAC CCATTTTTCA GGACATCTTT AAATACCCTC 
     1561 AAGAGGAGCA ACCTCGTCAG CAGCGAGGAA GGAAACAGCG GCGACAGCCC TGTACTGTGT 
     1621 CTGAAATTTT CCATTTTTGT TGGGTGCTTT TTATATATGC AAAAGGTAAT TTCCCCATGA 
     1681 TTAGTGATGA TTTGGTCAAT TCTTATCACC TGCTGCTGTG TGCTTTGGAC TTAGTTTATG 
     1741 GAAATGCACT TCAGTGTTCT AATCGTAAAG AACTTGTGAA CCCTAATTTT AAAGGCTTAT 
     1801 CTGAAGATTT TCATGCTAAA GATTCTAAAC CTTCCTCTGA CCCCCCTTGT ATCATTGAGA 
     1861 AACTGTGTTC CTTACATGAT GGCCTAGTTT TGGAAGCAAA GGGGATAAAG GAACATTTCT 
     1921 GGAAACCCTA TATTAGGAAA CTTTATGAAA AAAAGCTCCT TAAGGGAAAA GAAGAAAATC 
     1981 TCACTGGGTT TCTAGAACCT GGGAACTTTG GAGAGAGTTT TAAAGCCATC AATAAGGCCT 
     2041 ATGAGGAGTA TGTTTTATCT GTTGGGAATT TAGATGAGCG GATATTTCTT GGAGAGGATG 
     2101 CTGAGGAGGA AATTGGGACT CTCTCAAGGT GTCTGAACGC TGGTTCAGGA ACAGAGACTG 
     2161 CTGAAAGGGT GCAGATGAAA AACATCTTAC AGCAGCATTT TGACAAGTCC AAAGCACTTA 
     2221 GAATCTCCAC ACCACTAACT GGTGTTAGGT ACATTAAGGA GAATAGCCCT TGTGTGACTC 
     2281 CAGTTTCTAC AGCTACGCAT AGCTTGAGTC GTCTTCACAC CATGCTGACA GGCCTCAGGA 
     2341 ATGCACCAAG TGAGAAACTG GAACAGATTC TCAGGACATG TTCCAGAGAT CCAACCCAGG 
     2401 CTATTGCTAA CAGACTGAAA GAAATGTTTG AAATATATTC TCAGCATTTC CAGCCAGACG 
     2461 AGGATTTCAG TAATTGTGCT AAAGAAATTG CCAGCAAACA TTTTCGTTTT GCGGAGATGC 
     2521 TTTACTATAA AGTATTAGAA TCTGTTATTG AGCAGGAACA AAAAAGACTA GGAGACATGG 
     2581 ATTTATCTGG TATTCTGGAA CAAGATGCGT TCCACAGATC TCTCTTGGCC TGCTGCCTTG 
     2641 AGGTCGTCAC TTTTTCTTAT AAGCCTCCTG GGAATTTTCC ATTTATTACT GAAATATTTG 
     2701 ATGTGCCTCT TTATCATTTT TATAAGGTGA TAGAAGTATT CATTAGAGCA GAAGATGGCC 
     2761 TTTGTAGAGA GGTGGTAAAA CACCTTAATC AGATTGAAGA ACAGATCTTA GATCATTTGG 
     2821 CATGGAAACC AGAGTCTCCA CTCTGGGAAA AAATTAGAGA CAATGAAAAC AGAGTTCCTA 
     2881 CATGTGAAGA GGTCATGCCA CCTCAGAACC TGGAAAGGGC AGATGAAATT TGCATTGCTG 
     2941 GCTCCCCTTT GACTCCCAGA AGGGTGACTG AAGTTCGTGC TGATACTGGA GGACTTGGAA 
     3001 GGAGCATAAC ATCTCCAACC ACATTATACG ATAGGTACAG CTCCCCACCA GCCAGCACTA 
     3061 CCAGAAGGCG GCTATTTGTT GAGAATGATA GCCCCTCTGA TGGAGGGACG CCTGGGCGCA 
     3121 TGCCCCCACA GCCCCTAGTC AATGCTGTCC CTGTGCAGAA TGTATCTGGG GAGACTGTTT 
     3181 CTGTCACACC AGTTCCTGGA CAGACTTTGG TCACCATGGC AACCGCCACT GTCACAGCCA 
     3241 ACAATGGGCA AACGGTAACC ATTCCTGTGC AAGGTATTGC CAATGAAAAT GGAGGGATAA 
     3301 CATTCTTCCC TGTCCAAGTC AATGTTGGGG GGCAGGCACA AGCTGTGACA GGCTCCATCC 
     3361 AGCCCCTCAG TGCTCAGGCC CTGGCTGGAA GTCTGAGCTC TCAACAGGTG ACAGGAACAA 
     3421 CTTTGCAAGT CCCTGGTCAA GTGGCCATTC AACAGATTTC CCCAGGTGGC CAACAGCAGA 
     3481 AGCAAGGCCA GTCTGTAACC AGCAGTAGTA ATAGACCCAG GAAGACCAGC TCTTTATCGC 
     3541 TTTTCTTTAG AAAGGTATAC CATTTAGCAG CTGTCCGCCT TCGGGATCTC TGTGCCAAAC 
     3601 TAGATATTTC AGATGAATTG AGGAAAAAAA TCTGGACCTG CTTTGAATTC TCCATAATTC 
     3661 AGTGTCCTGA ACTTATGATG GACAGACATC TGGACCAGTT ATTAATGTGT GCCATTTATG 
     3721 TGATGGCAAA GGTCACAAAA GAAGATAAGT CCTTCCAGAA CATTATGCGT TGTTATAGGA 
     3781 CTCAGCCGCA GGCCCGGAGC CAGGTGTATA GAAGTGTTTT GATAAAAGGA AAAAGAAAAA 
     3841 GAAGAAATTC TGGCAGCAGT GATAGCAGAA GCCATCAGAA TTCTCCAACA GAACTAAACA 
     3901 AAGATAGAAC CAGTAGAGAC TCCAGTCCAG TTATGAGGTC AAGCAGCACC TTGCCAGTTC 
     3961 CACAGCCCAG CAGTGCTCCT CCCACACCTA CTCGCCTCAC AGGTGCCAAC AGTGACATGG 
     4021 AAGAAGAGGA GAGGGGAGAC CTCATTCAGT TCTACAACAA CATCTACATC AAACAGATTA 
     4081 AGACATTTGC CATGAAGTAC TCACAGGCAA ATATGGATGC TCCTCCACTC TCTCCCTATC 
     4141 CATTTGTAAG AACAGGCTCC CCTCGCCGAA TACAGTTGTC TCAAAATCAT CCTGTCTACA 
     4201 TTTCCCCACA TAAAAATGAA ACAATGCTTT CTCCTCGAGA AAAGATTTTC TATTACTTCA 
     4261 GCAACAGTCC TTCAAAGAGA CTGAGAGAAA TTAATAGTAT GATACACACA GGAGAAACTC 
     4321 CTACTAAAAA GAGAGGAATT CTTTTGGAAG ATGGAAGTGA ATCACCTGCA AAAAGAATTT 
     4381 GCCCAGAAAA TCATTCTGCC TTATTACGCC GTCTCCAAGA TGTAGCTAAT GACCGTGGTT 
     4441 CCCACACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4501 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4561 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4621 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4681 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4741 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4801 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4861 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4921 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4981 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5041 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5101 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5161 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5221 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5281 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5341 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5401 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5461 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5521 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5581 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5641 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5701 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5761 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5821 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5881 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5941 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6001 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6061 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6121 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6181 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6241 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6301 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6361 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6421 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6481 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6541 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6601 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6661 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6721 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6781 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6841 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6901 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6961 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7021 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7081 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7141 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7201 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7261 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7321 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7381 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7441 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7501 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7561 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7621 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7681 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7741 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7801 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7861 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7921 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7981 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8041 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8101 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8161 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8221 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8281 AAATTTAACG CGAATTTT
//