LOCUS       RC205326                6930 bp    DNA     circular  SYN 
DEFINITION  FBLN1 (Myc-DDK-tagged)-Human fibulin 1 (FBLN1), transcript variant C
ACCESSION   NM_001996
VERSION     NM_001996.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3087..3178 
                     /label="Myc-DDK"  
     misc_feature    3208..3793 
                     /label="PolyA signal"  
     misc_feature    3265..3284 
                     /label="Restriction Enzyme"  
     misc_feature    4069..4741 
                     /label="ColE1"  
     misc_feature    5171..5965 
                     /label="Kan/Neo"  
     misc_feature    6000..6302 
                     /label="SV40 ori"  
     misc_feature    6388..6865 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3069..3075 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3069 
                     /label="FBLN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGCGCC GCGCCGTCGC GCCGGGTCCC GCTTCCGCTG CTGCTGCTCG 
     1081 GCGGCCTTGC GCTGCTGGCG GCCGGAGTGG ACGCGGATGT CCTCCTGGAG GCCTGCTGTG 
     1141 CGGACGGACA CCGGATGGCC ACTCATCAGA AGGACTGCTC GCTGCCATAT GCTACGGAAT 
     1201 CCAAAGAATG CAGGATGGTG CAGGAGCAGT GCTGCCACAG CCAGCTGGAG GAGCTGCACT 
     1261 GTGCCACGGG CATCAGCCTG GCCAACGAGC AGGACCGCTG TGCCACGCCC CACGGTGACA 
     1321 ACGCCAGCCT GGAGGCCACA TTTGTGAAGA GGTGCTGCCA TTGCTGTCTG CTGGGGAGGG 
     1381 CGGCCCAGGC CCAGGGCCAG AGCTGCGAGT ACAGCCTCAT GGTTGGCTAC CAGTGTGGAC 
     1441 AGGTCTTCCG GGCATGCTGT GTCAAGAGCC AGGAGACCGG AGATTTGGAT GTCGGGGGCC 
     1501 TCCAAGAAAC GGATAAGATC ATTGAGGTTG AGGAGGAACA AGAGGACCCA TATCTGAATG 
     1561 ACCGCTGCCG AGGAGGCGGG CCCTGCAAGC AGCAGTGCCG AGACACGGGT GACGAGGTGG 
     1621 TCTGCTCCTG CTTCGTGGGC TACCAGCTGC TGTCTGATGG TGTCTCCTGT GAAGATGTCA 
     1681 ATGAATGCAT CACGGGCAGC CACAGCTGCC GGCTTGGAGA ATCCTGCATC AACACAGTGG 
     1741 GCTCTTTCCG CTGCCAGCGG GACAGCAGCT GCGGGACTGG CTATGAGCTC ACAGAGGACA 
     1801 ATAGCTGCAA AGATATTGAC GAGTGTGAGA GTGGTATTCA TAACTGCCTC CCCGATTTTA 
     1861 TCTGTCAGAA TACTCTGGGA TCCTTCCGCT GCCGACCCAA GCTACAGTGC AAGAGTGGCT 
     1921 TTATACAAGA TGCTCTAGGC AACTGTATTG ATATCAATGA GTGTTTGAGT ATCAGTGCCC 
     1981 CGTGCCCTAT CGGGCATACA TGCATCAACA CAGAGGGCTC CTACACGTGC CAGAAGAACG 
     2041 TGCCCAACTG TGGCCGTGGC TACCATCTCA ACGAGGAGGG AACGCGCTGT GTTGATGTGG 
     2101 ACGAGTGCGC GCCACCTGCT GAGCCCTGTG GGAAGGGACA TCGCTGCGTG AACTCTCCCG 
     2161 GCAGTTTCCG CTGCGAATGC AAGACGGGTT ACTATTTTGA CGGCATCAGC AGGATGTGTG 
     2221 TCGATGTCAA CGAGTGCCAG CGCTACCCCG GGCGCCTGTG TGGCCACAAG TGCGAGAACA 
     2281 CGCTGGGCTC CTACCTCTGC AGCTGTTCCG TGGGCTTCCG GCTCTCTGTG GATGGCAGGT 
     2341 CATGTGAAGA CATCAATGAG TGCAGCAGCA GCCCCTGTAG CCAGGAGTGT GCCAACGTCT 
     2401 ACGGCTCCTA CCAGTGTTAC TGCCGGCGAG GCTACCAGCT CAGCGATGTG GATGGAGTCA 
     2461 CCTGTGAAGA CATCGACGAG TGCGCCCTGC CCACCGGGGG CCACATCTGC TCCTACCGCT 
     2521 GCATCAACAT CCCTGGAAGC TTCCAGTGCA GCTGCCCCTC GTCTGGCTAC AGGCTGGCCC 
     2581 CCAATGGCCG CAACTGCCAA GACATTGATG AGTGTGTGAC TGGCATCCAC AACTGCTCCA 
     2641 TCAACGAGAC CTGCTTCAAC ATCCAGGGCG GCTTCCGCTG CCTGGCCTTC GAGTGCCCTG 
     2701 AGAACTACCG CCGCTCCGCA GCCACCCGCT GTGAGCGCTT GCCTTGCCAT GAGAATCGGG 
     2761 AGTGCTCCAA GCTGCCTCTG AGAATAACCT ACTACCACCT CTCTTTCCCC ACCAACATCC 
     2821 AAGCGCCCGC GGTGGTTTTC CGCATGGGCC CCTCCAGTGC TGTCCCCGGG GACAGCATGC 
     2881 AGCTGGCCAT CACCGGCGGC AATGAGGAGG GCTTTTTCAC CACCCGGAAG GTGAGCCCCC 
     2941 ACAGTGGGGT GGTGGCCCTC ACCAAGCCTG TCCCCGAGCC CAGGGACTTG CTCCTGACCG 
     3001 TCAAGATGGA TCTCTCTCGC CACGGCACCG TCAGCTCCTT TGTGGCCAAG CTTTTCATCT 
     3061 TTGTGTCTGC AGAGCTCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3121 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3181 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3241 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3301 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3361 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     3421 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     3481 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     3541 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     3601 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     3661 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     3721 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     3781 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     3841 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     3901 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     3961 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4021 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4081 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4141 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4201 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4261 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4321 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4381 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     4441 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     4501 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     4561 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     4621 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     4681 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     4741 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     4801 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     4861 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     4921 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     4981 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5041 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5101 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5161 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5221 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5281 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5341 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     5401 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     5461 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     5521 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     5581 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     5641 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     5701 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     5761 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     5821 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     5881 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     5941 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6001 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6061 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6121 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6181 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6241 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6301 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6361 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     6421 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     6481 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     6541 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     6601 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     6661 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     6721 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     6781 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     6841 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     6901 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//