LOCUS       RC205325                6654 bp    DNA     circular  SYN 
DEFINITION  FZD9 (Myc-DDK-tagged)-Human frizzled family receptor 9 (FZD9)
ACCESSION   NM_003508
VERSION     NM_003508.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2811..2902 
                     /label="Myc-DDK"  
     misc_feature    2932..3517 
                     /label="PolyA signal"  
     misc_feature    2989..3008 
                     /label="Restriction Enzyme"  
     misc_feature    3793..4465 
                     /label="ColE1"  
     misc_feature    4895..5689 
                     /label="Kan/Neo"  
     misc_feature    5724..6026 
                     /label="SV40 ori"  
     misc_feature    6112..6589 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2793..2799 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2793 
                     /label="FZD9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGTGGCG CCTCTGCGGG GGGCGCTGCT GCTGTGGCAG CTGCTGGCGG 
     1081 CGGGCGGCGC GGCACTGGAG ATCGGCCGCT TCGACCCGGA GCGCGGGCGC GGGGCTGCGC 
     1141 CGTGCCAGGC GGTGGAGATC CCCATGTGCC GCGGCATCGG CTACAACCTG ACCCGCATGC 
     1201 CCAACCTGCT GGGCCACACG TCGCAGGGCG AGGCGGCTGC CGAGCTAGCG GAGTTCGCGC 
     1261 CGCTGGTGCA GTACGGCTGC CACAGCCACC TGCGCTTCTT CCTGTGCTCG CTCTACGCGC 
     1321 CCATGTGCAC CGACCAGGTC TCGACGCCCA TTCCCGCCTG CCGGCCCATG TGCGAGCAGG 
     1381 CGCGCCTGCG CTGCGCGCCC ATCATGGAGC AGTTCAACTT CGGCTGGCCG GACTCGCTCG 
     1441 ACTGCGCCCG GCTGCCCACG CGCAACGACC CGCACGCGCT GTGCATGGAG GCGCCCGAGA 
     1501 ACGCCACGGC CGGCCCCGCG GAGCCCCACA AGGGCCTGGG CATGCTGCCC GTGGCGCCGC 
     1561 GGCCCGCGCG CCCTCCCGGA GACCTGGGCC CGGGCGCGGG CGGCAGTGGC ACCTGCGAGA 
     1621 ACCGCGAGAA GTTCCAGTAC GTGGAGAAGA GCCGCTCGTG CGCACCGCGC TGCGGGCCCG 
     1681 GCGTCGAGGT GTTCTGGTCC CGGCGCGACA AGGACTTCGC GCTGGTCTGG ATGGCCGTGT 
     1741 GGTCGGCGCT GTGCTTCTTC TCCACCGCCT TCACTGTGCT CACCTTCTTG CTGGAGCCCC 
     1801 ACCGCTTCCA GTACCCCGAG CGCCCCATCA TCTTCCTCTC CATGTGCTAC AACGTCTACT 
     1861 CGCTGGCCTT CCTGATCCGT GCGGTGGCCG GAGCGCAGAG CGTGGCCTGT GACCAGGAGG 
     1921 CGGGCGCGCT CTACGTGATC CAGGAGGGCC TGGAGAACAC GGGCTGCACG CTGGTCTTCC 
     1981 TACTGCTCTA CTACTTCGGC ATGGCCAGCT CGCTCTGGTG GGTGGTCCTG ACGCTCACCT 
     2041 GGTTCCTGGC TGCCGGGAAG AAATGGGGCC ACGAGGCCAT CGAGGCCCAC GGCAGCTATT 
     2101 TCCACATGGC TGCCTGGGGC CTGCCCGCGC TCAAGACCAT CGTCATCCTG ACCCTGCGCA 
     2161 AGGTGGCGGG TGATGAGCTG ACTGGGCTTT GCTACGTGGC CAGCACGGAT GCAGCAGCGC 
     2221 TCACGGGCTT CGTGCTGGTG CCCCTCTCTG GCTACCTGGT GCTGGGCAGT AGTTTCCTCC 
     2281 TGACCGGCTT CGTGGCCCTC TTCCACATCC GCAAGATCAT GAAGACGGGC GGCACCAACA 
     2341 CAGAGAAGCT GGAGAAGCTC ATGGTCAAGG TCGGGGTCTT CTCCATCCTC TACACGGTGC 
     2401 CCGCCACCTG CGTCATCGTT TGCTATGTCT ACGAACGCCT CAACATGGAC TTCTGGCGCC 
     2461 TTCGGGCCAC AGAGCAGCCA TGCGCAGCGG CCGCGGGGCC CGGAGGCCGG AGGGACTGCT 
     2521 CGCTGCCAGG GGGCTCGGTG CCCACCGTGG CGGTCTTCAT GCTCAAAATT TTCATGTCAC 
     2581 TGGTGGTGGG GATCACCAAC GGCGTCTGGG TGTGGAGCTC CAAGACTTTC CAGACCTGGC 
     2641 AGAGCCTGTG CTACCGCAAG ATAGCAGCTG GCCGGGCCCG GGCCAAGGCC TGCCACGCCC 
     2701 CCGGGAGCTA CGGACGTGGC ACGCACTGCC ACTATAAGGC TCCCACCGTG GTCTTGCACA 
     2761 TGACTAAGAC GGACCCCTCT TTGGAGAACC CCACACACCT CACGCGTACG CGGCCGCTCG 
     2821 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     2881 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     2941 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3001 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3061 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3121 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3181 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3241 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3301 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     3361 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     3421 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     3481 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     3541 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     3601 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     3661 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     3721 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     3781 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     3841 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     3901 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     3961 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4021 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4081 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4141 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4201 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4261 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4321 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     4381 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     4441 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     4501 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     4561 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     4621 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     4681 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     4741 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     4801 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     4861 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     4921 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     4981 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5041 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5101 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5161 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5221 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5281 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     5341 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     5401 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     5461 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     5521 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     5581 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     5641 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     5701 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     5761 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     5821 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     5881 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     5941 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6001 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6061 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6121 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6181 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6241 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6301 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     6361 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     6421 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     6481 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     6541 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     6601 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//