LOCUS       RC205206                8478 bp    DNA     circular  SYN 
DEFINITION  TTLL4 (Myc-DDK-tagged)-Human tubulin tyrosine ligase-like family, member 4 (TTLL4)
ACCESSION   NM_014640
VERSION     NM_014640.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4635..4726 
                     /label="Myc-DDK"  
     misc_feature    4756..5341 
                     /label="PolyA signal"  
     misc_feature    4813..4832 
                     /label="Restriction Enzyme"  
     misc_feature    5617..6289 
                     /label="ColE1"  
     misc_feature    6719..7513 
                     /label="Kan/Neo"  
     misc_feature    7548..7850 
                     /label="SV40 ori"  
     misc_feature    7936..8413 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4617..4623 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4617 
                     /label="TTLL4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCAGCA GGAACACAGC ACTATAGTAT TGGCCTCCGC CAGAAAAGCA 
     1081 GCTTCAAGCA GAGTGGTCCC TCAGGCACAG TACCTGCCAC GCCACCTGAG AAACCCTCGG 
     1141 AGGGCAGAGT CTGGCCTCAG GCCCATCAGC AAGTGAAGCC AATCTGGAAG CTGGAAAAGA 
     1201 AGCAAGTGGA GACACTGTCA GCAGGGTTGG GCCCAGGCCT CTTGGGCGTC CCACCCCAGC 
     1261 CAGCATATTT CTTTTGCCCC AGCACTTTAT GTAGCTCTGG GACCACGGCT GTCATTGCAG 
     1321 GCCACAGCAG TTCCTGTTAC CTACACTCTC TCCCGGACTT GTTCAACAGC ACCCTGCTAT 
     1381 ACCGCCGCTC CAGCTATAGG CAAAAACCGT ACCAGCAACT GGAGTCTTTC TGCTTGCGTT 
     1441 CGAGCCCGTC AGAAAAAAGC CCTTTTTCTC TCCCTCAAAA GAGCCTCCCT GTCAGTCTCA 
     1501 CTGCCAACAA GGCCACTTCT TCCATGGTCT TCTCCATGGC CCAGCCCATG GCCTCCTCAT 
     1561 CCACAGAACC ATACCTCTGC TTGGCAGCGG CTGGGGAAAA CCCTTCAGGG AAGAGCCTGG 
     1621 CCTCTGCCAT CTCAGGGAAG ATCCCATCTC CACTCTCTTC CTCCTATAAG CCCATGCTGA 
     1681 ATAATAATTC CTTCATGTGG CCAAATAGCA CGCCAGTGCC TTTATTGCAG ACCACACAGG 
     1741 GCCTGAAGCC AGTATCGCCA CCCAAGATCC AGCCTGTCTC CTGGCATCAT TCAGGGGGTA 
     1801 CTGGAGACTG TGCACCGCAG CCTGTTGACC ATAAGGTGCC CAAAAGCATT GGCACTGTCC 
     1861 CAGCTGATGC CAGTGCCCAT ATCGCCTTGT CTACCGCTAG CTCCCACGAC ACATCCACCA 
     1921 CCAGTGTTGC CTCTTCCTGG TATAACCGGA ATAACTTAGC CATGAGGGCA GAGCCACTTT 
     1981 CCTGTGCTCT GGATGACAGC TCTGATTCCC AGGATCCAAC TAAGGAGATT CGGTTCACTG 
     2041 AGGCCGTGAG GAAATTGACC GCAAGAGGCT TTGAGAAGAT GCCGAGGCAA GGCTGCCAGC 
     2101 TTGAACAGTC TAGTTTCCTG AACCCCAGCT TCCAGTGGAA TGTCCTCAAC AGGAGCAGGC 
     2161 GGTGGAAACC TCCTGCGGTA AATCAGCAGT TTCCTCAGGA GGATGCTGGA TCGGTCAGGC 
     2221 GGGTCCTCCC TGGTGCCTCA GATACCTTGG GGTTGGACAA TACAGTCTTC TGTACCAAGC 
     2281 GTATCAGCAT TCACCTCCTT GCCTCACATG CCAGTGGGCT CAATCACAAC CCTGCCTGTG 
     2341 AATCTGTAAT TGACTCCTCA GCATTTGGAG AAGGCAAAGC TCCAGGTCCC CCTTTTCCTC 
     2401 AAACTCTTGG CATAGCCAAC GTGGCCACCC GCCTCTCTTC CATCCAGCTG GGCCAGTCTG 
     2461 AGAAGGAGAG ACCTGAGGAG GCCAGGGAGC TGGACTCATC TGATAGGGAT ATTAGTTCAG 
     2521 CTACTGACCT CCAGCCAGAT CAGGCTGAGA CTGAAGATAC AGAAGAAGAA CTAGTAGATA 
     2581 GTTTGGAAGA CTGTTGTGGC CGTGATGAGA ATGAAGAGGA GGAGGGAGAC TCAGAGTGCT 
     2641 CCTCATTAAG TGCTGTCTCC CCCAGCGAAT CGGTGGCCAT GATCTCTAGA AGCTGTATGG 
     2701 AAATTCTGAC CAAACCCCTT TCCAATCATG AGAAAGTTGT CCGACCAGCC CTCATCTACA 
     2761 GTCTCTTTCC CAACGTTCCC CCTACCATCT ATTTTGGCAC TCGGGATGAG AGAGTGGAGA 
     2821 AACTTCCCTG GGAACAGAGG AAGTTGCTCC GATGGAAGAT GAGCACAGTG ACCCCCAACA 
     2881 TTGTCAAGCA GACCATTGGA CGGTCCCACT TCAAAATCAG CAAAAGAAAC GATGACTGGC 
     2941 TGGGCTGCTG GGGTCACCAC ATGAAGTCTC CTAGTTTCCG ATCCATTCGA GAGCATCAGA 
     3001 AGCTAAACCA TTTCCCAGGC TCATTCCAGA TTGGGAGGAA GGACCGGCTA TGGCGGAACC 
     3061 TGTCACGTAT GCAGAGCCGC TTTGGCAAGA AGGAGTTCAG TTTCTTCCCC CAGTCCTTTA 
     3121 TCCTGCCCCA GGACGCCAAG CTCCTGCGCA AAGCGTGGGA GAGCAGCAGC CGCCAAAAGT 
     3181 GGATTGTGAA GCCACCAGCA TCAGCTCGAG GCATTGGCAT CCAGGTTATT CACAAGTGGA 
     3241 GTCAGCTCCC CAAGCGAAGG CCCCTCCTGG TACAGAGGTA TCTACACAAA CCCTACCTCA 
     3301 TCAGCGGCAG CAAGTTTGAC CTGCGGATCT ATGTTTATGT CACTTCCTAC GATCCTCTGC 
     3361 GGATTTACCT CTTTTCAGAT GGACTGGTCC GCTTTGCCAG TTGCAAGTAT TCGCCTTCCA 
     3421 TGAAGAGCCT TGGCAATAAG TTCATGCACC TGACCAACTA CAGTGTCAAT AAAAAGAATG 
     3481 CCGAGTACCA GGCCAATGCA GATGAAATGG CTTGCCAGGG CCACAAATGG GCACTGAAGG 
     3541 CTTTGTGGAA CTACCTGAGC CAGAAGGGAG TCAATAGCGA CTCCATCTGG GAGAAGATAA 
     3601 AGGATGTTGT TGTCAAAACT ATCATCTCGT CAGAGCCCTA TGTGACCAGC CTGCTCAAGA 
     3661 TGTATGTGCG ACGGCCCTAT AGCTGCCATG AACTCTTTGG TTTTGACATC ATGCTAGACG 
     3721 AAAACCTCAA GCCCTGGGTC CTGGAAGTCA ACATTTCCCC AAGCCTCCAC TCCAGCTCTC 
     3781 CACTGGATAT CAGCATCAAA GGCCAGATGA TTCGTGACCT TCTGAATCTG GCAGGTTTTG 
     3841 TCCTGCCCAA TGCAGAGGAT ATCATTTCCA GCCCCAGCAG CTGCAGCAGC TCCACCACCA 
     3901 GCCTGCCCAC CTCCCCTGGG GACAAATGTC GAATGGCTCC AGAGCATGTC ACTGCACAGA 
     3961 AGATGAAGAA AGCCTATTAT CTGACCCAGA AAATTCCTGA TCAGGACTTC TATGCATCTG 
     4021 TGCTGGATGT CCTGACACCA GATGATGTTC GGATTCTGGT TGAGATGGAA GATGAGTTTT 
     4081 CTCGCCGTGG TCAGTTTGAA CGAATTTTTC CTTCTCATAT CTCCTCTCGC TATCTCCGCT 
     4141 TTTTTGAGCA GCCACGATAT TTCAACATTC TCACCACCCA ATGGGAACAG AAATACCATG 
     4201 GCAACAAGCT TAAAGGAGTA GATCTGCTCC GGAGTTGGTG CTACAAAGGG TTCCACATGG 
     4261 GAGTTGTCTC TGATTCTGCT CCAGTGTGGT CTCTCCCGAC ATCACTTCTG ACTATCTCAA 
     4321 AGGATGACGT GATACTCAAT GCCTTCAGCA AATCAGAGAC TAGCAAGCTG GGAAAACAAA 
     4381 GCTCCTGTGA GGTTAGCCTA CTACTCTCTG AAGACGGGAC CACGCCCAAA TCCAAGAAGA 
     4441 CTCAAGCTGG CCTCTCCCCT TATCCCCAGA AACCCAGTTC CTCAAAGGAC AGTGAGGACA 
     4501 CCAGCAAAGA GCCCAGCCTT TCTACCCAGA CGTTACCTGT GATCAAGTGC TCTGGGCAGA 
     4561 CTTCAAGACT TTCTGCTTCC TCCACTTTCC AGTCAATCAG TGACTCCCTC CTGGCTGTGA 
     4621 GCCCAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4681 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4741 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4801 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4861 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4921 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4981 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5041 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5101 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5161 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5221 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5281 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5341 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5401 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5461 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5521 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5581 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5641 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5701 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5761 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5821 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5881 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5941 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6001 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6061 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6121 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6181 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6241 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6301 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6361 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6421 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6481 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6541 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6601 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6661 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6721 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6781 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6841 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6901 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6961 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7021 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7081 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7141 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7201 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7261 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7321 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7381 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7441 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7501 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7561 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7621 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7681 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7741 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7801 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7861 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7921 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7981 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8041 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8101 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8161 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8221 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8281 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8341 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8401 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8461 AAATTTAACG CGAATTTT
//