LOCUS       RC204434                7329 bp    DNA     circular  SYN 
DEFINITION  NELL2 (Myc-DDK-tagged)-Human NEL-like 2 (chicken) (NELL2), transcript variant 2
ACCESSION   NM_006159
VERSION     NM_006159.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3486..3577 
                     /label="Myc-DDK"  
     misc_feature    3607..4192 
                     /label="PolyA signal"  
     misc_feature    3664..3683 
                     /label="Restriction Enzyme"  
     misc_feature    4468..5140 
                     /label="ColE1"  
     misc_feature    5570..6364 
                     /label="Kan/Neo"  
     misc_feature    6399..6701 
                     /label="SV40 ori"  
     misc_feature    6787..7264 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3468..3474 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3468 
                     /label="NELL2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTCTCGG GTCTTACTGA GAACATTCTG TTTGATCTTC GGTCTCGGAG 
     1081 CAGTTTGGGG GCTTGGTGTG GACCCTTCCC TACAGATTGA CGTCTTAACA GAGTTAGAAC 
     1141 TTGGGGAGTC CACGACCGGA GTGCGTCAGG TCCCGGGGCT GCATAATGGG ACGAAAGCCT 
     1201 TTCTCTTTCA AGATACTCCC AGAAGCATAA AAGCATCCAC TGCTACAGCT GAACAGTTTT 
     1261 TTCAGAAGCT GAGAAATAAA CATGAATTTA CTATTTTGGT GACCCTAAAA CAGACCCACT 
     1321 TAAATTCAGG AGTTATTCTC TCAATTCACC ACTTGGATCA CAGGTACCTG GAACTGGAAA 
     1381 GTAGTGGCCA TCGGAATGAA GTCAGACTGC ATTACCGCTC AGGCAGTCAC CGCCCTCACA 
     1441 CAGAAGTGTT TCCTTACATT TTGGCTGATG ACAAGTGGCA CAAGCTCTCC TTAGCCATCA 
     1501 GTGCTTCCCA TTTGATTTTA CACATTGACT GCAATAAAAT TTATGAAAGG GTAGTAGAAA 
     1561 AGCCCTCCAC AGACTTGCCT CTAGGCACAA CATTTTGGCT AGGACAGAGA AATAATGCGC 
     1621 ATGGATATTT TAAGGGTATA ATGCAAGATG TCCAATTACT TGTCATGCCC CAGGGATTTA 
     1681 TTGCTCAGTG CCCAGATCTT AATCGCACCT GTCCAACTTG CAATGACTTC CATGGACTTG 
     1741 TGCAGAAAAT CATGGAGCTA CAGGATATTT TAGCCAAAAC ATCAGCCAAG CTGTCTCGAG 
     1801 CTGAACAGCG AATGAATAGA TTGGATCAGT GCTATTGTGA AAGGACTTGC ACCATGAAGG 
     1861 GAACCACCTA CCGAGAATTT GAGTCCTGGA TAGACGGCTG TAAGAACTGC ACATGCCTGA 
     1921 ATGGAACCAT CCAGTGTGAA ACTCTAATCT GCCCAAATCC TGACTGCCCA CTTAAGTCGG 
     1981 CTCTTGCGTA TGTGGATGGC AAATGCTGTA AGGAATGCAA ATCGATATGC CAATTTCAAG 
     2041 GACGAACCTA CTTTGAAGGA GAAAGAAATA CAGTCTATTC CTCTTCTGGA GTATGTGTTC 
     2101 TCTATGAGTG CAAGGACCAG ACCATGAAAC TTGTTGAGAG TTCAGGCTGT CCAGCTTTGG 
     2161 ATTGTCCAGA GTCTCATCAG ATAACCTTGT CTCACAGCTG TTGCAAAGTT TGTAAAGGTT 
     2221 ATGACTTTTG TTCTGAAAGG CATAACTGCA TGGAGAATTC CATCTGCAGA AATCTGAATG 
     2281 ACAGGGCTGT TTGTAGCTGT CGAGATGGTT TTAGGGCTCT TCGAGAGGAT AATGCCTACT 
     2341 GTGAAGACAT CGATGAGTGT GCTGAAGGGC GCCATTACTG TCGTGAAAAT ACAATGTGTG 
     2401 TCAACACCCC GGGTTCTTTT ATGTGCATCT GCAAAACTGG ATACATCAGA ATTGATGATT 
     2461 ATTCATGTAC AGAACATGAT GAGTGTATCA CAAATCAGCA CAACTGTGAT GAAAATGCTT 
     2521 TATGCTTCAA CACTGTTGGA GGACACAACT GTGTTTGCAA GCCGGGCTAT ACAGGGAATG 
     2581 GAACGACATG CAAAGCATTT TGCAAAGATG GCTGTAGGAA TGGAGGAGCC TGTATTGCCG 
     2641 CTAATGTGTG TGCCTGCCCA CAAGGCTTCA CTGGACCCAG CTGTGAAACG GACATTGATG 
     2701 AATGCTCTGA TGGTTTTGTT CAATGTGACA GTCGTGCTAA TTGCATTAAC CTGCCTGGAT 
     2761 GGTACCACTG TGAGTGCAGA GATGGCTACC ATGACAATGG GATGTTTTCA CCAAGTGGAG 
     2821 AATCGTGTGA AGATATTGAT GAGTGTGGGA CCGGGAGGCA CAGCTGTGCC AATGATACCA 
     2881 TTTGCTTCAA TTTGGATGGC GGATATGATT GTCGATGTCC TCATGGAAAG AATTGCACAG 
     2941 GGGACTGCAT CCATGATGGA AAAGTTAAGC ACAATGGTCA GATTTGGGTG TTGGAAAATG 
     3001 ACAGGTGCTC TGTGTGCTCA TGTCAGAATG GATTCGTTAT GTGTCGACGG ATGGTCTGTG 
     3061 ACTGTGAGAA TCCCACAGTT GATCTTTTTT GCTGCCCTGA ATGTGACCCA AGGCTTAGTA 
     3121 GTCAGTGCCT CCATCAAAAT GGGGAAACTT TGTATAACAG TGGTGACACC TGGGTCCAGA 
     3181 ATTGTCAACA GTGCCGCTGC TTGCAAGGGG AAGTTGATTG TTGGCCCCTG CCTTGCCCAG 
     3241 ATGTGGAGTG TGAATTCAGC ATTCTCCCAG AGAATGAGTG CTGCCCGCGC TGTGTCACAG 
     3301 ACCCTTGCCA GGCTGACACC ATCCGCAATG ACATCACCAA GACTTGCCTG GACGAAATGA 
     3361 ATGTGGTTCG CTTCACCGGG TCCTCTTGGA TCAAACATGG CACTGAGTGT ACTCTCTGCC 
     3421 AGTGCAAGAA TGGCCACATC TGTTGCTCAG TGGATCCACA GTGCCTTCAG GAACTGACGC 
     3481 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3541 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3601 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3661 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3721 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3781 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3841 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3901 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3961 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4021 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4081 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4141 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4201 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4261 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4321 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4381 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4441 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4501 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4561 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4621 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4681 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4741 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4801 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4861 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4921 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4981 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5041 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5101 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5161 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5221 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5281 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5341 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5401 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5461 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5521 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5581 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5641 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5701 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5761 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5821 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5881 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5941 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6001 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6061 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6121 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6181 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6241 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6301 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6361 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6421 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6481 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6541 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6601 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6661 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6721 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6781 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6841 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6901 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6961 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7021 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7081 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7141 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7201 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7261 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7321 GCGAATTTT
//