LOCUS       RC204410                8349 bp    DNA     circular  SYN 
DEFINITION  NUP133 (Myc-DDK-tagged)-Human nucleoporin 133kDa (NUP133)
ACCESSION   NM_018230
VERSION     NM_018230.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4506..4597 
                     /label="Myc-DDK"  
     misc_feature    4627..5212 
                     /label="PolyA signal"  
     misc_feature    4684..4703 
                     /label="Restriction Enzyme"  
     misc_feature    5488..6160 
                     /label="ColE1"  
     misc_feature    6590..7384 
                     /label="Kan/Neo"  
     misc_feature    7419..7721 
                     /label="SV40 ori"  
     misc_feature    7807..8284 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4488..4494 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4488 
                     /label="NUP133"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCCCAGCC GCCCCTTCTC CGCGGACCCC GGGTACCGGG TCCCGAAGGG 
     1081 GCCCGCTGGC CGGACTCGGG CCCGGCTCCA CGCCCCGGAC GGCTAGCAGG AAGGGTCTGC 
     1141 CCCTGGGGTC TGCAGTCAGC TCCCCAGTGC TCTTCTCGCC GGTCGGCCGG CGTAGCTCGC 
     1201 TAAGCTCGCG GGGAACACCA ACACGAATGT TCCCACACCA CTCCATAACT GAGTCTGTGA 
     1261 ACTATGATGT GAAAACGTTT GGATCTTCTC TTCCTGTTAA AGTCATGGAA GCCCTAACAT 
     1321 TGGCTGAAGT CGATGACCAG CTGACCATTA ACATAGATGA AGGTGGATGG GCTTGTCTGG 
     1381 TGTGCAAAGA GAAGCTCATT ATTTGGAAGA TTGCTCTGTC ACCTATTACT AAGTTATCCG 
     1441 TTTGCAAAGA ACTTCAGCTG CCACCTAGTG ATTTCCACTG GAGTGCCGAC TTAGTGGCTC 
     1501 TTTCTTACTC TTCTCCCTCA GGTGAAGCAC ATTCTACTCA GGCTGTTGCT GTCATGGTTG 
     1561 CCACCAGAGA AGGATCTATC CGCTATTGGC CAAGCCTTGC TGGTGAAGAT ACCTACACAG 
     1621 AGGCTTTTGT AGATTCGGGA GGTGATAAGA CTTACAGTTT CCTAACAGCA GTGCAGGGAG 
     1681 GAAGTTTTAT TTTGTCTTCA TCAGGAAGCC AACTAATTCG GTTGATACCT GAGAGCTCAG 
     1741 GAAAGATTCA TCAGCATATC CTGCCTCAGG GGCAAGGCAT GCTTTCAGGA ATTGGTCGAA 
     1801 AAGTTTCTTC TCTTTTTGGA ATTTTATCTC CTAGTAGTGA TCTCACACTT TCAAGTGTTC 
     1861 TCTGGGATAG AGAGAGATCA AGCTTTTATA GCCTGACGAG TTCAAACATC AGTAAATGGG 
     1921 AATTAGATGA TTCTTCAGAA AAGCATGCAT ACAGTTGGGA TATAAATAGA GCCCTGAAGG 
     1981 AAAACATTAC CGATGCTATT TGGGGATCTG AAAGTAACTA TGAAGCTATT AAAGAAGGAG 
     2041 TCAACATTCG ATATTTGGAC TTTAAGCAAA ACTGTGATGG GCTGGTGATT TTGGCAGCAG 
     2101 CATGGCACTC AGCAGACAAT CCATGTCTCA TCTATTACTC TCTGATAACA ATAGAAGATA 
     2161 ATGGTTGCCA AATGTCAGAT GCAGTTACTG TAGAAGTCAC TCAATATAAT CCACCTTTTC 
     2221 AGTCTGAAGA CCTGATTTTG TGTCAGTTGA CGGTCCCAAA CTTTTCAAAC CAGACTGCCT 
     2281 ATCTGTATAA CGAAAGTGCT GTCTATGTGT GCTCCACAGG AACTGGGAAA TTTTCTCTTC 
     2341 CCCAGGAGAA AATTGTCTTT AATGCACAAG GAGATAGTGT TTTAGGTGCT GGTGCCTGTG 
     2401 GTGGTGTTCC TATCATTTTT TCTAGAAACA GTGGACTGGT GTCTATTACT TCAAGGGAAA 
     2461 ATGTGTCTAT ATTGGCAGAA GACTTGGAAG GGTCTTTAGC ATCTTCAGTT GCTGGACCAA 
     2521 ACAGTGAGAG TATGATTTTT GAGACCACTA CAAAGAATGA AACTATAGCC CAGGAAGATA 
     2581 AAATCAAGTT GCTGAAAGCT GCCTTTCTGC AATACTGCAG AAAAGATTTA GGTCATGCTC 
     2641 AAATGGTGGT TGATGAGCTC TTTTCCTCTC ACTCTGATTT GGATTCTGAT TCTGAACTAG 
     2701 ACAGGGCAGT TACCCAAATC AGTGTAGACC TGATGGATGA CTACCCAGCA TCTGACCCAC 
     2761 GGTGGGCTGA GTCTGTCCCT GAGGAAGCAC CTGGGTTCAG CAATACGTCA CTGATTATCC 
     2821 TTCACCAGCT AGAAGACAAG ATGAAAGCTC ACTCTTTTCT TATGGACTTT ATTCATCAAG 
     2881 TTGGCTTATT TGGACGTCTA GGCAGTTTTC CAGTTAGAGG GACACCGATG GCCACTCGAC 
     2941 TGTTGCTCTG TGAGCATGCC GAAAAGCTGT CAGCCGCCAT TGTTCTCAAG AACCACCACT 
     3001 CCCGGCTTTC TGACCTTGTC AACACAGCCA TATTGATTGC TTTGAACAAG AGGGAGTATG 
     3061 AAATCCCATC CAACCTGACT CCTGCAGATG TCTTTTTCAG GGAGGTATCC CAAGTAGATA 
     3121 CCATCTGTGA GTGCTTACTG GAGCATGAGG AGCAAGTCTT GAGGGATGCA CCTATGGATT 
     3181 CCATTGAATG GGCTGAAGTG GTGATCAATG TGAACAATAT TCTCAAGGAT ATGCTGCAGG 
     3241 CTGCTAGTCA TTATCGCCAA AATAGAAACT CTTTGTATAG AAGAGAAGAA TCACTAGAAA 
     3301 AAGAACCTGA ATATGTTCCA TGGACGGCAA CAAGTGGTCC TGGTGGCATC CGAACGGTAA 
     3361 TAATACGCCA GCATGAGATT GTCCTGAAGG TGGCTTATCC ACAGGCAGAC AGCAACCTCC 
     3421 GAAACATCGT GACCGAGCAG CTGGTAGCCC TGATCGATTG CTTCCTGGAT GGTTATGTTT 
     3481 CTCAGCTTAA GTCTGTGGAT AAATCCAGTA ATCGGGAAAG ATATGACAAT CTGGAGATGG 
     3541 AATACCTACA GAAAAGATCA GATCTCTTAT CTCCTCTTCT TTCACTAGGC CAGTACCTGT 
     3601 GGGCTGCTTC TCTAGCAGAG AAATACTGTG ACTTTGATAT ATTGGTACAA ATGTGTGAGC 
     3661 AGACTGACAA CCAGAGCCGA CTCCAGCGCT ACATGACCCA GTTTGCTGAT CAGAATTTTT 
     3721 CAGACTTTCT CTTCCGTTGG TATCTGGAGA AAGGAAAGCG AGGCAAATTA TTATCTCAGC 
     3781 CCATTTCTCA GCATGGACAG TTGGCAAATT TTTTGCAAGC TCATGAACAT CTCAGCTGGT 
     3841 TACATGAAAT TAATAGCCAA GAATTAGAAA AGGCTCATGC AACACTTCTG GGTTTGGCAA 
     3901 ATATGGAAAC TCGTTACTTT GCAAAGAAGA AAACCCTTCT TGGCTTGAGT AAATTGGCTG 
     3961 CATTAGCTTC AGACTTTTCA GAGGATATGC TACAAGAAAA AATTGAAGAA ATGGCTGAGC 
     4021 AGGAGCGCTT TCTACTGCAT CAGGAGACCC TACCTGAACA GCTGCTGGCG GAGAAACAGC 
     4081 TAAATCTCAG TGCGATGCCA GTATTGACTG CACCACAACT CATTGGTCTA TATATCTGTG 
     4141 AAGAAAATAG AAGAGCTAAT GAATATGATT TCAAGAAAGC TTTGGACTTG TTGGAATATA 
     4201 TTGATGAGGA AGAAGATATA AATATAAATG ATCTAAAACT GGAAATCCTT TGCAAAGCTC 
     4261 TTCAGAGAGA TAACTGGTCC AGTTCTGATG GCAAAGATGA TCCAATTGAA GTATCTAAAG 
     4321 ACAGTATATT TGTGAAGATC TTACAGAAAC TTTTAAAAGA TGGCATTCAG CTCAGTGAGT 
     4381 ACTTACCGGA GGTGAAAGAC CTGCTACAAG CGGATCAGCT TGGAAGCTTA AAGTCCAATC 
     4441 CTTACTTCGA GTTTGTTTTG AAAGCAAATT ATGAATATTA TGTTCAGGGA CAAATAACGC 
     4501 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4561 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4621 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4681 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4741 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4801 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4861 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4921 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4981 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5041 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5101 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5161 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5221 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5281 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5341 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5401 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5461 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5521 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5581 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5641 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5701 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5761 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5821 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5881 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5941 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6001 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6061 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6121 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6181 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6241 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6301 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6361 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6421 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6481 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6541 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6601 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6661 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6721 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6781 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6841 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6901 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6961 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7021 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7081 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7141 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7201 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7261 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7321 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7381 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7441 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7501 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7561 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7621 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7681 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7741 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7801 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7861 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7921 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7981 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8041 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8101 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8161 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8221 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8281 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8341 GCGAATTTT
//