LOCUS       RC204175                8355 bp    DNA     circular  SYN 
DEFINITION  HEPH (Myc-DDK-tagged)-Human hephaestin (HEPH), transcript variant 2
ACCESSION   NM_014799
VERSION     NM_014799.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4512..4603 
                     /label="Myc-DDK"  
     misc_feature    4633..5218 
                     /label="PolyA signal"  
     misc_feature    4690..4709 
                     /label="Restriction Enzyme"  
     misc_feature    5494..6166 
                     /label="ColE1"  
     misc_feature    6596..7390 
                     /label="Kan/Neo"  
     misc_feature    7425..7727 
                     /label="SV40 ori"  
     misc_feature    7813..8290 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4494..4500 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4494 
                     /label="HEPH"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTCAGGC CACCTCCTCT GGGCTCTGCT GTTCATGCAG TCCTTGTGGC 
     1081 CTCAACTGAC TGATGGAGCC ACTCGAGTCT ACTACCTGGG CATCCGGGAT GTGCAGTGGA 
     1141 ACTATGCTCC CAAGGGAAGA AATGTCATCA CGAACCAGCC TCTGGACAGT GACATAGTGG 
     1201 CTTCCAGCTT CTTAAAGTCT GACAAGAACC GGATAGGGGG AACCTACAAG AAGACCATCT 
     1261 ATAAAGAATA CAAGGATGAC TCATACACAG ATGAAGTGGC CCAGCCTGCC TGGTTGGGCT 
     1321 TCCTGGGGCC AGTGTTGCAG GCTGAAGTGG GGGATGTCAT TCTTATTCAC CTGAAGAATT 
     1381 TTGCCACTCG TCCCTATACC ATCCACCCTC ATGGTGTCTT CTACGAGAAG GACTCTGAAG 
     1441 GTTCCCTATA CCCAGATGGC TCCTCTGGGC CACTGAAAGC TGATGACTCT GTTCCCCCGG 
     1501 GGGGCAGCCA TATCTACAAC TGGACCATTC CAGAAGGCCA TGCACCCACC GATGCTGACC 
     1561 CAGCGTGCCT CACCTGGATC TACCATTCTC ATGTAGATGC TCCACGAGAC ATTGCAACTG 
     1621 GCCTAATTGG GCCTCTCATC ACCTGTAAAA GAGGAGCCCT GGATGGGAAC TCCCCTCCTC 
     1681 AACGCCAGGA TGTAGACCAT GATTTCTTCC TCCTCTTCAG TGTGGTAGAT GAGAACCTCA 
     1741 GCTGGCATCT CAATGAGAAC ATTGCCACTT ACTGCTCAGA TCCTGCTTCA GTGGACAAAG 
     1801 AAGATGAGAC ATTTCAGGAG AGCAATAGGA TGCATGCAAT CAATGGCTTT GTTTTTGGGA 
     1861 ATTTACCTGA GCTGAACATG TGTGCACAGA AACGTGTGGC CTGGCACTTG TTTGGCATGG 
     1921 GCAATGAAAT TGATGTCCAC ACAGCATTTT TCCATGGACA GATGCTGACT ACCCGTGGAC 
     1981 ACCACACTGA TGTGGCTAAC ATCTTTCCAG CCACCTTTGT GACTGCTGAG ATGGTGCCCT 
     2041 GGGAACCTGG TACCTGGTTA ATTAGCTGCC AAGTGAACAG TCACTTTCGA GATGGCATGC 
     2101 AGGCACTCTA CAAGGTCAAG TCTTGCTCCA TGGCCCCTCC TGTGGACCTG CTCACAGGCA 
     2161 AAGTTCGACA GTACTTCATT GAGGCCCATG AGATTCAATG GGACTATGGC CCGATGGGGC 
     2221 ATGATGGGAG TACTGGGAAG AATTTGAGAG AGCCAGGCAG TATCTCAGAT AAGTTTTTCC 
     2281 AGAAGAGCTC CAGCCGAATT GGGGGCACTT ACTGGAAAGT GCGATATGAA GCCTTTCAAG 
     2341 ATGAGACATT CCAAGAGAAG ATGCATTTGG AGGAAGATAG GCATCTTGGA ATCCTGGGGC 
     2401 CAGTGATCCG GGCTGAGGTG GGTGACACCA TTCAGGTGGT CTTCTACAAC CGTGCCTCCC 
     2461 AGCCATTCAG CATGCAGCCC CATGGGGTCT TTTATGAGAA AGACTATGAA GGCACTGTGT 
     2521 ACAATGATGG CTCATCTTAC CCTGGCTTGG TTGCCAAGCC CTTTGAGAAA GTAACATACC 
     2581 GCTGGACAGT CCCCCCTCAT GCCGGTCCCA CTGCTCAGGA TCCTGCTTGT CTCACTTGGA 
     2641 TGTACTTCTC TGCTGCAGAT CCCATAAGAG ACACAAATTC TGGCCTGGTG GGCCCGCTGC 
     2701 TGGTGTGCAG GGCTGGTGCC TTGGGTGCAG ATGGCAAGCA GAAAGGGGTG GATAAAGAAT 
     2761 TCTTTCTTCT CTTCACTGTG TTGGATGAGA ACAAGAGCTG GTACAGCAAT GCCAATCAAG 
     2821 CAGCTGCTAT GTTGGATTTC CGACTGCTTT CAGAGGATAT TGAGGGCTTC CAAGACTCCA 
     2881 ATCGGATGCA TGCCATTAAT GGGTTTCTGT TCTCTAACCT GCCCAGGCTG GACATGTGCA 
     2941 AGGGTGACAC AGTGGCCTGG CACCTGCTCG GCCTGGGCAC AGAGACTGAT GTGCATGGAG 
     3001 TCATGTTCCA GGGCAACACT GTGCAGCTTC AGGGCATGAG GAAGGGTGCA GCTATGCTCT 
     3061 TTCCTCATAC CTTTGTCATG GCCATCATGC AGCCTGACAA CCTTGGGACA TTTGAGATTT 
     3121 ATTGCCAGGC AGGCAGCCAT CGAGAAGCAG GGATGAGGGC AATCTATAAT GTCTCCCAGT 
     3181 GTCCTGGCCA CCAAGCCACC CCTCGCCAAC GCTACCAAGC TGCAAGAATC TACTATATCA 
     3241 TGGCAGAAGA AGTAGAGTGG GACTATTGCC CTGACCGGAG CTGGGAACGG GAATGGCACA 
     3301 ACCAGTCTGA GAAGGACAGT TATGGTTACA TTTTCCTGAG CAACAAGGAT GGGCTCCTGG 
     3361 GTTCCAGATA CAAGAAAGCT GTATTCAGGG AATACACTGA TGGTACATTC AGGATCCCTC 
     3421 GGCCAAGGAC TGGACCAGAA GAACACTTGG GAATCTTGGG TCCACTTATC AAAGGTGAAG 
     3481 TTGGTGATAT CCTGACTGTG GTATTCAAGA ATAATGCCAG CCGCCCCTAC TCTGTGCATG 
     3541 CTCATGGAGT GCTAGAATCT ACTACTGTCT GGCCACTGGC TGCTGAGCCT GGTGAGGTGG 
     3601 TCACTTATCA GTGGAACATC CCAGAGAGGT CTGGCCCTGG GCCCAATGAC TCTGCTTGTG 
     3661 TTTCCTGGAT CTATTATTCT GCAGTGGATC CCATCAAGGA CATGTATAGT GGCCTGGTGG 
     3721 GGCCCTTGGC TATCTGCCAA AAGGGCATCC TGGAGCCCCA TGGAGGACGG AGTGACATGG 
     3781 ATCGGGAATT TGCATTGTTG TTCTTGATTT TTGATGAAAA TAAGTCTTGG TATTTGGAGG 
     3841 AAAATGTGGC AACCCATGGG TCCCAGGATC CAGGCAGTAT TAACCTACAG GATGAAACTT 
     3901 TCTTGGAGAG CAATAAAATG CATGCAATCA ATGGGAAACT CTATGCCAAC CTTAGGGGTC 
     3961 TTACCATGTA CCAAGGAGAA CGAGTGGCCT GGTACATGCT GGCCATGGGC CAAGATGTGG 
     4021 ATCTACACAC CATCCACTTT CATGCAGAGA GCTTCCTCTA TCGGAATGGC GAGAACTACC 
     4081 GGGCAGATGT GGTGGATCTG TTCCCAGGGA CTTTTGAGGT TGTGGAGATG GTGGCCAGCA 
     4141 ACCCTGGGAC ATGGCTGATG CACTGCCATG TGACTGACCA TGTCCATGCT GGCATGGAGA 
     4201 CCCTCTTCAC TGTTTTTTCT CGAACAGAAC ACTTAAGCCC TCTCACCGTC ATCACCAAAG 
     4261 AGACTGAAAA AGCAGTGCCC CCCAGAGACA TTGAAGAAGG CAATGTGAAG ATGCTGGGCA 
     4321 TGCAGATCCC CATAAAGAAT GTTGAGATGC TGGCCTCTGT TTTGGTTGCC ATTAGTGTCA 
     4381 CCCTTCTGCT CGTTGTTCTG GCTCTTGGTG GAGTGGTTTG GTACCAACAT CGACAGAGAA 
     4441 AGCTACGACG CAATAGGAGG TCCATCCTGG ATGACAGCTT CAAGCTTCTG TCTTTCAAAC 
     4501 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4561 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4621 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4681 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4741 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4801 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4861 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4921 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4981 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5041 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5101 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5161 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5221 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5281 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5341 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5401 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5461 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5521 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5581 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5641 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5701 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5761 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5821 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5881 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5941 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6001 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6061 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6121 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6181 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6241 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6301 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6361 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6421 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6481 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6541 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6601 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6661 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6721 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6781 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6841 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6901 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6961 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7021 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7081 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7141 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7201 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7261 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7321 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7381 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7441 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7501 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7561 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7621 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7681 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7741 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7801 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7861 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7921 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7981 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8041 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8101 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8161 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8221 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8281 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8341 TTTAACGCGA ATTTT
//