LOCUS       RC204121                6502 bp    DNA     circular  SYN 
DEFINITION  CNOT2 (Myc-DDK-tagged)-Human CCR4-NOT transcription complex, subunit 2 (CNOT2), transcript variant 2
ACCESSION   NM_014515
VERSION     NM_014515.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2659..2750 
                     /label="Myc-DDK"  
     misc_feature    2780..3365 
                     /label="PolyA signal"  
     misc_feature    2837..2856 
                     /label="Restriction Enzyme"  
     misc_feature    3641..4313 
                     /label="ColE1"  
     misc_feature    4743..5537 
                     /label="Kan/Neo"  
     misc_feature    5572..5874 
                     /label="SV40 ori"  
     misc_feature    5960..6437 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2641..2647 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2641 
                     /label="CNOT2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGAGGACT GATGGACATA CATTATCTGA GAAAAGAAAC TACCAGGTGA 
     1081 CAAACAGCAT GTTTGGTGCT TCAAGAAAGA AGTTTGTAGA GGGGGTCGAC AGTGACTACC 
     1141 ATGACGAAAA CATGTACTAC AGCCAGTCTT CTATGTTTCC ACATCGGTCA GAAAAAGATA 
     1201 TGCTGGCATC ACCATCTACA TCAGGTCAGC TGTCTCAGTT TGGGGCAAGT TTATACGGGC 
     1261 AACAAAGTGC ACTAGGCCTT CCAATGAGGG GGATGAGCAA CAATACCCCT CAGTTAAATC 
     1321 GCAGCTTATC ACAAGGCACT CAGTTACCGA GCCACGTCAC GCCAACAACA GGGGTACCAA 
     1381 CAATGTCACT TCACACGCCT CCATCTCCAA GCAGGGGTAT TTTGCCTATG AATCCTAGGA 
     1441 ATATGATGAA CCACTCCCAG GTTGGTCAGG GCATTGGAAT TCCTAGCAGG ACAAATAGCA 
     1501 TGAGCAGTTC AGGGTTAGGT AGCCCCAACA GAAGCTCGCC AAGCATAATA TGTATGCCAA 
     1561 AGCAGCAGCC TTCTCGACAG CCTTTTACTG TGAACAGTAT GTCTGGATTT GGAATGAACA 
     1621 GGAATCAGGC ATTTGGAATG AATAACTCCT TATCAAGTAA CATTTTTAAT GGAACAGACG 
     1681 GAAGTGAAAA TGTGACAGGA TTGGACCTTT CAGATTTCCC AGCATTAGCA GACCGAAACA 
     1741 GGAGGGAAGG AAGTGGTAAC CCAACTCCAT TAATAAACCC CTTGGCTGGA AGAGCTCCTT 
     1801 ATGTTGGAAT GGTAACAAAA CCAGCAAATG AACAATCCCA GGACTTCTCA ATACACAATG 
     1861 AAGATTTTCC AGCATTACCA GGCTCCAGCT ATAAAGATCC AACATCAAGT AATGATGACA 
     1921 GTAAATCTAA TTTGAATACA TCTGGCAAGA CAACTTCAAG TACAGATGGA CCCAAATTCC 
     1981 CTGGAGATAA AAGTTCAACA ACACAAAATA ATAACCAGCA GAAAAAAGGG ATCCAGGTGT 
     2041 TACCTGATGG TCGGGTTACT AACATTCCTC AAGGGATGGT GACGGACCAA TTTGGAATGA 
     2101 TTGGCCTGTT AACATTTATC AGGGCAGCAG AGACAGACCC AGGAATGGTA CATCTTGCAT 
     2161 TAGGAAGTGA CTTAACAACA TTAGGCCTCA ATCTGAACTC TCCTGAAAAT CTCTACCCCA 
     2221 AATTTGCGTC ACCCTGGGCA TCTTCACCTT GTCGACCTCA AGACATAGAC TTCCATGTTC 
     2281 CATCTGAGTA CTTAACGAAC ATTCACATTA GGGATAAGCT GGCTGCAATA AAACTTGGCC 
     2341 GATATGGTGA AGACCTTCTC TTCTATCTCT ATTACATGAA TGGAGGAGAC GTATTACAAC 
     2401 TTTTAGCTGC AGTGGAGCTT TTTAACCGTG ATTGGAGATA CCACAAAGAA GAACGAGTAT 
     2461 GGATTACCAG GGCACCAGGC ATGGAGCCAA CAATGAAAAC CAATACCTAT GAGAGGGGAA 
     2521 CATATTACTT CTTTGACTGT CTTAACTGGA GGAAAGTAGC TAAGGAGTTC CATCTGGAAT 
     2581 ATGACAAATT AGAAGAACGG CCTCACCTGC CATCCACCTT CAACTACAAC CCTGCTCAGC 
     2641 AAGCCTTCTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA 
     2701 GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT 
     2761 CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT 
     2821 CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG 
     2881 CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG 
     2941 GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG 
     3001 CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT 
     3061 TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA 
     3121 ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC 
     3181 CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC 
     3241 CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA 
     3301 GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG 
     3361 CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC 
     3421 AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 
     3481 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 
     3541 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 
     3601 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 
     3661 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 
     3721 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 
     3781 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 
     3841 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 
     3901 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 
     3961 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 
     4021 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 
     4081 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     4141 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     4201 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     4261 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC 
     4321 CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG 
     4381 GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG 
     4441 AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT 
     4501 TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG 
     4561 TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA 
     4621 GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG 
     4681 CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC 
     4741 CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC 
     4801 GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC 
     4861 AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC 
     4921 ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC 
     4981 GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG 
     5041 TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC 
     5101 TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT 
     5161 AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC 
     5221 AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC 
     5281 CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG 
     5341 CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT 
     5401 GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC 
     5461 GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC 
     5521 TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT 
     5581 CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG 
     5641 CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT 
     5701 GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG 
     5761 CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC 
     5821 CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC 
     5881 CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG 
     5941 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 
     6001 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 
     6061 AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG 
     6121 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC 
     6181 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG 
     6241 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC 
     6301 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT 
     6361 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC 
     6421 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA 
     6481 ACAAAAATTT AACGCGAATT TT
//