LOCUS       RC204067                7035 bp    DNA     circular  SYN 
DEFINITION  EXT2 (Myc-DDK-tagged)-Human exostosin 2 (EXT2), transcript variant 2
ACCESSION   NM_207122
VERSION     NM_207122.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3192..3283 
                     /label="Myc-DDK"  
     misc_feature    3313..3898 
                     /label="PolyA signal"  
     misc_feature    3370..3389 
                     /label="Restriction Enzyme"  
     misc_feature    4174..4846 
                     /label="ColE1"  
     misc_feature    5276..6070 
                     /label="Kan/Neo"  
     misc_feature    6105..6407 
                     /label="SV40 ori"  
     misc_feature    6493..6970 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3174..3180 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3174 
                     /label="EXT2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGTGCGTCG GTCAAGTATA ATATCCGGGG TCCTGCCCTC ATCCCAAGAA 
     1081 TGAAGACCAA GCACCGAATC TACTATATCA CCCTCTTCTC CATTGTCCTC CTGGGCCTCA 
     1141 TTGCCACTGG CATGTTTCAG TTTTGGCCCC ATTCTATCGA GTCCTCAAAT GACTGGAATG 
     1201 TAGAGAAGCG CAGCATCCGT GATGTGCCGG TTGTTAGGCT GCCAGCCGAC AGTCCCATCC 
     1261 CAGAGCGGGG GGATCTCAGT TGCAGAATGC ACACGTGTTT TGATGTCTAT CGCTGTGGCT 
     1321 TCAACCCAAA GAACAAAATC AAGGTGTATA TCTATGCTCT GAAAAAGTAC GTGGATGACT 
     1381 TTGGCGTCTC TGTCAGCAAC ACCATCTCCC GGGAGTATAA TGAACTGCTC ATGGCCATCT 
     1441 CAGACAGTGA CTACTACACT GATGACATCA ACCGGGCCTG TCTGTTTGTT CCCTCCATCG 
     1501 ATGTGCTTAA CCAGAACACA CTGCGCATCA AGGAGACAGC ACAAGCGATG GCCCAGCTCT 
     1561 CTAGGTGGGA TCGAGGTACG AATCACCTGT TGTTCAACAT GTTGCCTGGA GGTCCCCCAG 
     1621 ATTATAACAC AGCCCTGGAT GTCCCCAGAG ACAGGGCCCT GTTGGCTGGT GGCGGCTTTT 
     1681 CTACGTGGAC TTACCGGCAA GGCTACGATG TCAGCATTCC TGTCTATAGT CCACTGTCAG 
     1741 CTGAGGTGGA TCTTCCAGAG AAAGGACCAG GTCCACGGCA ATACTTCCTC CTGTCATCTC 
     1801 AGGTGGGTCT CCATCCTGAG TACAGAGAGG ACCTAGAAGC CCTCCAGGTC AAACATGGAG 
     1861 AGTCAGTGTT AGTACTCGAT AAATGCACCA ACCTCTCAGA GGGTGTCCTT TCTGTCCGTA 
     1921 AGCGCTGCCA CAAGCACCAG GTCTTCGATT ACCCACAGGT GCTACAGGAG GCTACTTTCT 
     1981 GTGTGGTTCT TCGTGGAGCT CGGCTGGGCC AGGCAGTATT GAGCGATGTG TTACAAGCTG 
     2041 GCTGTGTCCC GGTTGTCATT GCAGACTCCT ATATTTTGCC TTTCTCTGAA GTTCTTGACT 
     2101 GGAAGAGAGC ATCTGTGGTT GTACCAGAAG AAAAGATGTC AGATGTGTAC AGTATTTTGC 
     2161 AGAGCATCCC CCAAAGACAG ATTGAAGAAA TGCAGAGACA GGCCCGGTGG TTCTGGGAAG 
     2221 CGTACTTCCA GTCAATTAAA GCCATTGCCC TGGCCACCCT GCAGATTATC AATGACCGGA 
     2281 TCTATCCATA TGCTGCCATC TCCTATGAAG AATGGAATGA CCCTCCTGCT GTGAAGTGGG 
     2341 GCAGCGTGAG CAATCCACTC TTCCTCCCGC TGATCCCACC ACAGTCTCAA GGGTTCACCG 
     2401 CCATAGTCCT CACCTACGAC CGAGTAGAGA GCCTCTTCCG GGTCATCACT GAAGTGTCCA 
     2461 AGGTGCCCAG TCTATCCAAA CTACTTGTCG TCTGGAATAA TCAGAATAAA AACCCTCCAG 
     2521 AAGATTCTCT CTGGCCCAAA ATCCGGGTTC CATTAAAAGT TGTGAGGACT GCTGAAAACA 
     2581 AGTTAAGTAA CCGTTTCTTC CCTTATGATG AAATCGAGAC AGAAGCTGTT CTGGCCATTG 
     2641 ATGATGATAT CATTATGCTG ACCTCTGACG AGCTGCAATT TGGTTATGAG GTCTGGCGGG 
     2701 AATTTCCTGA CCGGTTGGTG GGTTACCCGG GTCGTCTGCA TCTCTGGGAC CATGAGATGA 
     2761 ATAAGTGGAA GTATGAGTCT GAGTGGACGA ATGAAGTGTC CATGGTGCTC ACTGGGGCAG 
     2821 CTTTTTATCA CAAGTATTTT AATTACCTGT ATACCTACAA AATGCCTGGG GATATCAAGA 
     2881 ACTGGGTAGA TGCTCATATG AACTGTGAAG ATATTGCCAT GAACTTCCTG GTGGCCAACG 
     2941 TCACGGGAAA AGCAGTTATC AAGGTAACCC CACGAAAGAA ATTCAAGTGT CCTGAGTGCA 
     3001 CAGCCATAGA TGGGCTTTCA CTAGACCAAA CACACATGGT GGAGAGGTCA GAGTGCATCA 
     3061 ACAAGTTTGC TTCAGTCTTC GGGACCATGC CTCTCAAGGT GGTGGAACAC CGAGCTGACC 
     3121 CTGTCCTGTA CAAAGATGAC TTTCCTGAGA AGCTGAAGAG CTTCCCCAAC ATTGGCAGCT 
     3181 TAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3241 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3301 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3361 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3421 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3481 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3541 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3601 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3661 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     3721 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     3781 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     3841 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     3901 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     3961 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4021 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4081 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4141 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4201 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4261 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4321 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4381 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4441 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4501 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4561 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     4621 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     4681 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     4741 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     4801 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     4861 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     4921 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     4981 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5041 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5101 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5161 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5221 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5281 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5341 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5401 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5461 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5521 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     5581 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     5641 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     5701 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     5761 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     5821 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     5881 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     5941 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6001 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6061 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6121 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6181 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6241 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6301 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6361 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6421 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6481 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6541 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     6601 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     6661 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     6721 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     6781 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     6841 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     6901 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     6961 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7021 TTTAACGCGA ATTTT
//