LOCUS       RC203930                8496 bp    DNA     circular  SYN 
DEFINITION  PHLPP1 (Myc-DDK-tagged)-Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1)
ACCESSION   NM_194449
VERSION     NM_194449.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4653..4744 
                     /label="Myc-DDK"  
     misc_feature    4774..5359 
                     /label="PolyA signal"  
     misc_feature    4831..4850 
                     /label="Restriction Enzyme"  
     misc_feature    5635..6307 
                     /label="ColE1"  
     misc_feature    6737..7531 
                     /label="Kan/Neo"  
     misc_feature    7566..7868 
                     /label="SV40 ori"  
     misc_feature    7954..8431 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4635..4641 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4635 
                     /label="PHLPP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTCGGAG ATTGGCTGCC TCATCCGCTT CTATGCAGGA AAACCTCACA 
     1081 GCACGGGTAG CTCTGAACGG ATTCAGCTCT CAGGAATGTA TAATGTCCGT AAAGGCAAGA 
     1141 TGCAGTTGCC AGTGAACCGA TGGACAAGAC GCCAAGTCAT CCTATGTGGG ACCTGCCTGA 
     1201 TAGTATCATC TGTGAAAGAC AGCTTGACCG GAAAGATGCA TGTTCTGCCA CTAATTGGTG 
     1261 GAAAAGTAGA AGAAGTGAAA AAGCACCAAC ACTGTTTAGC ATTTAGCTCC TCTGGACCCC 
     1321 AAAGCCAGAC TTACTACATT TGCTTTGATA CTTTCACAGA ATACTTAAGG TGGCTGCGAC 
     1381 AAGTCTCCAA GGTTGCATCC CAGCGCATTA GCTCAGTGGA CCTCTCGTGT TGTAGCCTGG 
     1441 AACATCTGCC TGCCAACCTC TTCTACAGCC AAGACCTCAC TCATCTCAAT TTAAAACAAA 
     1501 ACTTCCTAAG GCAGAACCCT AGCCTTCCAG CTGCCAGGGG GCTTAATGAA CTGCAAAGGT 
     1561 TCACCAAGTT GAAGAGTCTT AACCTTTCCA ATAATCATTT AGGGGACTTC CCTCTGGCAG 
     1621 TCTGCAGTAT TCCAACCCTG GCAGAGCTGA ACGTGTCCTG CAATGCCCTG CGATCAGTCC 
     1681 CGGCAGCCGT TGGAGTGATG CACAACTTAC AGACATTTTT GTTGGATGGA AACTTTCTCC 
     1741 AATCCCTTCC TGCTGAGTTG GAGAACATGA AGCAGCTTAG TTATCTGGGT CTTTCTTTCA 
     1801 ATGAATTTAC TGACATTCCC GAAGTATTGG AGAAATTGAC TGCTGTGGAT AAACTTTGTA 
     1861 TGTCTGGAAA CTGTGTGGAG ACCCTTAGGC TACAGGCTTT AAGAAAAATG CCTCACATTA 
     1921 AACATGTGGA TCTAAGGTTG AACGTAATTA GGAAGCTGAT AGCAGATGAA GTGGACTTTC 
     1981 TACAGCATGT TACTCAGCTT GACCTACGAG ACAATAAGCT TGGTGATCTA GATGCTATGA 
     2041 TTTTCAACAA CATTGAAGTT TTACACTGTG AAAGGAATCA ACTGGTCACA TTAGACATCT 
     2101 GTGGCTATTT CCTAAAAGCG CTCTATGCCT CTTCTAATGA ACTTGTTCAA CTTGATGTTT 
     2161 ACCCAGTTCC AAATTATCTG TCCTACATGG ATGTTTCAAG GAACCGCTTA GAAAATGTGC 
     2221 CTGAGTGGGT ATGTGAAAGC CGAAAGCTAG AAGTTTTGGA TATTGGCCAT AATCAAATAT 
     2281 GTGAACTTCC TGCCCGCTTA TTTTGTAATA GCAGTCTCCG GAAACTACTG GCAGGACACA 
     2341 ACCAGTTGGC AAGGCTGCCT GAAAGGCTAG AAAGAACCTC GGTGGAGGTC TTGGATGTGC 
     2401 AACACAACCA GCTCCTTGAG CTCCCACCTA ACCTTCTGAT GAAGGCTGAC AGCCTGAGAT 
     2461 TCCTGAACGC CTCTGCGAAC AAACTGGAAA GCCTTCCTCC AGCCACGCTT TCCGAAGAGA 
     2521 CAAACAGTAT CTTACAAGAG TTGTATTTGA CAAATAACAG CCTCACAGAC AAATGTGTGC 
     2581 CCTTGTTAAC GGGACACCCC CATTTGAAGA TCCTTCACAT GGCCTATAAC CGACTTCAGA 
     2641 GTTTTCCAGC AAGTAAAATG GCGAAACTGG AGGAACTTGA AGAAATTGAT CTCAGTGGGA 
     2701 ATAAGCTGAA AGCCATCCCA ACAACGATCA TGAATTGCAG GCGCATGCAC ACCGTGATTG 
     2761 CTCACTCCAA CTGCATCGAG GTCTTTCCCG AAGTTATGCA GCTCCCAGAG ATCAAGTGTG 
     2821 TGGACCTGAG CTGTAATGAG CTAAGTGAAG TCACATTACC AGAAAACCTG CCTCCCAAAC 
     2881 TGCAGGAGCT AGACCTGACT GGAAACCCGC GCCTTGTCCT TGATCACAAA ACCCTGGAAC 
     2941 TACTGAATAA TATCCGCTGT TTCAAGATTG ATCAGCCTTC TACAGGAGAC GCTTCCGGAG 
     3001 CCCCAGCTGT ATGGAGTCAT GGTTACACTG AAGCTTCGGG GGTAAAAAAC AAGTTGTGTG 
     3061 TCGCAGCCCT GTCGGTGAAT AACTTCTGTG ACAACCGCGA AGCCCTGTAT GGTGTGTTTG 
     3121 ACGGAGACCG GAATGTGGAG GTGCCCTACC TTCTCCAGTG CACTATGAGT GACATTTTGG 
     3181 CTGAAGAGCT GCAAAAAACA AAAAACGAAG AAGAATACAT GGTCAATACA TTCATTGTCA 
     3241 TGCAAAGGAA ACTTGGAACT GCTGGGCAGA AGCTTGGTGG TGCCGCTGTC CTTTGTCATA 
     3301 TCAAGCATGA CCCTGTGGAT CCAGGAGGAT CCTTCACCTT GACCTCTGCT AATGTGGGCA 
     3361 AGTGCCAAAC AGTTCTCTGT CGAAATGGAA AGCCGCTGCC TCTGTCCAGA TCTTACATCA 
     3421 TGAGCTGTGA AGAAGAGCTG AAGAGGATTA AACAGCACAA GGCCATTATC ACTGAGGATG 
     3481 GCAAGGTGAA CGGAGTGACT GAGTCCACGC GCATCCTGGG CTACACCTTC CTCCATCCCA 
     3541 GTGTGGTGCC TCGCCCCCAC GTGCAGTCCG TGCTCCTGAC TCCCCAGGAT GAGTTCTTCA 
     3601 TCCTAGGCAG TAAGGGGTTG TGGGACAGCC TGTCCGTCGA GGAGGCCGTG GAAGCCGTGC 
     3661 GCAACGTGCC CGATGCCCTG GCTGCTGCCA AGAAGCTGTG TACCCTGGCC CAGAGCTACG 
     3721 GCTGCCACGA CAGCATCAGC GCTGTGGTGG TGCAGCTCAG TGTCACTGAG GACAGCTTCT 
     3781 GCTGCTGCGA GCTCAGCGCC GGTGGGGCTG TGCCACCACC CAGTCCTGGC ATCTTTCCTC 
     3841 CCTCAGTGAA CATGGTGATC AAGGATCGGC CCTCAGATGG GCTGGGCGTG CCGTCCTCCA 
     3901 GCAGCGGCAT GGCTTCCGAG ATTAGCAGTG AGCTCTCCAC TTCTGAGATG AGCAGCGAGG 
     3961 TGGGGTCAAC AGCCTCCGAT GAGCCCCCGC CCGGAGCCCT AAGCGAGAAC AGCCCTGCCT 
     4021 ACCCCAGTGA GCAGCGCTGC ATGCTCCACC CCATCTGTCT GTCCAACTCC TTCCAGCGCC 
     4081 AGCTATCCAG CGCCACGTTC TCTAGCGCCT TCTCCGACAA CGGCCTTGAC AGTGACGATG 
     4141 AGGAGCCCAT CGAGGGCGTC TTCACCAACG GCAGCCGGGT GGAGGTGGAG GTGGACATCC 
     4201 ACTGCAGCCG GGCCAAGGAG AAGGAGAAAC AGCAGCACCT GCTTCAGGTG CCAGCAGAGG 
     4261 CCAGTGATGA GGGCATTGTC ATCAGCGCCA ACGAGGATGA GCCAGGTCTG CCCAGGAAGG 
     4321 CAGACTTCTC TGCCGTTGGG ACCATTGGGC GCCGGAGGGC CAATGGCTCT GTTGCGCCCC 
     4381 AGGAAAGGAG CCACAATGTG ATAGAGGTGG CTACAGACGC ACCTCTTCGA AAGCCTGGAG 
     4441 GCTATTTTGC TGCCCCGGCT CAGCCGGATC CTGATGATCA GTTTATCATA CCCCCGGAGC 
     4501 TGGAAGAGGA GGTCAAAGAA ATCATGAAGC ATCACCAGGA GCAACAGCAG CAGCAGCAGC 
     4561 CGCCACCACC CCCTCAGCTC CAGCCGCAGC TGCCGCGGCA CTACCAGCTG GACCAGCTGC 
     4621 CAGATTATTA CGACACGCCA CTAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4681 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4741 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4801 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4861 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4921 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4981 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5041 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5101 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5161 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5221 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5281 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5341 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5401 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5461 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5521 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5581 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5641 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5701 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5761 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5821 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5881 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5941 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6001 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6061 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6121 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6181 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6241 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6301 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6361 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6421 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6481 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6541 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6601 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6661 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6721 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6781 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6841 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6901 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6961 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7021 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7081 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7141 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7201 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7261 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7321 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7381 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7441 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7501 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7561 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7621 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7681 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7741 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7801 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7861 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7921 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7981 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8041 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8101 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8161 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8221 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8281 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8341 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8401 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8461 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//