LOCUS       RC203833                7692 bp    DNA     circular  SYN 
DEFINITION  MATN2 (Myc-DDK-tagged)-Human matrilin 2 (MATN2), transcript variant 2
ACCESSION   NM_030583
VERSION     NM_030583.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3849..3940 
                     /label="Myc-DDK"  
     misc_feature    3970..4555 
                     /label="PolyA signal"  
     misc_feature    4027..4046 
                     /label="Restriction Enzyme"  
     misc_feature    4831..5503 
                     /label="ColE1"  
     misc_feature    5933..6727 
                     /label="Kan/Neo"  
     misc_feature    6762..7064 
                     /label="SV40 ori"  
     misc_feature    7150..7627 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3831..3837 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3831 
                     /label="MATN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAAAGATG CTCGCAGGCT GCTTTCTGCT GATCCTCGGA CAGATCGTCC 
     1081 TCCTCCCTGC CGAGGCCAGG GAGCGGTCAC GTGGGAGGTC CATCTCTAGG GGCAGACACG 
     1141 CTCGGACCCA CCCGCAGACG GCCCTTCTGG AGAGTTCCTG TGAGAACAAG CGGGCAGACC 
     1201 TGGTTTTCAT CATTGACAGC TCTCGCAGTG TCAACACCCA TGACTATGCA AAGGTCAAGG 
     1261 AGTTCATCGT GGACATCTTG CAATTCTTGG ACATTGGTCC TGATGTCACC CGAGTGGGCC 
     1321 TGCTCCAATA TGGCAGCACT GTCAAGAATG AGTTCTCCCT CAAGACCTTC AAGAGGAAGT 
     1381 CCGAGGTGGA GCGTGCTGTC AAGAGGATGC GGCATCTGTC CACGGGCACC ATGACCGGGC 
     1441 TGGCCATCCA GTATGCCCTG AACATCGCAT TCTCAGAAGC AGAGGGGGCC CGGCCCCTGA 
     1501 GGGAGAATGT GCCACGGGTC ATAATGATCG TGACAGATGG GAGACCTCAG GACTCCGTGG 
     1561 CCGAGGTGGC TGCTAAGGCA CGGGACACGG GCATCCTAAT CTTTGCCATT GGTGTGGGCC 
     1621 AGGTAGACTT CAACACCTTG AAGTCCATTG GGAGTGAGCC CCATGAGGAC CATGTCTTCC 
     1681 TTGTGGCCAA TTTCAGCCAG ATTGAGACGC TGACCTCCGT GTTCCAGAAG AAGTTGTGCA 
     1741 CGGCCCACAT GTGCAGCACC CTGGAGCATA ACTGTGCCCA CTTCTGCATC AACATCCCTG 
     1801 GCTCATACGT CTGCAGGTGC AAACAAGGCT ACATTCTCAA CTCGGATCAG ACGACTTGCA 
     1861 GAATCCAGGA TCTGTGTGCC ATGGAGGACC ACAACTGTGA GCAGCTCTGT GTGAATGTGC 
     1921 CGGGCTCCTT CGTCTGCCAG TGCTACAGTG GCTACGCCCT GGCTGAGGAT GGGAAGAGGT 
     1981 GTGTGGCTGT GGACTACTGT GCCTCAGAAA ACCACGGATG TGAACATGAG TGTGTAAATG 
     2041 CTGATGGCTC CTACCTTTGC CAGTGCCATG AAGGATTTGC TCTTAACCCA GATGAAAAAA 
     2101 CGTGCACAAA GATAGACTAC TGTGCCTCAT CTAATCATGG ATGTCAGCAT GAGTGTGTTA 
     2161 ACACAGATGA TTCCTATTCC TGCCACTGCC TGAAAGGCTT TACCCTGAAT CCAGATAAGA 
     2221 AAACCTGCAG AAGGATCAAC TACTGTGCAC TGAACAAACC GGGCTGTGAG CATGAGTGCG 
     2281 TCAACATGGA GGAGAGCTAC TACTGCCGCT GCCACCGTGG CTACACTCTG GACCCCAATG 
     2341 GCAAAACCTG CAGCCGAGTG GACCACTGTG CACAGCAGGA CCATGGCTGT GAGCAGCTGT 
     2401 GTCTGAACAC GGAGGATTCC TTCGTCTGCC AGTGCTCAGA AGGCTTCCTC ATCAACGAGG 
     2461 ACCTCAAGAC CTGCTCCCGG GTGGATTACT GCCTGCTGAG TGACCATGGT TGTGAATACT 
     2521 CCTGTGTCAA CATGGACAGA TCCTTTGCCT GTCAGTGTCC TGAGGGACAC GTGCTCCGCA 
     2581 GCGATGGGAA GACGTGTGCA AAATTGGACT CTTGTGCTCT GGGGGACCAC GGTTGTGAAC 
     2641 ATTCGTGTGT AAGCAGTGAA GATTCGTTTG TGTGCCAGTG CTTTGAAGGT TATATACTCC 
     2701 GTGAAGATGG AAAAACCTGC AGAAGGAAAG ATGTCTGCCA AGCTATAGAC CATGGCTGTG 
     2761 AACACATTTG TGTGAACAGT GACGACTCAT ACACGTGCGA GTGCTTGGAG GGATTCCGGC 
     2821 TCGCTGAGGA TGGGAAACGC TGCCGAAGGA AGGATGTCTG CAAATCAACC CACCATGGCT 
     2881 GCGAACACAT TTGTGTTAAT AATGGGAATT CCTACATCTG CAAATGCTCA GAGGGATTTG 
     2941 TTCTAGCTGA GGACGGAAGA CGGTGCAAGA AATGCACTGA AGGCCCAATT GACCTGGTCT 
     3001 TTGTGATCGA TGGATCCAAG AGTCTTGGAG AAGAGAATTT TGAGGTCGTG AAGCAGTTTG 
     3061 TCACTGGAAT TATAGATTCC TTGACAATTT CCCCCAAAGC CGCTCGAGTG GGGCTGCTCC 
     3121 AGTATTCCAC ACAGGTCCAC ACAGAGTTCA CCCTGAGAAA CTTCAACTCA GCCAAAGACA 
     3181 TGAAAAAAGC CGTGGCCCAC ATGAAATACA TGGGAAAGGG CTCTATGACT GGGCTGGCCC 
     3241 TGAAACACAT GTTTGAGAGA AGTTTTACCC AAGGAGAAGG GGCCAGGCCC CTTTCCACAA 
     3301 GGGTGCCCAG AGCAGCCATT GTGTTCACCG ACGGACGGGC TCAGGATGAC GTCTCCGAGT 
     3361 GGGCCAGTAA AGCCAAGGCC AATGGTATCA CTATGTATGC TGTTGGGGTA GGAAAAGCCA 
     3421 TTGAGGAGGA ACTACAAGAG ATTGCCTCTG AGCCCACAAA CAAGCATCTC TTCTATGCCG 
     3481 AAGACTTCAG CACAATGGAT GAGATAAGTG AAAAACTCAA GAAAGGCATC TGTGAAGCTC 
     3541 TAGAAGACTC CGATGGAAGA CAGGACTCTC CAGCAGGGGA ACTGCCAAAA ACGGTCCAAC 
     3601 AGCCAACAGT GCAACACAGA TATCTGTTTG AAGAAGACAA TCTTTTACGG TCTACACAAA 
     3661 AGCTTTCCCA TTCAACAAAA CCTTCAGGAA GCCCTTTGGA AGAAAAACAC GATCAATGCA 
     3721 AATGTGAAAA CCTTATAATG TTCCAGAACC TTGCAAACGA AGAAGTAAGA AAATTAACAC 
     3781 AGCGCTTAGA AGAAATGACA CAGAGAATGG AAGCCCTGGA AAATCGCCTG AGATACAGAA 
     3841 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3901 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3961 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4021 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4081 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4141 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4201 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4261 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4321 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4381 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4441 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4501 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4561 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4621 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4681 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4741 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4801 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4861 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4921 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4981 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5041 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5101 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5161 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5221 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5281 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5341 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5401 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5461 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5521 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5581 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5641 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5701 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5761 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5821 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5881 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5941 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6001 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6061 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6121 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6181 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6241 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6301 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6361 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6421 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6481 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6541 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6601 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6661 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6721 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6781 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6841 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6901 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6961 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7021 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7081 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7141 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7201 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7261 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7321 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7381 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7441 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7501 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7561 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7621 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7681 AACGCGAATT TT
//