LOCUS       RC203766L2                8460 bp    DNA     circular  SYN 
DEFINITION  LPL (Myc-DDK-tagged)-Human lipoprotein lipase (LPL)
ACCESSION   NM_000237
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6751..6931 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    7659..8318 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    6979..7598 
                     /label="ColE1"  
     misc_feature    5626..5646 
                     /label="LR50"  
     misc_feature    4766..5491 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    5635..6226 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4745..4751 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4745 
                     /label="LPL"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAGAGCAAA GCCCTGCTCG TGCTGACTCT 
     3361 GGCCGTGTGG CTCCAGAGTC TGACCGCCTC CCGCGGAGGG GTGGCCGCCG CCGACCAAAG 
     3421 AAGAGATTTT ATCGACATCG AAAGTAAATT TGCCCTAAGG ACCCCTGAAG ACACAGCTGA 
     3481 GGACACTTGC CACCTCATTC CCGGAGTAGC AGAGTCCGTG GCTACCTGTC ATTTCAATCA 
     3541 CAGCAGCAAA ACCTTCATGG TGATCCATGG CTGGACGGTA ACAGGAATGT ATGAGAGTTG 
     3601 GGTGCCAAAA CTTGTGGCCG CCCTGTACAA GAGAGAACCA GACTCCAATG TCATTGTGGT 
     3661 GGACTGGCTG TCACGGGCTC AGGAGCATTA CCCAGTGTCC GCGGGCTACA CCAAACTGGT 
     3721 GGGACAGGAT GTGGCCCGGT TTATCAACTG GATGGAGGAG GAGTTTAACT ACCCTCTGGA 
     3781 CAATGTCCAT CTCTTGGGAT ACAGCCTTGG AGCCCATGCT GCTGGCATTG CAGGAAGTCT 
     3841 GACCAATAAG AAAGTCAACA GAATTACTGG CCTCGATCCA GCTGGACCTA ACTTTGAGTA 
     3901 TGCAGAAGCC CCGAGTCGTC TTTCTCCTGA TGATGCAGAT TTTGTAGACG TCTTACACAC 
     3961 ATTCACCAGA GGGTCCCCTG GTCGAAGCAT TGGAATCCAG AAACCAGTTG GGCATGTTGA 
     4021 CATTTACCCG AATGGAGGTA CTTTTCAGCC AGGATGTAAC ATTGGAGAAG CTATCCGCGT 
     4081 GATTGCAGAG AGAGGACTTG GAGATGTGGA CCAGCTAGTG AAGTGCTCCC ACGAGCGCTC 
     4141 CATTCATCTC TTCATCGACT CTCTGTTGAA TGAAGAAAAT CCAAGTAAGG CCTACAGGTG 
     4201 CAGTTCCAAG GAAGCCTTTG AGAAAGGGCT CTGCTTGAGT TGTAGAAAGA ACCGCTGCAA 
     4261 CAATCTGGGC TATGAGATCA ATAAAGTCAG AGCCAAAAGA AGCAGCAAAA TGTACCTGAA 
     4321 GACTCGTTCT CAGATGCCCT ACAAAGTCTT CCATTACCAA GTAAAGATTC ATTTTTCTGG 
     4381 GACTGAGAGT GAAACCCATA CCAATCAGGC CTTTGAGATT TCTCTGTATG GCACCGTGGC 
     4441 CGAGAGTGAG AACATCCCAT TCACTCTGCC TGAAGTTTCC ACAAATAAGA CCTACTCCTT 
     4501 CCTAATTTAC ACAGAGGTAG ATATTGGAGA ACTACTCATG TTGAAGCTCA AATGGAAGAG 
     4561 TGATTCATAC TTTAGCTGGT CAGACTGGTG GAGCAGTCCC GGCTTCGCCA TTCAGAAGAT 
     4621 CAGAGTAAAA GCAGGAGAGA CTCAGAAAAA GGTGATCTTC TGTTCTAGGG AGAAAGTGTC 
     4681 TCATTTGCAG AAAGGAAAGG CACCTGCGGT ATTTGTGAAA TGCCATGACA AGTCTCTGAA 
     4741 TAAGAAGTCA GGCACGCGTA CGCGGCCGCT CGAGATGAGC GGGGGCGAGG AGCTGTTCGC 
     4801 CGGCATCGTG CCCGTGCTGA TCGAGCTGGA CGGCGACGTG CACGGCCACA AGTTCAGCGT 
     4861 GCGCGGCGAG GGCGAGGGCG ACGCCGACTA CGGCAAGCTG GAGATCAAGT TCATCTGCAC 
     4921 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTGGTGACC ACCCTCTGCT ACGGCATCCA 
     4981 GTGCTTCGCC CGCTACCCCG AGCACATGAA GATGAACGAC TTCTTCAAGA GCGCCATGCC 
     5041 CGAGGGCTAC ATCCAGGAGC GCACCATCCA GTTCCAGGAC GACGGCAAGT ACAAGACCCG 
     5101 CGGCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCAAGGA 
     5161 CTTCAAGGAG GACGGCAACA TCCTGGGCCA CAAGCTGGAG TACAGCTTCA ACAGCCACAA 
     5221 CGTGTACATC CGCCCCGACA AGGCCAACAA CGGCCTGGAG GCTAACTTCA AGACCCGCCA 
     5281 CAACATCGAG GGCGGCGGCG TGCAGCTGGC CGACCACTAC CAGACCAACG TGCCCCTGGG 
     5341 CGACGGCCCC GTGCTGATCC CCATCAACCA CTACCTGAGC ACTCAGACCA AGATCAGCAA 
     5401 GGACCGCAAC GAGGCCCGCG ACCACATGGT GCTCCTGGAG TCCTTCAGCG CCTGCTGCCA 
     5461 CACCCACGGC ATGGACGAGC TGTACAGGTC CGGACTCAGA TAAGTTTAAA CGGCCGGCCG 
     5521 CGGTCTGTAC AAGTAGGATT CGTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC 
     5581 GAAGTTATAC ATGTTTAAGG GTTCCGGTTC CACTAGGTAC AATTCGATAT CAAGCTTATC 
     5641 GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT 
     5701 GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC 
     5761 CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG 
     5821 TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC 
     5881 ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC 
     5941 CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG 
     6001 CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG 
     6061 CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC 
     6121 CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT 
     6181 CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC 
     6241 CGTCGACCTC GATCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA ATACAGCAGC 
     6301 TACCAATGCT GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT 
     6361 CACACCTCAG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT 
     6421 TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATATCCT 
     6481 TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TGGCAGAACT ACACACCAGG 
     6541 GCCAGGGATC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA 
     6601 AGAGAAGGTA GAAGAAGCCA ATGAAGGAGA GAACACCCGC TTGTTACACC CTGTGAGCCT 
     6661 GCATGGGATG GATGACCCGG AGAGAGAAGT ATTAGAGTGG AGGTTTGACA GCCGCCTAGC 
     6721 ATTTCATCAC ATGGCCCGAG AGCTGCATCC GGACTGTACT GGGTCTCTCT GGTTAGACCA 
     6781 GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG 
     6841 CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG 
     6901 ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGCATGTGAG CAAAAGGCCA 
     6961 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     7021 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     7081 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     7141 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     7201 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     7261 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     7321 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     7381 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     7441 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     7501 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     7561 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     7621 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGATTA CGCCCCGCCC 
     7681 TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA 
     7741 CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA 
     7801 TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA 
     7861 AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT 
     7921 TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA 
     7981 AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA 
     8041 TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT 
     8101 GCCATACGGA ACTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA 
     8161 TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG 
     8221 GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC 
     8281 CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATACT CTTCCTTTTT 
     8341 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8401 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
//