LOCUS       RC203641L2                9609 bp    DNA     circular  SYN 
DEFINITION  LRRC8D (Myc-DDK-tagged)-Human leucine rich repeat containing 8 family, member D (LRRC8D), transcript variant 2
ACCESSION   NM_018103
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7900..8080 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8808..9467 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    8128..8747 
                     /label="ColE1"  
     misc_feature    6775..6795 
                     /label="LR50"  
     misc_feature    5915..6640 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6784..7375 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5894..5900 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5894 
                     /label="LRRC8D"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTTTACCCTT GCGGAAGTTG CATCACTTAA 
     3361 TGACATTCAG CCAACTTACC GAATCCTGAA ACCATGGTGG GATGTGTTTA TGGATTACCT 
     3421 AGCTGTTGTT ATGTTAATGG TAGCCATCTT TGCAGGAACC ATGCAACTTA CCAAAGATCA 
     3481 GGTGGTCTGT TTGCCAGTAT TGCCATCTCC TGTAAATTCA AAGGCACATA CACCACCAGG 
     3541 AAATGCCGAG GTCACCACCA ACATCCCAAA GATGGAAGCA GCCACCAACC AAGACCAAGA 
     3601 TGGGCGGACA ACAAACGACA TTTCCTTTGG GACATCTGCT GTGACACCTG ACATACCTCT 
     3661 CAGAGCCACA TATCCTCGCA CAGATTTCGC ACTTCCAAAT CAGGAGGCAA AGAAAGAGAA 
     3721 GAAAGATCCA ACAGGTCGAA AAACAAACTT GGATTTTCAG CAATATGTAT TTATTAATCA 
     3781 AATGTGTTAC CATCTGGCCC TTCCGTGGTA TTCTAAGTAC TTTCCATACC TAGCTCTTAT 
     3841 ACATACTATT ATTCTCATGG TCAGTAGCAA CTTTTGGTTC AAATATCCCA AAACATGCTC 
     3901 AAAAGTAGAA CATTTTGTTT CAATATTAGG AAAGTGCTTT GAATCCCCTT GGACGACAAA 
     3961 AGCGTTGTCT GAGACAGCAT GCGAAGACTC AGAGGAAAAC AAGCAGAGAA TAACAGGTGC 
     4021 CCAGACTCTA CCAAAGCATG TTTCTACCAG CAGTGATGAA GGGAGCCCCA GTGCCAGTAC 
     4081 ACCAATGATC AATAAAACTG GCTTTAAATT TTCAGCTGAG AAGCCTGTGA TTGAAGTTCC 
     4141 CAGCATGACA ATCCTGGATA AAAAGGATGG AGAGCAGGCC AAAGCCCTGT TTGAGAAAGT 
     4201 GAGGAAGTTC CGTGCCCATG TGGAAGATAG TGACTTGATC TATAAACTCT ATGTGGTCCA 
     4261 AACAGTTATC AAAACAGCCA AGTTCATTTT TATTCTCTGC TATACAGCGA ACTTTGTCAA 
     4321 CGCAATCAGC TTTGAACACG TCTGCAAGCC CAAAGTTGAG CATCTGATTG GTTATGAGGT 
     4381 ATTTGAGTGC ACCCACAATA TGGCTTACAT GTTGAAAAAG CTTCTCATCA GTTACATATC 
     4441 CATTATTTGT GTTTATGGCT TTATCTGCCT CTACACTCTC TTCTGGTTAT TCAGGATACC 
     4501 TTTGAAGGAA TATTCTTTCG AAAAAGTCAG AGAAGAGAGC AGTTTTAGTG ACATTCCAGA 
     4561 TGTCAAAAAC GATTTTGCGT TCCTTCTTCA CATGGTAGAC CAGTATGACC AGCTATATTC 
     4621 CAAGCGTTTT GGTGTGTTCT TGTCAGAAGT TAGTGAAAAT AAACTTAGGG AAATTAGTTT 
     4681 GAACCATGAG TGGACATTTG AAAAACTCAG GCAGCACATT TCACGCAACG CCCAGGACAA 
     4741 GCAGGAGTTG CATCTGTTCA TGCTGTCGGG GGTGCCCGAT GCTGTCTTTG ACCTCACAGA 
     4801 CCTGGATGTG CTAAAGCTTG AACTAATTCC AGAAGCTAAA ATTCCTGCTA AGATTTCTCA 
     4861 AATGACTAAC CTCCAAGAGC TCCACCTCTG CCACTGCCCT GCAAAAGTTG AACAGACTGC 
     4921 TTTTAGCTTT CTTCGCGATC ACTTGAGATG CCTTCACGTG AAGTTCACTG ATGTGGCTGA 
     4981 AATTCCTGCC TGGGTGTATT TGCTCAAAAA CCTTCGAGAG TTGTACTTAA TAGGCAATTT 
     5041 GAACTCTGAA AACAATAAGA TGATAGGACT TGAATCTCTC CGAGAGTTGC GGCACCTTAA 
     5101 GATTCTCCAC GTGAAGAGCA ATTTGACCAA AGTTCCCTCC AACATTACAG ATGTGGCTCC 
     5161 ACATCTTACA AAGTTAGTCA TTCATAATGA CGGCACTAAA CTCTTGGTAC TGAACAGCCT 
     5221 TAAGAAAATG ATGAATGTCG CTGAGCTGGA ACTCCAGAAC TGTGAGCTAG AGAGAATCCC 
     5281 ACATGCTATT TTCAGCCTCT CTAATTTACA GGAACTGGAT TTAAAGTCCA ATAACATTCG 
     5341 CACAATTGAG GAAATCATCA GTTTCCAGCA TTTAAAACGA CTGACTTGTT TAAAATTATG 
     5401 GCATAACAAA ATTGTTACTA TTCCTCCCTC TATTACCCAT GTCAAAAACT TGGAGTCACT 
     5461 TTATTTCTCT AACAACAAGC TCGAATCCTT ACCAGTGGCA GTATTTAGTT TACAGAAACT 
     5521 CAGATGCTTA GATGTGAGCT ACAACAACAT TTCAATGATT CCAATAGAAA TAGGATTGCT 
     5581 TCAGAACCTG CAGCATTTGC ATATCACTGG GAACAAAGTG GACATTCTGC CAAAACAATT 
     5641 GTTTAAATGC ATAAAGTTGA GGACTTTGAA TCTGGGACAG AACTGCATCA CCTCACTCCC 
     5701 AGAGAAAGTT GGTCAGCTCT CCCAGCTCAC TCAGCTGGAG CTGAAGGGGA ACTGCTTGGA 
     5761 CCGCCTGCCA GCCCAGCTGG GCCAGTGTCG GATGCTCAAG AAAAGCGGGC TTGTTGTGGA 
     5821 AGATCACCTT TTTGATACCC TGCCACTCGA AGTCAAAGAG GCATTGAATC AAGACATAAA 
     5881 TATTCCCTTT GCAAATGGGA TTACGCGTAC GCGGCCGCTC GAGATGAGCG GGGGCGAGGA 
     5941 GCTGTTCGCC GGCATCGTGC CCGTGCTGAT CGAGCTGGAC GGCGACGTGC ACGGCCACAA 
     6001 GTTCAGCGTG CGCGGCGAGG GCGAGGGCGA CGCCGACTAC GGCAAGCTGG AGATCAAGTT 
     6061 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTGGTGACCA CCCTCTGCTA 
     6121 CGGCATCCAG TGCTTCGCCC GCTACCCCGA GCACATGAAG ATGAACGACT TCTTCAAGAG 
     6181 CGCCATGCCC GAGGGCTACA TCCAGGAGCG CACCATCCAG TTCCAGGACG ACGGCAAGTA 
     6241 CAAGACCCGC GGCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 
     6301 GGGCAAGGAC TTCAAGGAGG ACGGCAACAT CCTGGGCCAC AAGCTGGAGT ACAGCTTCAA 
     6361 CAGCCACAAC GTGTACATCC GCCCCGACAA GGCCAACAAC GGCCTGGAGG CTAACTTCAA 
     6421 GACCCGCCAC AACATCGAGG GCGGCGGCGT GCAGCTGGCC GACCACTACC AGACCAACGT 
     6481 GCCCCTGGGC GACGGCCCCG TGCTGATCCC CATCAACCAC TACCTGAGCA CTCAGACCAA 
     6541 GATCAGCAAG GACCGCAACG AGGCCCGCGA CCACATGGTG CTCCTGGAGT CCTTCAGCGC 
     6601 CTGCTGCCAC ACCCACGGCA TGGACGAGCT GTACAGGTCC GGACTCAGAT AAGTTTAAAC 
     6661 GGCCGGCCGC GGTCTGTACA AGTAGGATTC GTCGAGGGAC CTAATAACTT CGTATAGCAT 
     6721 ACATTATACG AAGTTATACA TGTTTAAGGG TTCCGGTTCC ACTAGGTACA ATTCGATATC 
     6781 AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT 
     6841 AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT 
     6901 ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT 
     6961 TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC 
     7021 GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT 
     7081 TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA 
     7141 GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT 
     7201 CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC 
     7261 CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT 
     7321 CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG 
     7381 CATCGATACC GTCGACCTCG ATCGAGACCT AGAAAAACAT GGAGCAATCA CAAGTAGCAA 
     7441 TACAGCAGCT ACCAATGCTG ATTGTGCCTG GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG 
     7501 TTTTCCAGTC ACACCTCAGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT 
     7561 TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA 
     7621 AGATATCCTT GATCTGTGGA TCTACCACAC ACAAGGCTAC TTCCCTGATT GGCAGAACTA 
     7681 CACACCAGGG CCAGGGATCA GATATCCACT GACCTTTGGA TGGTGCTACA AGCTAGTACC 
     7741 AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA TGAAGGAGAG AACACCCGCT TGTTACACCC 
     7801 TGTGAGCCTG CATGGGATGG ATGACCCGGA GAGAGAAGTA TTAGAGTGGA GGTTTGACAG 
     7861 CCGCCTAGCA TTTCATCACA TGGCCCGAGA GCTGCATCCG GACTGTACTG GGTCTCTCTG 
     7921 GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC 
     7981 TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG 
     8041 TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GCATGTGAGC 
     8101 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     8161 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     8221 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     8281 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     8341 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     8401 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     8461 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     8521 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     8581 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     8641 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     8701 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     8761 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGATTAC 
     8821 GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG 
     8881 AAGCCATCAC AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT 
     8941 TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG 
     9001 TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA 
     9061 ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT 
     9121 ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA 
     9181 GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG 
     9241 TCTTTCATTG CCATACGGAA CTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA 
     9301 AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC 
     9361 AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT 
     9421 TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATACTC 
     9481 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     9541 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     9601 CCACCTGAC
//