LOCUS       RC203449L1                8346 bp    DNA     circular  SYN 
DEFINITION  C18orf8 (Myc-DDK-tagged)-Human chromosome 18 open reading frame 8 (C18orf8)
ACCESSION   NM_013326
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6637..6817 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    7545..8204 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    6976..7544 
                     /label="ColE1"  
     misc_feature    5512..5532 
                     /label="LR50 primer"  
     misc_feature    5309..5380 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    5521..6112 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5291..5297 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5291 
                     /label="RMC1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGCGAGGAG GACTACTATC TGGAGCTGTG 
     3361 CGAGCGGCCG GTGCAGTTCG AGAAGGCGAA CCCTGTCAAC TGCGTCTTCT TCGATGAGGC 
     3421 CAACAAGCAG GTTTTTGCTG TTCGATCTGG TGGAGCTACT GGCGTGGTAG TTAAAGGCCC 
     3481 AGATGATAGG AATCCCATCT CATTTAGAAT GGATGACAAA GGAGAAGTGA AGTGCATTAA 
     3541 GTTTTCCTTA GAAAATAAGA TATTGGCTGT TCAGAGGACC TCAAAGACTG TGGATTTTTG 
     3601 TAATTTTATC CCTGATAATT CCCAGCTGGA ATACACACAG GAGTGCAAGA CTAAGAATGC 
     3661 CAACATTCTA GGATTCTGCT GGACTAGTTC AACTGAAATT GTCTTCATAA CAGATCAAGG 
     3721 AATCGAATTT TACCAGGTAT TACCAGAGAA ACGGAGTCTG AAACTCTTGA AGAGCCACAA 
     3781 TCTCAATGTG AATTGGTACA TGTACTGCCC CGAGAGCGCC GTGATCTTGC TGTCTACCAC 
     3841 GGTCCTGGAG AATGTCCTGC AGCCTTTTCA CTTTAGGGCT GGCACTATGT CGAAGCTGCC 
     3901 CAAATTTGAG ATTGAATTAC CAGCTGCGCC TAAGTCAACT AAACCCAGCC TTTCCGAAAG 
     3961 AGACATCGCA ATGGCTACCA TATACGGGCA GCTGTATGTT CTCTTCTTGA GGCATCATTC 
     4021 TCGGACCTCC AACAGCACAG GAGCGGAGGT GGTCCTCTAT CATCTACCAC GAGAAGGTGC 
     4081 CTGTAAAAAG ATGCACATAT TGAAGTTAAA TAGGACGGGA AAGTTTGCCC TGAACGTGGT 
     4141 GGACAACCTG GTAGTCGTGC ATCATCAGGA TACAGAGACA TCGGTAATAT TCGATATCAA 
     4201 GTTACGGGGA GAGTTTGACG GCTCCGTTAC CTTCCACCAC CCCGTGCTTC CCGCTCGATC 
     4261 GATCCAGCCC TATCAGATCC CCATCACAGG TCCTGCTGCC GTGACCAGCC AGTCTCCTGT 
     4321 TCCATGTAAA CTCTATTCTT CATCTTGGAT TGTCTTTCAA CCTGACATCA TTATCAGCGC 
     4381 AAGCCAAGGT TACCTCTGGA ACCTCCAAGT GAAACTTGAG CCCATAGTAA ATCTCTTACC 
     4441 AGACAAAGGA AGACTCATGG ACTTTCTCCT CCAGAGAAAG GAATGCAAGA TGGTCATCCT 
     4501 GTCTGTCTGT TCACAGATGT TAAGTGAGTC AGACAGAGCA TCGCTGCCCG TGATAGCCAC 
     4561 TGTTTTTGAT AAACTCAACC ATGAGTATAA AAAGTACCTG GATGCCGAGC AGAGTTATGC 
     4621 GATGGCGGTG GAAGCAGGGC AGAGCCGAAG CAGCCCGCTC CTCAAGAGGC CGGTGCGGAC 
     4681 CCAGGCGGTG CTGGACCAGT CAGATGTGTA CACCCATGTC CTGTCAGCCT TTGTGGAAAA 
     4741 GAAGGAGATG CCTCATAAAT TTGTGATAGC CGTGCTGATG GAATACATTC GTTCTCTTAA 
     4801 CCAGTTTCAG ATTGCAGTAC AGCATTACCT ACATGAACTT GTTATCAAAA CCCTTGTCCA 
     4861 GCACAACCTC TTTTATATGC TGCATCAGTT CCTGCAGTAC CACGTCCTCA GCGACTCCAA 
     4921 ACCTTTGGCT TGTCTGCTGT TATCCCTAGA GAGTTTCTAT CCTCCTGCTC ATCAGCTATC 
     4981 TCTGGACATG CTGAAGCGAC TTTCAACAGC AAATGATGAA ATAGTAGAAG TTCTCCTTTC 
     5041 CAAACACCAA GTGTTAGCTG CCTTAAGGTT TATCCGGGGC ATTGGTGGCC ATGACAACAT 
     5101 TTCTGCACGA AAATTTTTAG ATGCTGCAAA GCAGACTGAA GACAACATGC TTTTCTATAC 
     5161 AATATTCCGC TTTTTTGAAC AGCGAAACCA GCGTTTGCGA GGGAGCCCCA ATTTCACACC 
     5221 AGGGGAACAC TGTGAAGAAC ATGTTGCTTT TTTCAAACAG ATTTTTGGAG ACCAAGCTCT 
     5281 AATGAGGCCT ACAACATTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA 
     5341 GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC 
     5401 CGGCCGCGGT CTGTACAAGT AGGATTCGTC GAGGGACCTA ATAACTTCGT ATAGCATACA 
     5461 TTATACGAAG TTATACATGT TTAAGGGTTC CGGTTCCACT AGGTACAATT CGATATCAAG 
     5521 CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC 
     5581 TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT 
     5641 GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT 
     5701 GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA 
     5761 ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC 
     5821 CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG 
     5881 GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT 
     5941 TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT 
     6001 TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT 
     6061 CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT 
     6121 CGATACCGTC GACCTCGATC GAGACCTAGA AAAACATGGA GCAATCACAA GTAGCAATAC 
     6181 AGCAGCTACC AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG AGGTGGGTTT 
     6241 TCCAGTCACA CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG 
     6301 CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA 
     6361 TATCCTTGAT CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTGGC AGAACTACAC 
     6421 ACCAGGGCCA GGGATCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC TAGTACCAGT 
     6481 TGAGCAAGAG AAGGTAGAAG AAGCCAATGA AGGAGAGAAC ACCCGCTTGT TACACCCTGT 
     6541 GAGCCTGCAT GGGATGGATG ACCCGGAGAG AGAAGTATTA GAGTGGAGGT TTGACAGCCG 
     6601 CCTAGCATTT CATCACATGG CCCGAGAGCT GCATCCGGAC TGTACTGGGT CTCTCTGGTT 
     6661 AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA 
     6721 ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA 
     6781 CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGCA TGTGAGCAAA 
     6841 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6901 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6961 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     7021 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     7081 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     7141 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     7201 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     7261 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     7321 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     7381 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     7441 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     7501 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGATTACGCC 
     7561 CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG 
     7621 CCATCACAAA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC 
     7681 GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT 
     7741 AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA 
     7801 AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG 
     7861 TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT 
     7921 TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT 
     7981 TTCATTGCCA TACGGAACTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG 
     8041 GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC 
     8101 TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA 
     8161 CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATACTCTTC 
     8221 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8281 GAATGTATTT AGAAAAATAA ACAAATAGGG GTCCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8341 CCTGAC
//