LOCUS       RC203447                6843 bp    DNA     circular  SYN 
DEFINITION  NGLY1 (Myc-DDK-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 1
ACCESSION   NM_018297
VERSION     NM_018297.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3000..3091 
                     /label="Myc-DDK"  
     misc_feature    3121..3706 
                     /label="PolyA signal"  
     misc_feature    3178..3197 
                     /label="Restriction Enzyme"  
     misc_feature    3982..4654 
                     /label="ColE1"  
     misc_feature    5084..5878 
                     /label="Kan/Neo"  
     misc_feature    5913..6215 
                     /label="SV40 ori"  
     misc_feature    6301..6778 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2982..2988 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2982 
                     /label="NGLY1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGCG GCATTGGGCA GCTCCTCAGG CTCGGCGTCC CCGGCCGTGG 
     1081 CTGAGCTCTG CCAGAACACC CCGGAGACCT TTTTGGAGGC CTCCAAGCTG CTGCTCACCT 
     1141 ATGCTGACAA CATCCTCAGA AACCCTAATG ATGAAAAATA TAGATCCATC CGGATTGGAA 
     1201 ACACAGCCTT TTCTACTAGA CTCTTGCCTG TCAGAGGAGC TGTTGAATGT TTATTTGAAA 
     1261 TGGGCTTTGA AGAGGGAGAA ACACATCTCA TCTTTCCTAA AAAAGCTTCA GTGGAGCAGC 
     1321 TGCAAAAAAT TCGTGACCTG ATTGCCATAG AGAGAAGTAG CAGACTGGAT GGCTCAAATA 
     1381 AGAGCCACAA AGTAAAGTCA TCTCAGCAAC CTGCAGCCAG TACCCAGCTT CCTACAACAC 
     1441 CATCTTCAAA TCCCAGTGGG TTAAACCAGC ACACAAGGAA CCGTCAAGGG CAGTCATCAG 
     1501 ATCCACCATC TGCTTCAACG GTTGCTGCTG ACTCAGCCAT TCTAGAAGTT CTTCAGTCCA 
     1561 ACATTCAGCA TGTGCTGGTC TATGAAAATC CTGCTCTTCA GGAGAAAGCG TTGGCTTGTA 
     1621 TTCCGGTCCA AGAACTAAAA AGGAAATCAC AAGAAAAGTT ATCGAGAGCT AGAAAATTGG 
     1681 ATAAAGGTAT CAATATAAGT GATGAGGATT TTCTTTTGCT GGAGCTTTTG CACTGGTTTA 
     1741 AGGAAGAATT TTTTCACTGG GTGAATAACG TTTTGTGCAG CAAATGTGGT GGACAGACTA 
     1801 GGTCTAGAGA TAGATCATTA CTGCCCAGTG ATGATGAGCT GAAGTGGGGT GCAAAGGAAG 
     1861 TGGAAGATCA TTACTGTGAT GCCTGCCAGT TCAGCAATCG ATTCCCAAGA TATAATAACC 
     1921 CTGAGAAACT TTTGGAAACA AGATGTGGAC GGTGTGGCGA GTGGGCCAAT TGTTTTACAC 
     1981 TGTGCTGCCG AGCTGTAGGG TTTGAAGCTC GCTATGTTTG GGATTACACA GACCATGTCT 
     2041 GGACAGAAGT CTATTCTCCT TCTCAGCAGC GGTGGCTGCA CTGTGATGCA TGTGAAGATG 
     2101 TCTGTGACAA GCCACTCCTT TATGAAATAG GATGGGGCAA GAAGCTTTCC TATGTCATAG 
     2161 CATTTTCAAA AGATGAGGTA GTTGATGTCA CTTGGCGATA TTCCTGCAAA CATGAAGAGG 
     2221 TGATTGCCAG AAGAACTAAG GTTAAAGAAG CATTACTTCG AGACACTATT AATGGGCTTA 
     2281 ATAAGCAGAG GCAACTGTTT TTGTCAGAAA ACAGAAGGAA AGAACTTCTC CAGAGGATAA 
     2341 TTGTGGAGCT TGTTGAATTT ATATCTCCCA AAACCCCTAA ACCTGGAGAA CTTGGGGGAA 
     2401 GAATATCTGG GTCAGTGGCT TGGAGAGTAG CCCGAGGTGA AATGGGTCTA CAGAGAAAAG 
     2461 AAACCTTGTT TATTCCCTGT GAAAATGAGA AGATTTCTAA ACAGCTCCAC CTTTGTTACA 
     2521 ATATTGTGAA AGATCGTTAT GTTCGAGTTT CAAATAACAA TCAAACCATT TCTGGATGGG 
     2581 AGAATGGCGT GTGGAAAATG GAATCTATAT TCAGAAAAGT TGAAACAGAC TGGCACATGG 
     2641 TATATTTGGC CCGAAAGGAA GGATCATCTT TTGCTTATAT TTCCTGGAAG TTTGAGTGTG 
     2701 GGTCAGTTGG CCTAAAAGTA GATAGCATTT CTATTAGAAC AAGTAGTCAA ACTTTTCAGA 
     2761 CTGGAACAGT AGAATGGAAA TTGCGATCTG ATACAGCACA AGTAGAACTG ACAGGCGATA 
     2821 ACAGTCTTCA CTCCTATGCT GATTTTTCTG GTGCCACTGA AGTTATTTTG GAAGCAGAAT 
     2881 TAAGCAGAGG AGATGGTGAT GTCGCTTGGC AACACACCCA GCTGTTTAGA CAAAGCTTAA 
     2941 ATGACCATGA AGAAAATTGT TTGGAGATAA TTATAAAATT CAGTGACCTT ACGCGTACGC 
     3001 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3061 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3121 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3181 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3241 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3301 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     3361 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     3421 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     3481 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     3541 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     3601 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     3661 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     3721 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     3781 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     3841 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     3901 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     3961 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4021 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4081 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4141 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4201 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4261 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4321 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     4381 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     4441 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     4501 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     4561 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     4621 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     4681 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     4741 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     4801 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     4861 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     4921 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     4981 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5041 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5101 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5161 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5221 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5281 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     5341 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     5401 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     5461 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     5521 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     5581 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     5641 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     5701 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     5761 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     5821 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     5881 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     5941 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6001 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6061 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6121 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6181 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6241 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6301 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     6361 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     6421 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     6481 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     6541 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     6601 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     6661 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     6721 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     6781 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     6841 TTT
//