LOCUS       RC203429                7374 bp    DNA     circular  SYN 
DEFINITION  SORT1 (Myc-DDK-tagged)-Human sortilin 1 (SORT1), transcript variant 1
ACCESSION   NM_002959
VERSION     NM_002959.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3531..3622 
                     /label="Myc-DDK"  
     misc_feature    3652..4237 
                     /label="PolyA signal"  
     misc_feature    3709..3728 
                     /label="Restriction Enzyme"  
     misc_feature    4513..5185 
                     /label="ColE1"  
     misc_feature    5615..6409 
                     /label="Kan/Neo"  
     misc_feature    6444..6746 
                     /label="SV40 ori"  
     misc_feature    6832..7309 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3513..3519 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3513 
                     /label="SORT1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGGCCC TGGGGAGCTG CGGACGGCCT CTCGCGCTGG CCCCATGGCC 
     1081 TCGGCCTCCT CCTCCTCCTG CAGCTGCTGC CGCCGTCGAC CCTCAGCCAG GACCGGCTGG 
     1141 ACGCGCCGCC GCCGCCCGCT GCGCCGCTGC CGCGCTGGTC TGGCCCCATC GGGGTGAGCT 
     1201 GGGGGCTGCG GGCGGCCGCA GCCGGGGGCG CGTTTCCCCG CGGCGGCCGT TGGCGTCGCA 
     1261 GCGCGCCGGG CGAGGACGAG GAGTGCGGCC GGGTCCGGGA CTTCGTCGCC AAGCTGGCCA 
     1321 ACAACACGCA CCAGCATGTG TTTGATGATC TCAGAGGCTC AGTATCCTTG TCCTGGGTTG 
     1381 GAGATAGCAC TGGGGTCATT CTAGTCTTGA CTACCTTCCA TGTACCACTG GTAATTATGA 
     1441 CTTTTGGACA GTCCAAGCTA TATCGAAGTG AGGATTATGG GAAGAACTTT AAGGATATTA 
     1501 CAGATCTCAT CAATAACACC TTTATTCGGA CTGAATTTGG CATGGCTATT GGTCCTGAGA 
     1561 ACTCTGGAAA GGTGGTGTTA ACAGCAGAGG TGTCTGGAGG AAGTCGTGGA GGAAGAATCT 
     1621 TCAGATCATC AGATTTTGCG AAGAATTTTG TGCAAACAGA TCTCCCTTTT CATCCTCTCA 
     1681 CTCAGATGAT GTATAGCCCT CAGAATTCTG ATTATCTTTT AGCTCTCAGC ACTGAAAATG 
     1741 GCCTGTGGGT GTCCAAGAAT TTTGGGGGAA AATGGGAAGA AATCCACAAA GCAGTATGTT 
     1801 TGGCCAAATG GGGATCAGAC AACACCATCT TCTTTACAAC CTATGCAAAT GGCTCCTGCA 
     1861 AAGCTGACCT TGGGGCTCTG GAATTATGGA GAACTTCAGA CTTGGGAAAA AGCTTCAAAA 
     1921 CTATTGGTGT GAAAATCTAC TCATTTGGTC TTGGGGGACG TTTCCTTTTT GCCTCTGTGA 
     1981 TGGCTGATAA GGATACCACA AGAAGGATCC ACGTTTCAAC AGATCAAGGG GACACATGGA 
     2041 GCATGGCCCA GCTCCCCTCC GTGGGACAGG AACAGTTCTA TTCTATTCTG GCAGCAAATG 
     2101 ATGACATGGT ATTCATGCAT GTAGATGAAC CTGGAGACAC TGGGTTTGGC ACAATCTTTA 
     2161 CCTCAGATGA TCGAGGCATT GTCTATTCCA AGTCTTTGGA CCGACATCTC TACACTACCA 
     2221 CAGGCGGAGA GACGGACTTT ACCAACGTGA CCTCCCTCCG CGGCGTCTAC ATAACAAGCG 
     2281 TGCTCTCCGA AGATAATTCT ATCCAGACCA TGATCACTTT TGACCAAGGA GGAAGGTGGA 
     2341 CGCACCTGAG GAAGCCTGAA AACAGTGAAT GTGATGCTAC AGCAAAAAAC AAGAATGAGT 
     2401 GCAGCCTTCA TATTCATGCT TCCTACAGCA TCTCCCAGAA ACTGAATGTT CCAATGGCCC 
     2461 CACTCTCAGA GCCGAATGCC GTAGGCATTG TCATTGCTCA TGGTAGCGTG GGGGATGCCA 
     2521 TCTCAGTGAT GGTTCCAGAT GTGTACATCT CAGATGATGG GGGTTACTCC TGGACAAAGA 
     2581 TGCTGGAAGG ACCCCACTAT TACACCATCC TGGATTCTGG AGGCATCATT GTGGCCATTG 
     2641 AGCACAGCAG CCGTCCTATC AATGTGATTA AGTTCTCCAC AGACGAAGGT CAATGCTGGC 
     2701 AAACCTACAC GTTCACCAGG GACCCCATCT ATTTCACTGG CCTAGCTTCA GAACCTGGAG 
     2761 CTAGGTCCAT GAATATCAGC ATTTGGGGCT TCACAGAATC TTTCCTGACC AGCCAGTGGG 
     2821 TCTCCTACAC CATTGATTTT AAAGATATCC TTGAAAGGAA CTGTGAAGAG AAGGACTATA 
     2881 CCATATGGCT GGCACACTCC ACAGACCCTG AAGATTATGA AGATGGCTGC ATTTTGGGCT 
     2941 ACAAAGAACA GTTTCTGCGG CTACGCAAGT CATCCGTGTG TCAGAATGGT CGAGACTATG 
     3001 TTGTGACCAA GCAGCCCTCC ATCTGCCTCT GTTCCCTGGA GGACTTTCTC TGTGATTTTG 
     3061 GCTACTACCG TCCAGAAAAT GACTCCAAGT GTGTGGAACA GCCAGAACTG AAGGGCCACG 
     3121 ACCTGGAGTT TTGTCTGTAC GGAAGAGAAG AACACCTAAC AACAAATGGG TACCGGAAAA 
     3181 TTCCAGGGGA CAAATGCCAG GGTGGGGTAA ATCCAGTTCG AGAAGTAAAA GACTTGAAAA 
     3241 AGAAATGCAC AAGCAACTTT TTGAGTCCGG AAAAACAGAA TTCCAAGTCA AATTCTGTTC 
     3301 CAATTATCCT GGCCATCGTG GGATTGATGC TGGTCACAGT CGTAGCAGGA GTGCTCATTG 
     3361 TGAAGAAATA TGTCTGTGGG GGAAGGTTCC TGGTGCATCG ATACTCTGTG CTGCAGCAGC 
     3421 ATGCAGAGGC CAATGGTGTG GATGGTGTGG ATGCTTTGGA CACAGCCTCC CACACTAATA 
     3481 AAAGTGGTTA TCATGATGAC TCAGATGAGG ACCTCTTGGA AACGCGTACG CGGCCGCTCG 
     3541 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3601 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3661 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3721 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3781 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3841 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3901 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3961 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4021 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4081 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4141 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4201 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4261 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4321 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4381 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4441 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4501 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4561 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4621 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4681 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4741 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4801 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4861 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4921 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4981 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5041 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5101 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5161 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5221 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5281 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5341 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5401 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5461 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5521 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5581 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5641 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5701 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5761 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5821 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5881 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5941 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6001 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6061 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6121 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6181 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6241 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6301 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6361 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6421 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6481 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6541 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6601 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6661 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6721 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6781 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6841 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6901 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6961 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7021 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7081 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7141 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7201 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7261 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7321 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//