LOCUS       RC202901                7551 bp    DNA     circular  SYN 
DEFINITION  WFS1 (Myc-DDK-tagged)-Human Wolfram syndrome 1 (wolframin) (WFS1), transcript variant 1
ACCESSION   NM_006005
VERSION     NM_006005.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3708..3799 
                     /label="Myc-DDK"  
     misc_feature    3829..4414 
                     /label="PolyA signal"  
     misc_feature    3886..3905 
                     /label="Restriction Enzyme"  
     misc_feature    4690..5362 
                     /label="ColE1"  
     misc_feature    5792..6586 
                     /label="Kan/Neo"  
     misc_feature    6621..6923 
                     /label="SV40 ori"  
     misc_feature    7009..7486 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3690..3696 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3690 
                     /label="WFS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTCCAAC ACTGCTCCGC TGGGCCCCTC CTGCCCACAG CCCCCGCCAG 
     1081 CACCGCAGCC CCAGGCGCGT TCCCGACTCA ATGCCACAGC CTCGTTGGAG CAGGAGAGGA 
     1141 GCGAAAGGCC CCGAGCACCC GGACCCCAGG CTGGCCCTGG CCCTGGTGTT AGAGACGCAG 
     1201 CGGCCCCCGC TGAACCCCAG GCCCAGCATA CCAGGAGCCG GGAAAGAGCA GACGGCACCG 
     1261 GGCCTACAAA GGGAGACATG GAAATCCCCT TTGAAGAAGT CCTGGAGAGG GCCAAGGCCG 
     1321 GGGACCCCAA GGCACAGACT GAGGTGGGGA AGCACTACCT GCAGTTGGCC GGCGACACGG 
     1381 ATGAAGAACT CAACAGCTGC ACCGCTGTGG ACTGGCTGGT CCTCGCCGCG AAGCAGGGCC 
     1441 GTCGCGAGGC TGTGAAGCTG CTTCGCCGGT GCTTGGCGGA CAGAAGAGGC ATCACGTCCG 
     1501 AGAACGAACG GGAGGTGAGG CAGCTCTCCT CCGAGACCGA CCTGGAGAGG GCCGTGCGCA 
     1561 AGGCAGCCCT GGTCATGTAC TGGAAGCTCA ACCCCAAGAA GAAGAAGCAG GTGGCCGTGG 
     1621 CGGAGCTGCT GGAGAATGTC GGCCAGGTCA ACGAGCACGA TGGAGGGGCG CAGCCAGGCC 
     1681 CCGTGCCCAA GTCCCTGCAG AAGCAGAGGC GCATGCTGGA GCGCCTGGTC AGCAGCGAGT 
     1741 CCAAGAACTA CATCGCGCTG GATGACTTTG TGGAGATCAC TAAGAAGTAC GCCAAGGGCG 
     1801 TCATCCCCAG CAGCCTGTTC CTGCAGGACG ACGAAGATGA TGACGAGCTG GCGGGGAAGA 
     1861 GCCCTGAGGA CCTGCCACTG CGTCTGAAGG TGGTCAAGTA CCCCCTGCAC GCCATCATGG 
     1921 AGATCAAGGA GTACCTGATT GACATGGCCT CCAGGGCAGG CATGCACTGG CTGTCCACCA 
     1981 TCATCCCCAC GCACCACATC AACGCGCTCA TCTTCTTCTT CATCGTCAGC AACCTCACCA 
     2041 TCGACTTCTT CGCCTTCTTC ATCCCGCTGG TCATCTTCTA CCTGTCCTTC ATCTCCATGG 
     2101 TGATCTGCAC CCTCAAGGTG TTCCAGGACA GCAAGGCCTG GGAGAACTTC CGCACCCTCA 
     2161 CCGACCTGCT GCTGCGCTTC GAGCCCAACC TGGATGTGGA GCAGGCCGAG GTCAACTTCG 
     2221 GCTGGAACCA CCTGGAGCCC TATGCCCATT TCCTGCTCTC TGTCTTCTTC GTCATCTTCT 
     2281 CCTTCCCCAT CGCCAGCAAG GACTGCATCC CCTGCTCGGA GCTGGCTGTC ATCACCGGCT 
     2341 TCTTTACCGT GACCAGCTAC CTGAGCCTGA GCACCCATGC AGAGCCCTAC ACGCGCAGGG 
     2401 CCCTGGCCAC CGAGGTCACC GCCGGCCTGC TATCGCTGCT GCCCTCCATG CCCTTGAATT 
     2461 GGCCCTACCT GAAGGTCCTT GGCCAGACCT TCATCACCGT GCCTGTCGGC CACCTGGTCG 
     2521 TCCTCAACGT CAGCGTCCCG TGCCTGCTCT ATGTCTACCT GCTCTATCTC TTCTTCCGCA 
     2581 TGGCACAGCT GAGGAATTTC AAGGGCACCT ACTGCTACCT TGTGCCCTAC CTGGTGTGCT 
     2641 TCATGTGGTG TGAGCTCTCC GTGGTCATCC TGCTGGAGTC CACCGGCCTG GGGCTGCTCC 
     2701 GCGCCTCCAT CGGCTACTTC CTCTTCCTCT TTGCCCTCCC CATCCTGGTG GCCGGCCTGG 
     2761 CCCTGGTGGG CGTGCTGCAG TTCGCCCGGT GGTTCACGTC TCTGGAGCTC ACCAAGATCG 
     2821 CAGTCACCGT GGCGGTCTGT AGTGTGCCCC TGCTGTTGCG CTGGTGGACC AAGGCCAGCT 
     2881 TCTCTGTGGT GGGGATGGTG AAGTCCCTGA CGCGGAGCTC CATGGTCAAG CTCATCCTGG 
     2941 TGTGGCTCAC GGCCATCGTG CTGTTCTGCT GGTTCTATGT GTACCGCTCA GAGGGCATGA 
     3001 AGGTCTACAA CTCCACACTG ACCTGGCAGC AGTATGGTGC GCTGTGCGGG CCACGCGCCT 
     3061 GGAAGGAGAC CAACATGGCG CGCACCCAGA TCCTCTGCAG CCACCTGGAG GGCCACAGGG 
     3121 TCACGTGGAC CGGCCGCTTC AAGTACGTCC GCGTGACTGA CATCGACAAC AGCGCCGAGT 
     3181 CTGCCATCAA CATGCTCCCG TTCTTCATCG GCGACTGGAT GCGCTGCCTC TACGGCGAGG 
     3241 CCTACCCTGC CTGCAGCCCT GGCAACACCT CCACGGCCGA GGAGGAGCTC TGTCGCCTTA 
     3301 AGCTGCTGGC CAAGCACCCC TGCCACATCA AGAAGTTCGA CCGCTACAAG TTTGAGATTA 
     3361 CCGTGGGCAT GCCATTCAGC AGCGGCGCTG ACGGCTCGCG CAGCCGCGAG GAGGACGACG 
     3421 TCACCAAGGA CATCGTGCTG CGGGCCAGCA GCGAGTTCAA GAGCGTGCTG CTCAGCCTGC 
     3481 GCCAGGGCAG CCTCATCGAG TTCAGCACCA TCCTGGAGGG CCGCCTGGGC AGCAAGTGGC 
     3541 CTGTCTTCGA GCTCAAGGCC ATCAGCTGCC TCAACTGCAT GGCCCAGCTC TCACCCACCA 
     3601 GGCGGCACGT GAAGATCGAG CACGACTGGC GCAGCACCGT GCATGGCGCC GTGAAGTTCG 
     3661 CCTTCGACTT CTTTTTCTTC CCATTCCTGT CGGCGGCCAC GCGTACGCGG CCGCTCGAGC 
     3721 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3781 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3841 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3901 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3961 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4021 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4081 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4141 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4201 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4261 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4321 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4381 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4441 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4501 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4561 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4621 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4681 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4741 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4801 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4861 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4921 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4981 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5041 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5101 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5161 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5221 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5281 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5341 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5401 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5461 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5521 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5581 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5641 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5701 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5761 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5821 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5881 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5941 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6001 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6061 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6121 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6181 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6241 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6301 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6361 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6421 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6481 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6541 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6601 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6661 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6721 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6781 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6841 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6901 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6961 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7021 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7081 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7141 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7201 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7261 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7321 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7381 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7441 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7501 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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